Ultrastructural evidence of transplacental transport of immunoglobulin G in bitches

In dogs, passive immunity is conferred to fetuses and neonates by the transfer of maternal immunoglobulin G through the placenta during the last trimester of pregnancy and via the mammary gland after parturition, respectively. However, morphological evidence of transplacental transport is still lacking. The aim of the present study was to localize maternal immunoglobulin G in the labyrinthine zone and in the haemophagous zone of the canine placenta by means of immunohistochemistry and immunocytochemistry. In the labyrinthine zone, immunoglobulin G was detected in all the layers of the materno-fetal barrier including the fetal capillaries. Immunoreactivity was particularly prominent in maternal basement membrane material as well as in the syncytiotrophoblast. However, this evidence of transplacental transport of immunoglobulin G originated from a limited number of unevenly distributed maternal vessels only. In the cytotrophoblast of the haemophagous zone, immunoglobulin G was localized to phagolysosomes at various stages but was never detected within fetal vessels. The results indicate that maternal immunoglobulin G is degraded in cytotrophoblast cells of the hemophagous zone and, therefore, that transplacental transport is restricted to a subpopulation of maternal vessels in the labyrinthine zone.

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Targeted expression of stromelysin-1 in mammary gland provides evidence for a role of proteinases in branching morphogenesis and the requirement for an intact basement membrane for tissue-specific gene expression [published erratum appears in J Cell Biol 1996 Feb;132(4):following 752]

The Journal of Cell Biology ◽

10.1083/jcb.125.3.681 ◽

1994 ◽

Vol 125 (3) ◽

pp. 681-693 ◽

Cited By ~ 264

Author(s):

C. J. Sympson

Keyword(s):

Gene Expression ◽

Mammary Gland ◽

Basement Membrane ◽

Branching Morphogenesis ◽

Specific Gene ◽

Tissue Specific ◽

Tissue Specific Gene ◽

Specific Gene Expression ◽

Targeted Expression

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Massive myoepithelial proliferation (myoepitheliosis) with lumpy deposits of basement membrane material closely associated with apocrine adenosis and ductal carcinomain situof the breast

Pathology International ◽

10.1111/j.1440-1827.2011.02712.x ◽

2011 ◽

Vol 61 (10) ◽

pp. 615-617

Author(s):

Tomonori Kawasaki ◽

Toshio Oyama ◽

Hiroshi Nakagomi ◽

Kazushige Furuya ◽

Tetsuo Kondo ◽

...

Keyword(s):

Basement Membrane ◽

Membrane Material

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Spherulosis of the Breast

Archives of Pathology & Laboratory Medicine ◽

10.5858/1999-123-0626-sotb ◽

1999 ◽

Vol 123 (7) ◽

pp. 626-630 ◽

Cited By ~ 1

Author(s):

Eoghan E. Mooney ◽

Naila Kayani ◽

Fattaneh A. Tavassoli

Keyword(s):

Basement Membrane ◽

Invasive Carcinoma ◽

Armed Forces ◽

Membrane Material ◽

Material Deposition ◽

Immunohistochemical Profile

Abstract Objective.—Collagenous spherulosis of the breast is an uncommon localized pattern of basement membrane material deposition that may be mistaken for atypical proliferations or carcinoma. This report describes 9 cases in which the predominant or exclusive appearance of the spherules was basophilic instead of eosinophilic. Design.—The files of all cases of collagenous spherulosis diagnosed at the Armed Forces Institute of Pathology were reviewed to ascertain the frequency of diagnosis. Results.—Spherulosis with a predominantly basophilic pattern had a histochemical and immunohistochemical profile similar to collagenous spherulosis and was associated with more collagenous-appearing forms in 7 of 9 cases. Review of 81 cases showed that collagenous spherulosis was correctly diagnosed in 15% of referrals and was mistaken for intraductal or invasive carcinoma in 11% of cases. Conclusions.—Mucinous and collagenous patterns appear to be related forms of spherulosis. They are underrecognized by pathologists and maybe mistaken for atypia or malignancy.

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Enhanced synthesis of gelatinase and stromelysin by myoepithelial cells during involution of the rat mammary gland.

Journal of Histochemistry & Cytochemistry ◽

10.1177/40.5.1315355 ◽

1992 ◽

Vol 40 (5) ◽

pp. 697-703 ◽

Cited By ~ 56

Author(s):

S R Dickson ◽

M J Warburton

Keyword(s):

Mammary Gland ◽

Basement Membrane ◽

Gelatin Zymography ◽

Mammary Glands ◽

Myoepithelial Cells ◽

Immunofluorescence Staining ◽

Rat Mammary Gland

During the involution of the mammary gland there is destruction of the basement membrane as the secretory alveolar structures degenerate. Immunofluorescence staining of sections of rat mammary gland with antibodies to 72 KD gelatinase (MMP-2) and stromelysin (MMP-3) revealed increased production of these two proteinases during involution. This increased expression was mostly restricted to myoepithelial cells. Increased expression during involution was also demonstrated by immunoblotting techniques. Gelatin zymography indicated that the predominant metalloproteinase present in involuting rat mammary glands was a 66 KD gelatinase.

