IDENTIFICATION OF LACTIC ACID BACTERIA FROM BIOFLOK TECHNOLOGY WHICH HAS BEEN GAVE MOLLASES ON RED TILAPIA (Oreochromis sp.) AQUACULTURE

The aim of this research was to determine the types of lactic acid bacteria from biofloc technology with the addition of mollase in red tilapia aquaculture containers and to determine the optimum growth of lactic acid bacteria at pH 2, 4, and 6, and also to observe the density of bacteria in the biofloc container water during maintenance. The methods used in this research are survey and experiments by taking flok from maintenance container and then identifying the lactic acid bacteria. The results of lactic acid bacteria inoculation obtained 16 isolates isolated from floc which can only grow at pH 4 and pH 6. Identification based on physical tests, chemical tests, and biochemical tests showed that the isolates were classified into Bacillus and Streptococcus. The amount of bacterial density water from containers of biofloc technology were given mollase during research reached the highest point in the third week, which averaged 109 CFU/mL.

SUSTAINABILITY ANALYSIS OF MANGROVE ECOSYSTEMSIN SUNGAI BELA VILLAGE, INDRAGIRI HILIR REGENCY

This study aims to determine the level of sustainability of mangrove ecosystems in Sungai Bela Village. The method used in this study is a survey method with data analysis using the Multi Dimensional Scaling (MDS). The results showed that the index value of the sustainability of mangrove ecosystems showed an ecological dimension(61.42) with RMS (2.28), social, economic and cultural dimensions (50.91) with RMS (2.51), legal and institutional dimensions (61.91) with RMS (1.79).Several factors that affect the sustainability of mangrove ecosystems such as:(1) mangrove species diversity;(2) density of mangrove ecosystems;(3) content of sediment organic matter;(4) marketing of fishery products;(5) mangrove dependence on livelihoods;(6) level of community knowledge;(7) the role of community leaders;(8) level of community compliance and (9) community participation.It can be concluded that the mangrove ecosystem in Sungai Bela Village with a sufficiently continuous status.

MORPHOMETRIC AND GROWTH PATTERNS OF THE BLOOD CLAM (Anadara granosa) FROM THE BAGAN SIAPI-API COASTAL AREA ROKAN HILIR

This study was conducted in the intertidal zone in the waters of Bagan Siapi-api Rokan Hilir Regency in May-June 2018. This study aims to examine morphometrics and growth patterns in blood clams (Anadara granosa) in Bagan Siapi-api waters. A total of 100 samples (23.89-50.52 mm shell length and 3.85-18.16 grams total weight) were analyzed. From the results of observations it was found that the number of male and female ribs was 8-20, and there were 3 differences in morphometric characteristics between male and female, namely male blood clams longer than females, while female blood shells were wider and thicker than males. Furthermore, the growth pattern of A. granosa was negative allometric, with male b values 1.46 and females 0.74.

CHARACTERISTICS OF FISH BONE FLOURS QUALITY FROM DIFFERENT TYPES OF FISH (Pangasius sp., Clarias sp., Paraplotosus sp.)

This study aimed to utilize the fish bone waste from different types of fish, Pangasius sp., Clarias sp., Paraplotosus sp. as a high calcium of bone flour and determine the quality characteristics of fish bone flour which was produced. The method used was a non-factorial Completely Randomized Design (CRD), with different types of fish bones as treatments which consisting of 3 levels: Pangasius sp. bone (Tp), Clarias sp. bone (Tl), and Paraplotosus sp. bone (Ts) with 3 replications and 9 units of experimental units. The parameters tested were organoleptic, proximate analysis, calcium, phosphorus and whiteness degrees. The results showed that the Pangasius sp. bone flour (Tp) was the best treatment with characteristics of brilliant appearance, the distinctive aroma of fish was still felt, smooth and dry texture, as well as whiteness degrees that the best treatment in walking catfish bone flour with value 81.30%, referred to whiteness degrees of flour, it is below the range of 80-90%. Meanwhile the best proximate value was obtained in Paraplotosus sp. bone treatment (Ts) with moisture, ash, fat, protein, carbohydrate, calcium and phosphorus content was 5.71%, 54.60%, 2.57%, 25.21%, 10.83%, 38.4%, and 33.9%.

