RADIOIMMUNOASSAYS EMPLOYING DOUBLE ANTIBODY TECHNIQUES

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S247-S256 ◽  
Author(s):  
A. Rees Midgley ◽  
Robert W. Rebar ◽  
Gordon D. Niswender
Keyword(s):  
Solid Matrix ◽  
Soluble Antigen ◽  
Significant Extent ◽  
Reaction Conditions ◽  
The Third ◽  
Double Antibody ◽  
Specific Factors ◽  
Antigen Antibody ◽  
Ideal Method ◽  
Immunoprecipitation Reaction

ABSTRACT Double antibody methods are applicable to all radioimmunoassay systems. At least three approaches exist. The first, in which immunoprecipitating serum is used to precipitate soluble antigen-antibody complexes, is the most commonly used. Since the effects of interfering factors, especially those present in serum, become minimal as equilibrium is approached, care must be taken to insure that the period of incubation is of sufficient length for the immunoprecipitation reaction to reach equilibrium. In general, all efforts should focus on achieving reaction conditions which maximize stability and optimize immunoprecipitation. A second approach involves use of the first antiserum in an insolubilized form after prior precipitation with the immunoprecipitating antiserum. This approach is attractive since most non specific factors cannot interfere with the immunoprecipitation step to a significant extent once the formation of antigen-antibody aggregates is well underway. The third approach, in which immunoprecipitating antiserum is conjugated to a solid matrix, in principle represents a more ideal method of separating bound hormone from free. If this approach is ever to be utilized, a method of conjugating antiserum to a suitable matrix such that the antibody retains most of its immunoreactivity must be devised.

scholarly journals IMMUNOLOGICAL INDUCTION OF INCREASED VASCULAR PERMEABILITY

1969 ◽  
Vol 129 (1) ◽  
pp. 167-184 ◽  
Author(s):  
P. M. Henson ◽  
C. G. Cochrane
Keyword(s):  
Complement System ◽  
Soluble Antigen ◽  
Soluble Factor ◽  
Release Process ◽  
The Third ◽  
Rabbit Platelets ◽  
Antigen Antibody ◽  
Antibody Preparations ◽  
The Complement System

Two mechanisms have been described whereby rabbit platelets in vitro may be induced to release their contained histamine by the reaction of antigen and antibody. Both processes require the participation of the complement system. In the first, the adherence of platelets to particulate antigens such as zymosan or erythrocytes which have fixed complement through the third component was followed by histamine release. Plasma lacking C6 activity was fully active in this system. In the second mechanism, the reaction of soluble antigen with antibody in the presence of plasma also caused release of histamine from platelets and platelet clumping was observed. The release process, which appeared to follow the adherence of platelets to the immune complex, required the action of C6 and perhaps the later-acting components. No evidence could be obtained that a soluble factor was produced which caused the release. Instead, an inhibitor of the release process was detected after incubation of antigen, antibody, and plasma. Antibody preparations capable of giving complement-dependent PCA reactions in rabbits were also shown to induce the release of histamine from platelets in vitro.

Localization of Intracellular Antigens in thin Sections with Ferritin Labeled Antibody

1970 ◽  
Vol 28 ◽  
pp. 174-175
Author(s):  
M.S. Shahrabadi ◽  
T. Yamamoto
Keyword(s):  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Antigenic Determinants ◽  
Conjugate Prior ◽  
Thin Sections ◽  
Specific Attachment ◽  
Intracellular Antigen ◽  
Antigen Antibody ◽  
Ideal Method ◽  
The Ideal

The technique of labeling of macromolecules with ferritin conjugated antibody has been successfully used for extracellular antigen by means of staining the specimen with conjugate prior to fixation and embedding. However, the ideal method to determine the location of intracellular antigen would be to do the antigen-antibody reaction in thin sections. This technique contains inherent problems such as the destruction of antigenic determinants during fixation or embedding and the non-specific attachment of conjugate to the embedding media. Certain embedding media such as polyampholytes (2) or cross-linked bovine serum albumin (3) have been introduced to overcome some of these problems.

