scholarly journals Тканиноспецифічність впливу фізичних та фармакологічних чинників на антиоксидантний захист у тварин з ерозивно-виразковою патологією гастродуоденальної зони

2018 ◽  
Vol 8 (1) ◽  
pp. 371-378
Author(s):  
L. Ponomarenko ◽  
O. Lykholat ◽  
O. Khomenko
Keyword(s):  
Magnetic Field ◽  
Free Radical ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Single Point ◽  
Experimental Investigations ◽  
Rat Tissues ◽  
Antioxidant Defense Systems ◽  
Gastroduodenal Zone

<p><span lang="EN">Influence of the natural magnetic field (MP) is an integral part of the existence of all living systems on Earth. Artificial MPS appeared precisely due to human activity. It is established that the interaction of MP with biosystems, its biological activity depends on the biotropic parameters of this field, such as frequency, intensity, gradient (rate of growth), vector, pulse shape, exposure time and localization of influence. </span><span lang="EN">There is now no single point of view regarding the mechanisms of interaction of the MP with biological objects. Experimental investigations have documented the influence of MP on the reactions occurring in the free radical type. Free radical pathologies include diseases such as gastroesophageal reflux disease and peptic ulcer disease. Therefore, the search for factors that can affect the components of the system of oxidative homeostasis remains. </span><span lang="EN">The purpose of the work is to evaluate the isolated effect of the magnetic field and in combination with injections of L-arginine-L-glutamate into lipoperoxidation processes and antioxidant defense system in rat tissues with experimental pathology of the gastroduodenal zone. The modeling of erosive-ulcerative lesions of the gastroduodenal zone in rats was accompanied by an imbalance in the functioning of antioxidant defense systems and the intensification of lipoeroxidation LPO processes in the blood, liver, brain and stomach tissues of experimental animals. Applications of a magnetic field to animals with simulated pathology of the gastroduodenal zone resulted in inhibition of the processes of LPO in the blood of experimental rats at the activation of free radical reactions in the liver, brain and stomach tissues, on the background of partial decontamination of the glutathione system enzymes against the backdrop of an increase in the amount of reduced glutathione. With the combined action of glutargin and magnetic field in the blood, liver and brain tissues of experimental rats with erosive-ulcerative defeat of the gastroduodenal zone, activation of anti-radical, anti-peroxide protection enzymes, increased levels of reduced glutathione against the inhibition of glutathione reductase activity and a decrease in the intensity of the LPO processes with simultaneous normalization </span><span lang="EN-US">of </span><span lang="EN">processes of lipoperoxidation in the stomach tissue took place.</span></p>

scholarly journals Cytoprotective processes induced by the effect of L-arginin-L-glutamate in rats with experimental pathology of the gastroduodenal zone

2018 ◽  
Vol 9 (2) ◽  
pp. 300-307
Author(s):  
Y. M. Stepanov ◽  
L. A. Ponomarenko ◽  
O. A. Lykholat ◽  
T. M. Shevchenko ◽  
O. M. Khomenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Experimental Animals ◽  
Free Radical Processes ◽  
Experimental Pathology ◽  
Ulcerative Lesions ◽  
Gastroduodenal Zone ◽  
Radical Processes

