scholarly journals Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp.

2019 ◽  
Vol 37 (2) ◽  
pp. 112-119
Author(s):  
Cecilia Paola Randazzo ◽  
Andrea Matilde Ferri ◽  
Leonela Carabajal Paladino ◽  
Adriana Noemi Andres ◽  
Lorena Romina Ingala
Keyword(s):  
Genetic Variation ◽  
Molecular Markers ◽  
Ssr Markers ◽  
Transfer Rate ◽  
Genetic Improvement ◽  
Genomic Information ◽  
Livestock Breeding ◽  
Setaria Sphacelata ◽  
Forage Species ◽  
Improvement Programs

Setaria sphacelata and Trichloris crinita are subtropical forage species that are important for livestock breeding in Argentina. Genomic information is scarce for these species, and there are no molecular markers designed for them; this limits the development of genetic improvement programs. We performed a cross-species transfer of SSR markers from several Poaceae species. In S. sphacelata, 8 SSR markers were transferred from Setaria italica (40% transfer rate), exhibiting 83% polymorphism. Kazungula, Splenda and Narok cultivars were genetically differentiated and the experimental material “Selección INTA” was separated from Narok, from which it was derived. For T. crinita, 19 microsatellites were transferred from 5 Poaceae species (7.3% transfer rate), with 69% polymorphism. The results obtained in this study show the potential of the transferred SSR markers for assessing genetic variation and for expanding the genetic resources available for these species.

scholarly journals Population structure and genetic diversity analysis in Sali (Oryza sativa) rice germplasm of Assam using microsatellite markers

2021 ◽  
Vol 58 (2) ◽  
pp. 279-286
Author(s):  
Sandhani Saikia ◽  
Pratap Jyoti Handique ◽  
Mahendra K Modi
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Genetic Improvement ◽  
Crop Improvement ◽  
Mean Value ◽  
Diversity Analysis ◽  
Rice Cultivars ◽  
Improvement Program ◽  
Information Index ◽  
Improvement Programs

Genetic diversity is the source of novel allelic combinations that can be efficiently utilized in any crop improvement program. To facilitate future crop improvement programs in rice, a study was designed to identify the underlying genetic variations in the Sali rice germplasms of Assam using SSR markers. The 129 SSR markers that were used in the study amplified a total of 765 fragments with an average of 5.93 alleles per locus. The Shannon's Information Index was found to be in the range from 0.533 to 1.786. The Polymorphism Information Content (PIC) fell into the range from 0.304 to 0.691 with a mean value of 0.55. The overall FST value was found to be 0.519 that indicated the presence of genetic differentiation amongst the genotypes used in the study. The Sali population was divided into two clusters. The information obtained from the present study will facilitate the genetic improvement of Sali rice cultivars.

scholarly journals Characterization of EST-SSRs for European beech (Fagus sylvatica L.) and their transferability to Fagus orientalis Lipsky, Castanea dentata Bork., and Quercus rubra L.

2018 ◽  
Vol 67 (1) ◽  
pp. 127-132 ◽  
Author(s):  
Katrin Burger ◽  
Markus Müller ◽  
Oliver Gailing
Keyword(s):  
Genetic Variation ◽  
Molecular Markers ◽  
Ssr Markers ◽  
Population Genetic ◽  
Quercus Rubra ◽  
European Beech ◽  
Castanea Dentata ◽  
Fagus Orientalis Lipsky ◽  
Fagus Sylvatica L ◽  
Population Genetic Analyses

Abstract Due to ongoing climate change, forests are exposed to changing environmental conditions, such as increasing temperatures and lower precipitation, to which trees have to adapt. Successful adaptation to changing and variable environments requires sufficient genetic variation within tree populations. Knowledge of the genetic variation of trees is therefore essential, as it provides information for the long-term conservation, stability and productivity of forests. The genetic variation of a species can be analysed with molecular markers. Despite growing genomic and genetic resources for European beech (Fagus sylvatica L.), which is one of the economically and ecologically most important forest tree species in Central Europe, the number of molecular markers for population genetic analyses is still limited. Therefore, the aim of the work is the development of new EST-SSR markers for this species. A total of 72 DNA samples of European beech from three widely separated regions in Germany were used to test 41 primers for variation and polymorphism, 35 of which were originally developed for American beech (Fagus grandifolia Ehrh.) and 6 for red oak (Quercus rubra L.). Fifteen of the primers were polymorphic, 13 monomorphic and 13 did not amplify. In addition, the transferability of the markers was successfully tested in the related species Castanea dentata Bork., Fagus orientalis Lipsky and Q. rubra. The EST-SSR markers tested in this study will be useful for future population genetic analyses and extend the set of available markers in European beech.

