scholarly journals Identificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR Múltiple

2017 ◽  
Vol 28 (2) ◽  
pp. 411 ◽  
Author(s):  
Geraldine Marcelo M. ◽  
Raúl Rosadio A. ◽  
Ana Chero O. ◽  
Gerardo Díaz O. ◽  
Aldo Ciprian C. ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Salmonella Spp

El objetivo del presente estudio fue identificar mediante PCR múltiple la posible existencia de los serovares Salmonella Typhimurium y Enteritidis en 25 cepas de Salmonella spp previamente aisladas de cuyes e identificadas por sus características metabólicas. Mediante el análisis molecular se identificaron todas las cepas como Salmonella Typhimurium, evidenciando la amplificación de los cebadores específicos para los genes invA y fliC pertenecientes al género Salmonella y Salmonella Typhimurium, respectivamente. El presente estudio permitió establecer una metodología rápida para la identificación molecular de Salmonella Typhimurium y Enteritidis aislados de cuyes.

Antibiotic Resistance of Salmonella spp. and Listeria spp. Isolates from Traditionally Made Fresh Sausages in Greece

1998 ◽  
Vol 61 (10) ◽  
pp. 1378-1380 ◽  
Author(s):  
AMIN ABRAHIM ◽  
ANNA PAPA ◽  
NIKOLAOS SOULTOS ◽  
IOANNIS AMBROSIADIS ◽  
ANTONIS ANTONIADIS
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Antibacterial Agents ◽  
Listeria Innocua ◽  
Salmonella Spp ◽  
Listeria Welshimeri ◽  
Butcher Shops

Sixty-five samples of traditionally made fresh sausages obtained from retail shops and butcher shops in northem Greece were screened for the presence of Salmonella spp. and Listeria spp. Salmonella spp. were found in 20% of the samples tested (54% Salmonella typhimurium and 46% Salmonella enteritidis). The prevalence of Listeria spp. in the samples was 26% (12% Listeria monocytogenes, 76% Listeria innocua, and 12% Listeria welshimeri). Nine of 13 Salmonella isolates were found to be resistant to ampicillin and 4 of 13 showed intermediate sensitivity; 1 of 13 was found to be resistant to chloramphenicol and 1 of 13 to tetracycline. Two strains of Salmonella typhimurum were multiresistant (resistant to ampicillin, chloramphenicol, and norfloxacin). All Listeria isolates were sensitive to the antibacterial agents tested that are commonly used for the treatment of human listeriosis.

Salmonella spp. on Chicken Carcasses in Processing Plants in Poland

2002 ◽  
Vol 65 (9) ◽  
pp. 1475-1479 ◽  
Author(s):  
ANITA MIKOŁAJCZYK ◽  
MIECZYSŁAW RADKOWSKI
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Skin Surface ◽  
Body Cavity ◽  
Contamination Rate ◽  
Salmonella Spp ◽  
Continuous Cooling ◽  
Serological Type ◽  
Processing Plants ◽  
Positive Results

Chickens at selected points in the slaughter process and after slaughter on the dressing line in poultry plants were sampled and analyzed for Salmonella. These chickens came from the northeast part of Poland. The examinations were carried out in quarters I, II, III, and IV of 1999. All the birds were determined to be healthy by a veterinary inspection. Swab samples were taken from the cloaca after stunning and from the skin surface and body cavity of the whole bird after evisceration, after rinsing at the final rinse station but before chilling in the spin-chiller, and after cooling in the continuous cooling plant at the end of the production day. In 1999, 400 whole chickens were examined. The percentage of these 400 chickens from which Salmonella spp. were isolated was relatively high (23.75%; Salmonella-positive results were observed in 95 cases). Salmonella spp. were found after stunning in 6% of the chickens (6 of 100 samples), after evisceration in 24% (24 of 100), before cooling in 52% (52 of 100), and after cooling in 13% (13 of 100). These results show that Salmonella spp. were found more often at some processing points than at others. The lowest Salmonella spp. contamination rate (6%) for slaughter birds was found after stunning, and the highest contamination rate was found before chilling (52%). The serological types of Salmonella spp. isolated from whole chickens were Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Saintpaul, Salmonella Agona, and Salmonella Infantis. The results of these investigations indicate that Salmonella Enteritidis is the dominant serological type in infections of slaughter chickens, as it is in many countries.

scholarly journals Antimicrobial Resistance of Salmonella enteritidis and Salmonella typhimurium Isolated from Laying Hens, Table Eggs, and Humans with Respect to Antimicrobial Activity of Biosynthesized Silver Nanoparticles

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3554
Author(s):  
Rasha M. M. Abou Elez ◽  
Ibrahim Elsohaby ◽  
Nashwa El-Gazzar ◽  
Hala M. N. Tolba ◽  
Eman N. Abdelfatah ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Resistance Genes ◽  
Salmonella Enteritidis ◽  
Laying Hens ◽  
Resistance Index ◽  
Salmonella Spp ◽  
Biosynthesized Agnps