The processes of effect of L-arginine-L-glutamate on peroxidation and slime-forming function of the stomach cells, the system of antioxidant defense in the blood, liver and brain tissues of rats with experimental pathology of the gastroduodenal zone have been investigated. The animals were divided into four groups. Group I – control group were rats injected intragastrically through a probe physiological solution. Group II included animals with erosive ulcerative lesions of the gastroduodenal zone. Modeling of the erosive ulcerative lesions was carried out by intragastric administration of medical bile (1 ml/100 g) in combination with immobilization-cold stress for 1 hour at + 4 ºС for a period of seven days. Rats of group ІІІ simultaneously received an intra-abdominal 4% solution of L-arginine-L-glutamate in a dose of 20 mg per 100 g of body weight at the same time as the erosive ulcerative lesions modeling. To clarify the role of NO-ergic mechanism of L-arginine-L-glutamate influence on the quantitative composition of mucins and free radical processes rats in group ІV with erosive ulcerative lesions were injected with non-selective NO-synthase inhibitor, L-NAME (L-NG-nitroarginine methyl ester), at a dose of 1 mg per 100 g at the same time as injections of 4% solution of L-arginine-L-glutamate. The simulation of erosive-ulcerative lesions of the gastroduodenal zone in the experimental animals was accompanied by the intensification of lipid peroxidation processes, the imbalance of the antioxidant defense systems and the development of oxidative stress in the blood, tissues of the stomach, liver and brain, which has tissue-specific features. In the blood of the animals, the activation of the enzymatic link of antioxidant defense did not compensate for free radical processes, as a result, the exhaustion of the reduced glutathione pool occurred, and the level of TBA-active products increased both in plasma and in erythrocytes. There was a depression of the enzymes of the antioxidant defense and a decrease in the level of recovered glutathione, indicating decompensating of the liver antioxidant protection systems in the liver tissue of the rats. In the experimental animals , formation of erosive ulcerative lesions was accompanied by destabilization of the oxidation-reducing processes in the brain, which led to the intensification of the lipoperoxidation. In the mucous membrane of the stomach of the experimental animals, the total number of protection factors – secretory mucins with a simultaneous structural change – decreased. The use of L-arginine-L-glutamate reduced the manifestations of oxidative stress in the stomach tissue of animals with experimental pathology and normalized the quantitative and qualitative composition of mucins. In the blood, liver tissues and brain of the rats, L-arginine-L-glutamate injections activated the enzymes of the first anti-radical linkage – superoxide dismutase and catalase contributed to the increase of the pool of reduced glutathione and the deceleration of free radical reactions. Investigation of reactions to the action of the inhibitor provides the basis for the hypothesis of the NO-mediated action of L-arginine-L-glutamate on the formation of S-nitrosothiols, as evidenced by the high level of reduced glutathione when the inhibitor is used.

scholarly journals Effect of the Supplement of Humic Origin on the Free Radical Processes and Histological Changes in the Tissues of Rats Affected by Chromium (VI)

2020 ◽  
Vol 11 (3) ◽  
pp. 10996-11008
Keyword(s):  
Body Weight ◽  
Free Radical ◽  
Antioxidant Defense ◽  
Reductase Activity ◽  
Antioxidant Defense System ◽  
Rat Tissues ◽  
Defense System ◽  
Free Radical Processes ◽  
Chromium Vi ◽  
Radical Processes

The goal of our study was to examine the effect of feed supplement Humilid on the antioxidant defense system, free radical processes, and histomorphological changes in the tissues of rats affected by Chromium (VI). Rats were divided into 4 groups. Animals of groups D2 and D3 received Humilid in a dose of 2 ml per kg of body weight for 28 days. From the 14th day of the experiment, rats of groups D1 and D2 were injected by K2Cr2O7 in a dose of 2 mg Cr per kg of body weight daily for 14 days. Control animals were injected with saline solution for 14 days. Results showed that Cr (VI) causes oxidative stress in rat tissues, accompanied by an increase in superoxide dismutase activity, lipid peroxidation products, and a decrease in glutathione peroxidase and glutathione reductase activity. Chromium also caused histopathological changes in the liver and kidney of rats. Watering of rats affected by Cr (VI) with Humilid leads to decreased oxidative processes, activation of the antioxidant defense system, and reduced pathological effects on animal tissues. Our results indicate the positive influence of Humilid on the rat’s organism, inhibiting free radical processes and exhibiting antioxidant, hepatoprotective, and adaptive properties.

scholarly journals Antioxidant status in children and adolescents with COVID-19

2021 ◽  
Vol 6 (6-2) ◽  
pp. 29-36
Author(s):  
L. V. Rychkova ◽  
M. A. Darenskaya ◽  
N. V. Semenova ◽  
S. I. Kolesnikov ◽  
A. G. Petrova ◽  
...  
Keyword(s):  
Children And Adolescents ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Control Group ◽  
Negative Consequences ◽  
Spectrophotometric Methods ◽  
Total Antioxidant ◽  
Therapeutic Measures ◽  
Glutathione Reductase Activity