INHIBITION OF LIPOLYTIC ACTIVITY OF SYNTHETIC β1–24 AND β1–39 CORTICOTROPHIN BY ANTI-ACTH ANTISERUM

1966 ◽  
Vol 51 (1) ◽  
pp. 88-94 ◽  
Author(s):  
A. Villanueva ◽  
S. J. H. Ashcroft ◽  
J. P. Felber
Keyword(s):  
Guinea Pig ◽  
Lipolytic Activity ◽  
Peptide Hormones ◽  
Fat Pad ◽  
Double Antibody ◽  
Epididymal Fat ◽  
Antibody Complex ◽  
Radio Immunoassay ◽  
Antigen Antibody

ABSTRACT The synthetic ACTH peptides β1–39 and β1–24 stimulated lipolysis as determined by the rat epididymal fat pad in vitro. The stimulating effect of these peptides was diminished by prior incubation of the peptides with antibodies produced by the guinea-pig against ACTH. The stimulating effect of these hormones was also diminished by the double antibody system used in the radio-immunoassay of ACTH and other peptide hormones, in which incubation with antiserum is followed by precipitation of the antigen-antibody complex by rabbit anti-guinea-pig-γ-globulin.

Influence of Constructive and Technological Parameters at Generated Spiral Parts with DLP 3D Printing Process

2019 ◽  
Vol 56 (4) ◽  
pp. 801-811
Author(s):  
Mircea Dorin Vasilescu
Keyword(s):  
3D Printing ◽  
Great Influence ◽  
Polymerization Process ◽  
Generation Process ◽  
Technological Parameters ◽  
Printing Process ◽  
The Third ◽  
Solid Models ◽  
Specific Factors ◽  
Printing Processes

This work are made for determine the possibility of generating the specific parts of a threaded assembly. If aspects of CAD generating specific elements was analysed over time in several works, the technological aspects of making components by printing processes 3D through optical polymerization process is less studied. Generating the threaded appeared as a necessity for the reconditioning technology or made components of the processing machines. To determine the technological aspects of 3D printing are arranged to achieve specific factors of the technological process, but also from the specific elements of a trapezoidal thread or spiral for translate granular material in supply process are determined experimentally. In the first part analyses the constructive generation process of a spiral element. In the second part are identified the specific aspects that can generation influence on the process of realization by 3D DLP printing of the two studied elements. The third part is affected to printing and determining the dimensions of the analysed components. We will determine the specific value that can influence the process of making them in rapport with printing process. The last part is affected by the conclusions. It can be noticed that both the orientation and the precision of generating solid models have a great influence on the made parts.

Epistemic self-esteem of philosophers in the face of philosophical disagreement

Human Affairs ◽  
2020 ◽  
Vol 30 (3) ◽  
pp. 328-342
Author(s):  
László Bernáth ◽  
János Tőzsér
Keyword(s):  
Self Esteem ◽  
Moderate Level ◽  
Significant Extent ◽  
Fourth Section ◽  
Philosophical Beliefs ◽  
Low Level ◽  
The Third ◽  
Epistemic Peers ◽  
The Face ◽  
And Performance

AbstractOur paper consists of four parts. In the first part, we describe the challenge of the pervasive and permanent philosophical disagreement over philosophers’ epistemic self-esteem. In the second part, we investigate the attitude of philosophers who have high epistemic self-esteem even in the face of philosophical disagreement and who believe they have well-grounded philosophical knowledge. In the third section, we focus on the attitude of philosophers who maintain a moderate level of epistemic self-esteem because they do not attribute substantive philosophical knowledge to themselves but still believe that they have epistemic right to defend substantive philosophical beliefs. In the fourth section, we analyse the attitude of philosophers who have a low level of epistemic self-esteem in relation to substantive philosophical beliefs and make no attempt to defend those beliefs. We argue that when faced with philosophical disagreement philosophers either have to deny that the dissenting philosophers are their epistemic peers or have to admit that doing philosophy is less meaningful than it seemed before. In this second case, philosophical activity and performance should not contribute to the philosophers’ overall epistemic self-esteem to any significant extent.

57Fe Mössbauer Spectroscopic Study of the Geometrical Effects In the Catalytic Reactions with Intercalation Compounds

1982 ◽  
Vol 20 ◽  
Author(s):  
P.P. Vaishnava ◽  
P.A. Montano
Keyword(s):  
Ferric Chloride ◽  
Catalytic Reactions ◽  
Fischer Tropsch ◽  
Reaction Conditions ◽  
Intercalated Graphite ◽  
The Third ◽  
Intercalated Compounds ◽  
Catalytic Sites ◽  
Geometrical Effects

ABSTRACTIn situ 57Fe Mössbauer spectra are reported for the first-, higher-stage ferric chloride, and a mixed ferric chloride-potassium chloride intercalated graphite catalysts under reduction and Fischer-Tropsch reaction conditions. The mass spectroscopic measurements reveal a different catalytic selectivity for the three catalysts. The first two catalysts predominantly possess a higher selectivity for methane, whereas the third catalyst has higher selectivity for the formation of propane. The differences are attributed to geometrical effects in the catalytic sites of the intercalated compounds.

