scholarly journals Growth and development of bees using organic acids and probiotics

Author(s):  
I. V. Dmitruk ◽  
S. M. Suhovuha

The second record number of sealed brood, which took place on May 15 showed that consumption of succinic acid pergi helped increase the number of brood grown by 11.2% (P <0.001) and in the experimental group. Feeding bees use citric acid positively affects the amount of brood in the second experimental group. Compared with controls, the number of brood is higher by 7.5% (P < 0.001). Use of feeding bees III experimental group of lactic acid bacteria has increased the brood at 10.0% (P < 0.001). May 27 The difference between the number of counted squares and the control and experimental group is 16.3% (P <0.001), the second experimental group 11.5% (P < 0.001), the third experimental group 13.4% (P < 0.001). In the period, which fell on June 8 development of bee colonies increased, so the difference between the number of counted squares and the control and development of 22.2% (P < 0.001), the second experimental group 17.8% (P < 0.001), the third research group 18.9% (P < 0.001). June 20, recorded the best performance of the experimental group, the difference between the number of counted squares and the control and experimental group is 23.1% (P < 0.001), the second experimental group 18.3% (P < 0.001), the third experimental group 19.3 % (P < 0.001). Compared with the rate on May 3 yaytsenosnosti intensity oviposition of the uterus and research group for 27 May increased by 62.5%. Consumption pergi bees II nd experimental group caused an increase yaytsenosnosti cancer – by 56.3%, the third second experimental group – by 58.2%. Compared to control subjects in increasing the number of families found grown brood on 18,2 –23,1% (P <0.001).

2020 ◽  
Vol 22 (100) ◽  
pp. 23-27
Author(s):  
D. V. Kytaieva ◽  
R. V. Petrov

The specific microclimate of poultry premises contributes to the accumulation of microflora, which may contain pathogenic and opportunistic microorganisms. The concentration of microorganisms in poultry houses increases over time and reduces the resistance of the bird. Researchers have reported very high growth rates of mesophilic aerobic microorganisms, which include most pathogenic bacteria, namely: gram-positive cocci (staphylococci, enterococci) and gram-negative bacteria of the family Enterobacteriaceae (Escherichia coli, Salmonella spp., Salmonella spp.). Proteus spp., Klebsiella spp., and Pseudomonas spp. The goal of our research was to investigate and compare the effectiveness of two probiotics for turkeys, one of which contains recombinant strains of lactic acid microorganisms of intestinal intestinal symbionts, and the other based on a mixture of biologically active substances based on natural components of organo-mineral origin. The task of our work: 1. To study the microflora of the gastrointestinal tract during the use of probiotics after the use of antiparasitic drugs in nematode invasion of turkeys. 2. Compare the growth of young animals with the use of probiotics and provide suggestions on the prospects for the most effective use of probiotics in the cultivation of turkeys. 3. Investigate changes in the physicochemical properties of turkey meat when using probiotics and compare them with controls. To conduct the experiment, three experimental groups of turkeys cross Hybrid converter, 14 days old, each with 10 heads. The first and second experimental groups were given an antiparasitic drug containing levamisole hydrochloride at a dose of 5 ml per 5 liters of water once. After that, probiotics were used for each experimental group of birds. Two types of probiotics were used in the experiments: the first contains recombinant strains of lactic acid microorganisms of intestinal intestinal symbionts: Bifidobacterium bifidum, Bacillus thermophilus, Bacillus coagulance, Bacillus subtilis. Has antimicrobial, antiviral and antioxidant activity; the second preparation contains a mixture of biologically active substances, based on natural ingredients of organo-mineral origin, which includes organic substances – fulvic and humic acids. The third group (control) used only the above antiparasitic drug. After the experiment, at the age of 78 days, poultry was slaughtered and studies of the effect of probiotics on weight, physicochemical parameters of meat. When testing turkeys for helminths. As a result of research, helminths were identified, which were attributed to the causative agent of heterakidosis - Heeterakis gallibarum. After that, it was decided to carry out deworming with levamisole hydrochloride. After a course of treatment, it was found that no deaths were observed in the first experimental group; in the second experimental group, one head died, and in the third control group, which did not use probiotics, the death was two heads. The use of probiotics due to competition reduces the level of total bacterial contamination and coliform bacteria in the experimental groups. And the level of lactic acid bacteria in the experimental groups was higher than in the control. The use of probiotics had a positive effect on weight gain of experimental turkeys, compared with the control group, where probiotics were not used. The difference in the first experimental group compared with the control averaged 618 g, and in the second group – 198 g. Post-mortem examination of turkey carcasses showed that the indicators of the experimental and control groups differ. In terms of external organoleptic characteristics, the meat obtained from the slaughter of experimental birds does not differ from the meat of poultry in the control group. Conclusions and prospects for further research. The use of probiotics based on lactic acid bacteria proved to be the most effective and ensured the complete preservation of livestock. Due to competition, it reduced the level of coliform bacteria by an average of 6.67 % in the experimental groups; the level of lactic acid bacteria in the experimental groups was 4.89 % higher than in the control. The use of probiotics increased the weight gain of poultry by 3.28 %. No significant effect on the organoleptic and physicochemical properties of meat was observed.In the future it is planned to study the microflora of turkey slaughter products.


