Total and non-dialyzable urinary hydroxyproline in acromegalics and control subjects

1981 ◽  
Vol 96 (4) ◽  
pp. 451-457 ◽  
Author(s):  
Johan Halse ◽  
Jan O. Gordeladze
Keyword(s):  
Urinary Excretion ◽  
Formation Rate ◽  
Bone Matrix ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Control Subjects ◽  
Urinary Parameters ◽  
Turn Over ◽  
And Control ◽  
Calcium Phosphate Metabolism

Abstract. The urinary excretion of total and non-dialyzable hydroxyproline (HYP) containing peptides has been studied in 25 patients with active acromegaly and 44 control subjects — all kept on a collagen free diet. Acromegalics had a greater excretion of both total and non-dialyzable HYP than controls, indicating an increased turn-over of collagen/bone matrix. The amounts of total and non-dialyzable HYP excreted in acromegalics were significantly correlated to mean fasting growth hormone levels. Both acromegalics and controls exhibited a significant coupling between the excretion of total and non-dialyzable HYP. Female control subjects demonstrated an age-dependent decrease in the excretion of both total and non-dialyzable HYP, whereas no such age-dependence could be observed in males. Age seemed to have a greater effect on the non-dialyzable fraction than on the total HYP excretion. These results may imply that collagen/bone matrix turn-over declines with age and that bone formation declines at a more rapid rate than resorption. In acromegalics the ratio of non-dialyzable to dialyzable HYP was not significantly different from control values, indicating that although turn-over is increased the actual matrix formation rate is normal in acromegaly. No correlation was found between serum or urinary parameters of calcium/phosphate metabolism or the urinary excretion of 3',5'-cyclic adenosine monophosphate and total or non-dialyzable HYP in acromegalics or controls.

scholarly journals Cyclic AMP response element-binding protein is required in excitatory neurons in the forebrain to sustain wakefulness

SLEEP ◽  
2020 ◽  
Author(s):  
Mathieu E Wimmer ◽  
Rosa Cui ◽  
Jennifer M Blackwell ◽  
Ted Abel
Keyword(s):  
Cyclic Amp ◽  
Intracellular Signaling ◽  
Target Genes ◽  
Binding Protein ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Response Element ◽  
Creb Phosphorylation ◽  
Element Binding Protein ◽  
And Control

Abstract The molecular and intracellular signaling processes that control sleep and wake states remain largely unknown. A consistent observation is that the cyclic adenosine monophosphate (AMP) response element-binding protein (CREB), an activity-dependent transcription factor, is differentially activated during sleep and wakefulness. CREB is phosphorylated by the cyclic AMP/protein kinase A (cAMP/PKA) signaling pathway as well as other kinases, and phosphorylated CREB promotes the transcription of target genes. Genetic studies in flies and mice suggest that CREB signaling influences sleep/wake states by promoting and stabilizing wakefulness. However, it remains unclear where in the brain CREB is required to drive wakefulness. In rats, CREB phosphorylation increases in the cerebral cortex during wakefulness and decreases during sleep, but it is not known if this change is functionally relevant to the maintenance of wakefulness. Here, we used the Cre/lox system to conditionally delete CREB in the forebrain (FB) and in the locus coeruleus (LC), two regions known to be important for the production of arousal and wakefulness. We used polysomnography to measure sleep/wake levels and sleep architecture in conditional CREB mutant mice and control littermates. We found that FB-specific deletion of CREB decreased wakefulness and increased non-rapid eye movement sleep. Mice lacking CREB in the FB were unable to sustain normal periods of wakefulness. On the other hand, deletion of CREB from LC neurons did not change sleep/wake levels or sleep/wake architecture. Taken together, these results suggest that CREB is required in neurons within the FB but not in the LC to promote and stabilize wakefulness.

Increased urinary excretion of prostaglandin E2 in patients with idiopathic hypercalciuria is a primary phenomenon

1992 ◽  
Vol 83 (1) ◽  
pp. 75-80 ◽  
Author(s):  
C. Henríquez-la Roche ◽  
B. Rodríguez-Iturbe ◽  
G. Parra
Keyword(s):  
Urinary Excretion ◽  
Prostaglandin E2 ◽  
Idiopathic Hypercalciuria ◽  
Water Restriction ◽  
Water Diuresis ◽  
Control Subjects ◽  
Calcium Diet ◽  
High Calcium ◽  
And Control ◽  
Primary Phenomenon

