scholarly journals Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes

F1000Research ◽  
2017 ◽  
Vol 5 ◽  
pp. 1385
Author(s):  
Darawan Rinchai ◽  
Esperanza Anguiano ◽  
Phuong Nguyen ◽  
Damien Chaussabel
Keyword(s):  
Human Subjects ◽  
Standard Operating Procedure ◽  
Blood Collection ◽  
Clinical Settings ◽  
Sample Collection ◽  
Individual Subject ◽  
Blood Samples ◽  
Rna Stabilization ◽  
Finger Stick ◽  
Blood Volumes

With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.

scholarly journals Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 1385 ◽  
Author(s):  
Darawan Rinchai ◽  
Esperanza Anguiano ◽  
Phuong Nguyen ◽  
Damien Chaussabel
Keyword(s):  
Human Subjects ◽  
Standard Operating Procedure ◽  
Blood Collection ◽  
Clinical Settings ◽  
Sample Collection ◽  
Individual Subject ◽  
Blood Samples ◽  
Rna Stabilization ◽  
Finger Stick ◽  
Blood Volumes

With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing. It is often desirable to minimize blood volumes and employ methods that are the least invasive and can be practically implemented outside of clinical settings. Finger-stick blood samples are increasingly used for measurement of levels of pharmacological drugs and biological analytes. It is a simple and convenient procedure amenable for instance to field use or self-collection at home using a blood sample collection kit. Such methodologies should also enable the procurement of blood samples at high frequency for health or disease monitoring applications.

scholarly journals Porcine reproductive and respiratory syndrome virus RNA detection in different matrices under typical storage conditions in the UK

2019 ◽  
Vol 185 (1) ◽  
pp. 21-21 ◽  
Author(s):  
Jinghui Fan ◽  
Priscilla F Gerber ◽  
Ana Cubas Atienzar ◽  
Lysan Eppink ◽  
Chong Wang ◽  
...  
Keyword(s):  
Storage Condition ◽  
Early Morning ◽  
Respiratory Syndrome Virus ◽  
Blood Collection ◽  
Practical Reasons ◽  
Sample Type ◽  
Sample Collection ◽  
Blood Samples ◽  
Fta Cards ◽  
The Uk

In the UK, approximately 40 per cent of the pig breeding herds are outdoors. To monitor their porcine reproductive and respiratory syndrome virus (PRRSV) status, blood is collected commonly from piglets around weaning. Sample collection in British outdoor pigs often occurs during the early morning hours when the piglets tend to accumulate inside sheltered areas. For practical reasons, dry cotton swabs are occasionally used for blood collection and stored at room temperature until arrival in the laboratory. Detection of PRRSV RNA is a function of viral concentration, sample type and storage condition. To evaluate a possible impact of the sampling protocol on PRRSV1 detection, experimentally spiked blood samples using three dilutions of a representative PRRSV1 strain were prepared. In addition, blood samples from pigs naturally infected with PRRSV were obtained from a PRRSV-positive British herd. Spiked blood and blood from infected pigs were used to obtain sera, dry or wet (immersed in saline) polyester or cotton swabs and FTA cards. The different samples were stored for 24 hours, 48 hours or 7 days at 4°C or 20°C and tested by a real-time reverse transcriptase PRRSV PCR assay. Under the study conditions, the best matrix was serum (96.7 per cent), followed by wet swabs (78 per cent), dry swabs (61.3 per cent) and FTA cards (51 per cent). Polyester swabs (76 per cent) showed a better performance than cotton swabs (63.3 per cent). The reduction in sensitivity obtained for swabs and FTA cards was particularly high at low viral concentrations. The results indicate that wet polyester swabs should be used whenever possible.

scholarly journals Noninvasive Technique for estimating Blood Glucose Levels among diabetic Patients

2016 ◽  
Vol 17 (3) ◽  
pp. 248-252 ◽  
Author(s):  
MD Shylaja ◽  
Prashant A Punde ◽  
S Nubesh Khan ◽  
Ashutosh J Thorat
Keyword(s):  
Blood Glucose ◽  
Diabetic Patients ◽  
Sample Collection ◽  
Noninvasive Technique ◽  
Blood Samples ◽  
Bleeding On Probing ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Finger Stick ◽  
Glucose Estimation

