scholarly journals Intramammary Propolis Formulation for Subclinical Mastitis Prevention and Treatment in Dairy Cows

2016 ◽  
Vol 3 (5) ◽  
Author(s):  
Goran Bacic
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Prevention And Treatment

scholarly journals Formulation and application of nanosilver-infused cream in prevention and treatment of mastitis in dairy cows

2019 ◽  
Vol 41 (2) ◽  
Author(s):  
Nguyen Hoai Chau ◽  
Dao Trong Hien ◽  
Nguyen Thi Thuy ◽  
Doan Quang Ha ◽  
Dang Thi Duong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Treatment Rate ◽  
Environmental Technology ◽  
Long Term Stability ◽  
High Uniformity ◽  
Prevention And Treatment ◽  
Mastitis Pathogens

Research on formulation of nanosilver-infused cream was carried out at the Institute of Environmental Technology and the effects of this cream in prevention and treatment of mastitis in dairy cows were assessed at Bavi Cattle and Forage Research Center. Cream with 0.4% carbomer was infused with silver nanoparticles for high uniformity and long-term stability. At concentrations of 50–250 ppm, nanosilver-infused cream proved to be effective against mastitis pathogens such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. Nanosilver cream at concentrations of 150 ppm had the best effect on clinical and subclinical mastitis prevention in dairy cows. Combining nanosilver cream with ceftiofer antibiotic achieved excellent treatment rate, up to 93.33%. For prevention of dairy mastitis, silver cream can be used at a concentration of 150 ppm 15 days before calving and for the whole milking period. Using nanosilver cream in combination with antibiotics led to highly effective mastitis treatment. 

Mass-spec-based urinary metabotyping around parturition identifies screening biomarkers for subclinical mastitis in dairy cows

2020 ◽  
Vol 129 ◽  
pp. 39-52
Author(s):  
Grzegorz Zwierzchowski ◽  
Guanshi Zhang ◽  
Rupasri Mandal ◽  
David S. Wishart ◽  
Burim N. Ametaj
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Mass Spec

Evaluation of the udder health status in subclinical mastitis affected dairy cows through bacteriological culture, somatic cell count and thermographic imaging

2015 ◽  
Vol 18 (4) ◽  
pp. 799-805 ◽  
Author(s):  
A. Bortolami ◽  
E. Fiore ◽  
M. Gianesella ◽  
M. Corrò ◽  
S. Catania ◽  
...  
Keyword(s):  
Health Status ◽  
Dairy Cows ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Subclinical Mastitis ◽  
Bacteriological Culture ◽  
Udder Health ◽  
Streptococcus Uberis ◽  
Thermographic Imaging

Abstract Subclinical mastitis in dairy cows is a big economic loss for farmers. The monitoring of subclinical mastitis is usually performed through Somatic Cell Count (SCC) in farm but there is the need of new diagnostic systems able to quickly identify cows affected by subclinical infections of the udder. The aim of this study was to evaluate the potential application of thermographic imaging compared to SCC and bacteriological culture for infection detection in cow affected by subclinical mastitis and possibly to discriminate between different pathogens. In this study we evaluated the udder health status of 98 Holstein Friesian dairy cows with high SCC in 4 farms. From each cow a sample of milk was collected from all the functional quarters and submitted to bacteriological culture, SCC and Mycoplasma spp. culture. A thermographic image was taken from each functional udder quarter and nipple. Pearson’s correlations and Analysis of Variance were performed in order to evaluate the different diagnostic techniques. The most frequent pathogen isolated was Staphylococcus aureus followed by Coagulase Negative Staphylococci (CNS), Streptococcus uberis, Streptococcus agalactiae and others. The Somatic Cell Score (SCS) was able to discriminate (p<0.05) cows positive for a pathogen from cows negative at the bacteriological culture except for cows with infection caused by CNS. Infrared thermography was correlated to SCS (p<0.05) but was not able to discriminate between positive and negative cows. Thermographic imaging seems to be promising in evaluating the inflammation status of cows affected by subclinical mastitis but seems to have a poor diagnostic value.

scholarly journals Molecular characterization of virulence factors in Staphylococcus aureus isolated from bovine subclinical mastitis in central Ethiopia

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Desiye Tesfaye Tegegne ◽  
Gezahegne Mamo ◽  
Hika Waktole ◽  
Yohannes Equar Messele
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Virulence Factors ◽  
Virulence Genes ◽  
Animal Health ◽  
Subclinical Mastitis ◽  
Biochemical Tests ◽  
Milk Samples ◽  
Lactating Dairy Cows ◽  
Genomic Study

Abstract Purpose Staphylococcus aureus (S. aureus) is the most important pathogen involved in bovine mastitis in dairy production. S. aureus produces a spectrum of extracellular protein toxins and virulence factors which are thought to contribute to the pathogenicity of the organism. The aim of this work was to isolate and molecular characterize S. aureus associated with bovine subclinical mastitis in the central part of Ethiopia. Methods A total of 265 lactating dairy cows from various dairy farms in four different geographical locations were screened by the California mastitis test (CMT) for bovine subclinical mastitis. One hundred thirty CMT-positive milk samples were collected and transported to the laboratory. Different biochemical tests and polymerase chain reaction (PCR) were used for the identification of S. aureus isolates. Finally, PCR was performed for molecular detection of virulence genes. Results From a total of 265 lactating dairy cows screened, 49% (n = 130) were positive for bovine subclinical mastitis. One hundred thirty mastitic milk samples were subjected to bacterial culturing, and one hundred (76%) S. aureus isolates were identified based on phenotypic characters. Sixty-eight confirmed S. aureus isolates were obtained using PCR. The confirmed S. aureus isolates were tested for six virulence genes (tsst-1, hlb, eta, sea, clfA, and icaD) using PCR. Of the six virulence genes screened from all the isolates, only two (clfA and eta) were detected in the isolates. Out of 68 isolates, 25% and 22% were possessed the eta and clfA genes, respectively. Conclusion The presence of Staphylococcus aureus having virulence genes (eta and clfA) revealed that mastitis is a major concern nowadays affecting animal health, milk quality, and yield. Further genomic study of these isolates will provide broad new insights on virulence.

