scholarly journals Investigation of enterobacter aerogenes effects on heavy oil from biological degradation aspects by GC*GC technique

2019 ◽  
Vol 4 (2) ◽  
pp. 47-52
Author(s):  
Arash Rasti ◽  
Mahmud Memariani ◽  
Mohammad Ali Riahi
Keyword(s):  
Crude Oil ◽  
Heavy Oil ◽  
Oil Pollution ◽  
Enterobacter Aerogenes ◽  
Oil Production ◽  
Cost Effective ◽  
Biological Degradation ◽  
Emergency Situations ◽  
Oil Residue ◽  
Light Oil

The main cause of oil pollution of soil is the emergency situations within oil production, transportation and processing in the boundaries of industrial sites of chemical and petrochemical industries. Oil pollution leads to the deterioration of the agrophysics soil characteristics, namely to the dysfunction of the water, air, thermal, oxidation-reduction and nutrient regimens. Microbial technologies are becoming accepted worldwide as cost-effective and environmentally friendly approaches to improve oil production. One of the methods to purify oil residue is bioremediation, in this way we Investigate Enterobacter Aerogenes effects on heavy oil by SARA, FT-IR, GC and GCxGC methods. The material required for the growth of Enterobacter Aerogenes is Carbon and Phosphate. On the one hand, this bacterium takes these nutrients from carbohydrates in our body. In addition, the crude oil is full of Hydrocarbon structures. So, the Enterobacter Aerogenes is injected with Thioglycollate broth to crude oil which was distillated and the samples were put for a month in an incubator for 30 days. The results this research illustrate this bacterium has been able to survive in oil by isolating straight-run carbons with short and weak bounds from asphaltene and resin structures, as well as utilizing sulfur, nitrogen and oxygen in the oil. Also, it is found out this bacterium has had a positive effect on making light oil from heavy crude oil. It should be noted this bacterium releases CH4, which can be used again if the gas is collected.

scholarly journals Production of High Purity Biosurfactants Using Heavy Oil Residues as Carbon Source

Energies ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3557
Author(s):  
Athina Mandalenaki ◽  
Nicolas Kalogerakis ◽  
Eleftheria Antoniou
Keyword(s):  
Carbon Source ◽  
Heavy Oil ◽  
Oil Pollution ◽  
Batch Reactor ◽  
Production Method ◽  
Promising Alternative ◽  
Heavy Oil Residues ◽  
Oil Residue ◽  
Increasing Demand ◽  
Heavy Oil Residue

Typically, oil pollution cleanup procedures following first response actions include dispersion. Crude oil is biodegradable, and its bioavailability can be increased when dispersed into very fine droplets by means of chemical surfactants. Although their use is widely spread in many applications, the latter may prove toxic, depending on the extent of use. The use of biological means, such as bioremediation and biosurfactants, has emerged over the past years as a very promising ‘green’ alternative technology. Biosurfactants (BSs) are amphiphilic molecules produced by microorganisms during biodegradation, thus increasing the bioavailability of the organic pollutants. It is their biodegradability and low toxicity that render BSs as a very promising alternative to the synthetic ones. Alcanivorax borkumensis SK2 strain ability to produce BSs, without any impurities from the substrate, was investigated. The biosurfactant production was scaled up by means of a sequencing batch reactor (SBR) and a heavy oil residue substrate as the carbon source. The product is free from substrate impurities, and its efficiency is tested on oil bioremediation in the marine environment. The product’s dispersion efficiency was determined by the baffled flask test. The production method proposed can have a significant impact to the market, given the ever-increasing demand for ecologically friendly, reliable, commercially viable and economically competitive environmental cleanup techniques.

