Effects of seven different oestrogen sulphates on uterine growth and on progesterone receptor in the foetal uterus of guinea pig after administration to the mother

1982 ◽  
Vol 101 (4) ◽  
pp. 630-635 ◽  
Author(s):  
J. R. Pasqualini ◽  
A. Lanzone ◽  
A. Tahri-Joutei ◽  
B. L. Nguyen
Keyword(s):  
Progesterone Receptor ◽  
Guinea Pig ◽  
Specific Binding ◽  
Biological Effects ◽  
Biological Response ◽  
The Other ◽  
Specific Binding Sites ◽  
Other Hand ◽  
Uterine Growth ◽  
Two Parameters

Abstract. The biological effect of seven different oestrogen sulphates: oestrone-3-sulphate (E1-3-S), oestradiol-3-sulphate (E2-3-S), oestradiol-17-sulphate (E2-17-S), oestradiol-3,17-disulphate (E23,17-DS), oestriol-3-sulphate (E3-3-S), oestriol-17-sulphate (E3-17-S), oestriol-3,16,17-trisulphate (E3-3,16,17-TS), was studied in the foetal uterus of guinea pig (55–65 days of gestation) after sc administration for 3 consecutive days of each sulphate to the mother. On day 4, two response parameters were investigated, the uterotrophic effect and the action on the progesterone receptor. The monosulphates at the C3 position of the oestrogens (E1-3-S, E2-3-S and E3-3-S) provoked an increase in uterine weight of 1.9–2.4 times in relation to the non-treated animals. These oestrogen sulphates also very significantly stimulated the number of progesterone specific binding sites, 7–10 times in relation to the non-treated animals. On the other hand, when the sulphate was at C17 of the oestrogens (E2-17-S, E3-17-S, E2-3, 17-DS, E3-3,16,17-TS), very little or no effect on the foetal uterus was observed on the two parameters studied. It is concluded that oestrogen (oestrone, oestradiol, oestriol) sulphates in position C3 can be involved in the biological response to the hormone and it is suggested that the effect is carried out after the hydrolysis of the sulphate. On the other hand, sulphates in C17 are not hydrolysed and no significant biological effects were observed in the uterine growth or in the progesterone receptor.

Biological effects and ultrastructural alterations of cis-tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen in the uterus and vagina of newborn guinea pigs

1988 ◽  
Vol 119 (1) ◽  
pp. 85-90 ◽  
Author(s):  
F. Lecerf ◽  
B.-L. Nguyen ◽  
J. R. Pasqualini
Keyword(s):  
Progesterone Receptor ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Biological Effects ◽  
Progesterone Receptors ◽  
The Other ◽  
Short Treatment ◽  
Morphological Alterations ◽  
Ultrastructural Alterations ◽  
Other Hand

Abstract. The biological and morphological effects of cis-tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen, administered sc alone (100 μg/animal) or combined with estradiol (20 μg/animal) were studied in the uterus and vagina of the guinea pig. After 2 days treatment the values of the uterine wet weights (mg ± sd of 6–10 animals in each study) were as follows: non-treated animals (control): 142 ± 15; animals treated with cis-tamoxifen: 119 ± 4; N-desmethyltamoxifen: 280 ± 20; 4-hydroxytamoxifen: 268 ± 25. The values after long treatment were: 177 ± 30; 490 ± 65; 394 ± 36 and 581 ± 60, respectively. After short treatment the weights of the vaginas were: control: 99 ± 20; cis-Tamoxifen: 67 ± 2; N-desmethyltamoxifen: 153 ± 25; 4-hydroxytamoxifen: 166 ± 7; and after the long treatment: 155 ± 40; 660 ± 41; 467 ± 38 and 502 ±61, respectively. N-desmethyltamoxifen and 4-hydroxytamoxifen increased the progesterone receptors in the uterus after short treatment (P < 0.01) but not after 12 days treatment. On the other hand, there was no effect on progesterone receptor in the vagina after the short treatment but a very stimulatory effect after the long treatment. The morphological alterations after 12-days treatment indicate that the three tamoxifen derivatives in the two tissues studied provoke intense alterations in different organelles. In conclusion, it is suggested that the tamoxifen derivatives can act as real agonists in the uterus and vagina of the newborn guinea pig, and they do not block the effect provoked by estradiol.

