Age-associated Changes of Interleukin-1 Receptors on Bone Marrow Macrophages in ICR Mouse and Wistar Rat

A stromal cell line, GY30, was cloned from mouse bone marrow adherent cell layers. In culture, GY30 cells sustain the production of granulocyte-macrophage progenitor cells (GM-CFU) but fail to support the survival of pluripotential stem cells (CFU-S). GY30 cells secrete two growth factor activities distinct from interleukin-3 (IL-3), IL-2, and macrophage colony-stimulating factor (M-CSF) but functionally similar to GM-CSF and G-CSF. The production of both CSFs is increased 70- to 200-fold by treating GY30 cells with lipopolysaccharide or IL-1. RNA blot analysis reveals the presence of GM-CSF and G-CSF transcripts and demonstrates that IL-1 regulates the production of both factors at the mRNA level. Further, these studies show that the GM-CSF secreted by GY30 cells is structurally similar to the GM-CSF produced by activated T cells.

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Production of interleukin-1 by bone marrow myeloma cells

Blood ◽

10.1182/blood.v74.1.380.bloodjournal741380 ◽

1989 ◽

Vol 74 (1) ◽

pp. 380-387 ◽

Cited By ~ 1

Author(s):

F Cozzolino ◽

M Torcia ◽

D Aldinucci ◽

A Rubartelli ◽

A Miliani ◽

...

Keyword(s):

Bone Marrow ◽

Plasma Cell ◽

Plasma Cells ◽

Interleukin 1 ◽

Long Bone ◽

Bone Lesions ◽

Normal Donor ◽

Biologic Activity ◽

Culture Supernatants

Plasma cells isolated from bone marrow (BM) aspirates of 12 patients with multiple myeloma (MM) and nine patients with monoclonal gammopathy of undetermined significance (MGUS) were analyzed for production of cytokines with bone-resorbing activity, such as interleukin-1 (IL-1), tumor necrosis factor (TNF), and lymphotoxin (LT). Culture supernatants of plasma cells from MM, but not from MGUS or normal donor, invariably contained high amounts of IL-1-beta and lower amounts of IL-1-alpha. With a single exception, TNF/LT biologic activity was not detected in the same supernatants. IL-6 was present in two of five supernatants tested. Normal B lymphocytes released both IL-1 and TNF/LT activities for four days after activation in vitro; however, production of these cytokines ceased at the final stage of plasma cell. Unexpectedly, the mRNA extracted from MM plasma cell hybridized with TNF- and LT- specific, as well as IL-1-specific probes, although the culture supernatants did not contain detectable TNF/LT biologic activity. When tested in the fetal rat long bone assay, MM plasma cell supernatants displayed a strong osteoclast-activating factor (OAF) activity, which was greatly reduced but not completely abolished by neutralizing anti- IL-1 antibodies. Anti-TNF or anti-LT antibodies were ineffective in the same test. We conclude that the IL-1 released in vivo by malignant plasma cells has a major role in pathogenesis of lytic bone lesions of human MM.

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Role for interleukin-1 (IL-1) in benzene-induced hematotoxicity: inhibition of conversion of pre-IL-1 alpha to mature cytokine in murine macrophages by hydroquinone and prevention of benzene-induced hematotoxicity in mice by IL-1 alpha

Blood ◽

10.1182/blood.v78.4.938.bloodjournal784938 ◽

1991 ◽

Vol 78 (4) ◽

pp. 938-944

Author(s):

JF Renz ◽

GF Kalf

Keyword(s):

Bone Marrow ◽

Aplastic Anemia ◽

Chronic Exposure ◽

Interleukin 1 ◽

Severe Depression ◽

Genotoxic Effects ◽

Cell Lysate ◽

Dependent Inhibition ◽

Dna And Protein Synthesis ◽

Prostaglandin H

Chronic exposure of humans to benzene (BZ), a myelotoxin, causes aplastic anemia and acute leukemia. The stromal macrophage that produces interleukin-1 (IL-1), a cytokine essential for hematopoiesis, is a target of BZ's toxicity. Monocyte dysfunction and decreased IL-1 production have been shown to be involved in aplastic anemia in humans. Hydroquinone (HQ), a toxic bone marrow (BM) metabolite of BZ, causes time- and concentration-dependent inhibition of processing of the 34-Kd pre-interleukin-1 alpha (IL-1 alpha) to the 17-Kd mature cytokine in murine P388D1 macrophages and BM stromal macrophages, as measured by Western immunoblots of cell lysate proteins using a polyclonal rabbit antimurine IL-1 alpha antibody. HQ over a 10-fold concentration range had no effect on the lipopolysaccharide (LPS)-induced production of pre- IL-1 alpha precursor or on cell viability or DNA and protein synthesis. Stromal macrophages obtained from the femoral BM of C57Bl/6 mice exposed to BZ (600 or 800 mg/kg body weight) for 2 days were incapable of processing the 34-Kd pre-IL-1 alpha to the mature 17-Kd cytokine when stimulated in culture with LPS. Stromal macrophages from mice coadministered BZ and indomethacin, a prostaglandin H synthase (PHS) inhibitor that has been shown to prevent BZ-induced myelotoxic and genotoxic effects in mice when coadministered with benzene were able to convert the pre-IL-1 alpha to mature cytokine. Administration of recombinant murine IL-1 alpha (rMuIL-1 alpha) to mice before a dose of BZ that causes severe depression of BM cellularity completely prevents BM depression, most probably by bypassing the inability of the stromal macrophage in BZ-treated animals to process pre-IL-1 alpha to the mature cytokine.

