Development of a High-Throughput DNA Fiber Analysis Device Using MEMS Technology

Cardiac tissue engineering is currently limited by the incapacity to test the wide range of parameters that might impact the engineered tissue in a high throughput and combinatorial manner. Here we used microelectromechanical systems (MEMS) technology to generate arrays of cardiac microtissues (CMTs) embedded within three-dimensional micropatterned matrices. Microcantilevers constrain CMT contraction and report generated forces. We demonstrate the ability to routinely produce ∼200 CMTs per million cardiac cells whose spontaneous contraction frequency, duration, and forces can be tracked. Independently varying the mechanical stiffness of the cantilevers and collagen matrix revealed that the CMT contractility increased with boundary or matrix rigidity. We also show that the combination of electrical stimulation and auxotonic load strongly improve both the structure and the function of the CMTs. Finally, we demonstrate the suitability of our technique for high throughput monitoring of drug-induced changes in spontaneous frequency or contractility in CMTs.

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E-1-3 Chromosome Stretching Characterization of High-Throughput DNA Fiber Analysis Device using centrifugal force

The Proceedings of the Conference on Information Intelligence and Precision Equipment IIP ◽

10.1299/jsmeiip.2014._e-1-3-1_ ◽

2014 ◽

Vol 2014 (0) ◽

pp. _E-1-3-1_-_E-1-3-3_

Author(s):

Kodai KITANO ◽

Yuki NITTA ◽

Kyohei TERAO ◽

Hidekuni TAKAO ◽

Fusao SHIMOKAWA ◽

...

Keyword(s):

Centrifugal Force ◽

High Throughput ◽

Fiber Analysis

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Study on Gold Nanoparticles Aggravating MEMS Cantilever Gene Detection Biosensor

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.336-338.148 ◽

2013 ◽

Vol 336-338 ◽

pp. 148-152

Author(s):

Yue Tao Ge ◽

Xiao Tong Yin ◽

Chao Bang Guo ◽

Bo Yang

Keyword(s):

Gold Nanoparticles ◽

Nucleic Acid ◽

High Throughput ◽

Layer By Layer ◽

Gene Detection ◽

Detection Level ◽

Target Dna ◽

Mems Technology ◽

High Throughput Detection ◽

Micro Cantilever

In order to detect whether there are nucleic acid components interested in the testing samples, this study manuscript presents a method and a process based on MEMS technology and biosensor technology. Although MEMS micro cantilever is very sensitive for stress mass, we must aggravate the cantilever for the reason of microscopic DNA mass is very weak. In order to achieve the purpose of their molecules detection level, gold nanoparticles (GNPs) which are combined with hybridization information is taken to aggravate MEMS cantilever, and we employ Au - S chemical bond of GNPs and dithiol HS(CH2)6SH to combine and fix DNA probe, and then employ target DNA which is marked with biotin to combine GNPs by Biotin - Streptavidin combining. We use the method of layer by layer to amplify the signal again until meet the hypersensitized requirements. Last we apply multi-channel microfluidic technology to achieve high-throughput detection.

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