1. Urinary excretion of prostaglandin E2 is increased in patients with idiopathic hypercalciuria, but in order to conclude that hyperprostaglandinuria is a primary phenomenon, it must be demonstrated that high levels of urinary prostaglandin E2 can be dissociated from other factors, such as urine volume and natriuresis, and from the hypercalciuria itself. 2. We studied 10 patients with idiopathic hypercalciuria and 10 control subjects on high and low calcium diets providing daily calcium intakes of 30-35 mmol and 7.5-10 mmol, respectively, and similar sodium intakes. In addition, patients with idiopathic hypercalciuria and control subjects were studied during water restriction and water diuresis. 3. Urinary prostaglandin E2 excretion was more than twice as high in patients with idiopathic hypercalciuria than in control subjects on the low and high calcium diets as well as during water restriction and water diuresis (P<0.01). 4. Urinary prostaglandin E2 excretion was not affected by changes in urinary calcium excretion in patients with idiopathic hypercalciuria and in control subjects. Patients with idiopathic hypercalciuria on the low calcium diet and control subjects on the high calcium diet had similar levels of calciuria and natriuresis, yet urinary prostaglandin E2 excretion (mean ± SEM) was 11.62 ± 1.71 nmol/day in the patients with idiopathic hypercalciuria and 3.26 ± 0.48 nmol/day in the control subjects (P= 0.0006). 5. These results indicate that increased urinary prostaglandin E2 excretion is a cardinal characteristic of patients with idiopathic hypercalciuria.

URINARY EXCRETION OF CALCIUM, HYDROXYPROLINE AND 3′,5′-CYCLIC ADENOSINE MONOPHOSPHATE IN PRIMARY HYPERPARATHYROIDISM

1979 ◽  
Vol 92 (1) ◽  
pp. 138-147 ◽  
Author(s):  
Johan Halse ◽  
Jan O. Gordeladze
Keyword(s):  
Food Intake ◽  
Primary Hyperparathyroidism ◽  
Urinary Excretion ◽  
Diurnal Variation ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Parathyroid Surgery ◽  
Creatinine Ratio ◽  
Parathyroid Function ◽  
The Individual

ABSTRACT Urinary excretion of calcium (Ca), hydroxyproline (Hyp) and 3′,5′-cyclic adenosine monophosphate (cAMP) was measured during fasting, and in the afternoon, over a 3 day period. Twelve hyperparathyroid patients, of whom 6 were re-studied after successful parathyroid surgery, and 10 control subjects participated, and were maintained on a collagen free diet for the duration of the study. Expressed as creatinine ratio values, Hyp was significantly higher in the morning than during the afternoon, whereas the Ca excretion pattern showed low morning and high afternoon values for all groups. cAMP excretion did not change during the two sampling periods. Large day to day variations for each parameter were observed in the individual patient. The value of cAMP measurements in the diagnosis of primary hyperparathyroidism was confirmed. The results may imply that a diurnal variation in Hyp excretion exists in primary hyperparathyroidism and that food intake produces a suppression of Hyp excretion, possibly secondary to suppression of parathyroid function or, in our view, to increased calcitonin excretion.

scholarly journals Effect of Electric Convulsion Therapy on Urinary Excretion of 3', 5' Cyclic Adenosine Monophosphate

BMJ ◽  
1972 ◽  
Vol 3 (5824) ◽  
pp. 439-441 ◽  
Author(s):  
K. Hamadah ◽  
H. Holmes ◽  
G. B. Barker ◽  
G. C. Hartman ◽  
D. V. W. Parke
Keyword(s):  
Urinary Excretion ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate

Calcium and magnesium in essential hypertension

1988 ◽  
Vol 75 (4) ◽  
pp. 395-402 ◽  
Author(s):  
D. M. Tillman ◽  
P. F. Semple
Keyword(s):  
Essential Hypertension ◽  
Urinary Excretion ◽  
Systolic Pressure ◽  
Ionized Calcium ◽  
Serum Concentrations ◽  
Total Calcium ◽  
Control Subjects ◽  
Calcium Magnesium ◽  
The Difference ◽  
And Control

