scholarly journals Effects of erythropoietin on ischemia, follicular survival, and ovarian function in ovarian grafts

Reproduction ◽  
2014 ◽  
Vol 147 (5) ◽  
pp. 733-741 ◽  
Author(s):  
Monireh Mahmoodi ◽  
Malek Soleimani Mehranjani ◽  
Seyed Mohammad Ali Shariatzadeh ◽  
Hussein Eimani ◽  
Abdulhussein Shahverdi
Keyword(s):  
Ovarian Function ◽  
Ischemia Reperfusion ◽  
Ovarian Tissue ◽  
Treated Group ◽  
Saline Group ◽  
Ovarian Tissue Transplantation ◽  
The Mean ◽  
And Function ◽  
One Way Anova ◽  
Plasma Malondialdehyde

Ovarian tissue transplantation is performed to preserve fertility in patients undergoing chemotherapy and radiotherapy. However, ischemia/reperfusion (IR) injury and free radical production occurring during the revascularization of the transplanted tissue are the major limitations of this procedure. The aim of this study was to investigate the effect of erythropoietin (EPO) as an antioxidant on oxidative stress and ovary survival following transplantation. The Naval Medical Research Institute (NMRI) mice (4–5 weeks old) were divided into three groups (six mice per group): control; autograft+saline, and autograft+EPO (500 IU/kg i.p.). After 28 days, ovary compartments were estimated stereologically. DNA fragmentation and plasma malondialdehyde (MDA), progesterone, and estradiol (E2) concentrations were also evaluated. The results were analyzed using one-way ANOVA and Tukey's test, and the means were significantly different at P<0.05. The mean total volume of ovary, cortex, and medulla and the number of follicles increased significantly in the autograft+EPO group (P<0.01). Apoptosis rate in the autograft+EPO group was lower than that in the autograft+saline group. The concentration of MDA decreased significantly in the autografted EPO-treated group than in the autografted saline-treated group (P<0.01). The concentration of E2 increased significantly in the autograft+EPO group than in the autograft+saline group (P<0.01). EPO reduced IR injury, increasing follicle survival and function in grafted ovaries.Free Persian abstractA Persian (Farsi) translation of the abstract is freely available online at http://www.reproduction-online.org/content/147/5/733/suppl/DC1

scholarly journals Effects of L-Carnitine on follicular reserve and Caspase-3 in transplanted mouse ovarian tissue

2019 ◽  
Author(s):  
Fatemeh Shahi Sadrabadi ◽  
Kazem Parivar ◽  
Hussein Imani ◽  
Abdolhussein Shahverdi
Keyword(s):  
Caspase 3 ◽  
Sham Group ◽  
Ischemia Reperfusion ◽  
Ovarian Tissue ◽  
Control Group ◽  
Ovarian Tissue Transplantation ◽  
Study Results ◽  
Significant Difference ◽  
Follicular Reserve ◽  
One Way Anova

Introdution: One of the major challengs in ovarian tissue transplantation is overcomeing ischemia/ reperfusion injuries. During ischemia–reperfusion processes, oxygen free radicals constitute the most important component that induces damage of the grafted tissues. The aim of this study was to investigate the effect of L-Carnitine (LC) as an antioxidant on heterotopic transplantation of mouse ovarian tissue. Methods: In this laboratory experimental study, 5- week old female NMRI mice were divided into four groups: control, transplanted without administration (autograft), sham group (autograft+ saline) and LC group (autograft+ L- carnitine). Left ovarian tissues were transplanted into the Gluteal muscle for 3 weeks. After this time, ovarian tissues from all groups were removed and fixed in formalin for histological studies. Furthermore, rate of Caspase- 3 was assessed by immunohistochemistry test. Lipid peroxidation was assessed by measuring  malondialdehyde (MDA). One-way ANOVA and Tudey test was used to analyze the data using the spss 16 software. Significance was defined as P≤0.0. Results: The study results indicated that total follicular count in transplantedwithout administration and sham groups was significantly lower than the control group (p<0.05), but there was no significant difference between the control and LC groups. In addition, the rate of caspase-3 was decreased in the LC group, but no significant difference existed between all groups (p<0.05). A significant reduction in the concentration of MDA was observed in the LC group than that in the other transplanted groups (p<0.05). Conclusion: In this study, LC could improve the ovarian reserve to some extent, but its effect was not significant.

scholarly journals Effects of prophylactic ketamine and pethidine to control postanesthetic shivering: A comparative study