Cost-effective Time Lapse Seismic Based on Direct Transmitted P-waves for Monitoring Sagd Heavy Oil Production

2018 ◽  
Author(s):  
M. Gareev ◽  
M. Amerkhanov ◽  
M. Lyabipov ◽  
A. Stepanov ◽  
R. Sitdikov ◽  
...  
Keyword(s):  
Heavy Oil ◽  
Oil Production ◽  
Cost Effective ◽  
Time Lapse ◽  
P Waves ◽  
Effective Time

scholarly journals Metagenomics insight into microbial dynamics in field-scale remediation of crude oil-polluted soil

2020 ◽  
Author(s):  
Chioma Blaise Chikere ◽  
Memory Tekere ◽  
Rasheed Adeleke
Keyword(s):  
Crude Oil ◽  
Oil Spill ◽  
Niger Delta ◽  
Oil Pollution ◽  
Alpha Diversity ◽  
Cost Effective ◽  
Polluted Soil ◽  
Field Scale ◽  
Microbial Dynamics ◽  
Naphthalene Degradation

Abstract Background: The frequency of crude oil pollution has been on the increase following increased exploration, exploitation and production of energy from fossil fuel. Bioremediation has been shown to be eco-friendly and cost-effective method of oil spill remediation. In the Niger Delta, Landfarming has been the most used technique. The aim of this research was to employ metagenomic techniques to understand microbial dynamics during field-scale remediation in the Niger Delta in order to improve and reduce the time of remediation. Results: The surface (0.0 – 0.5m) sample had an extractable TPH value of 6231 mg/kg. The subsurface samples from 1m, 1.5m and 2.0m depths had extractable TPH concentration of 4836 mg/kg, 9112 mg/kg and 7273 mk/kg respectively. Proteobacteria dominated the bacterial community of the oil-polluted soil and comprised mainly of the classes Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria. Alpha diversity analysis revealed the presence of crude oil in the soil reduced microbial diversity. Principal coordinate analysis showed the microbial structure continually changed following changes in the chemical composition of the soil. Mycobacterium, Burkholderia, Methylobacterium and Bacillus were among the core OTUs detected during the period of remediation. Significant variation in pathway abundance particularly pathways for propanoate degradation, benzoate degradation, naphthalene degradation, fatty acid metabolism, polycyclic aromatic hydrocarbon degradation and degradation of xenobiotics were observed when the unpolluted soil was compared to the samples obtained during remediation. Conclusions: The findings from this study will greatly advance an already preferred landfarming oil spill recovery technique in the Niger Delta.

scholarly journals Metagenomics insight into microbial dynamics in field-scale remediation of crude oil-polluted soil

2020 ◽  
Author(s):  
Chioma Blaise Chikere ◽  
Memory Tekere ◽  
Rasheed Adeleke
Keyword(s):  
Crude Oil ◽  
Oil Spill ◽  
Niger Delta ◽  
Oil Pollution ◽  
Alpha Diversity ◽  
Cost Effective ◽  
Field Scale ◽  
Microbial Dynamics ◽  
Microbial Profile ◽  
Naphthalene Degradation

Abstract Background: The frequency of crude oil pollution has been on the increase following increased exploration, exploitation and production of energy from fossil fuel. Bioremediation has been shown to be eco-friendly and cost-effective method of oil spill remediation. In the Niger Delta, Landfarming has been the most used technique. The aim of this research was to employ metagenomic techniques to understand microbial dynamics during field-scale remediation in the Niger Delta in order to improve and reduce the time of remediation. Results: The surface (0.0 – 0.5m) sample had an extractable TPH value of 6231 mg/kg. The subsurface samples from 1m, 1.5m and 2.0m depths had extractable TPH concentration of 4836 mg/kg, 9112 mg/kg and 7273 mk/kg respectively. Proteobacteria dominated the soil microbial profile in all the samples studied as it made up at least 50% of each sample and mostly comprised of the class Alphaproteobacteria with variation only on day 18 and 36 which was mostly dominated by the class Gammaproteobacteria and Betaproteobacteria. Alpha diversity analysis revealed the presence of crude oil in the soil reduced microbial diversity. Principal coordinate analysis showed the microbial structure continually changed following changes in the chemical composition of the soil. Mycobacterium, Burkholderia, Rhodoplanes, Methylobacterium and Bacillus were the core OTUs detected during the period of remediation. Significant variation in pathway abundance particularly pathways for propanoate degradation, benzoate degradation, naphthalene degradation, fatty acid metabolism, polycyclic aromatic hydrocarbon degradation and degradation of xenobiotics were observed when the unpolluted soil was compared to the samples obtained during remediation. Conclusions: The findings from this study will greatly advance an already preferred landfarming oil spill recovery technique in the Niger Delta.

