Appraisal of endocrine function of segmental autotransplanted pancreas in dogs

1984 ◽  
Vol 105 (1) ◽  
pp. 72-77 ◽  
Author(s):  
M. Jonung ◽  
Y. Berlatzky ◽  
M.-H. Chen ◽  
R. Munda ◽  
I. G. Banks ◽  
...  
Keyword(s):  
Secretory Granules ◽  
Iliac Fossa ◽  
Electron Microscopic Examination ◽  
Pancreatic Tissue ◽  
Experimental Models ◽  
Endocrine Function ◽  
Electron Microscopic ◽  
Glucose Levels ◽  
Patients With Diabetes ◽  
Glucagon Immunoreactivity

Abstract. There is increasing interest in pancreatic transplantation for patients with diabetes. In experimental models, endocrine function is usually monitored by determination of insulin and glucose levels in plasma. In this study following a segmental pancreatic autotransplant to the iliac fossa in dogs, a combined analysis of three pancreatic islet hormones, insulin, pancreatic polypeptide (PP) and glucagon was undertaken by radioimmunoassay of plasma. These were measured under basal conditions and following provocation with a standard meal, arginine, secretin and bombesin infusions. Immunohistochemical and electron microscopic examination of transplanted tissue was also performed. Circulating insulin and glucose levels in the surviving dogs with transplants reflected normoglycaemia with a normal tolerance to iv glucose and immunohistochemical detection of endocrine cells producing insulin, PP and glucagon. Secretory granules were found in A and B cells by electron microscopy. The normal circulating glucagon immunoreactivity could have originated in gastric antral A cells as well as in pancreatic tissue. It was not possible, however, to stimulate the autotransplanted pancreas to release detectable PP into the circulation.

Multiple supratentorial hemangioblastomas

1971 ◽  
Vol 35 (4) ◽  
pp. 396-405 ◽  
Author(s):  
Shakuntla Ishwar ◽  
Raymond M. Taniguchi ◽  
F. Stephen Vogel
Keyword(s):  
Secretory Granules ◽  
Electron Microscopic Examination ◽  
Extramedullary Hematopoiesis ◽  
Electron Microscopic ◽  
Content Type ◽  
Parietal Area ◽  
Erythropoietin Production ◽  
Well Differentiated ◽  
The Right ◽  
Mass Lesions

✓ Multiple hemangioblastomas were found above the tentorium in a 62-year-old woman who first had a discrete 2.5 cm tumor removed from the superior aspect of the tentorium adjacent to the falx, and 2 years later developed two more intracranial mass lesions, one in the right parietal area, the other attached to the dura of the right frontal fossa. Histological examination of all three tumors showed precisely the same well-differentiated morphology of hemangioblastoma. Foci of extramedullary hematopoiesis were present within them. Electron microscopic examination of the last two lesions disclosed secretory granules within the tumor cells, indicative of erythropoietin production, as described previously in a hemangioblastoma. Collagen and endothelial cells were not present in quantities consistent with an angioblastic meningioma. The genesis of hemangioblastomas is discussed.

scholarly journals Using of the method of electron microscopy for the identification and analysis of clinically implicit pathogenetic manifestations

2018 ◽  
Vol 7 (3) ◽  
pp. 113-116
Author(s):  
L. G. Nikonova ◽  
V. V. Banin ◽  
I. G. Stel'nikova
Keyword(s):  
Diabetes Mellitus ◽  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
B Cells ◽  
Secretory Granules ◽  
Electron Microscopic Examination ◽  
The Body ◽  
Electron Microscopic ◽  
Latent Form ◽  
Insulin Cells

Electron microscopic examination of B cells of pancreatic islets of the pancreas in dogs with normal (n=10) and impaired glucose tolerance (n=10) was performed. Ultrastructural features of the organization of insulin cells associated with an increased requirement of the hormone in the body with the latent form of diabetes mellitus are established. In B cells, signs of functional tension due to unregulated secretion, manifested by the expansion of endoplasmic reticulum cisterns, Golgi complex hypertrophy, an increase in the number of immature secretory granules and vacuoles in the cytoplasm are revealed in B cells.

