scholarly journals Merino ewe lambing after stimulation with prostaglandin and PMSG and insemination with sperm of grey Karakul rambs

2019 ◽  
Vol 6 (3) ◽  
pp. 28-36
Author(s):  
I. Lobachova ◽  
V. Yakovchuk
Keyword(s):  
Sexual Activity ◽  
Hormonal Stimulation ◽  
Time Treatment ◽  
White Spots ◽  
Total Fecundity ◽  
Prostaglandin F ◽  
Serum Gonadotropin ◽  
One Year ◽  
Ultrasound Testing ◽  
Lambing Rate

Aim. To determine the effectiveness of a two-time treatment with prostaglandin F-2α (PGF2α) followed by the injection of pregnant mare serum gonadotropin (PMSG) for stimulation of sexual activity in sheep in the second half of the estrous season in the scheme of increasing the lambing rate. Methods. Thirty-seven 2.5-yearold ewes of the Askanian Merino breed, which were kept together from the age of one year, were used. In September 2018, 28 animals of this group lambed and had sucklings until November 20. Hormonal stimulation was started on December 7, 2018 by injecting all ewes with 1 ml of synthetic PGF2α and 1 ml of Prozerin. The second injection of PGF2α was given 10 days after the fi rst one. Simultaneously with the second treatment with PGF2α, the animals were injected with 500 IE of PMSG (Sergon, Czech Republic) and 2.5 ml of SuperAminoC (South Korea). The presence of estrus was not detected, and the ewes were forcibly inseminated with freshly obtained sperm of 4 grey rams of the Askanian Karakul breed in the morning for three days, beginning with the fi rst day after the PMSG injection. During the fi rst treatment with PGF2α and 3 days before the second one, the genitals of 12 ewes were sonographically examined. Results. In total, 45.9 % of stimulated animals lambed; the total fecundity was 75.7 %, and the average prolifi cacy – 1.65 ± 0.18 lambs per ewe. Among the animals, which before the experiment had the sucklings, 57.1 % lambed, among those, who did not have the suckling, – 11.1 %. After using the sperm of ram 17314, 77.8 % of ewes lambed, for rams 73697, 73792 and 73796 – 30.0, 44.4 and 33.3 %, respectively. Among the 19 lambs analyzed by wool color, 10 lambs were completely black, 9 were black with white spots. Among the spotted lambs, the color on the back, forearms and hips of one lamb had an additional shade, close to gray. Ultrasound testing showed that the ewes, which before the experiment had sucklings, showed better genital reactivity. According to the distribution of lambs by wool color, an assumption about possible partial incompatibility of the genotypes of Askanian Merino ewes and grey Karakul sheep was made that requires further study. Conclusions. The scheme of stimulating sexual activity based on two-time treatment with PGF2α followed by an injection of PMSG is capable of ensuring lambing of more than 70 % of stimulated sheep with the prolifi cacy of 1.65 lambs per ewe. A factor, increasing the effectiveness of the scheme, is the presence of sucking lambs of ewes before the stimulation starts.

scholarly journals Effectiveness of a Selective Etching Technique in Reducing White Spots Formation around Lingual Brackets: A Prospective Cohort Clinical Study

Coatings ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 572
Author(s):  
Domenico Dalessandri ◽  
Marino Bindi ◽  
Francesca Massetti ◽  
Gaetano Isola ◽  
Marco Migliorati ◽  
...  
Keyword(s):  
Clinical Study ◽  
Difficulty Level ◽  
Prospective Clinical Study ◽  
White Spot Lesions ◽  
Etching Technique ◽  
White Spots ◽  
Accumulation Index ◽  
One Year ◽  
Lingual Brackets ◽  
And Control

The risk of developing white spot lesions (WSLs) after orthodontic treatment with lingual brackets is generally considered lower than with labial ones, even if plaque accumulation is frequently higher due to the increased difficulty level in oral hygiene maintenance. In this prospective clinical study, selective enamel etching technique effectiveness in reducing plaque accumulation and WSLs was tested. Thirty patients were bonded with a split-mouth approach: two randomly selected opposite quadrants were used as the test sides, using customized plastic etching guides, and the other two as control sides, applying traditional direct etching methods. The plaque presence around the braces was recorded after 1, 3, 6, and 12 months according to a lingual plaque accumulation index (LPAI), as was the presence of WSLs. PAI measured values were significantly higher in the control sides during the observation period. Test and control sides differed significantly for new WSL onset only after 12 months of treatment. Therefore, the present research demonstrated that this guided enamel etching technique allowed for significant reduction in plaque accumulation around the lingual brackets and reduced onset of white spots after one year of treatment.

