scholarly journals Concentrations of steroid hormones, and of prolactin, in washings of the human uterus during the menstrual cycle

Reproduction ◽  
1986 ◽  
Vol 78 (1) ◽  
pp. 21-25 ◽  
Author(s):  
B. A. Stone ◽  
O. M. Petrucco ◽  
R. F. Seamark ◽  
B. M. Godfrey
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
Human Uterus

CHANGES IN THE CONCENTRATION OF PITUITARY AND STEROID HORMONES IN THE FOLLICULAR FLUID OF HUMAN GRAAFIAN FOLLICLES THROUGHOUT THE MENSTRUAL CYCLE

1975 ◽  
Vol 64 (3) ◽  
pp. 555-571 ◽  
Author(s):  
K. P. McNATTY ◽  
W. M. HUNTER ◽  
A. S. McNEILLY ◽  
R. S. SAWERS
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
Follicular Fluid ◽  
Follicle Stimulating Hormone ◽  
Secretory Activity ◽  
Follicular Phase ◽  
Hormonal Changes ◽  
Peripheral Plasma ◽  
Before And After ◽  
Late Follicular Phase

SUMMARY The concentrations of FSH, LH, prolactin, oestradiol and progesterone were measured in peripheral plasma and follicular fluid of women throughout the menstrual cycle. With the exception of prolactin, concentrations of pituitary and steroid hormones in follicular fluid correlated with those in peripheral plasma. Follicle-stimulating hormone was present in a greater number of small follicles ( < 8 mm) during or just after the peaks of FSH in peripheral plasma. During the mid-follicular phase the concentration of both FSH and oestradiol in fluid from large follicles ( ≥ 8 mm) was high. During the late follicular phase the large follicles ( ≥ 8 mm) contained high amounts of progesterone in addition to oestradiol, low physiological levels of prolactin, and concentrations of LH and FSH about 30 and 60% respectively of those found in plasma. By contrast no large 'active' follicles ( ≥ 8 mm) were found during the luteal phase although many contained both LH and FSH. Luteinizing hormone was present in a proportion of small follicles ( < 8 mm) during the late follicular and early luteal but not at other stages of the menstrual cycle. It is suggested that a precise sequence of hormonal changes occur within the microenvironment of the developing Graafian follicle; the order in which they occur may be of considerable importance for the growth of that follicle and secretory activity of the granulosa cells both before and after ovulation.

191 TRANSIENT RECEPTOR POTENTIAL SUPERFAMILY OF ION CHANNELS, TRPV6, IS CONSTITUTIVELY EXPRESSED AND REGULATED BY ESTROGEN IN THE HUMAN UTERUS DURING THE MENSTRUAL CYCLE

2011 ◽  
Vol 23 (1) ◽  
pp. 196
Author(s):  
Y.-K. Kim ◽  
H. Yang ◽  
E.-B. Jeung
Keyword(s):  
Ion Channels ◽  
Menstrual Cycle ◽  
Brush Border Membrane ◽  
Transient Receptor Potential ◽  
Prostate Gland ◽  
Receptor Potential ◽  
Human Uterus ◽  
Proximal Small Intestine ◽  
Transient Receptor ◽  
Human Menstrual Cycle

Two highly selective calcium channels at the apical sides of cells, members of the transient receptor potential (TRP) superfamily of ion channels (TRPV6 and TRPV5), are the main calcium ion entry channels. Previously, the location of TRPV6 has been described in the intestine in several species, including humans. It is located in the apical brush-border membrane of the intestinal enterocyte, where it regulates calcium entry into the cell. It is most abundant in the proximal small intestine (duodenum and jejunum), where calbindin and the calcium-pumping ATPase are also found. The TRPV6 calcium transporter is also found in the human placenta, pancreas, and prostate gland in some species. However, TRPV6 expression and its potential roles remain to be clarified in the endometrium of humans during the menstrual cycle. In this study, we used a human endometrial model to examine the expression of TRPV6 and its potential roles in the human menstrual cycle. A significant increase (1.5-fold) in the TRPV6 transcript and protein was observed in the human uterus at the proliferation phase compared with other phases. In addition, the spatial localization of TRPV6 in the human uterus was determined by immunohistochemistry. Uterine TRPV6 was abundantly localised in the cytoplasm of the endometrial and glandular epithelial cells in the menstrual phases. Overall, these results demonstrate that TRPV6 is abundantly expressed in human uterine tissue, suggesting that this protein may be involved in reproductive functions during the menstrual cycle in humans.

Platelet 3H-imipramine binding and steroid hormones serum concentrations during the menstrual cycle

1986 ◽  
Vol 88 (1) ◽  
pp. 86-89 ◽  
Author(s):  
Marie-France Poirier ◽  
Chawki Benkelfat ◽  
Anne-Marie Galzin ◽  
Salomon Z. Langer
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
Serum Concentrations ◽  
Imipramine Binding

scholarly journals Steroid Diagnostics of 21st Century in the Light of Their New Roles and Analytical Tools

2020 ◽  
pp. S193-S203 ◽  
Author(s):  
M. DUŠKOVÁ ◽  
L. KOLÁTOROVÁ ◽  
M. ŠIMKOVÁ ◽  
M. ŠRÁMKOVÁ ◽  
M. MALÍKOVÁ ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
21St Century ◽  
Reference Values ◽  
Current Technology ◽  
Amount Of Information ◽  
Analytical Phase ◽  
Analytical Tools ◽  
Fertile Women

