scholarly journals Phytochemical analysis of ethanolic extract of Dichrostachys Cinerea W and Arn leaves by a thin layer chromatography, high performance thin layer chromatography and column chromatography

2013 ◽  
Vol 32 (4) ◽  
pp. 227 ◽  
Author(s):  
M Vijayalakshmi ◽  
K Periyanayagam ◽  
K Kavitha ◽  
K Akilandeshwari
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
High Performance ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Dichrostachys Cinerea ◽  
Layer Chromatography

scholarly journals PHARMACOGNOSTICAL STANDARDIZATION AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINT OF ROOTS OF ANTHOCEPHALUS CADAMBA

2019 ◽  
pp. 194-198
Author(s):  
KAVERIPAKAM SAISRUTHI ◽  
ADIKAY SREEDEVI
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Ethanol Extract ◽  
Physicochemical Analysis ◽  
Phytochemical Analysis ◽  
Vessel Elements ◽  
Layer Chromatography ◽  
Anthocephalus Cadamba

Objective: Anthocephalus cadamba is a traditional medicinal plant which is valued for its benefits in the management of various ailments. The current study was aimed to provide requisite pharmacognostic details and high-performance thin-layer chromatography (HPTLC) fingerprint profile for the roots of A. cadamba. Methods: Pharmacognostic evaluation included examination of morphological and microscopic characters, physicochemical analysis, fluorescence study, preliminary phytochemical analysis, and HPTLC fingerprint. Results: The microscopic studies showed the presence of thick periderm, cortical zone, secondary phloem, and secondary xylem. Microscopic examination of root powder showed abundant fibers and vessel elements. The physicochemical analysis of root powder helped in the assessment of quality control parameters. Fluorescence analysis of root powder imparted characteristic colors on the treatment of the powder with different reagents. Preliminary phytochemical studies reported the presence of alkaloids, saponins, flavonoids, steroids, terpenoids, glycosides, tannins, and other phenolic compounds. The developed qualitative densitometric HPTLC fingerprint can be used as an identifying biochemical marker for ethanol extract of the roots of A. cadamba. Conclusion: The present study provides the information with respect to identification and authentication of crude drug and serves as a reference point for the proper identification of roots of this medicinal plant, thereby contributing to the scientific world of research.

scholarly journals Isolation and Structure Elucidation of Quercetin like Structure from Dalbergia sissoo (Fabaceae)

2020 ◽  
Vol 10 (3-s) ◽  
pp. 6-11
Author(s):  
Rohit Kumar KUMAR Bijauliya ◽  
Pushpendra Kannojia ◽  
Pankaj Mishra ◽  
Gaurav Kumar Pathak
Keyword(s):  
Nmr Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
High Performance ◽  
18Th Century ◽  
Dalbergia Sissoo ◽  
Layer Chromatography

The present study was conducted to isolate and classify Dalbergia sissoo (L.) bioactive compounds. The genus consists of 300 species in India, including 25 species. The generic name Dalbergia honors the 18th century Swedish brothers Nils and Carl Dalberg. Various phytoconstituents of alkaloids, glycosides, flavanoids, tannins, saponins, sterols and terpenoids were isolated and classified from different parts of the plant. Thin Layer Chromatography, High Performance Thin Layer Chromatography, and Column Chromatography were used to isolate spots from the fraction of the crude extract to elucidate the chemical structure of Dalbergia sissoo (L.) leaf extract. Potential spots have been exposed to techniques of FTIR, NMR and mass spectroscopy. Column chromatography was exposed to the raw extract, obtaining 125 fractions, conducting TLC. Among them was a single band in TLC, characterized by FTIR, NMR spectroscopy and mass spectroscopy, and the structure was known as Quercetin. The results of this study indicate the effective potential compound of the ethanolic fraction of Dalbergia sissoo (L.). Keywords: Dalbergia sissoo; Isolation & Structure Elucidation; FTIR; NMR spectroscopy; Mass spectroscopy.

scholarly journals PHYTOCHEMICAL AND PHARMACOGNOSTICAL STUDIES OF ANOGEISSUS ACUMINATA

2019 ◽  
Vol 9 (4-A) ◽  
pp. 450-457
Author(s):  
Vijay Kumar Yadav ◽  
Raghuveer Irchhiaya ◽  
A. K. Ghosh
Keyword(s):  
Organic Matter ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ethanolic Extract ◽  
New Drugs ◽  
Phytochemical Analysis ◽  
Isolation And Identification ◽  
Natural Medicine ◽  
Layer Chromatography