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Comparative Study of Effect of Silver Nitrate Gel and Differentiated Dermal Precursors (BMSCs) on Third Degree Burn in Wistar Rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i1.1906 ◽

2020 ◽

Vol 11 (1) ◽

pp. 200-206

Author(s):

Balaji K ◽

Perumal Saraswathi ◽

Prabhu K ◽

Shila Samuel ◽

Melani Rajendren ◽

...

Keyword(s):

Bone Marrow ◽

Silver Nitrate ◽

Wistar Rats ◽

Burn Injury ◽

Wistar Rat ◽

Metal Plate ◽

Healing Time ◽

The Body ◽

Burn Wound ◽

Healing Wound

Skin is an ectodermal derivative that maintains internal homeostasis of the body. Any damage to the skin like burn injury internal homeostasis is lost, resulting in delayed healing. The aim is to study the histoarchitecture comparative effect of silver nitrate gel, and BMSCs (DDP) on third-degree burns in Wistar rats. A burn wound of size 2.5 cm (length) x 2.5 cm (breadth) x 6 mm (depth) was created using a preheated metal plate on flanks of Wistar rat. Every burn wound was treated with silver nitrate gel (commercially available as silverex), bone marrow differentiated dermal precursors, and monitored for 1, 7, 14, 21 days until wound healing. Wound surface area was measured and compared among groups with histological and gross observations. The healing time was faster in bone marrow differentiated dermal precursors (DDP) group compared to control. Prolonged silver nitrate gel usage heals burn wound with no infection, but silver toxicity was noted. Wound contraction is slower but steady using bone marrow differentiated dermal precursors (DDP) cell when compared to the group treated with silver nitrate gel. The data from this study help use to use bone marrow differentiated dermal precursors (DDP) cells as an alternate and effective way to treat burn wounds.

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Biosynthesis and distribution of leucocyte elastase inhibitor. Production of recombinant inhibitor.

Acta Biochimica Polonica ◽

10.18388/abp.1996_4482 ◽

1996 ◽

Vol 43 (3) ◽

pp. 497-501

Author(s):

A Kasza ◽

R Korpula-Mastalerz ◽

S Rose-John ◽

A Dubin

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Degradation Products ◽

Interleukin 1 ◽

Soluble Form ◽

Kinetic Properties ◽

Elastase Inhibitor ◽

Elastin Degradation ◽

Leucocyte Elastase ◽

Marrow Cells

The horse leucocyte elastase inhibitor (HLEI), present in neutrophils, monocytes and bone marrow cells, is apparently a cytoplasmic protein which is not released from cells even in response to stimulation with lipopolysaccharide, phorbol ester, tumour necrosis factor alpha, interleukin-1 or elastin degradation products. Although no expression of the inhibitor was detected in neutrophils, both monocytes and bone marrow cells were efficient in its synthesis. Using a new expression vector pREST5d, recombinant inhibitor was produced in a large quantity in a soluble form, with a yield of 88 mg per 10 litres of E. coli culture. A two-step purification procedure, consisting of ion-exchange chromatography and gel filtration, yielded 36 mg of the recombinant inhibitor of a purity higher than 95%, as judged by SDS/PAGE. The recombinant protein had physicochemical and kinetic properties indistinguishable from those of the natural one, including irreversible elastase inhibition with an association rate constant kass > 10(7) M-1s-1. Both proteins were eliminated from rat circulation at the same ratio, and within the first 20 min 70% of the protein was removed. Such a short half-life in the circulation suggests that local delivery of HLEI directly to lungs in the form of aerosol could be a more efficient therapeutic approach than its intravenous injection.