Salmonella enterica is one of the most common causes of foodborne illness worldwide. Contaminated poultry products, especially meat and eggs are the main sources of human salmonellosis. Thus, the aim of the present study was to determine prevalence, antimicrobial resistance profiles, virulence, and resistance genes of Salmonella Enteritidis (S. enteritidis) and Salmonella Typhimurium (S. Typhimurium) isolated from laying hens, table eggs, and humans, in Sharkia Governorate, Egypt. The antimicrobial activity of Biosynthesized Silver Nanoparticles (AgNPs) was also evaluated. Salmonella spp. were found in 19.3% of tested samples with laying hens having the highest isolation rate (33.1%). S. Enteritidis) (5.8%), and S. Typhimurium (2.8%) were the dominant serotypes. All isolates were ampicillin resistant (100%); however, none of the isolates were meropenem resistant. Multidrug-resistant (MDR) was detected in 83.8% of the isolates with a multiple antibiotic resistance index of 0.21 to 0.57. Most isolates (81.1%) had at least three virulence genes (sopB, stn, and hilA) and none of the isolates harbored the pefA gene; four resistance genes (blaTEM, tetA, nfsA, and nfsB) were detected in 56.8% of the examined isolates. The AgNPs biosynthesized by Aspergillus niveus exhibit an absorption peak at 420 nm with an average size of 27 nm. AgNPs had a minimum inhibitory concentration of 5 µg/mL against S. enteritidis and S. typhimurium isolates and a minimum bactericidal concentration of 6 and 8 µg/mL against S. enteritidis and S. typhimurium isolates, respectively. The bacterial growth and gene expression of S. enteritidis and S. typhimurium isolates treated with AgNPs were gradually decreased as storage time was increased. In conclusion, this study indicates that S. enteritidis and S. typhimurium isolated from laying hens, table eggs, and humans exhibits resistance to multiple antimicrobial classes. The biosynthesized AgNPs showed potential antimicrobial activity against MDR S. enteritidis and S. typhimurium isolates. However, studies to assess the antimicrobial effectiveness of the biosynthesized AgNPs in laying hen farms are warranted.

Salmonella spp. Shedding by Alberta Beef Cattle and the Detection of Salmonella spp. in Ground Beef

2002 ◽  
Vol 65 (3) ◽  
pp. 484-491 ◽  
Author(s):  
OLE SORENSEN ◽  
JOYCE VAN DONKERSGOED ◽  
MARGARET McFALL ◽  
KEN MANNINEN ◽  
GARY GENSLER ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Ground Beef ◽  
Feedlot Cattle ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Retail Outlets ◽  
Pulsed Field Electrophoresis ◽  
Recent Arrival ◽  
Salmonella Heidelberg

Breeder cows, cattle recently arrived at feedlots, and cattle about to be shipped for slaughter were tested for Salmonella spp. No Salmonella spp. were detected in fecal samples from breeding cows. Nineteen of 1,000 (1.9%) fecal samples from recently arrived feedlot cattle were positive for Salmonella spp. compared to only 2 of 1,000 (0.2%) fecal samples taken within 2 weeks of slaughter. The positive fecal samples were collected in 5 of 50 (10%) “recent arrival” pens tested and in 1 of 50 (2%) pens tested within 2 weeks of slaughter. The serotypes isolated were Salmonella Agona, Salmonella Enteritidis, Salmonella Typhimurium DT104, and Salmonella 4,5,12:i:−. Ground beef samples purchased from retail outlets throughout Alberta were processed for Salmonella spp. Thirteen of 1,002 (1.3%) samples were positive for Salmonella spp. The serotypes isolated from ground beef were Salmonella Anatum, Salmonella Heidelberg, Salmonella Montevideo, Salmonella Typhimurium, Salmonella Typhimurium var. Copenhagen, and Salmonella Rough-O:i:1,2. The antibiotic resistance and pulsed-field electrophoresis gel macrorestriction patterns of all isolates were compared.

scholarly journals Investigation of the incidence of Salmonella spp. in the diarrhoeic syndrome of young ruminants

2018 ◽  
Vol 51 (4) ◽  
pp. 288
Author(s):  
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ) ◽  
V. PETSAGA-TSIMPERI (Β. ΠΕΤΣΑΓΚΑ-ΤΣΙΜΠΕΡΗ) ◽  
P. TSAKOS (Π. ΤΣΑΚΟΣ)
Keyword(s):  
Public Health ◽  
Salmonella Typhimurium ◽  
Significant Role ◽  
High Mortality ◽  
Salmonella Enteritidis ◽  
Salmonella Spp ◽  
Pathogenic Agent ◽  
The Public ◽  
Haemorrhagic Diarrhoea

