scholarly journals The Phytochemical Analysis of Hay Infusions and Papaya Leaf Juice as an Attractant Containing Insecticide for Aedes Aegypti

2017 ◽  
Vol 12 (2) ◽  
pp. 218-224
Author(s):  
Widya Hary Cahyati ◽  
Widya Asmara ◽  
S Rahmah Umniyati ◽  
Budi Mulyaningsih
Keyword(s):  
Lactic Acid ◽  
Aedes Aegypti ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chemical Compounds ◽  
Hplc Method ◽  
Phytochemical Analysis ◽  
Layer Chromatography ◽  
Leaf Juice

Aedes aegypti mosquito population could be controlled by using lethal ovitrap. The addition of hay infusions as a attractant greatly enhance Aedes aegypti eggs trapped, and papaya leaf juice may inhibit Aedes aegypti eggs evolve to larvae or a larvae to pupae stage. This study was conducted to find out the chemical compounds in hay infusion and papaya leaf juice. We used phytochemical test using UV-Vis Spectrophotometry, Thin Layer Chromatography, and High Performance Liquid Chromatoraphy (HPLC) method. The results showed that hay infusion contains 12,75 mg/L ammonium and <1,20 ppm (g/mL) lactic acid and papaya leaf juice contains 0,25% alkaloid, 0,14% flavonoid, 0,30% saponin, ?68 mg/L steroid and 11,34% tannin, but negative terpenoid. We concluded that hay infusion and papaya leaf juice contains chemical compounds that could be use as attractant and bioinsecticide to Aedes aegypti, respectively.

scholarly journals Simultaneous Estimation of Pantoprazole and Domperidone in Pure Powder and a Pharmaceutical Formulation by High-Perfomance Liquid Chromatography and High-Performance Thin-Layer Chromatography Methods

2007 ◽  
Vol 90 (1) ◽  
pp. 142-146 ◽  
Author(s):  
Bhavesh H Patel ◽  
Bhanubhai N Suhagia ◽  
Madhabhai M Patel ◽  
Jignesh R Patel
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Uv Detection ◽  
Simultaneous Estimation ◽  
Phase Quantification ◽  
Layer Chromatography

Abstract This paper describes validated high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods for the simultaneous estimation of pantoprazole (PANT) and domperidone (DOM) in pure powder and capsule formulations. The HPLC separation was achieved on a Phenomenex C18 column (250 mm id, 4.6 mm, 5 μm) using 0.01 M, 6.5 pH ammonium acetate buffer-methanol-acetonitrile (30 + 40 + 30, v/v/v, pH 7.20) as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using ethyl acetatemethanol (60 + 40, v/v) as the mobile phase. Quantification was achieved with ultraviolet (UV) detection at 287 nm over the concentration range 400-4000 and 300-3000 ng/mL with mean recovery of 99.35 ± 0.80 and 99.08 ± 0.57% for PANT and DOM, respectively (HPLC method). Quantification was achieved with UV detection at 287 nm over the concentration range 80-240 and 60-180 ng/spot with mean recovery of 98.40 ± 0.67 and 98.75 ± 0.71% for PANT and DOM, respectively (HPTLC method). These methods are simple, precise, and sensitive, and they are applicable for the simultaneous determination of PANT and DOM in pure powder and capsule formulations.

scholarly journals PHARMACOGNOSTICAL STANDARDIZATION AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINT OF ROOTS OF ANTHOCEPHALUS CADAMBA

2019 ◽  
pp. 194-198
Author(s):  
KAVERIPAKAM SAISRUTHI ◽  
ADIKAY SREEDEVI
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Ethanol Extract ◽  
Physicochemical Analysis ◽  
Phytochemical Analysis ◽  
Vessel Elements ◽  
Layer Chromatography ◽  
Anthocephalus Cadamba