Vertical Stratification

2018 ◽  
pp. 125-150 ◽  
Author(s):  
Brendan Cantwell ◽  
Simon Marginson
Keyword(s):  
Higher Education ◽  
Middle Layer ◽  
Social Value ◽  
Vertical Stratification ◽  
The Third ◽  
The Social ◽  
High Participation ◽  
Specific Factors ◽  
Bifurcation Process ◽  
Positional Value

This chapter considers national system stratification in high participation systems (HPS) of higher education. As demand for higher education increases, the social value of places within a system becomes more differentiated on a binary basis, between places offering exceptionally high positional value and others offering little value. Three prepositions about stratification are advanced. The first expands on the tendency to system bifurcation in HPS, with a small and elite ‘artisanal’ sector, mostly research-intensive universities, opposed to a larger and undistinguished ‘demand-absorbing’ sector. The second proposition identifies a set of drivers that push the bifurcation process. The third proposition recognizes that bifurcation is always incomplete and focuses on the contradictory dynamics of the ‘middle’ layer of higher education institutions in most HPS. Nationally specific factors that accentuate or limit stratification are identified.

scholarly journals Translating the Commune: Cultural Politics and the Historical Specificity of the Anarchist Text

2007 ◽  
Vol 7 (1) ◽  
pp. 47-76 ◽  
Author(s):  
Donald Bruce
Keyword(s):  
Discourse Analysis ◽  
Cultural Politics ◽  
English Translation ◽  
Political Context ◽  
Functional Identity ◽  
Semantic Ambiguity ◽  
The Third ◽  
Historical Specificity ◽  
Specific Factors

Abstract Translating the Commune: Cultural Politics and the Historical Specificity of the Anarachist Text — This essay deals with three interrelated matters: the first is the role of discourse analysis and the conscious theorization of discourse typologies in translation methodologies; the second is the absence of any complete English translation of Jules Vallès's autobiographical/historical trilogy, Jacques Vingtras, comprised of L'Enfant (1879), Le Bachelier (1881), and L'insurgé (1885); and the third is the analysis of specific discursive characteristics which establish the formal and functional identity of the Discourse of the Commune. Though widely published in popular and scholarly editions in France, Vallès's novels have not been included in the lycée corpus through an act of conscious cultural exclusion. This has contributed to the exclusion of Vallès abroad and to the absence of translations of the trilogy. In order to remedy this situation the translator must be aware of the specific socio-political context surrounding these novels as well as the particular formal characteristics which make up the discourse from which these texts emerge. Radical decentralisation, narrative fragmentation, multiple enunciative positions, neologisms, a structure based on an unresolved binary dialectic, interdiscursive mixing and semantic ambiguity are common characteristics of the discourse of the Commune as they are transposed metaphorically from the anarchistic theoretical discourse of P.-J. Proudhon to the Vallès texts: these specific factors coupled with a cultural politics of exclusion have long marginalized the trilogy in various curricula and, in addition, led to its exclusion from non-francophone cultures both in the original French and in translation.

scholarly journals THE PATHOLOGIC EFFECTS OF INTRAVENOUSLY ADMINISTERED SOLUBLE ANTIGEN-ANTIBODY COMPLEXES

1960 ◽  
Vol 111 (2) ◽  
pp. 195-200 ◽  
Author(s):  
Baruj Benacerraf ◽  
Jacobus L. Potter ◽  
Robert T. McCluskey ◽  
Frederick Miller
Keyword(s):  
Blood Urea Nitrogen ◽  
Intravenous Administration ◽  
Acute Glomerulonephritis ◽  
Soluble Antigen ◽  
Urea Nitrogen ◽  
Antigen Antibody

The intravenous administration to rats of soluble antigen-antibody complexes in antigen excess resulted in acute glomerulonephritis. This occurred with both rabbit antiovalbumin and rabbit anti-BSA systems. The rats so treated regularly showed proteinuria and elevation of blood urea nitrogen. These findings are compared with those reported in rats injected with anti-kidney serum.

Sign in / Sign up

Export Citation Format

Share Document