The presence of Salmonella spp. in the diarrhoeic syndrome of 24 cases of calves, 36 cases of kids and 14 cases of lambs was investigated. Salmonella was isolated as the pathogenic agent from six herds of calves and four cases of kids. The identified species was Salmonella enteritidis in three herds of calves and two cases of kids. Salmonella typhimurium was identified in two herds of calves and one case of kids. Also, Salmonella abony and Salmonella cerro were identified in one case of calves and kids, respectively. Furthermore, Shigella hyodysenteriae was isolated from two cases of diarrhoeic syndrome in lambs. All the infected animals were characterized by persistent haemorrhagic diarrhoea and high mortality. The occurrence of Salmonella enteritidis in ruminants is probably an important point of the epidemiology of Salmonella spp. and may play a significant role in the public health.

scholarly journals Identificación Molecular de Salmonella Typhimurium en Cuyes al Primer Parto mediante la Técnica de PCR Múltiple

2017 ◽  
Vol 28 (3) ◽  
pp. 679
Author(s):  
Ana Chero O. ◽  
Raúl Rosadio A. ◽  
Geraldine Marcelo M. ◽  
Gerardo Díaz O. ◽  
Ronald Jiménez A. ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Salmonella Spp

El estudio tuvo por objetivo identificar el serotipo de los aislados sospechosos de Salmonella spp de cuyes reproductoras en muestras recolectadas dentro de la semana del primer parto con el fin de detectar animales portadores de la bacteria. Los cuyes se encontraban clínicamente sanos y provenían de un criadero comercial de la zona de Pachacamac, Lima, Perú, sin registro de brote de salmonelosis desde hace cuatro años. Se evaluaron 272 muestras pareadas de hisopados rectales y vaginales usando protocolos microbiológicos estandarizados. Las cepas aisladas fueron evaluadas mediante pruebas bioquímicas y se procedió a la extracción de ADN de los aislados sospechosos de Salmonella spp. Posteriormente, estas muestras fueron analizadas mediante la técnica de PCR múltiple para detectar la presencia de los genes invA, prot6E y fliC específicos de Salmonella spp, Salmonella Enteritidis y Salmonella Typhimurium, respectivamente. Ocho animales (12 hisopados) resultaron positivos a Salmonella spp. Los 12 aislados amplificaron al gen invA (Salmonella spp), y de estos, 10 amplificaron para el gen fliC (Salmonella Typhimurium) y ninguno amplificó el gen prot6E (Salmonella Enteritidis). Los resultados confirmaron que Salmonella Typhimurium es el patógeno predominante en cuyes reproductoras al primer parto en esta granja de crianza comercial.

scholarly journals Перспектива використання фагів для профілактики та лікування сальмонельозу птиці

2019 ◽  
Vol 20 (2) ◽  
pp. 162-168
Author(s):  
I. K. Avdosjeva ◽  
V. V. Regenchuk ◽  
V. I. Dmitrotsa
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Salmonella Spp

У статті наведені результати бактеріологічного моніторингу матеріалу від різновікових груп птиці яєчних та м'ясних кросів із 11 птахогосподарств на наявність збудників бактеріальних інфекцій в період 2016-2018 рр. Найвищий відсоток ізольованих культур впродовж останніх трьох років становили: E.coli ˗ 72,83,78 та Salmonella spp. ˗ 17, 8, 12, відповідно. Встановлено, що ізольовані польові штами сальмонел від птиці при типізацїі зі специфічними сальмонельозними сироватками  віднесені до  Salmonella enteritidis, Salmonella typhimurium. Бактеріофаг БАФАСАЛЬ, виробництва «Протеон Фармацеутікалс», Польща, що містить композицію специфічних  фагів Salmonella enteritidis  та Salmonella typhimurium. викликав лізис 97,8 % культур сальмонел.

scholarly journals Resistance to antimicrobials drugs and control measures of Salmonella spp in the poultry industry

2013 ◽  
Vol 67 (1-2) ◽  
pp. 87-96 ◽  
Author(s):  
Maja Velhner ◽  
Dubravka Potkonjak ◽  
Dragica Stojanovic ◽  
Darko Mitevski ◽  
Igor Stojanov ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Enteritidis ◽  
Management Practice ◽  
Public Awareness ◽  
Vaccination Strategy ◽  
Developed Countries ◽  
Control Measures ◽  
Animal Origin ◽  
Salmonella Spp ◽  
Salmonella Infections

The worldwide prevalence of multiple resistant Salmonella spp is described. Clonally distributed Salmonella Enteritidis PT4 and Salmonella Typhimurium DT104 are among the most pathogenic strains for humans. Recently there have been reports on the prevalence of ST ?like? monophasic 4(5),12:i strains in some countries. Vaccination strategy and antimicorbial agent therapy is also briefly discussed. Products of animal origin must be safe and without the risk of antimicrobial resistance. Subsequently, the good management practice at farm level and HACCP in feed factories are required to cope with salmonella infections. Poultry producers in developed countries have been motivated to participate in salmonella control programs, because of public awareness on safe food and risks in the food chain. Export of poultry and poultry products is more successful in the regions where Salmonella Enteritidis and Salmonella Typhimurium have been eradicated.

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