Positive hCG in a patient who reports no sexual activity for one year

2014 ◽  
pp. 132-133
Author(s):  
Roger P. Smith ◽  
David Chelmow ◽  
Christine R. Isaacs ◽  
Ashley Carroll
Keyword(s):  
Sexual Activity ◽  
One Year

scholarly journals Brief Note: Effects of Prostaglandins, Epinephrine and NaF on Human Leukocyte, Platelet and Liver Adenyl Cyclase

Blood ◽  
1970 ◽  
Vol 35 (4) ◽  
pp. 514-516 ◽  
Author(s):  
ROBERT E. SCOTT
Keyword(s):  
Cyclic Amp ◽  
Phagocytic Activity ◽  
Prostaglandin E1 ◽  
Human Leukocyte ◽  
Adenyl Cyclase ◽  
Hormonal Stimulation ◽  
Human Leukocytes ◽  
Prostaglandin F ◽  
Limited Scope

Abstract Despite the limited scope of this study, the data show that homogenates of human leukocytes, platelets and liver are capable of synthesizing cyclic AMP, and that such preparations are responsive to hormonal stimulation. Epinephrine stimulates cyclic AMP production in liver and leukocyte homogenates, and NaF shows a stimulatory effect in each tissue.1,8 The data confirm the previous reports10,11 that prostaglandin E1 stimulates platelet adenyl cyclase, but further shows a lack of stimulation by prostaglandin F/1β. Incubation of leukocytes with the prostaglandins showed a similar effect. It is suggested that further study may establish a relationship between adenyl cyclase stimulation and chemotaxis or phagocytic activity of circulating leukocytes as has been reported in amebae.12 Since systemic enzyme deficiencies such as phosphorylase in Hers’ Disease13 and amylo-1.4→1.6-transglucosidase in Andersens’ Disease14 are evident in leukocytes, it is suggested that further study of circulating leukocytes may also detect an abnormal responsiveness or a deficiency of adenyl cyclase.

The role of gonadal steroids in feedback regulation of gonadotropin secretion at different stages of primate development

1987 ◽  
Vol 114 (2) ◽  
pp. 257-268 ◽  
Author(s):  
J. S. D. Winter ◽  
L. Ellsworth ◽  
G. Fuller ◽  
W. C. Hobson ◽  
F. I. Reyes ◽  
...  
Keyword(s):  
Feedback Inhibition ◽  
Feedback Regulation ◽  
Serum Testosterone ◽  
Significant Rise ◽  
Sexually Dimorphic ◽  
Adult Males ◽  
Gonadotropin Secretion ◽  
Central Inhibition ◽  
Serum Gonadotropin ◽  
One Year

Abstract. The serum gonadotropin response to castration was assessed in 8 foetal, 2 neonatal, 30 juvenile, and 2 adult rhesus monkeys (M. mulatta). In the 30 castrated juvenile monkeys and 8 sham-operated controls, concentrations of oestrone, oestradiol, androstenedione, dihydrotestosterone, testosterone and 17OH-progesterone were measured in 10 ml serum pools before, one month after, and one year after the surgical procedure. Castration during foetal life (83–137 days gestation) was followed within 48–72 h by a significant rise in serum FSH levels in males, but had no effect on the already high levels in females. Similarly, castration of males during the first post-natal month raised serum FSH and LH into the adult castrate range; however, after 3 months of age serum gonadotropin levels again declined to the normal juvenile range in spite of the open feedback loop. Orchiectomy of pre-pubertal juvenile monkeys (age 3 months–28/12 years) had no immediate effect on serum gonadotropins, but was followed by a delayed rise in FSH (at age 23/12–43/12 years) and LH (at age 27/12–44/12 years) to adult castrate levels. Orchiectomy of older prepubertal (by serum testosterone) or adult males resulted within a few days in a progressive and sustained rise in serum FSH and a more gradual rise in LH. Prepubertal gonadotropin regulation appeared to be sexually dimorphic, since ovariectomy in juvenile females (age 3 months–15/12 years) was followed by generally elevated, if somewhat erratic, serum FSH values, with a secondary rise in both FSH and LH levels at 2–21/12 years. In both sexes, prepubertal castration caused a significant and sustained decline in serum concentrations of oestradiol; castrated males also showed a decline in serum testosterone levels. Although prepubertal castration also caused in both sexes a slight decline in serum oestrone, and ovariectomy a decline in serum androstenedione and dihydrotestosterone, these effects were not sustained one year later, and values were not significantly different from sham-operated controls. Taken together, these data lend support to a model of primate sexual maturation in which the primary regulator of gonadotropin secretion in both sexes during the prolonged juvenile phase is central inhibition of the hypothalamic GnRH regulator. However, during foetal and neonatal life, and again following the onset of puberty, the major modulator of gonadotropin secretion becomes sex steroid-mediated feedback inhibition.