2000 ◽  
Vol 67 (1) ◽  
pp. 83-90 ◽  
Author(s):  
FERNANDA FONSECA ◽  
CATHERINE BÉAL ◽  
GEORGES CORRIEU

We have developed a method to quantify the resistance to freezing and frozen storage of lactic acid starters, based on measuring the time necessary to reach the maximum acidification rate in milk (tm) using the Cinac system. Depending on the operating conditions, tm increased during the freezing step and storage. The loss of acidification activity during freezing was quantified by the difference (Δtm) between the tm values of the concentrated cell suspension before and after freezing. During storage at −20 °C, linear relationships between tm and the storage time were established. Their slope, k, allowed the quantitation of the decrease in acidification activity during 9–14 weeks of frozen storage. The method was applied to determine the resistance to freezing and frozen storage of four strains of lactic acid bacteria and to quantify the cryoprotective effect of glycerol.


2019 ◽  
Vol 47 (1) ◽  
pp. 83
Author(s):  
Nelli Karolina Sitorus ◽  
Iesje Lukistyowati ◽  
Henni Syawal ◽  
Iskandar Putra

The aim of this research was to  determine the types of lactic acid bacteria from biofloc technology with the addition of mollase in red tilapia aquaculture containers and to determine the optimum growth of lactic acid bacteria at pH 2, 4, and 6, and also to observe the density of bacteria in the biofloc container water during maintenance. The methods used in this research are survey and experiments by taking flok from maintenance container and then identifying the lactic acid bacteria. The results of lactic acid bacteria inoculation obtained 16 isolates isolated from floc which can only grow at pH 4 and pH 6. Identification based on physical tests, chemical tests, and biochemical tests showed that the isolates were classified into Bacillus and Streptococcus. The amount of bacterial density water from containers of biofloc technology were given mollase during research reached the highest point in the third week, which averaged 109 CFU/mL.


Author(s):  
Mita Ekamelinda ◽  
I Wayan Suardana ◽  
Komang Januartha Putra Pinatih

Lactic acid bacteria are the type of bacteria that has benefits in food and health industries as a biopreservative, fermentative, or probiotics. Bali cattle are known as potential host for specific lactic acid bacteria. The aim of this study is to identify phenotypically lactic acid bacteria 9A isolated from bali cattle’s colon, that producing a substance which known has potency as antimicrobial. In this study, phenotypic identification included conventional method and API 50 CHL. The result of this study showed that lactic acid bacteria isolate 9A was Streptococcus sp., whereas identification by kit API 50 CHL showed isolate 9A as Lactobacillus fermentum with 83% identity. The difference between the results of conventional method and kit API 50 CHL, may indicate the difference in sensitivity and specificity of the two methods, hence it needs further confirmation.


