Evaluation of a Sensor Detection Ability and Reactivity of Antigen-antibody Reaction on Surface of a Polymer Sheet

2019 ◽  
Vol 139 (9) ◽  
pp. 303-309
Author(s):  
Takaaki Isoda ◽  
Hiroki Ichihara ◽  
Kodai Ryujin
Keyword(s):  
Detection Ability ◽  
Polymer Sheet ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Sensor Detection

scholarly journals Evaluation of Shear Horizontal Surface Acoustic Wave Biosensors Using “Layer Parameter” Obtained from Sensor Responses during Immunoreaction

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4924
Author(s):  
Koji Kano ◽  
Hiromi Yatsuda ◽  
Jun Kondoh
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Horizontal Surface ◽  
Propagation Characteristics ◽  
Viscosity Change ◽  
Layer Parameter ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Shear Horizontal ◽  
Molecular Dimensions

Shear horizontal surface acoustic wave (SH-SAW) biosensors measure the reaction of capture antibodies immobilized on the sensing surface to capture test molecules (antigens) by using the change in SH-SAW propagation characteristics. SH-SAW displacement exists not only on the SH-SAW propagating surface, but also partially penetrates the specimen liquid to a certain depth, which is determined by the liquid properties of the specimen and the operating frequency of the SH-SAW. This phenomenon is called viscosity penetration. In previous studies, the effect of viscosity penetration was not considered in the measurement of SH-SAW biosensors, and the mass or viscosity change caused by the specific binding of capture antibodies to the target antigen was mainly used for the measurement. However, by considering the effect of viscosity penetration, it was found that the antigen–antibody reaction could be measured and the detection characteristics of the biosensor could be improved. Therefore, this study aims to evaluate the detection properties of SH-SAW biosensors in the surface height direction by investigating the relationship between molecular dimensions and SH-SAW propagation characteristics, which are pseudo-changed by varying the diameter of gold nanoparticles. For the evaluation, we introduced a layer parameter defined by the ratio of the SH-SAW amplitude change to the SH-SAW velocity change caused by the antigen–antibody reaction. We found a correlation between the layer parameter and pseudo-varied molecular dimensions. The results suggest that SH-SAW does not only measure the mass and viscosity but can also measure the size of the molecule to be detected. This shows that SH-SAW biosensors can be used for advanced functionality.

scholarly journals 1P089 Antigen-Antibody-Reaction Analysis by Fluorescence Correlation Spectroscopy

2004 ◽  
Vol 44 (supplement) ◽  
pp. S52
Author(s):  
H. Sakata ◽  
H. Morioka ◽  
M. kinjo ◽  
M. Tamura
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Antibody Reaction ◽  
Antigen Antibody

scholarly journals Investigation of the Mechanism for Rapid Antigen-Antibody Reaction Using Electric Field Mixing (EFM)

2019 ◽  
Vol 85 (2) ◽  
pp. 208-212
Author(s):  
Ryuta NAKAMURA ◽  
Takayuki KUSUMI ◽  
Yoshinobu OKUBO ◽  
Hiroshi NANJO ◽  
Yoshihiro MINAMIYA ◽  
...  
Keyword(s):  
Electric Field ◽  
Antibody Reaction ◽  
Antigen Antibody

The study of the antigen-antibody reaction by fluorescence method in LB films for immunosensor

1997 ◽  
Vol 307 (1-2) ◽  
pp. 294-297 ◽  
Author(s):  
G.K. Chudinova ◽  
A.V. Chudinov ◽  
V.V. Savransky ◽  
A.M. Prokhorov
Keyword(s):  
Fluorescence Method ◽  
Lb Films ◽  
Antibody Reaction ◽  
Antigen Antibody

Antigen-antibody reaction

1991 ◽  
pp. 32-35
Author(s):  
A. Paraf ◽  
G. Peltre
Keyword(s):  
Antibody Reaction ◽  
Antigen Antibody

SERUM COMPLEMENT IN ACUTE GLOMERULONEPHRITIS AND THE NEPHROTIC SYNDROME

PEDIATRICS ◽  
1951 ◽  
Vol 8 (6) ◽  
pp. 814-820
Author(s):  
KURT LANGE ◽  
LAWRENCE SLOBODY ◽  
FRANK GRAIG ◽  
GLORIA OGUR ◽  
JACOB OBERMAN ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Normal Values ◽  
Acute Glomerulonephritis ◽  
Serum Complement ◽  
A Value ◽  
Acth Therapy ◽  
Antibody Reaction ◽  
Antigen Antibody

The literature on glomerulonephritis as an antigen-antibody reaction has been briefly reviewed. This reaction is complement-binding. A method to determine complement titres has been described and a unit of complement defined. The value for complement in 52 control children ranged between 0.84 and 3.0 units with an average of 1.68 units; only one child showed a value below 1.0 units. The complement titre in five cases of acute glomerulonephritis in children ranged between 0.08 and 1.0 units with an average of 0.43 units. Similar values were found in eight adult cases. The complement titre in nine cases of nephrosis in children ranged between 0.09 and 1.0 unit with an average of 0.57 units. No immunologic differences were observed between the nephrotic syndrome in glomerulonephritis and so-called pure nephrosis as far as complement was concerned. Complement levels return to normal approximately one week after the subsidence of clinical and laboratory signs of activity of glomerulonephritis. Diuresis, whether spontaneous or induced by ACTH therapy, is accompanied by a return of complement to normal values in spite of persistence of the same degree of proteinuria, hypoproteinemia and hypercholesterolemia.

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