2018 ◽  
Vol 5 (12) ◽  
pp. 2898-2903 ◽  
Author(s):  
Masoum Khoshfetrat ◽  
Ali Rosom Jalali ◽  
Gholamreza Komeili ◽  
Aliakbar Keykha
Keyword(s):  
Normal Saline ◽  
Pearson Correlation ◽  
Saline Group ◽  
Normal Saline Group ◽  
Chi Square ◽  
Double Blind ◽  
Postanesthetic Shivering ◽  
Significant Difference ◽  
The Mean ◽  
One Way Anova

Background: Shivering is an undesirable complication following general anesthesia and spinal anesthesia, whose early control can reduce postoperative metabolic and respiratory complications. Therefore, this study aims to compare the effects of prophylactic injection of ketamine and pethidine on postoperative shivering. Methods: This double-blind clinical trial was performed on 105 patients with short-term orthopedic and ENT surgery. The patients were randomly divided into three groups; 20 minutes before the end of the surgery, 0.4 mg/kg of pethidine was injected to the first group, 0.5 mg/kg of ketamine was injected to the second group, and normal saline was injected to the third group. After the surgery, the tympanic membrane temperature was measured at 0, 10, 20, and 30 minutes. The shivering was also measured by a four-point grading from zero (no shivering) to four (severe shivering). Data were analyzed by one-way ANOVA, Kruskal Wallis, Chi-square and Pearson correlation. Results: The mean age of patients was 35.8+/-11.45 years in the ketamine group, 34.8+/-11.64 years in the normal saline group, and 33.11+/-10.5 years in the pethidine group. The one-way ANOVA showed no significant difference in the mean age between the three groups (P=0.645). The incidence and intensity of shivering were significantly higher in the normal saline group than in the ketamine and pethidine groups (p=0.001). However, there was no significant difference in the incidence and the intensity of shivering between the ketamine and the pethidine groups (p=0.936). Conclusion: The results showed that the 0.5 mg/kg of ketamine could control the post-anesthetic shivering.  

scholarly journals FERTILITY PRESERVATION: Follicle reserve loss in ovarian tissue transplantation

Reproduction ◽  
2019 ◽  
Vol 158 (5) ◽  
pp. F35-F44 ◽  
Author(s):  
Hadassa Roness ◽  
Dror Meirow
Keyword(s):  
Ovarian Function ◽  
Ovarian Tissue ◽  
Young Patients ◽  
Ovarian Tissue Cryopreservation ◽  
Ischemic Damage ◽  
Pharmacological Agents ◽  
Ovarian Tissue Transplantation ◽  
After Cancer ◽  
Tissue Cryopreservation ◽  
Follicle Activation

Ovarian tissue cryopreservation and transplantation (OTCP-TP) has progressed over the past decade from a revolutionary experimental procedure to a well-accepted treatment in many centers for young patients with a high risk of ovarian failure after cancer treatment. The procedure is remarkably successful, with studies reporting return of ovarian function in up to 95% of graft recipients and pregnancy rates of between 30 and 50%. The most significant limitation of OTCP-TP is the massive loss of follicles that occurs following transplantation, which is primarily attributed to ischemic damage and follicle activation. We review the current approaches to reducing follicle loss and maximizing graft lifespan via pharmacological agents which reduce ischemic damage and follicle activation. We further discuss the value and disadvantage of inducing follicle activation in the graft as a means of generating mature follicles in the immediate short term.

scholarly journals Effect of Resveratrol on Mouse Ovarian Cryopreservation and Transplantation

2021 ◽  
Author(s):  
Dalin Wang ◽  
Menghui Geng ◽  
Dongying Gan ◽  
Gege Han ◽  
Gao Gao ◽  
...  
Keyword(s):  
Free Radicals ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Ovarian Tissue ◽  
Ovarian Follicle ◽  
Saline Group ◽  
Endocrine Function ◽  
Qrt Pcr ◽  
Anti Inflammatory