ABSTRACT Aim The present study was aimed to assess the fasting and postprandial gingival crevicular blood (GCB) glucose and finger stick blood glucose measurements using a glucometer. Materials and methods A total of 30 subjects with periodontitis and positive bleeding on probing were considered. Subjects were instructed to report to the department after overnight fasting. Gingival crevicular blood samples were collected from anterior region showing bleeding on probing followed by finger stick blood sample collection. Then, the patients were instructed to take 75 gm of glucose and after 2 hours blood samples from two sites were collected similarly. Results were analyzed using unpaired t test and Pearson's correlation. Results Mean glucose levels form GCB and finger stick blood did not differ either during fasting or postprandial (p > 0.05). Significant correlation was found between GCB glucose levels and capillary finger stick blood (CFB) glucose levels during fasting(r = 0.946, p < 0.001) and postprandial (r = 0.930, p < 0.001) blood estimation. Conclusion Periodontal probing can be considered as an alternate noninvasive method of blood glucose estimation for screening of diabetes mellitus (DM). The technique described is safe, easy to perform, and helps to increase the frequency of diabetes screening in dental office. Clinical significance The GCB from probing can be a good source of blood for estimating blood glucose levels and screening for diabetes using portable glucose monitors. Also, it will be a simple and relatively inexpensive in office screening procedure for any patient suspected to have diabetes. How to cite this article Shylaja MD, Punde PA, Sam G, Khan SN, Latheef AA, Thorat AJ. Noninvasive Technique for Estimating Blood Glucose Levels among diabetic Patients. J Contemp Dent Pract 2016;17(3):248-252.

Rapid Radioimmunoassay For Fibrinopeptide A In Human Plasma

1981 ◽  
Author(s):  
B J Woodhams ◽  
P B A Kernoff
Keyword(s):  
Human Plasma ◽  
Degradation Products ◽  
Clinical Samples ◽  
Blood Collection ◽  
Sample Collection ◽  
Normal Range ◽  
Blood Samples ◽  
Fibrin Degradation Products ◽  
Sequential Samples

The originally-described method for assay of fibrino- peptide A (FPA) in human plasma includes alcohol precipitation and dialysis steps which are complex, time consuming, and limit the applicability of the assay. The purpose of this work was to devise an assay for FPA which could be more easily applied to clinical studies, and to use this assay to study some of the factors which might cause artefactual results on blood samples obtained from patients. A rapid method for FPA assay has been developed which is simple, robust and sensitive and allows results to be obtained within 2.5 hrs. of blood collection. The assay depends on the use of bentonite to absorb fibrinogen from plasma, and gives a normal range for plasma FPA (0.3 - 1.5 pmol/ml) which is similar to that measured using the originally-described method. There is an equally good correlation between results obtained by the two methods on samples obtained from patients with various levels of circulating FPA and/or fibrinogen/fibrin degradation products (FDPs). Following venepuncture, FPA levels in sequential samples of blood drawn through 19-gauge ‘Butterfly’ needles became progressively lower in successive samples, indicating that a larger than usual discard of blood is necessary if basal levels are to be accurately assessed. Different antisera showed differences in affinity for FPA and cross reaction with des amino tyrosyl FPA, but such differences are unlikely to cause problems when assaying clinical samples. Generation of FPA from whole blood in vitro is completely suppressed by heparin/Trasylol anticoagulant in conventionally-used concentration, and we therefore find no evidence in favour of the suggestion that this anticoagulant mixture is unsuitable for sample collection for FPA assay.

scholarly journals SneakPeek snap: a painless microneedle-based pushbutton device for early fetal sex determination

2021 ◽  
Vol 7 (3) ◽  
pp. 74-78
Author(s):  
Nina Hoang ◽  
Haley Milot ◽  
Christopher Jacob
Keyword(s):  
Sex Determination ◽  
Ease Of Use ◽  
Blood Collection ◽  
Dramatic Reduction ◽  
Sample Collection ◽  
Fetal Sex ◽  
Blood Samples ◽  
Collection Device ◽  
Perceived Pain ◽  
Collection Time

The advancement of prenatal DNA technology and growing demand for early fetal sex determination have created a need for a simple and easy-to-use blood collection device that eliminates the pain and difficulty individuals encounter when utilizing traditional methods of blood collection such as venipuncture or lancet fingerstick. In this study, Gateway Genomics, the leading provider of fetal sex testing, introduces “SneakPeek Snap”, a novel microneedle-based, self-administered blood collection device that simplifies at-home blood collection for fetal sex testing. Our data confirms that, compared to lancet finger sticks, the SneakPeek Snap device provides users several advantages including significant reduction in perceived pain, greater ease of use, a shorter sample collection time, and a dramatic reduction in risk of sample contamination. Notably, blood samples collected using the Snap device were shown to be highly accurate for fetal sex determination — with an accuracy greater than 99%

scholarly journals Importance of Preanalytical Factors in Measuring Cr and Co Levels in Human Whole Blood: Contamination Control, Proper Sample Collection and Long-Term Storage Stability