Medicinal plants are one of the oldest valuable sources of medicine provide by nature to mankind. Medicinal plants are one of the important sources of research of new drugs. Many medicinal plants and herbs hold their prestigious position in the field of natural medicine among which Anogeissus acuminata belonging to family Combretaceae was vital one. In the present study was intended to evaluate various physicochemical, phytochemical standards like TLC, HPTLC and Pharmacognostic parameters which will be helpful to ensure the purity, safety and efficacy of the medicinal plant.  Anogeissus acuminata was subjected to preliminary phytochemical screening, done to estimate as foreign organic matter, morphological evaluation. Total ash values, physicochemical, Thin layer chromatography (TLC), HPTLC phytochemical analysis and secondary metabolites present in the plant were investigated for the extract. All physiochemical properties were found within the measureable amount as foreign organic matter less than 1% w/w, Total ash values was 12 % w/w with respect to air-dried crude drug. Moisture content was 3.074. Thin layer chromatography (TLC), HPTLC studies were carried for the better isolation and identification of the different components of ethanolic extract. HPTLC carried out in the solvent system - Toluene: Ethyl acetate: Acetic acid (4:3:1). The report of HPTLC indicates the presence of sixteen spots. Preliminary phytochemical analysis confirmed the presence of plant extracts revealed the presence of alkaloids, steroids, terpenoids glycosides, tannins and phenolic compounds. KEYWORDS: TLC, HPTLC, Phytochemical characterization, Anogeissus acuminata

scholarly journals phytochemical investigations of Iraqi Abrus precatorius Linn. plant

2018 ◽  
Vol 27 (1) ◽  
pp. 30-38
Author(s):  
Zahra'a S. Nassir ◽  
Enas J. Khadem
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Fourier Transforms ◽  
Ethanolic Extract ◽  
Preparative Thin Layer Chromatography ◽  
Chloroform Fraction ◽  
Abrus Precatorius ◽  
Soxhlet Apparatus ◽  
Layer Chromatography

    The plant Abrus precatorius, which belong to Leguminosae (Fabaceae) family and known as Crab’s eyes, Rosary pea with characteristic red and black seeds. It was used in folk medicine in India, China and East Asian countries for treatment of various diseases.    The plant was extracted by '' general method of extraction'' (Harborne, 1973) using 80% aqueous ethanol as a solvent of extraction by soxhlet apparatus. Preliminary qualitative phytochemical screening were performed on the crude ethanolic extract  and revealed the presence of alkaloids, flavonoids ,terpenoids and phytosterols in Iraqi Abrus precatorius plant. Three different fractions were obtained from crude extract which are fraction one (chloroform fraction), fraction two (ethyl acetate fraction), and fraction three (petroleum ether fraction) which are represent alkaloids, flavonoids and steroids respectively. The alkaloid abrine was isolated from the chloroform fraction  in pure form by using preparative thin layer chromatography (PTLC) and then subjected to different physico-chemical and specteral analytical techniques to identify its chemical structure: melting point (M.P.), thin layer chromatography (TLC), high performance liquid chromatography (HPLC) , fourier transforms infrared spectra (FT-IR) and elemental microanalysis (CHNO).      

scholarly journals PHYTOCHEMICAL EVALUATION AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY PROFILING: SAPINDUS EMARGINATUS VAHL. AND MORINDA PUBESCENS J.E.SM. BARKS EXTRACTS

2016 ◽  
Vol 9 (9) ◽  
pp. 108 ◽  
Author(s):  
Hariba Dhondiram Jedage ◽  
Manjunath Kp
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Flavonoid Compound ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Rf Values ◽  
Ethanol Extracts ◽  
Morinda Pubescens ◽  
Layer Chromatography

ABSTRACTObjective: To identify the flavonoids high-performance thin layer chromatography (HPTLC) profiles from barks of Sapindus emarginatus Vahl.(Sapindaceae) and Morinda pubescens J.E.Sm. (Rubiaceae) by ethanol extracts.Methods: The barks of S. emarginatus Vahl. and M. pubescens J.E.Sm. extracted each separately with ethanol. Both these ethanol extracts weresubjected to preliminary phytochemical analysis. Based on phytochemical studies, the extracts obtained were subjected to HPTLC profiles for identifyand confirmation flavonoids, both these samples were compared with standard (Rutin). HPTLC analysis performed with silica gel G 60 F254 plateswith mobile phase ethyl acetate:n-butanol:formic acid:water (5:3:1:1). Detection of flavonoid compound was performed by scanning the developedplate at 254 nm.Results: Result of these both extracts shows positive tests for flavonoids. Ethanol extracts barks of S. emarginatus Vahl. and M. pubescens J.E.Sm eachwere showed bands of different Rf values with range 0.50-0.90 and standard showed bands of 0.50 Rf values.Conclusion: It can be concluded that rutin constituents of each bark extract are presents and have effective components which can be utilized as auseful herb for alleviation of various illness and disorders.Keywords: Sapindus emarginatus Vahl., Morinda pubescens J.E.Sm. Ethanol extracts, High-performance thin layer chromatography, Rutin.

scholarly journals HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) FINGERPRINTING PATTERN OF MANGROVE AVICENNIA MARINA

2017 ◽  
Vol 9 (4) ◽  
pp. 65
Author(s):  
Vinars Dawane ◽  
M. H. Fulekar
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Avicennia Marina ◽  
Phytochemical Analysis ◽  
Specific Marker ◽  
Root Extract ◽  
Mangrove Species ◽  
Mangrove Plant ◽  
Layer Chromatography