Effect of Streptozotocin-Induced Diabetes on Estradiol-Stimulated Changes in the Ultrastructure of the Rat Endometrium

1981 ◽  
Vol 39 ◽  
pp. 532-533
Author(s):  
G.T. Frederick ◽  
R.M. Gardner ◽  
J.M. Kirkland ◽  
G.M. Stancel
Keyword(s):  
Electron Microscopic Examination ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Whole Cells ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sprague Dawley Rats ◽  
Uterine Growth ◽  
Streptozotocin Induced Diabetes

Estradiol (E2)-stimulated uterine growth has been well characterized both biochemically and morphologically. Recent studies have shown that insulin plays an important role in the regulation of cellular development. This study examines the effect of streptozotocin-induced diabetes on E2-stimulated changes in the ultrastructure of the endometrium of the rat uterus.Sprague-Dawley rats were ovariectomized at 21 days of age. Diabetes, defined as blood glucose levels greater than 300mg%, was induced in half of these animals by the injection of 85mg streptozotocin/kg body weight.The remaining animals were classified as normal. Animals from both groups were injected with either 0.9% saline or 4μg E2/100gm body weight. At 18, 24, 36 and 48 hours post-injection of E2, uterine segments were collected from 8-10 animals in each experimental group and processed for electron microscopic examination. Uterine segments from saline-injected animals served as controls. Data summarized describe observations made from thin-sections and have not been extrapolated for whole cells.

Morphologic Study of Carcinoid-Like Tumors and Their Relation to True Carcinoids, Using Tumors of the Breast as a Model

1983 ◽  
Vol 69 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Faripour A. Forouhar
Keyword(s):  
Ductal Carcinoma ◽  
Secretory Granules ◽  
Lobular Carcinoma ◽  
Prognostic Significance ◽  
Electron Microscopic Examination ◽  
Carcinoid Tumors ◽  
Electron Microscopic ◽  
Microscopic Evaluation ◽  
Microscopic Studies ◽  
Membrane Bound

Two cases of an unusual type of infiltrating ductal carcinoma of the breast are presented. Both cases demonstrated a carcinoid-like pattern and were indistinguishable from carcinoid tumors of the breast by light microscopy. However, Grimelius stains and electron microscopic evaluation showed no evidence of membrane bound secretory granules. In regard to the prognostic significance and proper classification of carcinoids of the breast, awareness of carcinoid-like morphologic variants of infiltrating ductal or lobular carcinoma is important. It is also apparent that there is a spectrum of tumors which demonstrate some properties of true carcinoids, however, only the true carcinoids show a better prognosis and it serves no useful purpose to separate the rest of these tumors. The diagnosis of carcinoid tumors requires demonstration of secretory granules on electron microscopic examination or in special stains; conventional light microscopic studies alone are insufficient for this diagnosis. All these principles may be applied to carcinoid like tumors of other sites.

Beaded nerve terminals in feline laryngeal mucosa

1998 ◽  
Vol 119 (1) ◽  
pp. 113-116 ◽  
Author(s):  
Kuniyoshi Tsuda ◽  
Tadatsugu Maeyama ◽  
Takemoto Shin
Keyword(s):  
Secretory Granules ◽  
Electron Microscopic Examination ◽  
Nerve Fibers ◽  
Nerve Terminals ◽  
Electron Microscopic ◽  
Epithelial Basement Membrane ◽  
Laryngeal Mucosa ◽  
Immunohistochemical Techniques ◽  
Airway Defense ◽  
Defense Reflex

To investigate the mechanism of airway defense reflex, beaded nerve terminals were studied by immunohistochemical techniques. In the supraglottic region the density of PGP 9.5–immunoreactive nerve fibers was the highest at the base of the glottic surface in the epiglottis, and in the glottic region it was the highest in the arytenoid region. In the subglottic region the number of positive nerve fibers was less than the number at the base of the glottic surface in the epiglottis, and when the laryngeal mucosa was processed with NaOH to dissolve the epithelium, it was possible to observe beaded nerve terminals more clearly. These beaded nerve terminals were found just beneath, in the epithelial basement membrane. Electron microscopic examination of beaded nerve terminals revealed a large quantity of secretory granules and mitochondria, suggesting that their structure is similar to that of nerve terminals. Thus these beaded nerve terminals may function as mechanoreceptors. (Otolaryngol Head Neck Surg 1998;119:113–6.)

