Correlation of the Blood Test Results Obtained between Assays Using Microliter-scale Fingertip Blood Samples Collected with a Novel Blood Collection Device and Conventional Venous Blood Assays: a Secondary Publication in English

Objectives The aim of this study was to compare viscoelastic test results from samples collected from a jugular vein using a 20 G needle and a medial saphenous vein using a 22 G needle in cats presenting for elective ovariohysterectomy (OHE) or castration. Methods Forty apparently healthy cats (20 males and 20 females) presenting for elective OHE or castration were included in a prospective study observing viscoelastic test results from central and peripherally collected whole blood. Cats were anesthetized during blood collection with a standardized protocol including buprenorphine, ketamine, dexmedetomidine and isoflurane. Blood samples from jugular and saphenous veins were collected near simultaneously. Viscoelastic evaluations of whole blood were performed using a point-of-care device measuring clot time (CT), clot formation time (CFT), alpha angle (α), maximum clot formation (MCF), and amplitude at 10 and 20 mins (A10 and A20, respectively). Viscoelastometry continued post-clot time to determine a lysis index at 30 and 45 mins (LI30 and LI45, respectively) to assess fibrinolysis. Results Studied cats had a median age of 18 months (range 5 months to 5 years) and a median weight of 3.6 kg (range 2.7–5.9 kg). A total of 80 samples were available for analysis. While lysis indices were not different, viscoelastic measures of coagulation differed between sampling sites (CT, P <0.005; CFT, P = 0.01; α, P <0.05; MCF, P <0.0005; A10, P <0.0005; A20, P <0.0005). Conclusions and relevance Viscoelastic results from jugular venous blood samples appear to be more hypercoagulable than those collected from the medial saphenous vein, suggesting that the same site should be used consistently for serial monitoring or for collecting study data.

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Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization

Diagnostics ◽

10.3390/diagnostics11061019 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1019

Author(s):

Kyungjin Hong ◽

Gabriella Iacovetti ◽

Ali Rahimian ◽

Sean Hong ◽

Jon Epperson ◽

...

Keyword(s):

Clinical Chemistry ◽

Blood Collection ◽

Test Results ◽

Sample Collection ◽

Rapid Separation ◽

Cell Counts ◽

Collection Device ◽

Precision Study ◽

Clinically Significant ◽

Blood Specimens

Blood sample collection and rapid separation—critical preanalytical steps in clinical chemistry—can be challenging in decentralized collection settings. To address this gap, the Torq™ zero delay centrifuge system includes a lightweight, hand-portable centrifuge (ZDrive™) and a disc-shaped blood collection device (ZDisc™) enabling immediate sample centrifugation at the point of collection. Here, we report results from clinical validation studies comparing performance of the Torq System with a conventional plasma separation tube (PST). Blood specimens from 134 subjects were collected and processed across three independent sites to compare ZDisc and PST performance in the assessment of 14 analytes (K, Na, Cl, Ca, BUN, creatinine, AST, ALT, ALP, total bilirubin, albumin, total protein, cholesterol, and triglycerides). A 31-subject precision study was performed to evaluate reproducibility of plasma test results from ZDiscs, and plasma quality was assessed by measuring hemolysis and blood cells from 10 subject specimens. The ZDisc successfully collected and processed samples from 134 subjects. ZDisc results agreed with reference PSTs for all 14 analytes with mean % biases well below clinically significant levels. Results were reproducible across different operators and ZDisc production lots, and plasma blood cell counts and hemolysis levels fell well below clinical acceptance thresholds. ZDiscs produce plasma samples equivalent to reference PSTs. Results support the suitability of the Torq System for remotely collecting and processing blood samples in decentralized settings.

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EXTRACTION AND QUANTIFICATION OF CREATININE FROM WHOLE BLOOD SAMPLES COLLECTED WITH THE CREATININE-BCD AT HOME BLOOD COLLECTION DEVICE.

Transplantation Journal ◽

10.1097/00007890-199904150-00466 ◽

1999 ◽

Vol 67 (7) ◽

pp. S116

Author(s):

L Smith ◽

R Buelow ◽

L Carlson ◽

R Garelick ◽

J Regan

Keyword(s):

Whole Blood ◽

Blood Collection ◽

Blood Samples ◽

Collection Device ◽

Whole Blood Samples ◽

At Home

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Accuracy and Precision of a Point-of-Care HbA1c Test

Journal of Diabetes Science and Technology ◽

10.1177/1932296819831292 ◽

2019 ◽

Vol 14 (5) ◽

pp. 883-889

Author(s):

William D. Arnold ◽

Kenneth Kupfer ◽

Randie R. Little ◽

Meera Amar ◽

Barry Horowitz ◽

...

