Hongos asociados a la pudrición seca de tubérculos de ñame (Dioscorea rotundata Poir.) en Córdoba, Colombia

Rhizopus Oryzae ◽

Dioscorea Rotundata ◽

Neoscytalidium Dimidiatum

Introducción. La pudrición seca es una enfermedad que ha adquirido gran importancia en Colombia debido a que ocasiona la pérdida de los tubérculos del ñame (Dioscorea rotundata Poir.), tanto en campo como en almacenamiento, afecta los ingresos económicos y la productividad de los pequeños productores. Es escasa la información respecto a los agentes causales de esta enfermedad, por lo que no se tienen claras las estrategias para el manejo de esta problemática. Objetivo. Identificar y caracterizar hongos asociados a la pudrición seca de tubérculos de ñame. Materiales y métodos. Esta investigación se realizó entre marzo y octubre del año 2019, en la zona costanera del departamento de Córdoba en el Caribe colombiano. Se colectaron tubérculos de ñame en almacenamiento, debido a que los agricultores los conservan como semillas para próximas cosechas. En laboratorio se aislaron de las lesiones los hongos asociados, se caracterizaron y luego se identificaron a través de la amplificación de la región ITS (Internal Transcribed Spacer) del DNA ribosomal. Resultados. Se aislaron veintiocho crecimientos fúngicos asociados a la pudrición seca de los tubérculos del ñame, los cuales se agruparon en once morfotipos, de los cuales se identificaron diez especies, pertenecientes a nueve géneros fúngicos. Conclusión. Seis hongos podrían estar asociados a la pudrición seca de los tubérculos de ñame en la zona costanera de Colombia. Estos serían: Lasiodiplodia theobromae, Fusarium solani, Rhizopus oryzae, Neocosmospora rubicola, Neoscytalidium dimidiatum y Curvularia aeria.

Mycoflora associated with two species of Liliaceae in Bangladesh

Dhaka University Journal of Biological Sciences ◽

10.3329/dujbs.v22i1.46272 ◽

2013 ◽

Vol 22 (1) ◽

pp. 39-46

Author(s):

Shamim Shamsi ◽

Najmun Naher

Keyword(s):

Fusarium Solani ◽

Alternaria Alternata ◽

New Record ◽

Macrophomina Phaseolina ◽

Fungal Species ◽

Colletotrichum Orbiculare ◽

Colletotrichum Capsici ◽

Colletotrichum Dematium

A total of nine fungi were isolated from two ornamental angiosperms, namely Hemerocallis fulva L. and Pancratium verecundum Ant. belong to Liliaceae. Three species of fungi were found to be associated with the leaf of Hemerocallis fulva. The fungi were Colletotrichum capsici, (Syd.) Bull. & Bisby, Colletotrichum dematium (Pers. Ex Fr.) and Glomerella montana (Sacc.)v Arx & E. Muller. Six species of fungi, namely Alternaria alternata (Fries) Keissler, Colletotrichum orbiculare (Berk & Mont.) Arex., Curvularia clavata Jain, Fusarium solani (Mort.) Sacc., Lasiodiplodia theobromae (Pat.) Griffon and Maubol and Macrophomina phaseolina (Tassi) Goid were found to be associated with P. verecundum. Among the fungal species Glomerella montana is new record for Bangladesh. Dhaka Univ. J. Biol. Sci. 22(1): 39-46, 2013 (January)

Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants

Annals of Applied Biology ◽

10.1111/j.1744-7348.2012.00534.x ◽

2012 ◽

Vol 160 (3) ◽

pp. 217-224 ◽

Cited By ~ 2

Author(s):

C.R. Muniz ◽

F.C.O. Freire ◽

F.M.P. Viana ◽

J.E. Cardoso ◽

D. Correia ◽

...

Keyword(s):

Polyclonal Antibody ◽

Pcr Amplification ◽

Causal Agent ◽

Internal Transcribed Spacer Regions ◽

Specific Pcr ◽

Cashew Nut

Evaluation of Clove Essential Oil as a Mycobiocide Against Rhizopus stolonifer and Fusarium solani , Tuber Rot Causing Fungi in Yam (Dioscorea rotundata Poir.)