THE ULTRASTRUCTURE OF THE ACCESSORY SEX ORGANS OF THE MALE RAT

1973 ◽  
Vol 74 (3) ◽  
pp. 605-614 ◽  
Author(s):  
Erik Dahl ◽  
Kjell J. Tveter ◽  
Åsmund Kjaerheim
Keyword(s):  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Secretory Granules ◽  
Electron Microscopic Examination ◽  
Cell Types ◽  
Male Rat ◽  
Electron Microscopic ◽  
Genital Complex ◽  
Accessory Sex Organs ◽  
Complete Regeneration

ABSTRACT The present paper describes the fine structure of the accessory sex organs of the male rat as seen after stimulation with testosterone administered to castrated adults as well as infantile rats. Electron microscopic examination of the testosterone-treated castrated animals revealed an almost complete regeneration of the epithelial cells in all the organs, with a well developed Golgi area, a well developed rough endoplasmic reticulum and an increased number of secretory granules. The results obtained are discussed in relation to the biochemical data, and it is concluded that the various cell types of the accessory genital complex react in a fundamentally similar manner to the administration of testosterone.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

Ultrastructural Aspects of Golgi Complex Function in Parathyroid Cells of Winter Frogs

1973 ◽  
Vol 31 ◽  
pp. 680-681
Author(s):  
William J. Dougherty
Keyword(s):  
Golgi Complex ◽  
Secretory Granules ◽  
Complex Function ◽  
Secretory Activity ◽  
Secretory Proteins ◽  
Perivascular Spaces ◽  
Pituitary Cells ◽  
Electron Microscopic ◽  
Ca Ions ◽  
Regulation Of Secretion

The regulation of secretion in exocrine and endocrine cells has long been of interest. Electron microscopic and other studies have demonstrated that secretory proteins synthesized on ribosomes are transported by the rough ER to the Golgi complex where they are concentrated into secretory granules. During active secretion, secretory granules fuse with the cell membrane, liberating and discharging their contents into the perivascular spaces. When secretory activity is suppressed in anterior pituitary cells, undischarged secretory granules may be degraded by lysosomes. In the parathyroid gland, evidence indicates that the level of blood Ca ions regulates both the production and release of parathormone. Thus, when serum Ca is low, synthesis and release of parathormone are both stimulated; when serum Ca is elevated, these processes are inhibited.

A Comparative Study of Fractographic Replicas

1974 ◽  
Vol 32 ◽  
pp. 566-567
Author(s):  
Loren Anderson ◽  
Pat Pizzo ◽  
Glen Haydon
Keyword(s):  
Electron Microscopy ◽  
Electron Microscopic Examination ◽  
Direct Examination ◽  
Electron Microscopic ◽  
Reliable Technique ◽  
Service Failures ◽  
Transmission Electron ◽  
Mode Of Failure ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Transmission electron microscopy of replicas has long been used to study the fracture surfaces of components which fail in service. Recently, the scanning electron microscope (SEM) has gained popularity because it allows direct examination of the fracture surface. However, the somewhat lower resolution of the SEM coupled with a restriction on the sample size has served to limit the use of this instrument in investigating in-service failures. It is the intent of this paper to show that scanning electron microscopic examination of conventional negative replicas can be a convenient and reliable technique for determining mode of failure.

Novel methods for demonstrating osseointegration of hydroxylapatite-coated titanium hip prostheses

1991 ◽  
Vol 49 ◽  
pp. 34-35 ◽  
Author(s):  
P. Frayssinet ◽  
J. Hanker ◽  
D. Hardy ◽  
B. Giammara
Keyword(s):  
Hip Prosthesis ◽  
Ethanol Concentration ◽  
Bone Matrix ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Hip Prostheses ◽  
Cellular Inclusions ◽  
Microscopic Studies ◽  
Diamond Saw ◽  
Plasma Sprayed

Prostheses implanted in hard tissues cannot be processed for electron microscopic examination or microanalysis in the same way as those in other tissues. For these reasons, we have developed methods allowing light and electron microscopic studies as well as microanalysis of the interface between bone and a metal biomaterial coated by plasma-sprayed hydroxylapatite(HA) ceramic.An HA-coated titanium hip prosthesis (Corail, Landos, France), which had been implanted for two years, was removed after death (unrelated to the orthopaedic problem). After fixation it was dehydrated in solutions of increasing ethanol concentration prior to embedment in polymethylmethacrylate(PMMA). Transverse femur sections were obtained with a diamond saw and the sections then carefully ground to a thickness of 200 microns. Plastic-embedded sections were stained for calcium with a silver methenamine modification of the von Kossa method for calcium staining and coated by carbon. They have been examined by back-scatter SEM on an ISI-SS60 operated at 25 KV. EDAX has been done on cellular inclusions and extracellular bone matrix.

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