2020 ◽  
Author(s):  
Yuliya L Sommer ◽  
Cynthia D Ward ◽  
Joaudimir Castro Georgi ◽  
Po-Yung Cheng ◽  
Robert L Jones
Keyword(s):  
Stainless Steel ◽  
Whole Blood ◽  
Consumer Products ◽  
Blood Collection ◽  
Sample Collection ◽  
Blood Samples ◽  
Contamination Control ◽  
Human Whole Blood

Abstract A number of errors with potentially significant consequences may be introduced at various points in the analytical process, which result in skewed, erroneous analytical results. Precautionary procedures such as contamination control, following established sample collection protocols, and having a complete understanding of the long-term stability of the elements of interest can minimize or eliminate these errors. Contamination control is critical in the quantification of Cr and Co in human whole blood. Cr and Co levels in most biological samples are low, but these elements occur naturally in the environment and are often found in commercial and consumer products, which increases the risk of contamination. In this paper, we demonstrated that lot screening process in which we pre-screen a sub-set of manufactured lots used in collecting, analyzing and storing blood samples is a critical step in controlling Cr and Co contamination. Stainless steel needles are often utilized in blood collection but are considered as a potential source of introducing metal contamination to the patient sample. We conducted two studies to determine if there is a possibility of Cr or Co leaching into the human whole blood from the needles during blood collection. We analyzed blood collected from 100 donors and blood collected in vitro in the laboratory from designated vessel containing spiked blood with higher levels of Cr and Co. Two blood tubes were consecutively collected through one needle. In both studies, Cr and Co concentration levels in the two consecutively collected tubes were compared. Based on the results from donor and in vitro blood collection studies, we concluded that there was no Cr and Co leaching from the limited sets of stainless steel needles used in these studies. Furthermore, we demonstrated that Cr and Co human whole blood samples are stable for 1 year stored at temperatures of −70, −20 and 4°C and 6 months at room temperature.

scholarly journals Effect of Elapsed Time after Blood Collection on the Viability and Mitotic Index of Human Lymphocytes during Karyotype Analysis

2019 ◽  
pp. 1-9
Author(s):  
Doaa Hussein El-Khateeb ◽  
Ashraf Abd Elraouf Khalil ◽  
Ibrahim Tantawy El Sayed ◽  
Hala Hany EL-Said
Keyword(s):  
Mitotic Index ◽  
Human Lymphocytes ◽  
Venous Blood ◽  
Chromosome Analysis ◽  
Lymphocyte Culture ◽  
Objective Lens ◽  
Blood Collection ◽  
Sample Collection ◽  
Blood Samples ◽  
Chromosome Staining

Background: Chromosome staining using G banding is a commonly used technique during karyotyping, however, a limited number of laboratories carries out the test. Blood samples must be sent to the laboratory on the same day of sample collection. Aim: To assess the effect of time passed from sample withdrawal to the beginning of lymphocyte culture on lymphocyte viability and the mitotic index of chromosomal spread. Methods: Collected peripheral venous blood samples were either processed for chromosome analysis within 2h of samples collection or stored at 4°C then processed at 24h and 48h. Lymphocytes viability was determined by trypan blue and mitotic cells were visualized by the lighted microscope at the 40x objective lens. Mitotic index was calculated per 1000 cell count. Results: Delay in sample processing more than 24h have a deleterious effect on lymphocyte viability with a significant reduction in mitotic index relative to the freshly processed sample. Conclusion: Culturing of cells within 24h of sample collection is highly recommended whenever possible and delay more than 48h should be avoided.

scholarly journals Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks

2010 ◽  
Vol 5 ◽  
pp. BMI.S5522 ◽  
Author(s):  
Daniel Gilbert Weber ◽  
Swaantje Casjens ◽  
Peter Rozynek ◽  
Martin Lehnert ◽  
Sandra Zilch-Schöneweis ◽  
...  
Keyword(s):  
Human Blood ◽  
Pcr Analysis ◽  
Blood Collection ◽  
Multicenter Studies ◽  
Blood Samples ◽  
Ambient Temperatures ◽  
Analysis Laboratory ◽  
Human Blood Samples ◽  
Rna Stabilization ◽  
Blood Collection Tubes

In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies.

Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months

2018 ◽  
Vol 88 (3-4) ◽  
pp. 151-157 ◽  
Author(s):  
Scott W. Leonard ◽  
Gerd Bobe ◽  
Maret G. Traber
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Healthy Subjects ◽  
Frozen Storage ◽  
Blood Collection ◽  
Plasma Samples ◽  
Optimal Conditions ◽  
Plasma Ascorbic Acid ◽  
Blood Samples ◽  
Sample Handling

Abstract. To determine optimal conditions for blood collection during clinical trials, where sample handling logistics might preclude prompt separation of erythrocytes from plasma, healthy subjects (n=8, 6 M/2F) were recruited and non-fasting blood samples were collected into tubes containing different anticoagulants (ethylenediaminetetra-acetic acid (EDTA), Li-heparin or Na-heparin). We hypothesized that heparin, but not EDTA, would effectively protect plasma tocopherols, ascorbic acid, and vitamin E catabolites (α- and γ-CEHC) from oxidative damage. To test this hypothesis, one set of tubes was processed immediately and plasma samples were stored at −80°C, while the other set was stored at 4°C and processed the following morning (~30 hours) and analyzed, or the samples were analyzed after 6 months of storage. Plasma ascorbic acid, as measured using HPLC with electrochemical detection (LC-ECD) decreased by 75% with overnight storage using EDTA as an anticoagulant, but was unchanged when heparin was used. Neither time prior to processing, nor anticoagulant, had any significant effects upon plasma α- or γ-tocopherols or α- or γ-CEHC concentrations. α- and γ-tocopherol concentrations remained unchanged after 6 months of storage at −80°C, when measured using either LC-ECD or LC/mass spectrometry. Thus, refrigeration of whole blood at 4°C overnight does not change plasma α- or γ-tocopherol concentrations or their catabolites. Ascorbic acid is unstable in whole blood when EDTA is used as an anticoagulant, but when whole blood is collected with heparin, it can be stored overnight and subsequently processed.

1-Desamino-8-D-Arginine Vasopressin (DDAVP) Infusion in Type IIB von Willebrand’s Disease: Shortening of Bleeding Time and Induction of a Variable Pseudothrombocytopenia

1990 ◽  
Vol 64 (01) ◽  
pp. 117-120 ◽  
Author(s):  
Alessandra Casonato ◽  
M Teresa Sartori ◽  
Luigi de Marco ◽  
Antonio Girolami
Keyword(s):  
Monoclonal Antibody ◽  
Platelet Count ◽  
Arginine Vasopressin ◽  
Calcium Concentration ◽  
Sodium Citrate ◽  
Bleeding Time ◽  
Blood Collection ◽  
Von Willebrand's Disease ◽  
Blood Samples ◽  
Von Willebrand’S Disease

SummaryWe have investigated the effects of 1-desamino-8-D-arginine vasopressin (DDAVP) infusion on platelet count and bleeding time in 4 patients with type IIB von Willebrand’s disease (vWd). Three of four patients showed a normalization of the bleeding time within 1 h after the infusion, while bleeding time was not modified in the fourth. In accordance with the literature, thrombocytopenia was observed after DDAVP infusion, but this thrombocytopenia was due to the anticoagulants used for blood collection. In two patients (F. I., G. F.) no thrombocytopenia was observed when platelets were counted by fingerstick method but there was a 20% platelet decrease in blood samples collected in sodium citrate and a 50% decrease in samples collected in EDTA. Dramatic falls in platelet counts (70–95%) were observed in the additional two patients (C. A., D.Z.) after DDAVP infusion, when both sodium citrate or EDTA were used as anticoagulants. In the latter two patients there was also a 50% decrease in platelet count when the fingerstick method was used. The decrease in the patient’s platelet count in EDTA samples after DDAVP infusion could be prevented, in part, by the previous additions of an anti GPIb monoclonal antibody and an anti GPIIb-IIIa monoclonal antibody.Thus, the thrombocytopenia observed in the four IIB vWd patients studied after DDAVP infusion seems to be, at least partially, a pseudothrombocytopenia depending on the calcium concentration in the blood samples and the availability of GPIb and GPIIb-IIIa receptors. These findings and the normalization of the bleeding time observed in three of the four patients has led us to reconsider the possible use of DDAVP in the treatment of our IIB vWd patients.

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