THE POTENCY OF LUBUK LARANGAN JORONG IKAN BANYAK AS LOCAL WISDOM BASED ECOTOURISM AREA

This study aims to understand the local wisdom and the feasibility of the area as an ecological tourism object has been carried out from February to March 2018. The method used in this study is the survey method. Water quality data collection was carried out four times (once/two weeks), while questionnaires were distributed prior to environmental data collection. Water samples were taken from three stations, while local wisdom data were collected through questionnaires. Results shown that the community used to maintain the Jorong Ikan Banyak ecosystem by applying regulations for fish capture. Water quality parameter values of the Lubuk Larangan are as follows : temperature was 22.5-230c; water depth was 71.75-120.25 cm; current speed was 0.25-0.42 m/s; dissolved oxygen was 4.6-4.8 mg/l and pH was 6. The tourism potential element scores of the Lubuk Larangan were as follows: Attractiveness 1,095; Accessibility 537.5; Accommodations 45 and Facilities 240, total score 1,917.5. This score indicates that the Lubuk Larangan Jorong Ikan Banyak is suitable for tourism object.

THE EFFECTS OF PHOTOPERIOD MANIPULATION ON MORPHO-ANATOMY AND GROWTH OF Clarias gariepinus

Changing in photoperiod duration will affects the physiology of fish in general. A study aims to understand the effects of photoperiod manipulation towards C. gariepinus morpho-anatomy and growth was conducted on March–May 2018. The fishes were reared in fiber tanks (140 x 60 x 40 cm) that was filled with water and completed with a circulatory system. Treatments applied were Control (natural photoperiod), 24G (24 hours dark), 18G6T (18 hours dark and 6 hours light), and 6G18T (6 hours dark and 18 hours light). The fishes were sampled once/14 days, 4 fishes/ aquarium/sampling. Parameters measured were length-weight relationship, condition factor, hepatosomatic index (HSI), viscerasomatic index (VSI), absolute length and weight, food conversion ratio (FCR), gonad development and body protein/fat. Results shown that photoperiod manipulation affects C. gariepinus morpho-anatomy and growth. The highest growth parameters and morpho-anotomy index were found in fish reared in 18G6T. By the end of the experiment, the b value of length-weight relationship was 3.3205, absolute weight and length were 86.70 g and 17.23 cm, condition factor was 0.84, VSI was 25.31%, HSI was 11.42%, FCR was 1.43, and body protein and fat was 48.40% and 50.38%. In the fish reared in 24G treatment, b was 1.9262, 52.13 g BW and 14.19 cm SL, condition factor was 0.80, VSI was 23.30%, HSI was 9.38%, FCR was 2.47, and body protein and fat was 48.08% and 49.53%. In the fish reared in 6G18T, b was 2.6903,49.45 g BW and 13.79 cm SL, condition factor was 0.79, VSI was 23.30%, HSI was 6.80%, FCR was 1.91, and body protein and fat was 46.64% and 49.00%. Fish reared in natural photoperiod (control) shown the lowest results b was 2.0418, 51.68 g BW and 14.21 cm SL, condition factor was 0.79, VSI was 19.58%, HSI was 6.96%, FCR was 2.20, and body protein and fat was 43.95% and 47.77%.Early developing gonad (1st maturity stage) was only found in fish reared in 18G6T. Based on data obtained it can be concluded that the photoperiod manipulation affect the morpho-anatomy and growth of C. gariepinus.

CHARACTERISTICS AND EFFECTIVENESS OF CHITOSAN ORININATED FROM THE CARAPACE OF T. ANOMALA SHRIMP AS AN ANTIBACTERIAL COMPOUND

This study aims to investigate the inhibition zones of the chitosan against Pseudomonas aeruginosa. Carapace from T. anomala is used as raw material of chitosan. There were 5 treatments used, namely positive control (K0+), negative control (K0-), 5% chitosan (K1), 7% chitosan (K2) and 9% (K3) chitosan. The carapace was taken and then washed, dried, refined, demineralization, de-preoteinated, deasitelation and was tested using double layer method, discs and well diffusion. The proximate carapace test results were as follows, water content was 68.18 % gross wight (gw), moisture content 11.77 % dry wight (dw) protein content, 2.35 %dw fat content, 84.43 %dw ash content and carbohydrate content was 1.45 %dw. The chitosan of T. anomala characteristics were yellowish white flakes, odorless and tasteless with yield value, moisture content, ash content, mineral content (Ca, Fe, K, Na, P) and the degree of deacetylation 27.51 %; 7.31 %gw; 3.40 %dw; 12.17 mg/L; 4.90 mg/L; 3.40 mg/L; 5.49 mg/L; 0.27 mg/L; and 73.43 % respectively , Results on antimicrobial sensivity tests using double layer, disk blank and well diffusion. Shown clear zone, 13.87 mm, 11.34 mm and 14.62 mm. these values indicate that the antimicrobial effect of the chitosan is strong.