1. Because disturbances of calcium metabolism have been described in hypertension, measurements of plasma and serum concentrations of ionized calcium, total calcium, magnesium and renin were made in 38 patients with essential hypertension and age- and sex-matched control subjects. Urinary excretion of calcium, magnesium and sodium was also determined. 2. The mean serum concentration of ionized calcium was 1.23 ± 0.04 (sd) mmol/l in the hypertensive group and 1.21 ±0.03 mmol/l in controls, and results were similar after correction for pH. There was a weak positive correlation between serum ionized calcium (pH 7.4) and systolic pressure (r = 0.26, P < 0.02), but no correlation with plasma renin concentration. 3. Although the difference between serum total calcium concentration in the hypertensive (2.29 ±0.09 mmol/l) and control (2.26 ±0.07 mmol/l) subjects was not significant, there was a significant correlation between total calcium and systolic pressure (r = 0.23, P < 0.05) which was maintained after correction for other variables. 4. There were no differences in plasma concentrations of parathyroid hormone or 1,25-dihydroxycholecalciferol between hypertensive and control subjects. 5. The hypertensive group showed higher urinary excretion of calcium (5.9 ±3.0 mmol/24h) than controls (4.6 ± 1.7 mmol/24 h), but the difference was not maintained after correction for sodium excretion. 6. Serum concentrations of magnesium were similar in the two groups, but urinary excretion of magnesium was significantly lower in hypertensive (3.7 ± 1.3 mmol/24 h) than control (4.5 ±1.6 mmol/24 h) subjects and there was an inverse correlation between magnesium excretion and blood pressure (r = 0.3–0.35, P < 0.01).

46 VITRIFICATION AT THE GERMINAL VESICLE STAGE TRIGGERS PRECOCIOUS MEIOTIC RESUMPTION BUT DOES NOT AFFECT CYTOPLASMIC MATURATION IN CUMULUS-ENCLOSED PORCINE OOCYTES DURING IN VITRO MATURATION

2016 ◽  
Vol 28 (2) ◽  
pp. 153
Author(s):  
T. Somfai ◽  
N. T. Men ◽  
H. Kaneko ◽  
J. Noguchi ◽  
S. Haraguchi ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
In Vitro Maturation ◽  
Germinal Vesicle ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Control Group ◽  
Control Groups ◽  
Porcine Oocyte ◽  
And Control

Previously we have reported a vitrification protocol that allows preservation of immature porcine oocytes in large numbers (Somfai et al. 2014 PLoS One 9, e97731). However, despite high survival rates, embryo development rates have remained low. The aim of our current research is to reveal factors potentially responsible for reduced developmental competence of vitrified oocytes. As a first step, we investigated the effects of vitrification at the germinal vesicle stage on subsequent nuclear progression and the normality of cytoplasmic functions during in vitro maturation (IVM). Cumulus-enclosed porcine oocytes were vitrified in microdrops, stored, and then warmed by our method (Somfai et al. 2015 Reprod. Fertil. Dev. 27, 124). Then the oocytes were subjected to IVM for 46 h in a chemically defined porcine oocyte medium. During the first 22 h of IVM, the medium was supplemented with 1 mM dibutyryl cyclic adenosine monophosphate, 10 IU mL–1 of eCG, and 10 IU mL–1 of hCG. The following 24 h of IVM was performed in porcine oocyte medium without any supplementation. We compared vitrified/warmed oocytes (vitrified group) with freshly collected immature oocytes (control group) in terms of (1) nuclear progression, (2) intracellular glutathione (GSH), and (3) adenosine triphosphate levels throughout IVM. Each experiment was replicated at least 3 times. Results were analysed by one-way ANOVA and Tukey’s multiple comparison test. A total of 510 oocytes were vitrified of which 422 (82.3%) survived. Only live oocytes were subjected to subsequent assays. Orcein staining revealed that after 22 h of IVM, a significantly higher percentage (P < 0.05) of vitrified oocytes showed germinal vesicle breakdown compared with the control group (22.0 v. 0.9%, respectively). In a similar fashion, after 30 h IVM, a significantly higher (P < 0.05) percentage of oocytes reached the metaphase-II (MII) stage in the vitrified group than in the control group (21.8 v. 0%, respectively). After 46 h of IVM, there was no difference between the vitrified and control groups in terms of the percentage of MII stage oocytes (93.9 and 86.3%, respectively). Analysis of GSH levels in oocytes by the 5,5′-dithio-bis-2-nitrobenzoic acid-glutathione disulfide reductase recycling assay showed no significant difference between the vitrified and control groups at 0 h (6.7 and 7.0 pmol, respectively), 22 h (5.5 and 5.5 pmol, respectively), and 46 h (6.9 and 7.9 pmol, respectively) of IVM. Adenosine triphosphate assay (FL-ASC; Sigma-Aldrich Co., St. Louis, MO) revealed similar adenosine triphosphate contents in the oocytes of the vitrified and control groups at 0 h (1.53 and 1.61 pmol, respectively), 22 h (1.67 and 1.70 pmol, respectively), and 46 h (1.65 and 1.83 pmol, respectively) of IVM. In conclusion, vitrification triggered precocious nuclear maturation even in the presence of dibutyryl cyclic adenosine monophosphate; however, it did not affect GSH levels and overall metabolism. This work was supported by JSPS KAKENHI (Grant Number: 26870839) and JST/JICA SATREPS.

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