The Latex Fixation Test. II. Results in Rheumatoid Arthritis

PEDIATRICS ◽  
1957 ◽  
Vol 19 (4) ◽  
pp. 579-579
Keyword(s):  
Rheumatoid Arthritis ◽  
Lupus Erythematosus ◽  
Gamma Globulin ◽  
Agglutination Test ◽  
Polystyrene Latex ◽  
Agglutination Reaction ◽  
Latex Particles ◽  
Naked Eye ◽  
Rheumatic Heart ◽  
Latex Test

This test, which has been proposed for use in diagnosis of rheumatoid arthritis, depends upon the use of polyvinyl toluene or polystyrene latex particles serving as carriers of fraction II of serum (gamma globulin) in an agglutination reaction with patient's serum. The addition of latex particles mixed with gamma globulin is added to progressive dilutions of the serum to be tested. The resultant visible agglutination is read with the naked eye. Seventy-one per cent of the serums from 150 patients with rheumatoid arthritis gave positive agglutination. Of 250 patients with rheumatic fever and rheumatic heart disease, only 1.6% gave a positive agglutination test. The agglutination test is positive in a small percentage of other diseases, such as hyperglobulinemia and lupus erythematosus. The results of the latex test compare favorably with the hemagglutination test which employs sheep erythrocytes. The latter test is more difficult to perform and to control. Both tests are positive in a smaller percentage of individuals with juvenile rheumatoid arthritis, which has not been explained.

scholarly journals Detection of Rabies Virus Antigen in Dog Saliva Using a Latex Agglutination Test

2000 ◽  
Vol 38 (8) ◽  
pp. 3098-3099 ◽  
Author(s):  
S. Kasempimolporn ◽  
W. Saengseesom ◽  
B. Lumlertdacha ◽  
V. Sitprija
Keyword(s):  
Rabies Virus ◽  
Gamma Globulin ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Virus Antigen ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Glass Slide ◽  
Human Rabies ◽  
Latex Particles

Dog bites are responsible for more than 90% of human rabies deaths in Asia. We developed a simple and inexpensive test based on latex agglutination (LA) for rabies virus antigen detection in dog saliva. Rabies virus antigen could be detected by agglutination on a glass slide using latex particles coated with gamma globulin. By evaluation of paired saliva-brain specimens from 238 dogs, the LA test using saliva was 99% specific and 95% sensitive compared to the fluorescent antibody test (FAT) on brain smears. The advantages of the LA test over the standard FAT are that it is comparatively simple and there is no need to kill the animal before examination.

Evaluation of a Rapid Latex-Agglutination Test for Detection of α1-Fetoprotein

1973 ◽  
Vol 19 (9) ◽  
pp. 1069-1070 ◽  
Author(s):  
E Alpert ◽  
R L Coston
Keyword(s):  
Liver Diseases ◽  
Gamma Globulin ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Agglutination Assay ◽  
Large Numbers ◽  
Positive Results ◽  
Normal Controls ◽  
Latex Agglutination Assay ◽  
Latex Test

Abstract A latex-agglutination assay for α-fetoprotein (AFP) has been evaluated and compared to the results obtained by counter-immunoelectrophoresis (CIEP). Both of these tests must be performed on both undiluted and diluted sera, to avoid prozone effects. Agglutination of control latex, coated with normal rabbit gamma globulin, by sera from 8% of hepatoma patients and 18% of patients with other liver diseases makes the test inconclusive in these patients. Nevertheless, there was 88.6% agreement with CIEP in the detection of AFP in a series of 50 hepatoma patients. There were no false positives for sera from 98 patients with other liver diseases or for normal controls. We conclude that latex agglutination is a simple, specific way to screen large numbers of sera for AFP, if all positive results are confirmed by a control latex test and CIEP.

Evaluation of a Latex Agglutination Test for Rapid Identification of Staphylococcus aureus from Foods

1993 ◽  
Vol 56 (9) ◽  
pp. 759-762 ◽  
Author(s):  
TSUNG C. CHANG ◽  
SU H. HUANG
Keyword(s):  
Staphylococcus Aureus ◽  
Immunoglobulin G ◽  
Protein A ◽  
Rapid Identification ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Polystyrene Latex ◽  
Latex Agglutination Test ◽  
Overnight Incubation ◽  
Latex Test

A latex agglutination kit (AUREUS TEST™) for the rapid identification of Staphylococcus aureus was evaluated in this study. The reagent consists of polystyrene latex particles sensitized with rabbit anti-protein A immunoglobulin G and fibrinogen. Due to the respective binding of bacterial protein A with immunoglobulin G and coagulase with fibrinogen, an agglutination reaction occurs within 1 min when the latex is mixed with a suspension of S. aureus. Of 157 S. aureus isolates (138 from foods) and 110 non-S. aureus isolates (58 species belonging to 19 genera), the sensitivity and specificity of the latex test were 100 and 94.4–100%, respectively. The results were comparable to the conventional coagulase test. Therefore, it is proposed that suspicious colonies of S. aureus on Baird-Parker agar medium be subcultured to tryptic soy agar for overnight incubation. Cultures grown on tryptic soy agar are used for latex agglutination test. The latex agglutination test can be finished within minutes and could be used as an alternative rapid procedure of the coagulase test, which needs several hours or even overnight incubation for completion. In addition, all S. aureus isolates grown on Baird-Parker agar also could be correctly identified by the latex reagent.

Increased Protein Synthesis by Human Platelets during Phagocytosis of Latex Particles in Vitro

1976 ◽  
Vol 35 (02) ◽  
pp. 350-357 ◽  
Author(s):  
Hana Bessler ◽  
Galila Agam ◽  
Meir Djaldetti
Keyword(s):  
Protein Synthesis ◽  
Fold Increase ◽  
Human Platelets ◽  
Polystyrene Latex ◽  
Latex Particles ◽  
The Third ◽  
Platelet Protein ◽  
Dose Dependent ◽  
Phagocytotic Activity

SummaryA three-fold increase of protein synthesis by human platelets during in vitro phagocytosis of polystyrene latex particles was detected. During the first two hours of incubation, the percentage of phagocytizing platelets and the number of latex particles per platelet increased; by the end of the third hour, the first parameter remained stable, while the number of latex particles per cell had decreased.Vincristine (20 μg/ml of cell suspension) inhibited platelet protein synthesis. This effect was both time- and dose-dependent. The drug also caused a decrease in the number of phagocytizing cells, as well as in their phagocytotic activity.

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