The determination of steroid hormones and subsequent interpretation of results is accompanied by a range of difficulties. The amount of information that current technology can provide on the circulating concentrations of more than a hundred various steroid compounds can lead to problems with interpretation. The aim of this study is to help provide orientation in this maze of data on steroid hormones. First we focus on specific aspects arising from the pre-analytical phase of steroid determination that need to be considered when planning sampling, whether for diagnostics or research. Then, we provide a brief summary of the characteristics and diagnostic relevance of several steroid hormones and/or their metabolites: pregnenolone, 17α-hydroxy-pregnenolone, dehydroepiandrosterone, hydroxyderivatives of dehydroepiandrosterone, androstenedione, testosterone, estrone, estradiol, estriol, cortisol, cortisone, which in our institute are determined with validated LC-MS/MS methods. For these steroids, we also provide newly calculated reference values in fertile women according to the phase of their menstrual cycle.

232 DIFFERENTIAL EXPRESSION OF UTERINE CALCIUM-RELATED PROTEINS (NCKX3, NCX1, PMCA1, TRPV6, AND CABINDIN-D9k AND -D28k) DURING HUMAN MENSTRUAL CYCLE

2010 ◽  
Vol 22 (1) ◽  
pp. 274
Author(s):  
K. C. Choi ◽  
E. B. Jeung
Keyword(s):  
Menstrual Cycle ◽  
Medical Center ◽  
Expression Patterns ◽  
Embryo Implantation ◽  
Calcium Balance ◽  
Human Uterus ◽  
Secretory Phase ◽  
Total N ◽  
Proliferative Phase ◽  
Related Proteins

The endometrium is hostile to embryo implantation except during the window of receptivity. A change in endometrial gene expression is required for the development of receptivity. The uterine calcium balance is crucial for physiological functioning, including smooth muscle contraction and embryo implantation. The location of cytoplasmic calcium-related proteins (CRP) include the calcium transporters 1 (CaT1), calbindin-D9k/-D28k (CaBP- 9k/28k), plasma membrane Ca2+-ATPase 1b (PMCA1b), sodium/calcium exchangers (NCX1), and potassium-dependent Na+/Ca2+ exchanger (NCKX3). The expressions of these CRP and their potential roles in the uterus of human during the menstrual cycle remain to be clarified. Thus, in this current study, the expression patterns of CRP were examined for their roles in the human uterus during the menstrual cycle. Human endometrial tissues were collected by curettage from women undergoing hysteroscopy for investigation of tubal patency or tubal ligation. Approval was given by the Human Ethics Committee at SCH Medical Center, Bucheon, and signed consent was obtained in every case. Human uterus (total n = 51) were divided into 3 groups: menstrual, proliferative, and secretory phase. Reverse-transcription PCR and Western blot analysis were applied to measure the level of CRP mRNA and protein, respectively. During the menstrual cycle of human, the expression levels of CaT1 mRNA and protein were increased 5-fold at proliferative phase (Days 6 to 13) compared with secretory phase in the endometrium of uterus. The expression of CaBP-28k mRNA and protein was less 2-fold during the proliferative phase (Days 6 to 13) than during the secretory phase (Days 16 to 28). However, the expressions of NCX1, NCKX3, and PMCA1b mRNA and protein were not altered during cycle, whereas the expression of CaBP-9k was not observed in the uterus of human. In addition, spatial expression of CRP was detected by immunohistochemistry Uterine CRP was abundantly localized in the cytoplasm of the luminal and glandular epithelial cells during menstrual cycle. Taken together, these results indicate that uterine CRP is abundantly expressed in the uterus, suggesting that uterine expression of CRP might be involved in reproductive function during the menstrual cycle in human.

Fundamental Frequency Variations Across the Menstrual Cycle and the Use of an Oral Contraceptive Pill

2020 ◽  
Vol 63 (4) ◽  
pp. 1033-1043
Author(s):  
Filipa M. B. Lã ◽  
Nuria Polo
Keyword(s):  
Oral Contraceptive ◽  
Standard Deviation ◽  
Menstrual Cycle ◽  
Steroid Hormones ◽  
Fundamental Frequency ◽  
Oral Contraceptive Pill ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Contraceptive Pill ◽  
The Voice

Purpose Concentrations of sex steroid hormones—estrogens, progesterone, and testosterone—have been associated with premenstrual and menstrual vocal symptoms. However, the extent to which these symptoms may be reflected on acoustical features of the voice is still debated. This study investigates variations in fundamental frequency ( f o ) and related parameters in connected speech across phases of the menstrual cycle and during the use of a combined oral contraceptive pill (OCP). Method Electrolaryngographic recordings were made, and blood samples were collected at three different phases of the menstrual cycle—menstrual, follicular, and luteal—for placebo and OCP use. These two conditions were blindly and randomly allocated in the study. Speaking f o (SFF), SFF standard deviation, SFF rate of change, SFF slope, maximum and minimum f o , and f o range were extracted for nine healthy females while reading a phrase from the Rainbow Passage. Concentrations of sex hormones were analyzed in serum. Nonparametric statistical tests were carried out to assess differences between phases and conditions. Results SFF, its standard deviation, and maximum f o were significantly different between phases of the menstrual cycle for placebo use only. Menstrual phase showed the lowest values. Maximum and minimum f o were significantly different between placebo and OCP use for menstrual and follicular phases, respectively. Conclusions Fluctuations in sex steroid hormones across the menstrual cycle alter f o in speech more than a particular hormonal concentration. OCP use seems to have a stabilizing effect on the voice relative to f o and related parameters in speech.

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