Keyword(s):

Whole Blood ◽

Point Of Care ◽

Venous Blood ◽

Reference Laboratory ◽

Test Results ◽

Blood Samples ◽

Accuracy And Precision ◽

The Mean ◽

Whole Blood Samples ◽

Highly Correlated

Background: Point-of-care (POC) hemoglobin A1c (HbA1c) testing has advantages over laboratory testing, but some questions have remained regarding the accuracy and precision of these methods. The accuracy and the precision of the POC Afinion™ HbA1c Dx test were investigated. Methods: Samples spanning the assay range were collected from prospectively enrolled subjects at three clinical sites. The accuracy of the POC test using fingerstick and venous whole blood samples was estimated via correlation and bias with respect to values obtained by an NGSP secondary reference laboratory (SRL). The precision of the POC test using fingerstick samples was estimated from duplicate results by calculating the coefficient of variation (CV) and standard deviation (SD), and separated into its components using analysis of variance (ANOVA). The precision of the POC test using venous blood was evaluated from samples run in four replicates on each of three test cartridge lots, twice per day for 10 consecutive days. The SD and CV by study site and overall were calculated. Results: Across the assay range, POC test results from fingerstick and venous whole blood samples were highly correlated with results from the NGSP SRL ( r = .99). The mean bias was −0.021% HbA1c (−0.346% relative) using fingerstick samples and −0.005% HbA1c (−0.093% relative) using venous samples. Imprecision ranged from 0.62% to 1.93% CV for fingerstick samples and 1.11% to 1.69% CV for venous samples. Conclusions: The results indicate that the POC test evaluated here is accurate and precise using both fingerstick and venous whole blood.

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Self-sampling of capillary blood for serological testing of SARS-CoV-2 by COVID-19 IgG ELISA

10.1101/2020.09.25.20183459 ◽

2020 ◽

Author(s):

Lottie Brown ◽

Rachel Louise Byrne ◽

Alice Fraser ◽

Sophie I Owen ◽

Ana I Cubas Atienzar ◽

...

Keyword(s):

Large Scale ◽

Venous Blood ◽

Dried Blood Spots ◽

Capillary Blood ◽

Blood Collection ◽

Serological Testing ◽

Perfect Agreement ◽

Blood Samples ◽

Large Scale Testing ◽

Feasible Alternative

Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty seven out of thirty eight participants were able to self-collect an adequate sample of capillary blood (≥50 μl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen′s kappa coefficient of >0.88 (near-perfect agreement). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.

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Self-sampling of capillary blood for SARS-CoV-2 serology

Scientific Reports ◽

10.1038/s41598-021-86008-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Lottie Brown ◽

Rachel L. Byrne ◽

Alice Fraser ◽

Sophie I. Owen ◽

Ana I. Cubas-Atienzar ◽

...

Keyword(s):

Large Scale ◽

Venous Blood ◽

Dried Blood Spots ◽

Capillary Blood ◽

Blood Collection ◽

Perfect Agreement ◽

Blood Samples ◽

Large Scale Testing ◽

Feasible Alternative ◽

Lateral Flow Tests

AbstractSerological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture, and the limited sensitivity of lateral flow tests. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty eight out of thirty nine participants were able to self-collect an adequate sample of capillary blood (≥ 50 µl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen’s kappa coefficient of > 0.88 (near-perfect agreement, 95% CI 0.738–1.000). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.

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Self-collection of capillary blood using Tasso-SST devices for Anti-SARS-CoV-2 IgG antibody testing

PLoS ONE ◽

10.1371/journal.pone.0255841 ◽

2021 ◽

Vol 16 (9) ◽

pp. e0255841

Author(s):

Tess Hendelman ◽

Anu Chaudhary ◽

Angela C. LeClair ◽

Kimberly van Leuven ◽

Jacqueline Chee ◽

...