1972 ◽  
Vol 35 (8) ◽  
pp. 489-495 ◽  
Author(s):  
H. s. Park ◽  
E. H. Marth

Cultured skimmilks containing Salmonella typhimurium were prepared at 21, 30, or 42 C using different species of lactic acid bacteria (0.25, 1.0, and 5% inoculum) either singly or in combinations. Several commercial cultures also were used. Cultured skimmilks were stored at 11 C and tested at 3-day intervals for numbers of viable salmonellae and lactic acid bacteria and for pH. Survival of S. typhimurium varied from 6 to 9 days and from 3 to 6 days in milks cultured with 0.25% Streptococcus lactis at 21 and 30 C, respectively. Increasing the inoculum to 1% with incubation at 30 C yielded a product no more detrimental to S. typhimurium than when the lower inoculum was used at 30 C. Survival of S. typhimurium always exceeded 9 days when S. cremoris was used to make cultured skimmilks. Products made with commercial mixed cultures composed of S. lactis and S. cremoris allowed S. typhimurium to survive for periods intermediate between the extremes observed when pure cultures were used. Skimmilks cultured with Streptococcus diacetilactis and Leuconostoc citrovorum, even when skimmilks cultured with the latter organism were acidified with citric acid and incubated further, were essentially without effect on survival of S. typhimurium during refrigerated storage. Use of Streptococcus thermophilus (1% culture, 42 C incubation) yielded cultured skimmilks that were most detrimental to survival of salmonellae, whereas skimmilks fermented with Lactobacillus bulgaricus permitted survival of low numbers of salmonellae beyond 9 days. Milks cultured at 42 C with a 5% inoculum of S. thermophilus mixed with L. bulgaricus or Lactobacillus helveticus were free of viable salmonellae before the incubation was complete. Salmonellae grown in skimmilk at 21 C without a lactic culture were more resistant to inactivation during refrigerated storage than was S. typhimurium grown at 30 or 42 C.


2020 ◽  
Vol 19 (1) ◽  
pp. 744-752
Author(s):  
Xuxiong Tao ◽  
Sifan Chen ◽  
Jie Zhao ◽  
Siran Wang ◽  
Zhihao Dong ◽  
...  

2011 ◽  
Vol 77 (18) ◽  
pp. 6694-6698 ◽  
Author(s):  
Timothy Lefeber ◽  
Maarten Janssens ◽  
Frédéric Moens ◽  
William Gobert ◽  
Luc De Vuyst

ABSTRACTAmong various lactic acid bacterial strains tested, cocoa-specific strains ofLactobacillus fermentumwere best adapted to the cocoa pulp ecosystem. They fermented glucose to lactic acid and acetic acid, reduced fructose to mannitol, and converted citric acid into lactic acid and 2,3-butanediol.


2021 ◽  
Vol 908 (1) ◽  
pp. 012006
Author(s):  
N S Lyakhovchenko ◽  
I A Nikishin ◽  
E D Gubina ◽  
D A Pribylov ◽  
V Y Senchenkov ◽  
...  

Abstract A study of antifungal properties of violacein-forming strain Janthinobacterium sp. B-3515 as well as its secondary metabolite, violacein, against Alternaria brassicicola F-1864 is presented. Regardless of the presence of bacteria, mycelium growth in the first two days proceeded at the same rate. The effect of the bacterial strain was manifested after the third day of incubation. In general, during co-culture, the bacterial strain statistically significantly reduced the average growth of the mycelium of the mould fungus by 10%. The average growth of A. brassicicola F-1864 was decreased in the presence of an aqueous solution of violacein in the nutrient medium (1%, 3%, and 5%). The pigment in 5% concentration had the greatest effect, as the difference between the average growth of the control group and the experimental group was 18%. The mycostatic activity of bacteria of genus Janthinobacterium and violacein against mould fungus Alternaria brassicicola F-1864 was shown for the first time.


Sign in / Sign up

Export Citation Format

Share Document