Objective: An attempt has been made to study phytoconstituents and High-Performance Thin Layer Chromatography (HPTLC) fingerprinting pattern of leaf/stem/root methanol extracts of Avicennia marina.Methods: The phytochemical screening was done by standard biochemical methods and standard optimized HPTLC densitometry determination was performed under two different energy zones [under UV-254 nm and under 540 nm after derivatization at sample size-10 µl, temperature = 25.8±0.3 °C (constant) and relative humidity = 86±1% (constant) in optimized solvent system].Results: Phytochemical analysis confirmed the presence of phytochemicals in the leaf, stem, and root of the plant. Under UV–254 nm energy range, the leaf, stem and root extract showed the presence of 8, 6 and 5 components respectively and under 540 nm energy zone after derivatization, the leaf, stem and root extract revealed the presence of 7, 5 and 5 components, respectively and specific marker components with very high concentration (under UV-254 nm; 3, 2, 2 in leaf, stem, and root respectively and under 540 nm after ASR treatment; 2, 1, 2 in leaf, stem, and root respectively) were detected among them.Conclusion: The preliminary phytochemical test results elucidated Avicennia marina leaf, stem, and root as a hug reservoir of various class of phytochemicals and metabolites. The results obtained by HPTLC fingerprinting method found to be acceptable as a quick, reliable, accurate and economical for identification and authentication of Avicennia marina mangrove plant and useful to differentiate this mangrove species from other similar mangroves, in a single TLC plate run. This serves as a biochemical marker pattern for leaf, stem and root parts of this mangrove and for its diverse phytoconstituents.

scholarly journals COMPARATIVE STUDY ON HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY PROFILE AND ANTIMICROBIAL ACTIVITY OF ETHANOLIC AND HYDROALCOHOLIC EXTRACT OF VETIVERIA ZIZANIOIDES L. ROOT

2017 ◽  
Vol 10 (6) ◽  
pp. 336 ◽  
Author(s):  
Saravana Kumar Sivagurunathan ◽  
Gayathri Krishnamoorthy
Keyword(s):  
Antimicrobial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Ethanolic Extract ◽  
Vetiveria Zizanioides ◽  
Hydroalcoholic Extract ◽  
Significant Difference ◽  
Layer Chromatography

Objective: Scientific evaluation of traditionally using medicinal herbs for their pharmacological activity is a leading and valuable area of research. The aim of this study is to compare the antimicrobial activity of ethanolic and hydroalcoholic extract of Vetiveria zizanioides root and analyze the major bioactive compounds present in those extracts. Methods: Antimicrobial activity of both ethanolic and hydroalcoholic extracts was carried out against various pathogens such as Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Pseudomonas aeruginosa, and Candida albicans. A number of active compounds present in both extracts were compared by developing different compounds of the sample in high-performance thin layer chromatography (HPTLC) stationary phase using mobile phase petroleum ether:ethyl acetate:toluene:formic acid (5:5:1:1). Results: Ethanolic extract acts against pathogens such as S. aureus and MRSA, significantly (p<0.05) potent than that of hydroalcoholic extract. Significant difference has not been observed between ethanolic and hydroalcoholic extract when acts against P. aeruginosa and C. albicans. HPTLC profile of hydroalcoholic and ethanolic extract shows the presence of 10 and 14 different compounds, respectively, when developed with the same mobile phase. Gallic acid, a phenolic compound, was found to be present with higher % peak area in hydroalcoholic extract (3.25%) against ethanolic extract (2.98%). Conclusion: The results of this study reveal that zone of inhibition exhibited by both ethanolic and hydroalcoholic extracts was found to be different with dissimilar pathogens. A more number of compounds were eluted from hydroalcoholic extract than ethanolic extract. 

scholarly journals The Phytochemical Analysis of Hay Infusions and Papaya Leaf Juice as an Attractant Containing Insecticide for Aedes Aegypti

2017 ◽  
Vol 12 (2) ◽  
pp. 218-224
Author(s):  
Widya Hary Cahyati ◽  
Widya Asmara ◽  
S Rahmah Umniyati ◽  
Budi Mulyaningsih
Keyword(s):  
Lactic Acid ◽  
Aedes Aegypti ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chemical Compounds ◽  
Hplc Method ◽  
Phytochemical Analysis ◽  
Layer Chromatography ◽  
Leaf Juice

Aedes aegypti mosquito population could be controlled by using lethal ovitrap. The addition of hay infusions as a attractant greatly enhance Aedes aegypti eggs trapped, and papaya leaf juice may inhibit Aedes aegypti eggs evolve to larvae or a larvae to pupae stage. This study was conducted to find out the chemical compounds in hay infusion and papaya leaf juice. We used phytochemical test using UV-Vis Spectrophotometry, Thin Layer Chromatography, and High Performance Liquid Chromatoraphy (HPLC) method. The results showed that hay infusion contains 12,75 mg/L ammonium and <1,20 ppm (g/mL) lactic acid and papaya leaf juice contains 0,25% alkaloid, 0,14% flavonoid, 0,30% saponin, ?68 mg/L steroid and 11,34% tannin, but negative terpenoid. We concluded that hay infusion and papaya leaf juice contains chemical compounds that could be use as attractant and bioinsecticide to Aedes aegypti, respectively.

Sign in / Sign up

Export Citation Format

Share Document