Keyword(s):

Clinical Laboratory ◽

Venous Blood ◽

Epidemiologic Studies ◽

Capillary Blood ◽

Blood Collection ◽

Igg Antibodies ◽

Antibody Testing ◽

Igg Antibody ◽

Blood Samples ◽

Deming Regression

Background Efforts to minimize COVID-19 exposure during the current SARS-CoV-2 pandemic have led to limitations in access to medical care and testing. The Tasso-SST kit includes all of the components necessary for remote, capillary blood self-collection. In this study, we sought to investigate the accuracy and reliability of the Tasso-SST device as a self-collection device for measurement of SARS-CoV-2 IgG antibodies. Methods Capillary blood was obtained via unsupervised and supervised application of the Tasso-SST device, and venous blood was collected by standard venipuncture. Unsupervised self-collected blood samples underwent either extreme summer or winter-simulated shipping conditions prior to testing. Sera obtained by all three methods were tested concurrently using the EuroImmun anti-SARS-CoV-2 S1 IgG assay in a CLIA-certified clinical laboratory. Results Successful Tasso-SST capillary blood collection by unsupervised and supervised administration was completed by 93.4% and 94.5% of participants, respectively. Sera from 56 participants, 55 with documented (PCR+) COVID-19, and 33 healthy controls were then tested for anti-SARS-CoV-2 IgG antibodies. Compared to venous blood results, Tasso-SST-collected (unstressed) and the summer- and winter-stressed blood samples demonstrated Deming regression slopes of 1.00 (95% CI: 0.99–1.02), 1.00 (95% CI: 0.98–1.01), and 0.99 (95% CI: 0.97–1.01), respectively, with an overall accuracy of 98.9%. Conclusions Capillary blood self-collection using the Tasso-SST device had a high success rate. Moreover, excellent concordance was found for anti-SARS-CoV-2 IgG results between Tasso-SST capillary and standard venous blood-derived sera. The Tasso-SST device should enable widespread collection of capillary blood for testing without medical supervision, facilitating epidemiologic studies.

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Potential of Dried Blood Self-Sampling for CyclosporineC2Monitoring in Transplant Outpatients

Journal of Transplantation ◽

10.1155/2010/201918 ◽

2010 ◽

Vol 2010 ◽

pp. 1-6 ◽

Cited By ~ 15

Author(s):

Alexander Benedikt Leichtle ◽

Uta Ceglarek ◽

Helmut Witzigmann ◽

Gábor Gäbel ◽

Joachim Thiery ◽

...

Keyword(s):

Therapeutic Drug Monitoring ◽

Venous Blood ◽

Dried Blood Spots ◽

Capillary Blood ◽

Blood Sampling ◽

Therapeutic Drug ◽

Blood Collection ◽

Blood Samples ◽

Drug Levels ◽

Finger Prick

Background. Close therapeutic drug monitoring of Cyclosporine (CsA) in transplant outpatients is a favourable procedure to maintain the long-term blood drug levels within their respective narrow therapeutic ranges. Compared to basal levels (C0), CsA peak levels (C2) are more predictive for transplant rejection. However, the application ofC2levels is hampered by the precise time of blood sampling and the need of qualified personnel. Therefore, we evaluated a newC2self-obtained blood sampling in transplant outpatients using dried capillary and venous blood samples and compared the CsA levels, stability, and clinical practicability of the different procedures.Methods. 55 solid organ transplant recipients were instructed to use single-handed sampling of each 50μL capillary blood and dried blood spots by finger prick using standard finger prick devices. We used standardized EDTA-coated capillary blood collection systems and standardized filter paper WS 903. CsA was determined by LC-MS/MS. The patients and technicians also answered a questionnaire on the procedure and sample quality.Results. TheC0andC2levels from capillary blood collection systems (C0[ng/mL]:114.5±44.5;C2:578.2±222.2) and capillary dried blood (C0[ng/mL]:175.4±137.7;C2:743.1±368.1) significantly(P<.01)correlated with the drug levels of the venous blood samples (C0[ng/mL]:97.8±37.4;C2:511.2±201.5). The correlation atC0wasρcap.-ven.= 0.749, andρdried blood-ven= 0.432; atC2: ρcap.-ven.= 0.861 andρdried blood-ven= 0.711. The patients preferred the dried blood sampling because of the more simple and less painful procedure. Additionally, the sample quality of self-obtained dried blood spots for LC-MS/MS analytics was superior to the respective capillary blood samples.Conclusions.C2self-obtained dried blood sampling can easily be performed by transplant outpatients and is therefore suitable and cost-effective for close therapeutic drug monitoring.