Abstract BackgroundAfter ovarian tissue transplantation, ischemia-reperfusion injury and free radicals cause follicle depletion and apoptosis. Therefore, the use of antioxidants to reduce the production of free radicals is an important method to address the consequences of ischemia-reperfusion injury. Resveratrol is a natural active polyphenol compound with anti-inflammatory, antitumor, strong antioxidant and anti-free radical properties. The aim of this study was to investigate whether resveratrol could improve the effect of autologous ovarian transplantation after cryopreserve-thawn mouse ovarian tissue.MethodsWhole-ovary vitrification and autotransplantation models were used to investigate the effects of resveratrol. Six-week-old female mice from the Institute of Cancer Research (ICR) were subjected to vitrification. All ovaries were preserved in liquid nitrogen for 1 week before being thawed. After thawing, ovarian tissues were autotransplanted in the bilateral kidney capsules. Mice (n=72) were randomly divided into four groups to determine the optimal concentration of resveratrol (experiment I). Treatments were given as follows: saline, 5 mg/kg resveratrol, 15 mg/kg resveratrol and 45 mg/kg resveratrol, which were administered orally for one week. Grafted ovaries were collected for analysis on days 3, 7, and 21 after transplantation. Ovarian follicle morphology was assessed by hematoxylin and eosin staining. Serum FSH and E2 levels were measured to estimate the transplanted ovarian reserve and endocrine function. Other mice were randomly divided into two groups—saline and 45 mg/kg resveratrol to further evaluate the effect of resveratrol and explore the mechanisms underlying this effect (experiment II). Ovarian follicle apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays. Immunohistochemistry, qRT-PCR and western blotting (MDA, SOD, NF-κB, IL-6 and SIRT1) were used to explore the mechanisms of resveratrol. Moreover, oocytes derived from autotransplanted ovaries at 21 days were cultured and fertilized in vitro. ResultsThe proportions of morphologically normal (G1) follicles at 3, 7 and 21 days were significantly higher in the 45 mg/kg resveratrol group than in the saline group. The TUNEL-stained follicles (%) at 7 days were significantly decreased in the 45 mg/kg resveratrol group compared with the saline group. Western blot analysis revealed that SOD2 and SIRT1 levels were significantly higher in the 45mg/kg resveratrol group than in the saline group at day 7 and that MDA and NF-κB levels were lower in the saline group on day 3. Likewise, IL-6 was lower in the saline group on day 7. These results are basically consistent with the qRT-PCR results. In addition, the mean number of retrieved oocytes and fertilization and cleavage were significantly increased in the 45 mg/kg resveratrol group compared with the saline group. ConclusionsAdministration of resveratrol could improve the quality of cryopreserved mouse ovarian tissue after transplantation and the embryo outcome, through anti-inflammatory and antioxidative mechanisms.

Ovarian Tissue Vitrification as a Method for Ovarian Preservation in Women with Cancer: an Analysis of Granulose Cell Apoptosis

KnE Medicine ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Budi Wiweko
Keyword(s):  
Experimental Study ◽  
Cell Apoptosis ◽  
Ovarian Function ◽  
Ovarian Tissue ◽  
Obstetrics And Gynecology ◽  
Human Ovary ◽  
Primordial Follicles ◽  
Quasi Experimental ◽  
Function Preservation ◽  
The Mean

<p class="ParaAttribute48">The aim of this study was to obtain the effective method of ovarian function preservation with granulose cell apoptosis assessment. Ovarian tissue vitrification became a method for ovarian function preservation in women with cancer. This technique can be done anytime without delay on cancer therapy, in prepubertal and unmarried patient. It also can store many primordial follicles. Ovarian tissue vitrification study is still limited to animal test and there was no data about apoptosis assessment after ovarian vitrification in human ovary. This is a quasi experimental study which was held in Department of Obstetrics and Gynecology Faculty of Medicine Universitas Indonesia - Dr. Cipto Mangunkusumo General Hospital and Fatmawati Hospital Jakarta from March 2012 to May 2015. Ovaries from thirteen women between 31-37 years of age who underwent oophorectomy with gynecological indication were examined. There were no difference morphologically between follicles from fresh and warmed-vitrified ovaries. The mean protein Bax expression on the fresh ovaries which assessed in the form of H-score was 1,66 ± 0,14 compared to 1,68 ± 0,13 on the warmed-vitrified grup (p = 0,165). The mean protein Bcl-2 expression on the fresh ovaries which assessed in the form of H-score was 1,73 ± 0,10 compared to 1,71 ± 0,10 on the warmed-vitrified grup (p = 0,068). As a conclusion, it was shown that vitrification did not affect Bax and Bcl-2 expression on human ovary.</p>

scholarly journals Ovarian function and reproductive outcome after ovarian tissue transplantation: a systematic review

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Sepideh Sheshpari ◽  
Mahnaz Shahnazi ◽  
Halimeh Mobarak ◽  
Shahin Ahmadian ◽  
Alberto Miranda Bedate ◽  
...  
Keyword(s):  
Systematic Review ◽  
Ovarian Function ◽  
Current Knowledge ◽  
Ovarian Tissue ◽  
Tissue Transplantation ◽  
Endocrine Function ◽  
Cochrane Library ◽  
Reproductive Outcome ◽  
Follicular Growth ◽  
Ovarian Tissue Transplantation