Background. The COVID-19 pandemic has raised the importance of this problem to the first stage and has affected healthcare system around the world. Despite the more favorable COVID-19 course, the child population should be at focus of special attention, due to the active participation in its distribution. The course of COVID-19 includes a cascade of pathological processes accompanied by the generation of reactive oxygen species, which can have extremely negative consequences for the developing organism. The research of these processes in children is vital and will improve the effectiveness of preventive and therapeutic measures. The aim: to analyze changes in enzymatic and non-enzymatic links in the antioxidant defense in children and adolescents with diagnosed COVID-19 infection.Materials and methods. 17 children and adolescents (average age – 12.35 ± 4.01 years) were examined, including 8 boys (47 %) and 9 girls (53 %) with COVID-19 infection. The control group of children and adolescents (practically healthy) according to the «copy-pair» principle was selected. We used spectrophotometric methods.Results. In the group of children and adolescents with diagnosed COVID-19 infection, there were lower levels of total antioxidant activity (p < 0.0001), superoxide dismutase activity (p < 0.0001), content of reduced glutathione (p = 0.048) and retinol (p = 0.015), increase in glutathione reductase activity (p = 0.015) relative to the control.Conclusion. The obtained data indicate the insufficiency of antioxidant system components number in children and adolescents with diagnosed COVID-19 infection and indicate the advisability of antioxidant therapy using to stabilize these indicators.

POSSIBILITIES OF ANTIOXIDANT PROTECTION IN PATIENTS WITH SUPERIOR HYPERPLASIA OF THE PEDESTAL GLAND AND ACCOMPANYING CHRONIC POSTATITIS

2020 ◽  
pp. 8-11
Author(s):  
Павел Георгиевич Осипов ◽  
Андрей Александрович Береш ◽  
Юрий Сергеевич Ханин ◽  
Олеся Игоревна Некрылова
Keyword(s):  
Lipid Peroxidation ◽  
Chronic Prostatitis ◽  
Antioxidant Defense ◽  
Prostate Gland ◽  
Antioxidant Defense System ◽  
Complex Treatment ◽  
Traditional Treatment ◽  
Important Place ◽  
Antioxidant Defense Systems ◽  
Benign Hyperplasia

Несмотря на достигнутые успехи в диагностике и лечении, на сегодняшний день проблема хронического простатита у пациентов с доброкачественной гиперплазией простаты продолжает оставаться актуальной. Тем временем, выздоровление или же стойкая ремиссия хронического простатита наступает только у 30-35% больных, которые получают традиционное лечение. В патогенезе хронического простатита важное место занимают мембранопатологические процессы, которые обусловлены активацией перекисного окисления липидов и нарушением состояния антиоксидантной системы защиты. У больных с хроническим простатитом и доброкачественной гиперплазией простаты наблюдается существенное усиление процессов липопероксидации на фоне сниженной функциональной способности антиоксидантных систем защиты организма. Поэтому равновесие в оксидантно-антиоксидантной системе является важным звеном в поддержании гомеостаза и, в частности, при патологии предстательной железы, предопределяет включение в комплексное лечение средств антиоксидантного действия. Перспективным можно считать применение препаратов с высоким содержанием биофлавоноидов и антиоксидантных витаминов. Включение в комплексное лечение таких пациентов препарата Кверцетина приводит к сокращению сроков нормализации клинико-лабораторных проявлений заболевания благодаря восстановлению равновесия между перекисным окислением липидов и состоянием антиоксидантной системы защиты Despite the successes achieved in the diagnosis and treatment, to date, the problem of chronic progression in patients with benign hyperplasia of the growth continues to remain relevant. Meanwhile, recovery or persistent remission of chronic prostatitis occurs only in 30-35% of patients who receive traditional treatment. In the pathogenesis of chronic prostatitis, membrane-pathological processes take an important place, which are caused by the activation of lipid peroxidation and impaired state of the antioxidant defense system. In patients with chronic prostate and benign hyperplasia, a significant increase in lipoperoxidation processes is observed against the background of a reduced functional ability of antioxidant defense systems. Therefore, the equilibrium in the oxidant-antioxidant system is an important link in the maintenance of homeostasis and, in particular, with the pathology of the prostate gland, allows the inclusion of antioxidant drugs in the complex treatment. The use of drugs with a high content of bioflavonoids and antioxidant vitamins can be considered promising. The inclusion of such patients in the complex treatment of the drug Quercetin leads to a reduction in the normalization period of the clinical and laboratory manifestations of the disease due to the restoration of the equilibrium between the peroxidation of the lipid peroxidation system and the state of lipid peroxidation