Journal of Phytopathology ◽

10.1111/jph.12468 ◽

2016 ◽

Vol 164 (7-8) ◽

pp. 433-440 ◽

Cited By ~ 11

Author(s):

Modeste Lambert Sameza ◽

Lile Christere Nguemnang Mabou ◽

Séverin Nguemezi Tchameni ◽

Marie Ampère Boat Bedine ◽

François Tchoumbougnang ◽

...

Keyword(s):

Essential Oil ◽

Fusarium Solani ◽

Rhizopus Stolonifer ◽

Dioscorea Rotundata ◽

Clove Essential Oil ◽

Tuber Rot

Fatal rhinocerebral mucormycosis in a patient with ulcerative colitis receiving azathioprine and corticosteroid

Current Medical Mycology ◽

10.18502/cmm.5.1.536 ◽

2019 ◽

Cited By ~ 2

Author(s):

Narges Najafi ◽

Firoozeh Kermani ◽

Nahid Gholinejad Ghadi ◽

Seyed Reza Aghili ◽

Zahra Seifi ◽

...

Keyword(s):

Ulcerative Colitis ◽

Adverse Effect ◽

Rhizopus Oryzae ◽

Culture Media ◽

Intestinal Bleeding ◽

High Dose ◽

Rhinocerebral Mucormycosis ◽

Polymerase Chain ◽

Extensive Necrosis

Background and Purpose: Rhinocerebral mucormycosis is a rare fatal fungal infectionwhich is on a growing trend, particularly among immunocompromised patients.Immunosuppressive drugs, including corticosteroids and antimetabolites, increase therisk of this infection. Herein, we reported the case of fulminant rhinocerebralmucormycosis in a patient with ulcerative colitis receiving azathioprine andcorticosteroid.Case report: A 58-year-old woman was admitted to the hospital in a state of coma withan extensive necrosis in her nose. She was afflicted with intestinal bleeding after 1month of fasting and was treated with azathioprine and a high dose of prednisolone forulcerative colitis 2 months prior to hospital admission. The direct microscopicexamination of the necrotic tissues of the paranasal sinuses showed several non-septatehyphae consistent with Mucorales. Culture media yielded Rhizopus species, which wasidentified as Rhizopus oryzae by internal transcribed spacer polymerase chain reactionsequencing. Despite the implementation of surgical and pharmaceutical (liposomalamphotericin B) treatments, the patient expired after 2 weeks of admission.Conclusion: The gastroenterologists should be aware of the adverse effect ofimmunosuppressive drugs they prescribe for the treatment of inflammatory boweldisease.

Potencial antifúngico de quitosanases extracelulares de Fusarium solani FA14 contra os fitopatógenos Aspergillus niger, Colletotrichum gloeosporioides e Lasiodiplodia theobromae

Scientia Plena ◽

10.14808/sci.plena.2016.126202 ◽

2016 ◽

Vol 12 (12) ◽

Author(s):

Samuel Alves Bezerra Neto ◽

Lorena Nogueira Frota Da Costa ◽

Erlândia Alves Magalhães ◽

Francisco Lucas Alves Batista ◽

Luiz Francisco Wemmenson Gonçalves Moura ◽

...

Keyword(s):

Aspergillus Niger ◽

Fusarium Solani ◽

Colletotrichum Gloeosporioides ◽

Penicillium Sp

O trabalho reporta o potencial antifúngico de quitosanases extracelulares do fungo Fusarium solani FA14 contra os fitopatógenos Aspergillus niger, Colletotrichum gloeosporioides e Lasiodiplodia theobromae. Inicialmente, a cepa foi submetida a ensaio de atividade quitosanase (AQ) e se mostrou ativa, com valor de AQ igual a 1,1 significantemente igual (p<0,05) ao do controle positivo, Penicillium sp. Posteriormente a cepa foi empregada como fonte de quitosanases extracelulares para avaliar o percentual de inibição do crescimento (PIC) dos fungos Aspergillus niger, Colletotrichum gloeosporioides, Lasiodiplodia theobromae. Como resultado, o extrato enzimático de F. solani FA14 se mostrou ativo contra todos os fungos desafiados, com valores de PIC variando de 21 a 64%, significantemente maiores (p<0,05) do que os dos extratos do Penicillium sp. O extrato enzimático do F. solani FA14 apresentou atividade antifúngica mais expressiva contra o fitopatógeno Lasiodiplodia theobromae, com valor de PIC igual a 64%, significantemente maior (p<0,05) do que os dos extratos do Penicillium sp. (PIC = 50%).