THE SERUM LEVELS OF PROGESTERONE AND 20α-DIHYDROPROGESTERONE, AND THE OVARIAN LH, FSH AND PRL BINDING DURING LUTEOLYSIS OF THE SUPERLUTEINIZED RAT OVARY

1977 ◽  
Vol 86 (1) ◽  
pp. 162-172 ◽  
Author(s):  
Peter A. Torjesen ◽  
Asbjørn Aakvaag
Keyword(s):  
Binding Sites ◽  
Ovarian Tissue ◽  
Serum Levels ◽  
Plasma Progesterone ◽  
Luteal Function ◽  
Prostaglandin Analogues ◽  
Prostaglandin F ◽  
Serum Gonadotropin ◽  
Rat Ovary

ABSTRACT The process of luteolysis has been studied in immature rats in which superluteinization had been induced with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotrophin (HCG). Following prostaglandin F2α and the prostaglandin analogues, and at the end of the pseudopregnancy, the plasma levels of progesterone and 20α-dihydroprogesterone were measured and used as parameters of luteal function in relation to the capacity of the ovarian tissue to bind LH, FSH and prolactin (PRL) in vitro. On day 19 after HCG a marked decrease in the progesterone level from the day 8 level was observed concomitant with a marked increase in 20α-dihydroprogesterone. The capacity of the ovarian tissue to bind LH in vitro was markedly reduced on day 19 compared to day 8. Identical changes were observed 21 h after 1 mg prostaglandin F2α or prostaglandin analogues. Progesterone decreased from about 600 ng/ml to about 50 ng/ml, whereas the increase in 20α-dihydroprogesterone was from about 200 ng/ml to 500–1000 ng/ml and the reduction in LH binding sites was from 1.7 × 10−12 to 0.5 × 10−12 mol/mg protein. Nanogram amounts of the analogues were as effective as 1 mg of prostaglandin F2α. The number of FSH or PRL binding sites was not affected by spontaneous luteolysis or the treatment given. By the use of graded doses of the prostaglandin analogues a negative correlation (r=−0.81) was found between plasma progesterone and 20α-dihydroprogesterone levels, and a positive correlation (r = 0.84) between LH binding sites and plasma progesterone levels. The luteolysis induced by prostaglandin F2α or prostaglandin analogues was indistinguishable from the spontaneous luteolysis using these parameters.

A polymorphism at the melatonin receptor 1A (MTNR1A) gene in Sarda ewes affects fertility after AI in the spring

2011 ◽  
Vol 23 (2) ◽  
pp. 376 ◽  
Author(s):  
Vincenzo Carcangiu ◽  
Sebastiano Luridiana ◽  
Giuseppe Massimo Vacca ◽  
Cinzia Daga ◽  
Maria Consuelo Mura
Keyword(s):  
Gene Polymorphisms ◽  
Genomic Dna ◽  
Allelic Frequency ◽  
Melatonin Receptor ◽  
Transabdominal Ultrasonography ◽  
Genotype Group ◽  
Reproductive Response ◽  
Serum Gonadotropin ◽  
Gene Exon ◽  
Lambing Rate