Genetic variation and population structure of Rosa roxburghii by EST-based and genomic SSR markers

2020 ◽  
Vol 52 (4) ◽  
Author(s):  
Min Lu ◽  
Huaishan Zhang ◽  
Huaming An ◽  
Wen Zhou
Keyword(s):  
Population Structure ◽  
Genetic Variation ◽  
Ssr Markers ◽  
Genomic Ssr ◽  
Rosa Roxburghii

scholarly journals Application of Tissue Culture in Plant Reproduction

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 342
Author(s):  
Elena Corredoira ◽  
Rita L. Costa
Keyword(s):  
Tissue Culture ◽  
Genetic Improvement ◽  
Plant Reproduction ◽  
Climatic Conditions ◽  
Improvement Programs

The increasing degradation of forests, together with a higher demand for wood and fruit, has led to the need for more efficient trees adapted to the current climatic conditions and, thus, to the need for genetic improvement programs [...]

scholarly journals Genotyping of Peach and Nectarine Cultivars with SSR and SRAP Molecular Markers

2004 ◽  
Vol 129 (2) ◽  
pp. 204-210 ◽  
Author(s):  
Riaz Ahmad ◽  
Dan Potter ◽  
Stephen M. Southwick
Keyword(s):  
Molecular Markers ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Sweet Cherry ◽  
Prunus Persica ◽  
Sequence Repeat ◽  
Srap Markers ◽  
Upgma Cluster Analysis ◽  
Commercially Important ◽  
Simple Sequence

Simple sequence repeat (SSR) and sequence related amplified polymorphism (SRAP) molecular markers were evaluated for detecting intraspecific variation in 38 commercially important peach and nectarine (Prunus persica) cultivars. Out of the 20 SSR primer pairs 17 were previously developed in sweet cherry and three in peach. The number of putative alleles revealed by SSR primer pairs ranged from one to five showing a low level of genetic variability among these cultivars. The average number of alleles per locus was 2.2. About 76% of cherry primers produced amplification products in peach and nectarine, showing a congeneric relationship within Prunus species. Only nine cultivars out of the 38 cultivars could be uniquely identified by the SSR markers. For SRAP, the number of fragments produced was highly variable, ranging from 10 to 33 with an average of 21.8 per primer combination. Ten primer combinations resulted in 49 polymorphic fragments in this closely related set of peaches and nectarines. Thirty out of the 38 peach and nectarine cultivars were identified by unique SRAP fingerprints. UPGMA Cluster analysis based on the SSR and SRAP polymorphic fragments was performed; the relationships inferred are discussed with reference to the pomological characteristics and pedigree of these cultivars. The results indicated that SSR and SRAP markers can be used to distinguish the genetically very close peach and nectarine cultivars as a complement to traditional pomological studies. However, for fingerprinting, SRAP markers appear to be much more effective, quicker and less expensive to develop than are SSR markers.

Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis

2012 ◽  
Vol 92 (6) ◽  
pp. 1121-1133 ◽  
Author(s):  
S. C. Debnath ◽  
Y. L. Siow ◽  
J. Petkau ◽  
D. An ◽  
N. V. Bykova
Keyword(s):  
Genetic Diversity ◽  
Antioxidant Activity ◽  
Molecular Markers ◽  
Molecular Techniques ◽  
Diversity Analysis ◽  
Full Potential ◽  
Genetic Diversity Analysis ◽  
Wide Range ◽  
Berry Crops ◽  
Improvement Programs