Objective: Anthocephalus cadamba is a traditional medicinal plant which is valued for its benefits in the management of various ailments. The current study was aimed to provide requisite pharmacognostic details and high-performance thin-layer chromatography (HPTLC) fingerprint profile for the roots of A. cadamba. Methods: Pharmacognostic evaluation included examination of morphological and microscopic characters, physicochemical analysis, fluorescence study, preliminary phytochemical analysis, and HPTLC fingerprint. Results: The microscopic studies showed the presence of thick periderm, cortical zone, secondary phloem, and secondary xylem. Microscopic examination of root powder showed abundant fibers and vessel elements. The physicochemical analysis of root powder helped in the assessment of quality control parameters. Fluorescence analysis of root powder imparted characteristic colors on the treatment of the powder with different reagents. Preliminary phytochemical studies reported the presence of alkaloids, saponins, flavonoids, steroids, terpenoids, glycosides, tannins, and other phenolic compounds. The developed qualitative densitometric HPTLC fingerprint can be used as an identifying biochemical marker for ethanol extract of the roots of A. cadamba. Conclusion: The present study provides the information with respect to identification and authentication of crude drug and serves as a reference point for the proper identification of roots of this medicinal plant, thereby contributing to the scientific world of research.

Production of Biogenic Amines by Lactic Acid Bacteria: Screening by PCR, Thin-Layer Chromatography, and High-Performance Liquid Chromatography of Strains Isolated from Wine and Must

2006 ◽  
Vol 69 (2) ◽  
pp. 391-396 ◽  
Author(s):  
ANTONELLA COSTANTINI ◽  
MANUELA CERSOSIMO ◽  
VINCENZO DEL PRETE ◽  
EMILIA GARCIA-MORUNO
Keyword(s):  
High Performance Liquid Chromatography ◽  
Lactic Acid ◽  
Liquid Chromatography ◽  
Lactic Acid Bacteria ◽  
Thin Layer ◽  
Biogenic Amines ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Histidine Decarboxylase ◽  
Layer Chromatography

Biogenic amines are frequently found in wine and other fermented food. We investigated the ability of 133 strains of lactic acid bacteria isolated from musts and wines of different origins to produce histamine, tyramine, and putrescine. We detected the genes responsible for encoding the corresponding amino acid decarboxylases through PCR assays using two primer sets for every gene: histidine decarboxylase (hdc), tyrosine decarboxylase (tdc), and ornithine decarboxylase (odc); these primers were taken from the literature or designed by us. Only one strain of Lactobacillus hilgardii was shown to possess the hdc gene, whereas four strains of Lactobacillus brevis had the tdc gene. None of the Oenococcus oeni strains, the main agents of malolactic fermentation, was a biogenic amine producer. All PCR amplicon band–positive results were confirmed by thin-layer chromatography and high-performance liquid chromatography analyses.

scholarly journals PHYTOCHEMICAL EVALUATION AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY PROFILING: SAPINDUS EMARGINATUS VAHL. AND MORINDA PUBESCENS J.E.SM. BARKS EXTRACTS

2016 ◽  
Vol 9 (9) ◽  
pp. 108 ◽  
Author(s):  
Hariba Dhondiram Jedage ◽  
Manjunath Kp
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Flavonoid Compound ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Rf Values ◽  
Ethanol Extracts ◽  
Morinda Pubescens ◽  
Layer Chromatography

ABSTRACTObjective: To identify the flavonoids high-performance thin layer chromatography (HPTLC) profiles from barks of Sapindus emarginatus Vahl.(Sapindaceae) and Morinda pubescens J.E.Sm. (Rubiaceae) by ethanol extracts.Methods: The barks of S. emarginatus Vahl. and M. pubescens J.E.Sm. extracted each separately with ethanol. Both these ethanol extracts weresubjected to preliminary phytochemical analysis. Based on phytochemical studies, the extracts obtained were subjected to HPTLC profiles for identifyand confirmation flavonoids, both these samples were compared with standard (Rutin). HPTLC analysis performed with silica gel G 60 F254 plateswith mobile phase ethyl acetate:n-butanol:formic acid:water (5:3:1:1). Detection of flavonoid compound was performed by scanning the developedplate at 254 nm.Results: Result of these both extracts shows positive tests for flavonoids. Ethanol extracts barks of S. emarginatus Vahl. and M. pubescens J.E.Sm eachwere showed bands of different Rf values with range 0.50-0.90 and standard showed bands of 0.50 Rf values.Conclusion: It can be concluded that rutin constituents of each bark extract are presents and have effective components which can be utilized as auseful herb for alleviation of various illness and disorders.Keywords: Sapindus emarginatus Vahl., Morinda pubescens J.E.Sm. Ethanol extracts, High-performance thin layer chromatography, Rutin.

