scholarly journals Metabolic impact of pheochromocytoma/paraganglioma: targeted metabolomics in patients before and after tumor removal

2019 ◽  
Vol 181 (6) ◽  
pp. 647-657
Author(s):  
Zoran Erlic ◽  
Max Kurlbaum ◽  
Timo Deutschbein ◽  
Svenja Nölting ◽  
Aleksander Prejbisz ◽  
...  
Keyword(s):  
Acid Metabolism ◽  
Urinary Catecholamine ◽  
Targeted Metabolomics ◽  
Tumor Removal ◽  
Chromatography Tandem Mass Spectrometry ◽  
Catecholamine Excess ◽  
Before And After ◽  
Liquid Chromatography Tandem Mass ◽  
Tumor Phenotype ◽  
Total Urine

Objective Excess catecholamine release by pheochromocytomas and paragangliomas (PPGL) leads to characteristic clinical features and increased morbidity and mortality. The influence of PPGLs on metabolism is ill described but may impact diagnosis and management. The objective of this study was to systematically and quantitatively study PPGL-induced metabolic changes at a systems level. Design Targeted metabolomics by liquid chromatography-tandem mass spectrometry of plasma specimens in a clinically well-characterized prospective cohort study. Methods Analyses of metabolic profiles of plasma specimens from 56 prospectively enrolled and clinically well-characterized patients (23 males, 33 females) with catecholamine-producing PPGL before and after surgery, as well as measurement of 24-h urinary catecholamine using LC-MS/MS. Results From 127 analyzed metabolites, 15 were identified with significant changes before and after surgery: five amino acids/biogenic amines (creatinine, histidine, ornithine, sarcosine, tyrosine) and one glycerophospholipid (PCaeC34:2) with increased concentrations and six glycerophospholipids (PCaaC38:1, PCaaC42:0, PCaeC40:2, PCaeC42:5, PCaeC44:5, PCaeC44:6), two sphingomyelins (SMC24:1, SMC26:1) and hexose with decreased levels after surgery. Patients with a noradrenergic tumor phenotype had more pronounced alterations compared to those with an adrenergic tumor phenotype. Weak, but significant correlations for 8 of these 15 metabolites with total urine catecholamine levels were identified. Conclusions This first large prospective metabolomics analysis of PPGL patients demonstrates broad metabolic consequences of catecholamine excess. Robust impact on lipid and amino acid metabolism may contribute to increased morbidity of PPGL patients.

scholarly journals Application of a Liquid Chromatography Tandem Mass Spectrometry Method for the Simultaneous Detection of Seven Allergenic Foods in Flour and Bread and Comparison of the Method with Commercially Available ELISA Test Kits

2011 ◽  
Vol 94 (4) ◽  
pp. 1060-1068 ◽  
Author(s):  
Julia Heick ◽  
Markus Fischer ◽  
Sandra Kerbach ◽  
Ulrike Tamm ◽  
Bert Popping
Keyword(s):  
Simultaneous Detection ◽  
Elisa Test ◽  
Spectrometry Method ◽  
Chromatography Tandem Mass Spectrometry ◽  
Model Matrix ◽  
Before And After ◽  
Liquid Chromatography Tandem Mass ◽  
Novel Method ◽  
Detection Capabilities ◽  
Baked Products

Abstract To protect the allergic consumer, analytical methods need to be capable of detecting allergens in fnished products that typically contain multiple allergens. An LC/MS/MS method for simultaneous detection of seven allergens was developed and compared with commercially available ELISA kits. The detection capabilities of this novel method were demonstrated by analyzing incurred material containing milk, egg, soy, peanut, hazelnut, walnut, and almond. Bread was chosen as a model matrix. To assess the influence of baking on the method’s performance, analysis was done before and after baking. The same samples were analyzed with ELISA test kits from ELISA Systems, Morinaga, Neogen, and r-Biopharm. Peanut, hazelnut, walnut, and almond could be detected with both ELISA and LC/MS/MS regardless of whether the product was baked or not. LC/MS/MS clearly showed superior detection of milk in processed matrixes compared to ELISA, which exhibited signifcantly lower sensitivities when analyzing the baked products. Similar results were obtained when analyzing egg; however, one kit was capable of detecting egg in the processed samples as well.

Circulating free nitrotyrosine in obstructive sleep apnea

2004 ◽  
Vol 287 (2) ◽  
pp. R284-R287 ◽  
Author(s):  
Anna Svatikova ◽  
Robert Wolk ◽  
Hui H. Wang ◽  
Maria E. Otto ◽  
Kevin A. Bybee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Nitrosative Stress ◽  
Control Group ◽  
Chromatography Tandem Mass Spectrometry ◽  
Obstructive Sleep ◽  
Before And After ◽  
Liquid Chromatography Tandem Mass ◽  
And Oxidative Stress

Obstructive sleep apnea (OSA) has been increasingly linked to cardiovascular disease, endothelial dysfunction, and oxidative stress, generated by repetitive nocturnal hypoxemia and reperfusion. Circulating free nitrotyrosine has been reported as a novel biomarker of nitric oxide (NO)-induced oxidative/nitrosative stress. Nitrosative stress has been implicated as a possible mechanism for development of cardiovascular diseases. We tested the hypothesis that repetitive severe hypoxemia resulting from OSA would increase NO-mediated oxidative stress. We studied 10 men with newly diagnosed moderate to severe OSA who were free of other diseases, had never been treated for OSA, and were taking no medications. Nitrotyrosine measurements, performed by liquid chromatography-tandem mass spectrometry, were made before and after untreated apneic sleep. We compared free nitrotyrosine levels in these patients with those obtained at similar times in 10 healthy male control subjects without OSA, with similar age and body mass index. Evening baseline nitrotyrosine levels were similar before sleep in the control and OSA groups [0.16 ± 0.01 and 0.15 ± 0.01 ng/ml, respectively, P = not significant (NS)]. Neither normal nor disturbed apneic sleep led to significant changes of plasma nitrotyrosine (morning levels: control group 0.14 ± 0.01 ng/ml; OSA group 0.15 ± 0.01 ng/ml, P = NS). OSA was not accompanied by increased circulating free nitrotyrosine either at baseline or after sleep. This observation suggests that repetitive hypoxemia during OSA does not result in increased NO-mediated oxidative/nitrosative stress in otherwise healthy subjects with OSA.

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