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Interleukin-1 and interleukin-6 production in peripheral blood and bone marrow mononuclear cell culture in multiple myeloma

Bulletin of Experimental Biology and Medicine ◽

10.1007/bf00784188 ◽

1993 ◽

Vol 115 (5) ◽

pp. 555-558

Author(s):

E. N. Klyushnenkova ◽

T. D. Lutskaya ◽

A. K. Golenkov ◽

V. V. Malaitsev

Keyword(s):

Multiple Myeloma ◽

Cell Culture ◽

Bone Marrow ◽

Mononuclear Cell ◽

Interleukin 6 ◽

Peripheral Blood ◽

Interleukin 1 ◽

Bone Marrow Mononuclear Cell ◽

Mononuclear Cell Culture

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Response of bone marrow stromal cells to adipogenic antagonists

Molecular and Cellular Biology ◽

10.1128/mcb.9.11.4587-4595.1989 ◽

1989 ◽

Vol 9 (11) ◽

pp. 4587-4595

Author(s):

J M Gimble ◽

M A Dorheim ◽

Q Cheng ◽

P Pekala ◽

S Enerback ◽

...

Keyword(s):

Bone Marrow ◽

Lipoprotein Lipase ◽

Bone Marrow Stromal Cells ◽

Adipocyte Differentiation ◽

Cell Clone ◽

Interleukin 1 ◽

Growth Factor Beta ◽

Necrosis Factor

Adipocytes constitute a major part of the bone marrow stroma in vivo and may play an active role in lymphohematopoiesis. Earlier studies had shown that the bone marrow stromal cell clone BMS2 was capable of adipocyte differentiation in vitro, in addition to its well-defined ability to support B lymphopoiesis. We now demonstrate that the process of adipogenesis in this functional bone marrow stromal cell clone can be inhibited by the cytokines interleukin-1 alpha, tumor necrosis factor, and transforming growth factor beta. Exposure of preadipocyte BMS2 cells to these agents blocked the induction of adipocyte differentiation as assessed by morphologic criteria and analysis of the neutral lipid content. Both interleukin-1 alpha and tumor necrosis factor elicited a rapid transient elevation in the steady-state mRNA levels of c-fos, c-jun, and JE. When added to differentiated adipocytes, the three cytokines continued to act as adipogenic antagonists. This was indicated by concentration- and time-dependent decreases in the activity of an adipocyte-specific enzyme, lipoprotein lipase. These changes in enzyme activity correlated directly with a decrease in steady-state levels of lipoprotein lipase mRNA. Another RNA marker of adipocyte differentiation (adipsin) was less influenced by the adipogenic antagonists. This may reflect the longer half-life of this mRNA transcript compared with those of lipoprotein lipase. Our results dramatically demonstrate that the differentiation state of bone marrow stromal cells can be modulated by exogenous factors in vitro. It is also the first report that transformation growth factor beta regulates the activity of lipoprotein lipase. These data suggest potential physiologic actions for these cytokines in vivo within the overall context of lymphohematopoiesis.

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Silk Sericin Activates Mild Immune Response and Increases Antibody Production

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2021.3206 ◽

2021 ◽

Vol 17 (12) ◽

pp. 2433-2443

Author(s):

Yuan Zhang ◽

Na Shi ◽

Lun He ◽

Shanshan Wang ◽

Xin Li ◽

...

Keyword(s):

Bone Marrow ◽

Immune Response ◽

T Cell Activation ◽

Specific Antibody ◽

Interleukin 1 ◽

Mouse Serum ◽

Enzyme Linked Immunosorbent Assay ◽

Splenocyte Proliferation ◽

Mouse Splenocytes ◽

Silk Sericin

To clarify whether nanoparticles of silk sericin (SS) and silk fibroin (SF) can induce inflammation and immune responses, we analyzed splenocyte proliferation, apoptosis and cytokine release to identify the effects of SS and SF on mouse splenocytes in vitro. We implanted mice with SS and SF through intraperitoneal, intramuscular, and subcutaneous routes to evaluate the innate and adaptive immune response to SS and SF in vivo. Cytokines in the serum and spleen were analyzed by Luminex and antibody array. Antigen-specific antibodies were evaluated by enzyme-linked immunosorbent assay (ELISA) at week 1 and 5 after implantation. Distinct cell populations in the spleen and bone marrow were analyzed by flow cytometry. SS suppressed the proliferation of splenocytes and CD11b+CD27− NK cells, induced splenocyte apoptosis, and increased interleukin-1 β (IL-1 β) and tumor necrosis factor-α (TNF-α) in the culture supernatant. SF suppressed splenocyte proliferation, induced splenocyte apoptosis, and increased the titer of TNF-α in culture supernatants. At both week 1 and 5 after implantation with SS, mouse serum interleukin-1 α (IL-1 α) and keratinocyte chemoattractant (KC) were decreased, SS-specific antibody was increased, the proportion of bone marrow CD4+ T cells was increased, and the proportion of splenic neutrophils was decreased. At week 5 after subcutaneous implantation with SF, mouse serum IL-1α, and splenic IL-6, TIMP-1, IL-4, MCP-1, IFN-γ, TCA-3, TNF-α, and IL-17 were decreased. SS was able to induce a mild immune response, as evidenced by CD4+ T cell activation, splenocyte apoptosis, and antigen-specific antibody secretion. Comparatively, SF had low immunogenicity and anti-inflammatory properties.

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