Comparative response to oestradiol in the synthesis of the progesterone receptor in the maternal and foetal uterus of guinea pig and rabbit at the end of gestation

1981 ◽  
Vol 98 (1) ◽  
pp. 126-132 ◽  
Author(s):  
Francoise Laure ◽  
Jorge Raul Pasqualini
Keyword(s):  
Progesterone Receptor ◽  
Guinea Pig ◽  
Binding Sites ◽  
Saline Solution ◽  
Specific Binding ◽  
Cytosol Fraction ◽  
Specific Binding Sites ◽  
Comparative Response ◽  
Injection Control ◽  
Stimulation Of

Abstract. In the present paper the stimulatory effect of oestradiol on the progesterone receptor (PR) in two species: rabbit and guinea pig, is studied in both the maternal and foetal uterine tissues. Oestradiol (1 mg/kg/day) in saline solution was injected sc into the maternal compartment of pregnant guinea pig and rabbit at the end of gestation for 3 consecutive days and the PR evaluated 24 h after the last injection; control animals received the vehicle alone. In the maternal uterus the number of specific binding sites in the cytosol fraction for the synthetic progestagen R-5020 (17α,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione) in the non-treated animals was, for the rabbit 117 ± 25 (sd) fmol/mg protein and for the guinea pig 325 ± 62 (sd) fmol/mg protein. In the treated animals a stimulation is observed of 2.5–3 times, 315 ± 85 (sd) fmol/mg protein for the maternal uterus of rabbit and 865 ± 100 (sd) fmol/mg protein for the maternal uterus of guinea pig. In the foetal uterus it is a very different picture, while in the foetal uterus of guinea pig a great stimulatory effect is observed on the PR; from 267 ± 53 (sd) fmol/mg protein in the nontreated animals to 1984 ± 357 (sd) in the oestradiolprimed animals; in the foetal uterus of rabbit very little or no PR is detectable in the non-treated animals while in the treated animals with the same or 1/20 of the dose of oestradiol for only 1 day, the death of the foetus was provoked. In summary, the effect of oestradiol on the stimulation of PR in maternal uterine tissue is similar in these two species. In the foetal uterus of guinea pig the response to the action of oestradiol on the PR is very significant but this hormone is of great toxicity for the rabbit foetus. It is concluded that the oestradiol response of the foetus is related to the maturity of the foetus.

scholarly journals A SOLUBLE ANTIGEN OF LYMPHOCYTIC CHORIOMENINGITIS

1940 ◽  
Vol 72 (4) ◽  
pp. 389-405 ◽  
Author(s):  
J. E. Smadel ◽  
M. J. Wall
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Lymphocytic Choriomeningitis ◽  
The Other ◽  
Soluble Antigen ◽  
Soluble Substance ◽  
Other Hand ◽  
The Times

Anti-soluble substance antibodies and neutralizing substances, which develop following infection with the virus of lymphocytic choriomeningitis, appear to be separate entities. The times of appearance and regression of the two antibodies are different in both man and the guinea pig; the antisoluble substance antibodies appear earlier and remain a shorter time. Moreover, mice develop them but no demonstrable neutralizing substances. Injection of formalin-treated, virus-free extracts containing considerable amounts of soluble antigen fails to elicit anti-soluble substance antibodies and to induce immunity in normal guinea pigs; administration of such preparations to immune pigs, however, is followed by a marked increase in the titer of anti-soluble substance antibodies in their serum. On the other hand, suspensions of formolized washed virus are effective in normal guinea pigs in stimulating both anti-soluble substance antibodies and protective substances, and in inducing immunity to infection.