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Influence of sampling time in the assessment of anaerobic threshold in horses

Comparative Exercise Physiology ◽

10.3920/cep12010 ◽

2012 ◽

Vol 8 (2) ◽

pp. 107-112

Author(s):

P. Baragli ◽

V. Vitale ◽

M. Sgorbini ◽

C. Sighieri

Keyword(s):

Anaerobic Threshold ◽

Lactate Concentration ◽

Blood Lactate Concentration ◽

Time Frame ◽

Sampling Time ◽

Blood Collection ◽

Blood Samples ◽

Mean Values ◽

Collection Device ◽

Plasma Lactate Concentration

Validity and reproducibility of anaerobic threshold (VLA4) is still matter for debate. Factors influencing blood lactate concentration, including blood collection procedure, are critical. This study aimed to evaluate influence of blood sampling times on VLA4 computing in two different horse breeds. Five Standardbreds and six Haflingers were included in this study. All the horses performed a standardised exercise test on treadmill (SET). An automatic collection device was employed to obtain blood samples every 60 seconds, in order to standardise sampling time. VLA4 was computed using the lactate data at the end of each step of the SET, and the corresponding velocity (VLA40min). The detection was then repeated for the concentrations at 1 (VLA41min), 2 (VLA42min) and 3 min (VLA43min) after the end of the 3rd step maintaining constant plasma lactate concentration of the first and the second step. VLA4 resulted increased with the VLA40min, while with the VLA41min, VLA42min and VLA43min the value of the VLA4 decreased progressively. Difference, expressed as a percentage, between VLA40min and VLA43min mean values was 16.8 and 16.6%, for Standardbred and Haflinger horses, respectively. Hence, blood samples drawn within a time frame of 3 min after the end of the SET seem to induce changes when computing of VLA4. The results suggest to carefully pay attention in standardise sampling time, collecting blood in a time frame of two minutes, one minute after the end of exercise.

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ВІДМІННОСТІ СКЛАДУ КРОВІ ПЕРИФЕРИЧНИХ І ЦЕНТРАЛЬНИХ ВЕН У СВИНЕЙ

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet7031 ◽

2016 ◽

Vol 18 (3(70)) ◽

pp. 132-136

Author(s):

D.V. Kibkalo ◽

S.B. Borovkov ◽

N.I. Korenev ◽

V.N. Borovkova ◽

Kh.A. Popova

Keyword(s):

Blood Test ◽

Preventive Measures ◽

Vena Cava ◽

Pathological Process ◽

Blood Analysis ◽

Blood Collection ◽

Blood Samples ◽

Small Vessels ◽

White Breed ◽

The Common

Among laboratory researches in animals the most widespread is the common blood test that can determine hidden changes in organs and tissues, monitor the effectiveness of therapeutic and preventive measures to predict outcome of disease. The question of the legality comparing the results of blood analysis from central veins with rates that are developed from capillary and peripheral vessels blood is studied in the article. Researches were conducted in seven pigs of ukrainian white breed in age 2–4 months. Blood was collected in the morning before feeding by puncture of ear vein, orbital sinus and cranial vena cava. From each animal were picked out 3 blood samples. For taking blood were applied the vacuum blood collection systems. In blood were determined the number of erythrocytes, leukocytes and hemoglobin by conventional methods (Kibkalo et al., 2016). From the results of leukocyte profile the major differences were found in the number of eosinophils whose content was significantly higher (r≤ 0.001) in the blood of ear veins. Should be noted that this index was much higher than the norm, which is possible associated with the pathological process. But their level in blood of the cranial vena cava and eye sinus was normal. Therefore, this question needs further study. Also found significantly lower (r ≤ 0.01) level of lymphocytes in the blood from the ear vein. In the vena cava were not found monocytes and basophils unlike eye sinus and ear veins, in the last their detected more. Based on the foregoing, it can be noted that in the blood of peripheral veins are registered larger number of granulocytes, which in future will be tissue macrophages. Neutrophils, monocytes, basophils and eosinophils have the ability to attach to the capillaries and small vessels walls. So they are providing the cellular immunity. In blood of central vessels are more lymphocytes that provides humoral immunity in the bloodstream. Perspectives of the next studies will be comparing the hematological results that are realized on automatic hematology analyzer from ear vein, eye sinus and cranial vena cava from the same piglets.

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