scholarly journals SneakPeek snap: a painless microneedle-based pushbutton device for early fetal sex determination

2021 ◽  
Vol 7 (3) ◽  
pp. 74-78
Author(s):  
Nina Hoang ◽  
Haley Milot ◽  
Christopher Jacob
Keyword(s):  
Sex Determination ◽  
Ease Of Use ◽  
Blood Collection ◽  
Dramatic Reduction ◽  
Sample Collection ◽  
Fetal Sex ◽  
Blood Samples ◽  
Collection Device ◽  
Perceived Pain ◽  
Collection Time

The advancement of prenatal DNA technology and growing demand for early fetal sex determination have created a need for a simple and easy-to-use blood collection device that eliminates the pain and difficulty individuals encounter when utilizing traditional methods of blood collection such as venipuncture or lancet fingerstick. In this study, Gateway Genomics, the leading provider of fetal sex testing, introduces “SneakPeek Snap”, a novel microneedle-based, self-administered blood collection device that simplifies at-home blood collection for fetal sex testing. Our data confirms that, compared to lancet finger sticks, the SneakPeek Snap device provides users several advantages including significant reduction in perceived pain, greater ease of use, a shorter sample collection time, and a dramatic reduction in risk of sample contamination. Notably, blood samples collected using the Snap device were shown to be highly accurate for fetal sex determination — with an accuracy greater than 99%

scholarly journals Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1019
Author(s):  
Kyungjin Hong ◽  
Gabriella Iacovetti ◽  
Ali Rahimian ◽  
Sean Hong ◽  
Jon Epperson ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Blood Collection ◽  
Test Results ◽  
Sample Collection ◽  
Rapid Separation ◽  
Cell Counts ◽  
Collection Device ◽  
Precision Study ◽  
Clinically Significant ◽  
Blood Specimens

Blood sample collection and rapid separation—critical preanalytical steps in clinical chemistry—can be challenging in decentralized collection settings. To address this gap, the Torq™ zero delay centrifuge system includes a lightweight, hand-portable centrifuge (ZDrive™) and a disc-shaped blood collection device (ZDisc™) enabling immediate sample centrifugation at the point of collection. Here, we report results from clinical validation studies comparing performance of the Torq System with a conventional plasma separation tube (PST). Blood specimens from 134 subjects were collected and processed across three independent sites to compare ZDisc and PST performance in the assessment of 14 analytes (K, Na, Cl, Ca, BUN, creatinine, AST, ALT, ALP, total bilirubin, albumin, total protein, cholesterol, and triglycerides). A 31-subject precision study was performed to evaluate reproducibility of plasma test results from ZDiscs, and plasma quality was assessed by measuring hemolysis and blood cells from 10 subject specimens. The ZDisc successfully collected and processed samples from 134 subjects. ZDisc results agreed with reference PSTs for all 14 analytes with mean % biases well below clinically significant levels. Results were reproducible across different operators and ZDisc production lots, and plasma blood cell counts and hemolysis levels fell well below clinical acceptance thresholds. ZDiscs produce plasma samples equivalent to reference PSTs. Results support the suitability of the Torq System for remotely collecting and processing blood samples in decentralized settings.

scholarly journals Porcine reproductive and respiratory syndrome virus RNA detection in different matrices under typical storage conditions in the UK

2019 ◽  
Vol 185 (1) ◽  
pp. 21-21 ◽  
Author(s):  
Jinghui Fan ◽  
Priscilla F Gerber ◽  
Ana Cubas Atienzar ◽  
Lysan Eppink ◽  
Chong Wang ◽  
...  
Keyword(s):  
Storage Condition ◽  
Early Morning ◽  
Respiratory Syndrome Virus ◽  
Blood Collection ◽  
Practical Reasons ◽  
Sample Type ◽  
Sample Collection ◽  
Blood Samples ◽  
Fta Cards ◽  
The Uk