Effect of heavy metals and lanthanum on sugar transport in isolated guinea pig left atria

1980 ◽  
Vol 58 (10) ◽  
pp. 1184-1188 ◽  
Author(s):  
I. Bihler ◽  
L. E. Hoeschen ◽  
P. C. Sawh
Keyword(s):  
Heavy Metals ◽  
Guinea Pig ◽  
Binding Sites ◽  
Specific Binding ◽  
Sugar Transport ◽  
Free Medium ◽  
Specific Binding Sites ◽  
Stimulation Of

The effect of heavy metals on sugar transport in fully resting guinea pig left atria was studied by measuring the tissue–medium distribution of 3-methylglucose. Basal sugar transport was increased significantly by all heavy metals tested (Co2+, Ni2+, Zn2+, Mn2+ (2 mM)) and by La3+ (0.05 mM) but 1 mM La3+ had no effect. The stimulation of sugar transport by insulin, hyperosmolarity, K+-free medium, or 10−5 M ouabain was strongly antagonized by Ni2+, Zn2+, and La3+ but was unaffected by Co2+ and Mn2+. The heavy metals did not affect intracellular Na2+ and K+, whether in the basal state or when the Na+ pump was depressed by ouabain or K+-free medium. The data suggest that Ca2+ antagonistic ions may affect sugar transport both by inhibiting Ca2+ influx and by competing with Ca2+ for specific binding sites presumably involved in the regulation of sugar transport.

scholarly journals EFFECTS OF MECHANICAL AGITATION AND OF TEMPERATURE UPON COMPLEMENT

1911 ◽  
Vol 13 (2) ◽  
pp. 229-233 ◽  
Author(s):  
Hideyo Noguchi ◽  
J. Bronfenbrenner
Keyword(s):  
Guinea Pig ◽  
The Other ◽  
Mechanical Agitation ◽  
Destructive Effect ◽  
Complementary Activity ◽  
Other Hand ◽  
Original Strength ◽  
Pig Serum

1. Under certain conditions, mechanical agitation destroys the complementary activity of guinea pig serum. It is most injurious when carried out constantly at a temperature of 37° C., but it is extremely insignificant at 10° C. After the first few hours at 37° C., the destruction of complement proceeded much more rapidly, and after six hours it was almost complete. On the other hand, within one hour shaking had almost no destructive effect on complement, even at 37° C. From this we may conclude that the several shakings which are necessary for fixation experiments during incubation do not modify perceptibly the outcome of the reactions. 2. The rate of destruction of the complement of guinea pig serum at temperatures above 45° C. is progressively greater as it approaches 55° C., at which temperature the activity is reduced in thirty minutes to one-thirtieth to one-fortieth of the original strength of the unheated serum; but it is not completely destroyed, as is commonly assumed. The velocity of destruction of guinea pig complement when exposed to 55° C. for various lengths of time is found to be quite irregular, and not proportional to the length of time. This irregularity, however, presents a certain rhythm, a period of greater destruction alternating with one of less destruction.

scholarly journals El género narrativo de Testo yonqui. Aproximación a la deriva ensayística de Paul B. Preciado

2020 ◽  
pp. 191-214
Author(s):  
Pau Conde Arroyo
Keyword(s):  
Guinea Pig ◽  
Theoretical Framework ◽  
The Self ◽  
The Other ◽  
Other Hand ◽  
The One ◽  
Definition Of ◽  
The Relationship

Este artículo trata de problematizar la definición taxonómica de Testo yonqui desde una óptica literaria que atiende a su faceta narrativa para dilucidar los cauces por los que se manifiesta en tanto que ensayo queer. Dicha problematización es abordada desde dos lugares: por un lado, desde la propia obra, atendiendo a las autodefiniciones presentes en el texto, que son examinadas a partir del marco teórico de la autobiografía; y, por otro lado, desde la recepción crítica de Testo yonqui. En último lugar, a la luz de lo anterior, se exponen una serie de tensiones relativas a la relación entre narración, referente y representación en la propuesta experimental del principio autocobaya.   This article aims to question the taxonomical definition of Testo Junkie from a literary perspective that considers its narrative aspect in order to elucidate the ways in which it can be regarded as a queer essay. Such questioning is approached from two angles: on the one hand, from the work itself, examining the self-definitions found in the text, which are studied on the basis of the theoretical framework of autobiography; and, on the other hand, from Testo Junkie’s critic reception. Lastly, the principle of the auto-guinea pig is also explored, in reference to the series of tensions arising from the relationship between narration, referent and representation.