scholarly journals HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) FINGERPRINTING PATTERN OF MANGROVE AVICENNIA MARINA

2017 ◽  
Vol 9 (4) ◽  
pp. 65
Author(s):  
Vinars Dawane ◽  
M. H. Fulekar
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Avicennia Marina ◽  
Phytochemical Analysis ◽  
Specific Marker ◽  
Root Extract ◽  
Mangrove Species ◽  
Mangrove Plant ◽  
Layer Chromatography

Objective: An attempt has been made to study phytoconstituents and High-Performance Thin Layer Chromatography (HPTLC) fingerprinting pattern of leaf/stem/root methanol extracts of Avicennia marina.Methods: The phytochemical screening was done by standard biochemical methods and standard optimized HPTLC densitometry determination was performed under two different energy zones [under UV-254 nm and under 540 nm after derivatization at sample size-10 µl, temperature = 25.8±0.3 °C (constant) and relative humidity = 86±1% (constant) in optimized solvent system].Results: Phytochemical analysis confirmed the presence of phytochemicals in the leaf, stem, and root of the plant. Under UV–254 nm energy range, the leaf, stem and root extract showed the presence of 8, 6 and 5 components respectively and under 540 nm energy zone after derivatization, the leaf, stem and root extract revealed the presence of 7, 5 and 5 components, respectively and specific marker components with very high concentration (under UV-254 nm; 3, 2, 2 in leaf, stem, and root respectively and under 540 nm after ASR treatment; 2, 1, 2 in leaf, stem, and root respectively) were detected among them.Conclusion: The preliminary phytochemical test results elucidated Avicennia marina leaf, stem, and root as a hug reservoir of various class of phytochemicals and metabolites. The results obtained by HPTLC fingerprinting method found to be acceptable as a quick, reliable, accurate and economical for identification and authentication of Avicennia marina mangrove plant and useful to differentiate this mangrove species from other similar mangroves, in a single TLC plate run. This serves as a biochemical marker pattern for leaf, stem and root parts of this mangrove and for its diverse phytoconstituents.

scholarly journals Phytochemical Analysis and Thin Layer Chromatography Profiling of Crude Extracts from Senna Occidentalis(Leaves)

2019 ◽  
Vol 2 (1) ◽  
pp. 12-21 ◽  
Author(s):  
Lawal A.M ◽  
Abdullahi R ◽  
Ibrahim M.S ◽  
Kurfi M.Y ◽  
Khalid A ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Cardiac Glycosides ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Test Methods ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Layer Chromatography

Plants used for medicinal practices which were discovered since prehistoric stone ages are termed Medicinal plants, which are also referred to as medicinal herbs, since plants produces bioactive chemical compounds (phytochemicals), this research however, is concerned with the extraction using Soxhlet extraction technique, phytochemical screening using various test methods, which reveals the presence of anthraquinones (free anthraquinones and combined anthraquinones), carbohydrates, cardiac glycosides, glycosides, flavonoids, saponins, steroids/ terpenes, phenolic compounds and tannins, and absence of alkaloids for extracts of senna occidentalis and also, thin layer chromatography profiling which gives probable foundation for further structural elucidation amongst others. This research shows the presence of potent secondary metabolites present in the leaves of senna occidentalis (leaves).

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