Debnath, S. C., Siow, Y. L., Petkau, J., An, D. and Bykova, N. V. 2012. Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis. Can. J. Plant Sci. 92: 1121–1133. An improved understanding of important roles of dietary fruits in maintaining human health has led to a dramatic increase of global berry crop production. Berry fruits contain relatively high levels of vitamin C, cellulose and pectin, and produce anthocyanins, which have important therapeutic values, including antitumor, antiulcer, antioxidant and anti-inflammatory activities. There is a need to develop reliable methods to identify berry germplasm and assess genetic diversity/relatedness for dietary properties in berry genotypes for practical breeding purposes through genotype selection in a breeding program for cultivar development, and proprietary-rights protection. The introduction of molecular biology techniques, such as DNA-based markers, allows direct comparison of different genetic materials independent of environmental influences. Significant progress has been made in diversity analysis of wild cranberry, lowbush blueberry, lingonberry and cloudberry germplasm, and in strawberry and raspberry cultivars and advanced breeding lines developed in Canada. Inter simple sequence repeat (ISSR) markers detected an adequate degree of polymorphism to differentiate among berry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in the current berry improvement programs. Although multiple factors affect antioxidant activity, a wide range of genetic diversity has been reported in wild and cultivated berry crops. Diversity analysis based on molecular markers did not agree with those from antioxidant activity. The paper also discusses the issues that still need to be addressed to utilize the full potential of molecular techniques including expressed sequence tag-polymerase chain reaction (EST-PCR) analysis to develop improved environment-friendly berry cultivars suited to the changing needs of growers and consumers.

scholarly journals Genetic variation within and between populations of a desert poplar (Populus euphratica) revealed by SSR markers

2011 ◽  
Vol 68 (6) ◽  
pp. 1143-1149 ◽  
Author(s):  
Juan Wang ◽  
Zhijun Li ◽  
Qiuhong Guo ◽  
Guangpeng Ren ◽  
Yuxia Wu
Keyword(s):  
Genetic Variation ◽  
Ssr Markers ◽  
Populus Euphratica

The systematic collection and characterisation of herbaceous forage species for recharge and discharge environments in southern Australia

2008 ◽  
Vol 48 (4) ◽  
pp. 397 ◽  
Author(s):  
S. J. Hughes ◽  
R. Snowball ◽  
K. F. M. Reed ◽  
B. Cohen ◽  
K. Gajda ◽  
...  
Keyword(s):  
Genetic Resource ◽  
Dryland Salinity ◽  
Diverse Range ◽  
Comprehensive Collection ◽  
Forage Species ◽  
Improvement Programs ◽  
Species Specific ◽  
New Germplasm ◽  
Herb Species ◽  
Systematic Collection

Australian temperate pasture Genetic Resource Centres (GRCs) co-ordinated a major program to introduce and screen herbaceous forage species. This program aims to expand the environments where such species could reduce recharge and manage discharge for the control of dryland salinity in southern Australia. The sustainability of agriculture, in Australia especially, depends on continued access to new germplasm to enable plant breeders to continue crop and forage improvement. The GRCs supported the selection and identification of promising new legume, grass and herb species as part of a national pasture evaluation program. In total, 671 species and 21 non-species-specific genera were identified as having potential to increase water use profitability of recharge lands and to improve the productivity of saline lands across a diverse range of agricultural environments in southern Australia. Through a series of activities, 201 of these species, representing legumes, herbs and grasses were identified as promising. These were then disseminated for evaluation in a range of environments across southern Australia. The progress of selected species was monitored and germplasm of the most promising 11 species and three leguminous genera was targeted for intensive acquisition and characterisation as the basis for selection and breeding. In addition to the identification and dissemination of promising species of immediate potential, a comprehensive collection of 544 native and exotic, wild and cultivated pasture species was conserved and is now available to service future plant improvement programs.

scholarly journals Hybrid identification and genetic variation of Elymus sibiricus hybrid populations using EST-SSR markers

Hereditas ◽  
2017 ◽  
Vol 154 (1) ◽  
Author(s):  
Xuhong Zhao ◽  
Junchao Zhang ◽  
Zongyu Zhang ◽  
Yanrong Wang ◽  
Wengang Xie
Keyword(s):  
Genetic Variation ◽  
Ssr Markers ◽  
Hybrid Identification ◽  
Elymus Sibiricus
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