scholarly journals Clitic or Agreement Restriction in Santali: A Typological Analysis

2020 ◽  
Vol 10 (1) ◽  
pp. 9-33
Author(s):  
Mayuri J. DILIP ◽  
Rajesh KUMAR
Keyword(s):  
The Other ◽  
Nominative Case ◽  
Number Marking ◽  
Other Hand ◽  
Subject And Object ◽  
Subject Agreement ◽  
Object Agreement ◽  
Two Parameters ◽  
Feature Checking

This paper investigates the syntactic configuration of pronominal number marking in Santali. Syntactic, morphological and prosodic restrictions show that pronominal number markers have properties of an affix as well as a clitic. A marker is an affix due to the fact that it cannot participate in a binding relation with other arguments. A pronominal number marker also functions as a clitic since it is attached to prosodically the most prominent constituent. The arguments that trigger object agreement do not manifest one particular case, but instead indicate a dissociation between a case and object agreement. On the other hand, the argument with subject agreement manifests nominative case only, indicating an association between nominative case and subject agreement. Both subject and object agreement are sensitive to case that indicates a property of an affix. Keeping in view the distribution of the pronominal number markers, we analyze feature checking of the two parameters, namely agreement and case in Santali.

Chemoselective cluster labelling of biomolecules

1987 ◽  
Vol 45 ◽  
pp. 760-761
Author(s):  
C. J. Foley ◽  
L. E. Maelia ◽  
J. F. Hainfeld ◽  
J. S. Wall
Keyword(s):  
Binding Sites ◽  
Specific Binding ◽  
Heavy Atom ◽  
The Other ◽  
Biological Structure ◽  
Heavy Atoms ◽  
Specific Binding Sites ◽  
Lower Beam ◽  
High Concentrations ◽  
Beam Dose

The Brookhaven STEM is capable of visualizing single heavy atoms at a beam dose of >103 el/Å2. Heteropolytungstate clusters, including W12PO403, have been found to incorporate several desirable properties as labels for biological specimens. They may be resolved at much lower beam doses due to their high concentrations of multiple heavy atoms and are directly visible labels. A lower beam dose also helps to preserve the biological structure of the specimens. Furthermore, they are extremely stable in the electron beam. Lastly, they are capable of being derivatized as chemoselective reagents for specific binding sites on biomolecules, as in the previously reported undecagold compound.Two new classes of heavy atom labels, one specific for sulfhydryl and the other specific for both amino and sulfhydryl binding sites on proteins, have been synthesized by reactions analogous to those illustrated in Scheme 1.

Contribution of technology to human cell properties

2021 ◽  
pp. 1-4
Author(s):  
Carlota Saldanha
Keyword(s):  
Endothelial Cell ◽  
Human Cell ◽  
Binding Sites ◽  
Blood Cells ◽  
Specific Binding ◽  
Vascular Endothelial Cell ◽  
The Other ◽  
Vascular Endothelial ◽  
Specific Binding Sites ◽  
Human Lungs

After explaining the meaning of SARS-CoV2, the protection rules for the disease caused by this virus are described in order to eradicate the resulting pandemic. Methods to differentiate asymptomatic from symptomatic patients will be mentioned. Human lungs, heart, kidney, endothelium and erythrocyte have specific binding sites for the SARS-CoV2. The aim of this opinion was to highlight some new disposable technology to identify two cell properties. One of them is the vascular endothelial cell (EC) receptor binding to the SARS-CoV2 and the other is related with red blood cells (RBCs) as SARS-CoV2 carrier.

Chronicle

1934 ◽  
Vol 30 (2) ◽  
pp. 224-227
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
The Other ◽  
Subsequent Infection ◽  
Other Hand

The All-Ukrainian Bacteriological Institute is developing with successful results the question of protective vaccinations against typhus. A number of experiments performed on guinea pigs showed that guinea pig infected with typhus passerine virus and having suffered the disease is immune to subsequent infection with the blood of a typhus-typhoid patient. On the other hand, guinea pig infected with the blood of a typhoid patient and having survived the disease appears immune to infection with the guinea pig passage virus.

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