Indicators of the antioxidant system in the long-term period after acute mercury nitrate poisoning in the experiment

2020 ◽  
pp. 36-41
Author(s):  
E. G. Batotsyrenova ◽  
O. A. Vakunenkova ◽  
E. A. Zolotoverkhaya ◽  
V. A. Kashuro ◽  
T. A. Kostrova ◽  
...  
Keyword(s):  
Antioxidant System ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Antioxidant Defense System ◽  
Mercury Content ◽  
The State ◽  
Cytotoxic Effects ◽  
Entire Study ◽  
Mercury Nitrate

The article presents experimental data on the state of the antioxidant system in red blood cells of white outbred rats 1 and 3 months after acute mercury nitrate poisoning with a semilethal dose. It has been established that this form of intoxication is accompanied by pronounced changes in the state of the antioxidant defense system in erythrocytes of poisoned animals (a decrease in the concentration of reduced glutathione, a decrease in the activity of superoxide dismutase and glutathione peroxidase, and an increase in the concentration of lipid peroxidation products).It has been shown that the mercury content in the blood of experimental animals remains elevated during the entire study period.The results obtained indicate the importance of impaired functioning of the antioxidant system in the implementation of long-term consequences of acute mercury poisoning. The reasons for the occurrence of these biochemical shifts and their role in the development of the long-term cytotoxic effects of mercury nitrate are discussed.

scholarly journals Comparative Tissue Responses of Marine Mollusks on Seasonal Changes in the Northern Adriatic Sea

2021 ◽  
Vol 11 (6) ◽  
pp. 2874
Author(s):  
Natalija Topić Popović ◽  
Martina Krbavčić ◽  
Josip Barišić ◽  
Blanka Beer Ljubić ◽  
Ivančica Strunjak-Perović ◽  
...  
Keyword(s):  
Stress Responses ◽  
Seasonal Variations ◽  
Antioxidant Defense ◽  
Winter Season ◽  
Northern Adriatic ◽  
Gonadal Maturation ◽  
Marine Mollusks ◽  
Significant Difference ◽  
Tissue Responses ◽  
Antioxidant Defense Systems

In the shallow Northern Adriatic, marine mollusks are affected by bottom trawling and seafood disturbance. Seasonal oscillations of oceanographic factors additionally influence their physiology, stress responses and survival. Tissue responses to seasonal variations in green ormer (Haliotis tuberculata L.) and Mediterranean scallop (Pecten jacobaeus L.) in the Northern Adriatic have not been reported. Hence, their biochemical and antioxidant defense properties over seasons were studied and the microanatomical structure of their tissue was correlated with function. Histological analysis of gonads revealed two peaks of gonadal maturation and spawning during the spring/summer period and winter season for scallops, and one peak during the fall for ormers. The gonadal maturation of both species was correlated with their seasonal variations of metabolic demands and antioxidant capacity. The lipid vacuoles of tubuloacinar terminations in the digestive gland differed between the two species; in scallop they are several-fold larger in size and number. Low temperatures in winter contributed to a decline in enzymatic antioxidant defense in scallop tissues, having lower superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity, and higher concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant status (TAS). In ormers, winter induced lower TAS, TBARS, SOD and GPx concentrations. The significant difference of winter TAS and TBARS levels between ormers and scallops was correlated with variations in their reproductive cycles, as well as in antioxidant defense systems. The most important factor for stress-related parameters for both species in this work was found to be the season-induced temperature change.

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