Activity of Posaconazole and Other Antifungal Agents against Mucorales Strains Identified by Sequencing of Internal Transcribed Spacers

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01467-08 ◽

2009 ◽

Vol 53 (4) ◽

pp. 1686-1689 ◽

Cited By ~ 48

Author(s):

Ana Alastruey-Izquierdo ◽

Maria Victoria Castelli ◽

Isabel Cuesta ◽

Araceli Monzon ◽

Manuel Cuenca-Estrella ◽

...

Keyword(s):

Antifungal Agents ◽

Rhizopus Oryzae ◽

Antifungal Susceptibility ◽

Active Agent ◽

Mucor Circinelloides ◽

Internal Transcribed Spacers ◽

Good Activity ◽

Rhizomucor Variabilis ◽

Susceptibility Profiles

ABSTRACT The antifungal susceptibility profiles of 77 clinical strains of Mucorales species, identified by internal transcribed spacer sequencing, were analyzed. MICs obtained at 24 and 48 h were compared. Amphotericin B was the most active agent against all isolates, except for Cunninghamella and Apophysomyces isolates. Posaconazole also showed good activity for all species but Cunninghamella bertholletiae. Voriconazole had no activity against any of the fungi tested. Terbinafine showed good activity, except for Rhizopus oryzae, Mucor circinelloides, and Rhizomucor variabilis isolates.

First report of rhinosinusitis caused by Neoscytalidium dimidiatum in Iran

Journal of Medical Microbiology ◽

10.1099/jmm.0.065292-0 ◽

2014 ◽

Vol 63 (7) ◽

pp. 1017-1019 ◽

Cited By ~ 15

Author(s):

Mandana Bakhshizadeh ◽

Hamid Reza Hashemian ◽

Mohammad Javad Najafzadeh ◽

Somayeh Dolatabadi ◽

Hossein Zarrinfar

Keyword(s):

Dna Sequencing ◽

Its Region ◽

First Report ◽

Neoscytalidium Dimidiatum ◽

Fungal Rhinosinusitis

The study describes isolation of Neoscytalidium dimidiatum from a case of eosinophilic fungal rhinosinusitis (EFRS). The isolate was identified by routine mycological methods and confirmed by DNA sequencing of the internal transcribed spacer (ITS) region of rDNA. To our knowledge, this is the first report of its kind from Iran.

Mogroside V-producing Endophytic Fungi Isolated from Siraitia grosvenorii

Planta Medica ◽

10.1055/a-1102-1168 ◽

2020 ◽

Vol 86 (13/14) ◽

pp. 983-987 ◽

Cited By ~ 1

Author(s):

Chen Bin ◽

Yu Fangming ◽

Jiang Zhi

Keyword(s):

Endophytic Fungi ◽

Fusarium Solani ◽

High Performance ◽

Host Plants ◽

Morphological Observation ◽

Its Sequence ◽

Active Components ◽

Siraitia Grosvenorii ◽

Stem Leaf

AbstractEndophytic fungi can produce beneficial active components during symbiosis with host plants. Fifteen purified endophytic fungal strains were isolated and screened from root, stem, leaf, and fruit of the Chinese medicine food homologous plant Siraitia grosvenorii. These strains were fermented, and then the fermented solution was extracted using acetonitrile. The extracts were analyzed by high performance liquid chromatography (HPLC) and mass spectrum (MS), using standard mogroside V as control. The results showed that strain LHG-F5 and strain LHG-L4 could produce mogroside V with output of 3.814 µg/mL and 2.675 µg/mL, respectively. By morphological observation together with similarity of internal transcribed spacer (ITS) sequence by Clustan X method, LHG-F5 and LHG-L4 were classified as Diaporthe angelica and Fusarium solani, respectively.