scholarly journals Heavy Oil Transportation by Pipeline

1996 ◽  
Author(s):  
John M. Gerez ◽  
Archie R. Pick
Keyword(s):  
Crude Oil ◽  
Heavy Oil ◽  
Oil Production ◽  
Annual Production ◽  
Heavy Crude Oil ◽  
Daily Production ◽  
Oil Transportation ◽  
Crude Oil Production ◽  
The World ◽  
Heavy Crude

More of the crude oil being produced in the world is heavy oil. It was reported by Meyer and Dietzman (1979) that world annual production of heavy crude oil was about five percent of other oil produced. They forecast that heavy crude oil production would increase. Canadian heavy oil production cumulative to 1979 was reported to be 197 million barrels. By 1996 Canadian daily production levels have risen to the levels shown in Table 1, with annual production of heavy oil and bitumen exceeding cumulative totals produced to 1979.

scholarly journals Aerobic degradation of oil-based mud drilling fluid by in situ bacteria in the Hawizeh Marshes

2021 ◽  
Author(s):  
Arash Rasti ◽  
Adel Ameri ◽  
Mohammad Ali Riahi
Keyword(s):  
Gas Chromatography ◽  
Crude Oil ◽  
Oil Pollution ◽  
Drilling Fluid ◽  
High Growth ◽  
Drilling Process ◽  
Biological Degradation ◽  
Chemical Additives ◽  
Degrading Bacteria

AbstractThe increasing global demand for hydrocarbon has brought new challenges in the petroleum reservoir's drilling process. Non-biodegradable chemical additives are used in drilling fluid and it leads to endangering the environment and personnel safety. Thus, there is a great need for new biodegradable drilling fluid additives that can protect the environment and personal safety while drilling is done well. This study with help of microbial analysis investigates the effects of in situ bacteria to degenerate the gasoline inside the oil-based mud, in the returned lubricant from one of the oil wells next to the Hawizeh Marshes. Four types of bacteria inside the oil-based mud were observed. According to the high growth and degradation of crude oil, one strain was selected. Determination of the coagulase and clumping test shows that the isolated strain belongs to staphylococcus. We investigate the performance of the staphylococcus bacterium on the lubricant from biological degradation aspects, using a gas chromatography technique. It was observed that the exposure of a small amount of the bacteria against 10 mL of lubricant indicates a considerable degree of degeneration, only in a few days. The residual crude oil in the culture medium was analyzed by gas chromatography (GC) and SARA. The results confirmed that the strain can degrade crude oil and produce lighter hydrocarbon. The saturate fraction increased about 23%, while the Resin and Asphaltene fractions decreased about 11% and 12%, respectively. This research is the first report on the characterization of crude oil-degrading bacteria from in situ bacteria at Hawizeh Marsh and by using this bacterium in the field the effect of oil pollution can be reduced on this marsh environment in a few days.

Creation of the ‘Internet of things’ in crude oil production

2017 ◽  
Vol 10 ◽  
pp. 120-124
Author(s):  
R.S. Khisamov ◽  
◽  
R.A. Gabdrahmanov ◽  
A.P. Bespalov ◽  
V.V. Zubarev ◽  
...  
Keyword(s):  
Internet Of Things ◽  
Crude Oil ◽  
Oil Production ◽  
The Internet ◽  
Crude Oil Production ◽  
The Internet Of Things
Sign in / Sign up

Export Citation Format

Share Document