In the UK, approximately 40 per cent of the pig breeding herds are outdoors. To monitor their porcine reproductive and respiratory syndrome virus (PRRSV) status, blood is collected commonly from piglets around weaning. Sample collection in British outdoor pigs often occurs during the early morning hours when the piglets tend to accumulate inside sheltered areas. For practical reasons, dry cotton swabs are occasionally used for blood collection and stored at room temperature until arrival in the laboratory. Detection of PRRSV RNA is a function of viral concentration, sample type and storage condition. To evaluate a possible impact of the sampling protocol on PRRSV1 detection, experimentally spiked blood samples using three dilutions of a representative PRRSV1 strain were prepared. In addition, blood samples from pigs naturally infected with PRRSV were obtained from a PRRSV-positive British herd. Spiked blood and blood from infected pigs were used to obtain sera, dry or wet (immersed in saline) polyester or cotton swabs and FTA cards. The different samples were stored for 24 hours, 48 hours or 7 days at 4°C or 20°C and tested by a real-time reverse transcriptase PRRSV PCR assay. Under the study conditions, the best matrix was serum (96.7 per cent), followed by wet swabs (78 per cent), dry swabs (61.3 per cent) and FTA cards (51 per cent). Polyester swabs (76 per cent) showed a better performance than cotton swabs (63.3 per cent). The reduction in sensitivity obtained for swabs and FTA cards was particularly high at low viral concentrations. The results indicate that wet polyester swabs should be used whenever possible.

scholarly journals Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 1385 ◽  
Author(s):  
Darawan Rinchai ◽  
Esperanza Anguiano ◽  
Phuong Nguyen ◽  
Damien Chaussabel
Keyword(s):  
Human Subjects ◽  
Standard Operating Procedure ◽  
Blood Collection ◽  
Clinical Settings ◽  
Sample Collection ◽  
Individual Subject ◽  
Blood Samples ◽  
Rna Stabilization ◽  
Finger Stick ◽  
Blood Volumes

With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger-stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.

Rapid Radioimmunoassay For Fibrinopeptide A In Human Plasma

1981 ◽  
Author(s):  
B J Woodhams ◽  
P B A Kernoff
Keyword(s):  
Human Plasma ◽  
Degradation Products ◽  
Clinical Samples ◽  
Blood Collection ◽  
Sample Collection ◽  
Normal Range ◽  
Blood Samples ◽  
Fibrin Degradation Products ◽  
Sequential Samples

The originally-described method for assay of fibrino- peptide A (FPA) in human plasma includes alcohol precipitation and dialysis steps which are complex, time consuming, and limit the applicability of the assay. The purpose of this work was to devise an assay for FPA which could be more easily applied to clinical studies, and to use this assay to study some of the factors which might cause artefactual results on blood samples obtained from patients. A rapid method for FPA assay has been developed which is simple, robust and sensitive and allows results to be obtained within 2.5 hrs. of blood collection. The assay depends on the use of bentonite to absorb fibrinogen from plasma, and gives a normal range for plasma FPA (0.3 - 1.5 pmol/ml) which is similar to that measured using the originally-described method. There is an equally good correlation between results obtained by the two methods on samples obtained from patients with various levels of circulating FPA and/or fibrinogen/fibrin degradation products (FDPs). Following venepuncture, FPA levels in sequential samples of blood drawn through 19-gauge ‘Butterfly’ needles became progressively lower in successive samples, indicating that a larger than usual discard of blood is necessary if basal levels are to be accurately assessed. Different antisera showed differences in affinity for FPA and cross reaction with des amino tyrosyl FPA, but such differences are unlikely to cause problems when assaying clinical samples. Generation of FPA from whole blood in vitro is completely suppressed by heparin/Trasylol anticoagulant in conventionally-used concentration, and we therefore find no evidence in favour of the suggestion that this anticoagulant mixture is unsuitable for sample collection for FPA assay.

Influence of sampling time in the assessment of anaerobic threshold in horses

2012 ◽  
Vol 8 (2) ◽  
pp. 107-112
Author(s):  
P. Baragli ◽  
V. Vitale ◽  
M. Sgorbini ◽  
C. Sighieri
Keyword(s):  
Anaerobic Threshold ◽  
Lactate Concentration ◽  
Blood Lactate Concentration ◽  
Time Frame ◽  
Sampling Time ◽  
Blood Collection ◽  
Blood Samples ◽  
Mean Values ◽  
Collection Device ◽  
Plasma Lactate Concentration

Validity and reproducibility of anaerobic threshold (VLA4) is still matter for debate. Factors influencing blood lactate concentration, including blood collection procedure, are critical. This study aimed to evaluate influence of blood sampling times on VLA4 computing in two different horse breeds. Five Standardbreds and six Haflingers were included in this study. All the horses performed a standardised exercise test on treadmill (SET). An automatic collection device was employed to obtain blood samples every 60 seconds, in order to standardise sampling time. VLA4 was computed using the lactate data at the end of each step of the SET, and the corresponding velocity (VLA40min). The detection was then repeated for the concentrations at 1 (VLA41min), 2 (VLA42min) and 3 min (VLA43min) after the end of the 3rd step maintaining constant plasma lactate concentration of the first and the second step. VLA4 resulted increased with the VLA40min, while with the VLA41min, VLA42min and VLA43min the value of the VLA4 decreased progressively. Difference, expressed as a percentage, between VLA40min and VLA43min mean values was 16.8 and 16.6%, for Standardbred and Haflinger horses, respectively. Hence, blood samples drawn within a time frame of 3 min after the end of the SET seem to induce changes when computing of VLA4. The results suggest to carefully pay attention in standardise sampling time, collecting blood in a time frame of two minutes, one minute after the end of exercise.