UTILIZATION OF SAMBUNG NYAWA LEAF EXTRACTS Gynura procumbens (Lour) Merr. FOR TREATMENT OF Vibrio alginolyticus IN TIGER GROUPER (Epinephelus fuscoguttatus Forsskal, 1775)

Tiger Grouper is one of the sea water fish commodities that is quite popular with the community and has a high economic value. The problem faced by farmers is the attack of Vibriosis, one of which is caused by the Vibrio alginolyticus bacteria. The use of synthetic antibiotics has been widely used but has many adverse effects, so it needs new alternatives for the treatment of Vibriosis disease. One of them is by using the extract of lifelong leaf extract. Life-sustaining plants (Gynura procumbens) contain secondary metabolites such as flavonoids, tannins, and antibacterial saponins. This study aims to determine the best dosage of lifelong leaf extract for the treatment of Vibriosis disease in tiger grouper. The study was conducted in two stages, namely in vitro and in vivo. Before the fish were treated with feed that had been given a sambung deca leaf extract, the fish were challenged using Vibrio alginolyticus with a density of 108 CFU / mL as much as 0.1 mL / head and then fed with treatment and maintained for 21 days. The results of the in vitro study showed that the life of sambung leaf extract at a dose of 700 ppm had a broad inhibitory effect on V. alginolyticus, which amounted to 10.47 mm compared to other treatments. Whereas when continued for in vivo testing, a dose of 350 ppm in general has been applied for the treatment of attacks of Vibrio alginolyticus in tiger grouper.

THE EFFECT OF DIFFERENT KIND OF CITRUS ON THE QUALITY OF JELAWAT FISH (Leptobarbus hoevenii) NANIURA

This study aimed to know the effect of the using of different citrus on the quality of hoven's carp fish naniura and to determine the other kind of citrus to substitute the jungga (Citrus jambhiri) in naniura processing. The research method used was experimental composed as a non-factorial completely randomized design (CRD). The treatment conducted was the using of different kinds of citrus (jungga, lime, lemon) in the processing of naniura. The results showed that the different citrus used was significantly affecting the organoleptic values (appearance, odor, taste, and texture), pH, water holding capacity, total plate number of bacteria, and a total of lactic acid bacteria. The best treatment is the use of lime (Citrus aurantifolia) with the characteristics of the appearance yellow-orange color, quite bright, and not pale; the odor was not smelling fishy but smelling characteristically naniura; the taste was sour and spicy, and the texture was soft and not hard. Naniura used of lime has a pH value of 4.8, water holding capacity 53.2, total plate number 3.6 x 103 Cfu/g, and lactic acid bacteria 4.3 x 105 Cfu/g

CHEMICAL AND SENSORY CHARACTERISTICS OF CARP MEAT (Osphronemus goramy) FROM DIFFERENT SIZES

This research aims to evaluate and compare of proximate composition, amino acid and fatty acid of carp (Osphronemus goramy) from different sizes: small (400-500 gr), medium (600 – 800 g), and large (900-1100 g). 27 samples of carp were obtained from a cage in Gurami village, Kampar. The edible portion, processing waste, water holding capacity, sensory quality, proximate, amino acid and fatty acid were evaluated. The result showed that appearance, odor, edible portion, processing waste were not different between fish sizes. The highest taste and texture were found in large size carp (8.5 and 8.1), then followed by medium (8.3 and 7.9), and small (7.6 and 7.8), respectively. The highest water holding capacity was found in medium size carp (32.4%) then large (28.3%) and small (26.0%). The result of proximate analysis of the highest water and ash content were found in small size carp (75.55% and 1.05%), then followed by medium (74.20% and 0.90%) and large (7.69% and 0.88%). The protein content of large size carp (20.88%) was higher than medium (18.70%) and small (18.36%). The fat content of large size carp (2.70%) was higher than medium (2.40%) and small (2.29%). The highest amino acid was found in large size carp (69.25%) followed by medium (56.38%) and small (49.86%). The highest fatty acid was found in large size carp (99.55%) followed by small (97.63%) and medium (97.20%).