AbstractThe aim of this systematic review study is to summarize the current knowledge of ovarian tissue transplantation and provide insight on ovarian function, fertility and reproductive outcome following ovarian tissue transplantation. Relevant studies were identified by searching through PubMed, Cochrane Library, Embase, ProQuest, and Scopus databases until August 2018. Ovarian function by examination of the hormonal level was evaluated, together with follicular growth, the return of menstrual cycle and assessment of reproductive consequences: pregnancy, miscarriage rates and live birth after transplantation. Studies including female patients aged between 22 and 49 years that were subjected to ovarian tissue transplantation were considered. A total of 1185 studies were identified in the primary search. Titles and abstracts were screened for assessment of the inclusion criteria. Finally, twenty-five articles met the criteria and were included in this study. In general, 70% of patients that underwent ovarian tissue transplantation had ovarian and endocrine function restoration as well as follicular growth. Pregnancy was reported with 52% of the patients. The available evidence suggests that ovarian tissue transplantation is a useful and an applied approach to restore hormonal function, endocrine balance and eventually fertility outcomes in patients that are predisposed to lose their fertility, diagnosed with premature ovarian failure (POF), as well as women undergoing cancer treatments. Identification of the techniques with the lowest invasions for follicular and oocyte development after ovarian tissue transplantation aiming to reduce probable adverse effects after treatment is indispensable.

scholarly journals Expectations and limitations of ovarian tissue transplantation

Zygote ◽  
2017 ◽  
Vol 25 (4) ◽  
pp. 391-403 ◽  
Author(s):  
N.J. Donfack ◽  
K.A. Alves ◽  
V.R. Araújo ◽  
A. Cordova ◽  
J.R. Figueiredo ◽  
...  
Keyword(s):  
Premature Ovarian Failure ◽  
Ovarian Function ◽  
Ex Vivo ◽  
Ovarian Tissue ◽  
Ovarian Failure ◽  
Tissue Transplantation ◽  
Endocrine Function ◽  
Embryo Cryopreservation ◽  
Pelvic Cavity ◽  
Ovarian Tissue Transplantation

SummaryConstant progress in the diagnosis and treatment of cancer disease has increased the number and prognosis of cancer survivors. However, the toxic effects of chemotherapy and radiotherapy on ovarian function have resulted in premature ovarian failure. Patients are, therefore, still expecting methods to be developed to preserve their fertility successfully. Several potential options are available to preserve fertility in patients who face premature ovarian failure, including immature or mature oocyte and embryo cryopreservation. However, for children or prepubertal women needing immediate chemotherapy, cryopreservation of ovarian tissue is the only alternative. The ultimate aim of this strategy is to implant ovarian tissue into the pelvic cavity (orthotopic site) or in a heterotopic site once oncological treatment is completed and the patient is disease free. Transplantation of ovarian tissue with sufficiently large numbers of follicles could potentially restore endocrine function and allow multiple cycles for conception. However, the success of ovarian tissue transplantation still has multiple challenges, such as the low number of follicles in the graft that may affect their longevity as well as the survival of the tissue during ex vivo processing and subsequent transplantation. Therefore, this review aims to summarize the achievements of ovary grafting and the potential techniques that have been developed to improve ovarian graft survival.

scholarly journals Hormonal Characteristics of Women Receiving Ovarian Tissue Transplantation with or without Endogenous Ovarian Activity

2021 ◽  
Vol 10 (22) ◽  
pp. 5217
Author(s):  
Vinnie Hornshøj Greve ◽  
Margit Dueholm ◽  
Linn Salto Mamsen ◽  
Stine Gry Kristensen ◽  
Erik Ernst ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fertility Preservation ◽  
Ovarian Reserve ◽  
Ovarian Function ◽  
Ovarian Tissue ◽  
Ovarian Tissue Cryopreservation ◽  
Ovarian Activity ◽  
Concomitant Increase ◽  
Ovarian Tissue Transplantation ◽  
Tissue Cryopreservation

Ovarian tissue cryopreservation (OTC) and transplantation of frozen/thawed ovarian tissue (OTT) are used for fertility preservation in girls and women. Here, we evaluated the hormonal characteristics of women with or without postmenopausal levels of FSH at the time of OTT to study differences and conditions that best support the initiation of ovarian function. A total of 74 women undergoing OTT (n = 51 with menopausal levels of FSH; n = 23 with premenopausal levels) were followed by measurements of FSH, LH, AMH, and oestradiol. Concentrations of FSH and LH returned to premenopausal levels after 20 weeks on average, with a concomitant increase in oestradiol. Despite resumption of ovarian activity, AMH concentrations were in most instances below the detection limit in the menopausal group, suggesting a low ovarian reserve. Despite a higher age in the premenopausal group, they more often experienced an AMH increase than the menopausal group, suggesting that conditions in the premenopausal ovary better sustain follicle survival, perhaps due to the higher concentrations of oestradiol. Collectively, this study highlights the need for improving follicle survival after OTT. Age and the amount of tissue transplanted are important factors that influence the ability to regain ovarian activity and levels of FSH may need to be downregulated and oestradiol increased prior to OTT.

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