scholarly journals Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes

F1000Research ◽  
2017 ◽  
Vol 5 ◽  
pp. 1385
Author(s):  
Darawan Rinchai ◽  
Esperanza Anguiano ◽  
Phuong Nguyen ◽  
Damien Chaussabel
Keyword(s):  
Human Subjects ◽  
Standard Operating Procedure ◽  
Blood Collection ◽  
Clinical Settings ◽  
Sample Collection ◽  
Individual Subject ◽  
Blood Samples ◽  
Rna Stabilization ◽  
Finger Stick ◽  
Blood Volumes

With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.

scholarly journals Early and Accurate Sex Determination by qPCR of Y Chromosome Repetitive Sequence (YRS) In Cell-Free Fetal DNA from Maternal Plasma

2018 ◽  
Vol 3 (3) ◽  
pp. 346-356 ◽  
Author(s):  
Ditte Jacobsen ◽  
Grethe Risum Krog ◽  
Frederik Banch Clausen
Keyword(s):  
Sex Determination ◽  
Y Chromosome ◽  
Repetitive Sequence ◽  
Quantitative Polymerase Chain Reaction ◽  
First Trimester ◽  
Maternal Plasma ◽  
Fetal Sex ◽  
Blood Samples ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Abstract Background Circulating cell-free fetal DNA (cffDNA) provides the opportunity for noninvasive prenatal diagnosis. Early knowledge of the fetal sex is essential in cases with a risk of a sex-linked genetic disease. A reliable and highly sensitive sex determination test is required for first trimester testing because of the low amounts of cffDNA. Methods First trimester blood samples from 326 pregnant women were analyzed by real-time quantitative polymerase chain reaction (qPCR) for the presence of Y chromosome repetitive sequence (YRS). Blood samples were collected from gestational weeks 4–12. Fetal sex was predicted on the basis of results from the YRS assay of cffDNA extracted from maternal plasma. The predicted sex was compared with the phenotypic sex of the newborn baby (n = 294). Results There was high concordance between the test results from the YRS assay and the actual sex at birth. There were no false-positive results, indicating agreement between male YRS results and male sex at birth. Two results were false negative (from gestational weeks 4 and 6) predicting female fetuses, when the actual sex at birth was male. Overall, the sensitivity of the YRS assay was 98.6% (95% CI, 95.1%–99.8%), specificity was 100% (95% CI, 97.5%–100%), and accuracy was 99.3% (95% CI, 97.5%–99.9%). From 7 weeks of gestation, sensitivity, specificity, and accuracy were 100%. Conclusions This study shows that qPCR can be used to detect and quantify repetitive DNA sequences from 0.3 genome equivalents per milliliter of plasma. Prenatal sex determination by qPCR of YRS in cffDNA from maternal plasma was reliable and robust with cffDNA extracted from 1 mL of nonhemolyzed plasma, with a plasma equivalent per PCR of 167 μL. The YRS assay can be used for early noninvasive prenatal sex determination from a gestational age of 7 weeks.

Microcomputer monitor and blood sampler for free-diving Weddell seals

1986 ◽  
Vol 61 (4) ◽  
pp. 1570-1576 ◽  
Author(s):  
R. D. Hill
Keyword(s):  
Sample Collection ◽  
Time Intervals ◽  
Laboratory Computer ◽  
Roller Pump ◽  
Blood Samples ◽  
Weddell Seals ◽  
Arterial Blood ◽  
Swimming Depth ◽  
Collection Device ◽  
Multiple Sample

The equipment used for the first sampling of arterial blood at depth on free-diving Weddell seals Leptonychotes weddelli is described. Blood was withdrawn through an aortic catheter by a submersible, peristaltic roller pump and stored in a single- or multiple-sample collection device. The multiple sampler allowed up to eight individual blood samples to be collected during a single dive. The blood pump was controlled by a dedicated microcomputer that allowed initiation of blood sampling at flexible combinations of depth and/or time during either the descending or ascending phase of the dive. The dedicated microcomputer also recorded swimming depth, velocity, heart rate, and body temperature at selectable time intervals. These data were transmitted to a laboratory computer, and blood samples were retrieved, when the seal surfaced to breathe.

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