scholarly journals Mycoplasmal mastitis in dairy cows in the Moghan region of Ardabil State, Iran : short communication

2006 ◽  
Vol 77 (4) ◽  
Author(s):  
C. Ghazaei
Keyword(s):  
Dairy Cows ◽  
Short Communication ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Mycoplasma Bovis ◽  
Bulk Tank Milk ◽  
Milk Samples ◽  
The World ◽  
Bulk Tank

Mycoplasmas are an important and economically significant cause of mastitis in dairy cows in various parts of the world. The organisms are highly contagious, with the main reservoir of infection originating from cows with subclinical mastitis. In 1998 the 1st cases of bovine mastitis due to Mycoplasma bovis were diagnosed in Ardabil State, Iran. An investigation was carried out with the aim of establishing the extent of mycoplasma infections in dairy cows in Ardabil State. Milk samples obtained from 80 cows with clinical mastitis were cultured in the laboratory for the presence of mycoplasmas. Similarly, 48 bulk-tank milk samples were examined for the presence of mycoplasmas. A modified Hayflick broth was used to isolate the mycoplasmas and an immunoperoxidase test used for the species identification of the isolates. Mycoplasma bovis was isolated from 39 (48.75 %) of the clinical mastitis samples and from 48 of the bulk-tank milk samples tested. This indicated that mycoplasma udder infections were more prevalent in dairy cows in Ardabil State than previously thought.

scholarly journals Research on subclinical mastitis and its ethiology in different breeds of cows

2015 ◽  
Vol 31 (3) ◽  
pp. 365-374
Author(s):  
N. Varatanovic ◽  
B. Cengic ◽  
E. Imsirevic
Keyword(s):  
Positive Reaction ◽  
Subclinical Mastitis ◽  
Mixed Infections ◽  
California Mastitis Test ◽  
Holstein Friesian ◽  
Common Causes ◽  
Prevention And Treatment ◽  
Quality Of Milk

Detection of subclinical mastitis in five different breeds of cows were done on the territory of a municipality Kljuc, through all four year seasons by using the California mastitis test, at the level of the udder?s quarter with a certificate of bacteriological findings in order to justify their application in the diagnosis of mastitis. In total, 2150 cows of different breed composition: Simmental 1090, red Holstein 322, Holstein-Friesian 340, montafon 108 and various crossbreeds of 290 in total. After 1978 tested cows, 56.02% of cases had positive reactions to the California mastitis test. The most common positive reaction we found in the milk was in one or two quarters with the intensity of the reaction of one and two plus. The most common causes of mastitis were staphylococci, streptococci and mixed infections. Continuing use of these methods it is possible to timely detect the presence of subclinical mastitis and get satisfactory results in the prevention and treatment of mastitis as well as increasing the quantity and improving the quality of milk.

scholarly journals Evaluation of sodium lauryl sulfate for the development of cow-side mastitis screening test

2021 ◽  
pp. 2290-2295
Author(s):  
Nobonita Sarker Tanni ◽  
Md. Shafiul Islam ◽  
Mojahidul Kabir ◽  
Mst. Sonia Parvin ◽  
Md. Amimul Ehsan ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Sodium Lauryl Sulfate ◽  
Early Stage ◽  
High Sensitivity ◽  
Subclinical Mastitis ◽  
Screening Tests ◽  
Bromocresol Purple ◽  
Lauryl Sulfate ◽  
Operating Characteristics ◽  
Milk Samples

Background and Aim: Subclinical mastitis (SCM) is an economically important disease for dairy cattle worldwide; therefore, regular screening is imperative to detect SCM at an early stage so as to control it. The study was conducted to compare the test characteristics of sodium lauryl sulfate (SLS) as a test reagent to detect SCM in dairy cows. Materials and Methods: First, 106 milk samples of dairy cows were subjected to available indirect screening tests (white side test [WST], surf field mastitis test, Leucocytest, and Immucell) considering somatic cell count (SCC) as gold standard test. Then 273 milk samples were allowed to react with different concentrations of SLS with or without sodium hydroxide (NaOH) and indicators (bromothymol blue [BTB] and bromocresol purple [BCP]). Results: SLS with or without NaOH yielded best reaction with the milk samples similar to Leucocytest. It was observed that the reaction of milk samples with SLS added with indicators (BTB and BCP) was easier to visualize than without indicators. SLS 3%+NaOH 1.5% with BTB and SLS 2% with BCP had high sensitivity, specificity, and substantial agreement with SCC. The area under the receiver operating characteristics curve of SLS 2% with BCP and SLS 3%+NaOH 1.5% with BTB was 0.917 and 0.875, respectively. Conclusion: It may be concluded that SLS 3%+NaOH 1.5% with BTB and SLS 2% with BCP may be the potential reagents for the development of an effective cow-side test to detect SCM, as the main ingredient SLS is considerably cheap and readily available in developing countries.

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