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Monoclonal immunoglobulin G deposits on tubular basement membrane in renal allograft: is this significant for chronic allograft injury?

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfy256 ◽

2018 ◽

Vol 34 (4) ◽

pp. 711-717

Author(s):

Anri Sawada ◽

Kunio Kawanishi ◽

Shigeru Horita ◽

Kazuya Omoto ◽

Masayoshi Okumi ◽

...

Keyword(s):

Basement Membrane ◽

Immunoglobulin G ◽

Renal Allograft ◽

Tubular Basement Membrane ◽

Monoclonal Immunoglobulin

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Antiglomerular basement membrane disease

10.1093/med/9780199592548.003.0071 ◽

2015 ◽

Author(s):

Zhao Cui ◽

Neil Turner ◽

Ming-hui Zhao

Keyword(s):

Basement Membrane ◽

Lung Disease ◽

Glomerular Basement Membrane ◽

Immunoglobulin G ◽

Acute Disease ◽

Systemic Symptoms ◽

Lung Haemorrhage

Antiglomerular basement membrane disease is characteristically the most rapidly progressive (crescentic) nephritis. It is often accompanied by lung haemorrhage, and occasionally causes lung disease alone. Its hallmark is linear deposition of immunoglobulin G along the glomerular basement membrane. There are usually few systemic symptoms apart from any related to the lung disease. Urine shows haematuria, often macroscopic in very acute disease.

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Polyclonal immunoglobulin G deposition on the tubular basement membrane in a diabetic nephropathy: A case report

Pathology International ◽

10.1111/pin.12940 ◽

2020 ◽

Vol 70 (7) ◽

pp. 463-469

Author(s):

Hirofumi Watanabe ◽

Yoichi Takeuchi ◽

Shinji Taniuchi ◽

Hiroshi Sato ◽

Yasuhiro Nakamura ◽

...

Keyword(s):

Diabetic Nephropathy ◽

Case Report ◽

Basement Membrane ◽

Immunoglobulin G ◽

Tubular Basement Membrane ◽

Polyclonal Immunoglobulin

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Intercellular deposits of basement membrane material in active human pituitary adenomas detected by immunostaining for laminin and electron microscopy

Acta Neuropathologica ◽

10.1007/bf00688057 ◽

1986 ◽

Vol 71 (3-4) ◽

pp. 326-331 ◽

Cited By ~ 5

Author(s):

S. Holck ◽

U. M. Wewer ◽

R. Albrechtsen

Keyword(s):

Electron Microscopy ◽

Basement Membrane ◽

Pituitary Adenomas ◽

Membrane Material ◽

Human Pituitary ◽

Human Pituitary Adenomas

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Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.

Journal of Histochemistry & Cytochemistry ◽

10.1177/34.8.2426332 ◽

1986 ◽

Vol 34 (8) ◽

pp. 1037-1046 ◽

Cited By ~ 99

Author(s):

A Sonnenberg ◽

H Daams ◽

M A Van der Valk ◽

J Hilkens ◽

J Hilgers

Keyword(s):

Monoclonal Antibodies ◽

Mammary Gland ◽

Epithelial Cells ◽

Basement Membrane ◽

Cell Types ◽

Mouse Mammary Gland ◽

Type I ◽

Basal Cells ◽

Alveolar Cells ◽

Luminal Type

The development of the mouse mammary gland was studied immunohistochemically using monoclonal antibodies against cell surface and basement membrane proteins and a polyclonal antibody against keratin. We have identified three basic cell types: basal, myoepithelial, and epithelial cells. The epithelial cells can be subdivided into three immunologically related cell types: luminal type I, luminal type II, and alveolar cells. These five cell types appear at different stages of mammary gland development and have either acquired or lost one of the antibody-defined antigens. The cytoplasmic distribution of several of these antigens varied according to the location of the cells within the mammary gland. Epithelial cells which did not line the lumen expressed antigens throughout the cytoplasm. These antigens were demonstrated on the apical site in situations where the cells lined the lumen. One antigen became increasingly basolateral as the cells became attached to the basement membrane. The basal cells synthesize laminin and deposit it at the cell base. They are present in endbuds and ducts and are probably the stem cells of the mammary gland. Transitional forms have been demonstrated which developmentally link these cells with both myoepithelial and (luminal) epithelial cells.

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