The effect of MTNR1A gene polymorphisms on the fertility rate after AI in Sarda sheep was evaluated in 600 lactating adult ewes. Genomic DNA was subjected to amplification of the MTNR1A gene exon II. Amplicons were digested with restriction endonuclease MnlI. Ten samples from each genotype were sequenced. A polymorphism was detected (A612G) and ewes were determined to be +/+, +/– or –/– for the allele. Allelic frequency was 0.77 for the + allele and 0.23 for the – allele. The frequency of the +/+, +/– and –/– genotypes was 68, 19 and 13%, respectively. On 16 May 2009, 60 ewes from each genotype group were synchronised using intravaginal sponges containing 40 mg fluorogestone acetate for 14 days. At sponge removal, the ewes were administered 350 IU pregnant mare’s serum gonadotropin and were then inseminated, 54–56 h later, with 400 × 106 spermatozoa. Pregnancies were confirmed 50 days after AI using transabdominal ultrasonography. Lambing dates and the number of newborn lambs were recorded within 155 days after AI. Conception and lambing rate were higher for ewes with the +/+ and +/– genotypes compared with those with the –/– genotype (P < 0.01). In conclusion, there was a positive correlation between MTNR1A allele polymorphisms the reproductive response following synchronisation and AI in the spring.

Psychological Effect of Semi-permanent Tattooing Rehabilitation in Patients with Mastectomy in 12 Months Period

2017 ◽  
Vol 41 (S1) ◽  
pp. S669-S669
Author(s):  
P. Argitis ◽  
P. Platari ◽  
K. Gatsiou ◽  
C. Chatzidai ◽  
K. Paschalidis
Keyword(s):  
Quality Of Life ◽  
Body Image ◽  
Sexual Activity ◽  
Rating Scale ◽  
Cancer Type ◽  
Body Image Scale ◽  
Scale Scores ◽  
Competing Interest ◽  
One Year

IntroductionBreast cancer is the most common cancer type in Greek women as more than 4000 new cases are diagnosed every year. Seventy percent of those patents performs a type mastectomy. The breast has a societal and social connotation of femininity, motherhood, and sexuality.BackgroundSeveral studies support the existence of the relationship between psychological problems and mastectomy surgery. Body image and feminine self-concept also seems to influence quality of life of those women, considering the breast association of femininity, motherhood, and sexuality. During this study, we try to investigate how a non-psychiatric intervention might influence the mental state and the quality of life of those women.MaterialA clinical interview was performed in 53 women with partial or total mastectomy before 3 and 52 weeks after the rehabilitation with the method of semi-permanent tattooing.MethodsData were collected during the personal interviews, using Hamilton anxiety rating scale (Ham-A), body image scale and sexual activity questionnaire.ResultsModerate levels of anxiety were identified before the rehabilitation, associated with poor body image scale scores and sexual difficulties. Both Ham-A and body image score ameliorated after 3 weeks with unchanged sexual behaviour. One year after rehabilitation, anxiety scale score raises close to initial values, body image remains unchanged, comparing with the 3rd week interview and significant improvement noticed in sexual activity.Disclosure of interestThe authors have not supplied their declaration of competing interest.

Advancements in Treatment Options of Women Infertility

2020 ◽  
Vol 16 ◽  
Author(s):  
Nahid Lorzadeh ◽  
Yasaman Kazemirad ◽  
Nastran Kazemirad
Keyword(s):  
Polycystic Ovarian Syndrome ◽  
Treatment Options ◽  
Unprotected Sex ◽  
Fallopian Tubes ◽  
Hormonal Stimulation ◽  
Fertile Ground ◽  
Age Factor ◽  
One Year ◽  
Assistive Reproductive Technology ◽  
Ovarian Syndrome

: Infertility is defined as inability to conceive pregnancy after one year or more of unprotected sex, among the women at the risk of conceiving pregnancy. In addition, to age-factor, it is known to be associated with pathological and genetic conditions that contribute to its early onset. Polycystic ovarian syndrome (PCOS), endometriosis, fibroids and blockade in fallopian tubes are certain common pathological causes of female infertility. Researches are focused to eradicate primary cause of infertility in order to achieve healthy fertile ground for fertilization. Depending on the cause, therapeutic options include, medicines (chemical, herbal and Chinese), hormonal stimulation, surgery, assistive reproductive technology and tissue engineering. To the extent of research, biotechnology has played significant role to answer the problem. This review discusses advancements in these therapeutic fields, in order to treat women-associated infertility.

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