scholarly journals Fatal rhinocerebral mucormycosis in a patient with ulcerative colitis receiving azathioprine and corticosteroid

2019 ◽  
Author(s):  
Narges Najafi ◽  
Firoozeh Kermani ◽  
Nahid Gholinejad Ghadi ◽  
Seyed Reza Aghili ◽  
Zahra Seifi ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Adverse Effect ◽  
Internal Transcribed Spacer ◽  
Rhizopus Oryzae ◽  
Culture Media ◽  
Intestinal Bleeding ◽  
High Dose ◽  
Rhinocerebral Mucormycosis ◽  
Polymerase Chain ◽  
Extensive Necrosis

Background and Purpose: Rhinocerebral mucormycosis is a rare fatal fungal infectionwhich is on a growing trend, particularly among immunocompromised patients.Immunosuppressive drugs, including corticosteroids and antimetabolites, increase therisk of this infection. Herein, we reported the case of fulminant rhinocerebralmucormycosis in a patient with ulcerative colitis receiving azathioprine andcorticosteroid.Case report: A 58-year-old woman was admitted to the hospital in a state of coma withan extensive necrosis in her nose. She was afflicted with intestinal bleeding after 1month of fasting and was treated with azathioprine and a high dose of prednisolone forulcerative colitis 2 months prior to hospital admission. The direct microscopicexamination of the necrotic tissues of the paranasal sinuses showed several non-septatehyphae consistent with Mucorales. Culture media yielded Rhizopus species, which wasidentified as Rhizopus oryzae by internal transcribed spacer polymerase chain reactionsequencing. Despite the implementation of surgical and pharmaceutical (liposomalamphotericin B) treatments, the patient expired after 2 weeks of admission.Conclusion: The gastroenterologists should be aware of the adverse effect ofimmunosuppressive drugs they prescribe for the treatment of inflammatory boweldisease.

Recovery from rhinocerebral mucormycosis in a ketoacidotic diabetic patient: a case report

1993 ◽  
Vol 107 (3) ◽  
pp. 233-235 ◽  
Author(s):  
J. Kemper ◽  
E. J. Kuijper ◽  
P. G. B. Mirck ◽  
A. J. M. Balm
Keyword(s):  
Diabetes Mellitus ◽  
Rhizopus Oryzae ◽  
Insulin Dependent Diabetes Mellitus ◽  
Dependent Diabetes Mellitus ◽  
High Dose ◽  
Rhinocerebral Mucormycosis ◽  
Facial Swelling ◽  
Insulin Dependent ◽  
The Right ◽  
And Control

An 18-year-old woman with insulin-dependent diabetes mellitus developed an infection of the paranasal sinuses with Rhizopus oryzae resulting in facial swelling, hemiplegia and blindness of the right eye. The therapy of this rhinocerebral mucormycosis consisted of extensive surgical debridement, administration of high-dose amphotericin B, hyperbaric oxygen and control of the underlying predisposing diabetes mellitus. The patient eventually recovered with however, the loss of one eye.

Producing biodiesel from cottonseed oil using Rhizopus oryzae ATCC #34612 whole cell biocatalysts: Culture media and cultivation period optimization

2013 ◽  
Vol 17 (4) ◽  
pp. 331-336 ◽  
Author(s):  
Sneha Athalye ◽  
Ratna Sharma-Shivappa ◽  
Steven Peretti ◽  
Praveen Kolar ◽  
Jack P. Davis
Keyword(s):  
Rhizopus Oryzae ◽  
Culture Media ◽  
Cottonseed Oil ◽  
Whole Cell ◽  
Cultivation Period

scholarly journals Neuroleptic malignant syndrome following catatonia: Vigilance is the price of antipsychotic prescription

2017 ◽  
Vol 5 ◽  
pp. 2050313X1769599 ◽  
Author(s):  
Thomas J Reilly ◽  
Sean Cross ◽  
David M Taylor ◽  
Richard Haslam ◽  
Sophie C Tomlin ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Adverse Drug Reaction ◽  
Pulmonary Embolism ◽  
Neuroleptic Malignant Syndrome ◽  
Kidney Injury ◽  
Clinical Suspicion ◽  
Antipsychotic Treatment ◽  
High Dose ◽  
Intravenous Fluids ◽  
Life Threatening

Objectives: To describe a case of neuroleptic malignant syndrome following antipsychotic treatment of catatonia, highlighting the potentially serious complications of this rare adverse drug reaction. Methods: We present a case report of a patient who developed this syndrome with various sequelae. Results: The patient developed neuroleptic after being treated with lorazepam and olanzapine for catatonia. He subsequently developed the complications of rhabdomyolysis, acute kidney injury, pulmonary embolism, urinary retention and ileus. He received high-dose lorazepam, anticoagulation and intravenous fluids. Antipsychotic medication in the form of haloperidol was reinstated with no adverse effect, and he went on to make a full recovery. Conclusions: This case illustrates the potential life-threatening complications of neuroleptic malignant syndrome and the need for a low index of clinical suspicion. It also highlights the lack of evidence for treatment of catatonia, including the use of antipsychotics.

scholarly journals Antioxidant or Apoptosis Inhibitor Supplementation in Culture Media Improves Post-Thaw Recovery of Murine Spermatogonial Stem Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 754
Author(s):  
Sang-Eun Jung ◽  
Hui-Jo Oh ◽  
Jin-Seop Ahn ◽  
Yong-Hee Kim ◽  
Bang-Jin Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Functional Activity ◽  
Culture Media ◽  
Quantitative Polymerase Chain Reaction ◽  
Spermatogonial Stem Cells ◽  
Ros Generation ◽  
Apoptosis Assay ◽  
Polymerase Chain ◽  
Apoptosis Inhibitor

We postulated that supplementation of antioxidant or apoptosis inhibitor in post-thaw culture media of spermatogonial stem cells (SSCs) alleviates reactive oxygen species (ROS) generation and apoptosis. Our aim was to develop an effective culture media for improving post-thaw recovery of SSCs. To determine the efficacy of supplementation with hypotaurine (HTU), α-tocopherol (α-TCP), and Z-DEVD-FMK (ZDF), we assessed the relative proliferation rate and SSC functional activity and performed a ROS generation assay, apoptosis assay, and western blotting for determination of the Bax/Bcl-xL ratio, as well as immunocytochemistry and real-time quantitative polymerase chain reaction (RT-qPCR) for SSC characterization. The relative proliferation rates with HTU 400 μM (133.7 ± 3.2%), α-TCP 400 μM (158.9 ± 3.6%), and ZDF 200 μM (133.1 ± 7.6%) supplementation were higher than that in the DMSO control (100 ± 3.6%). ROS generation was reduced with α-TCP 400 μM (0.8-fold) supplementation in comparison with the control (1.0-fold). Early apoptosis and Bax/Bcl-xL were lower with α-TCP 400 μM (2.4 ± 0.4% and 0.5-fold) and ZDF 200 μM (1.8 ± 0.4% and 0.3-fold) supplementation in comparison with the control (5.3 ± 1.4% and 1.0-fold) with normal characterization and functional activity. Supplementation of post-thaw culture media with α-TCP 400 μM and ZDF 200 μM improved post-thaw recovery of frozen SSCs via protection from ROS generation and apoptosis after cryo-thawing.

Abstract 74: The Nox4 Inhibitor GKT137831, Prevents Aldosterone Production by Adipocytes and Protects Against Adipose Tissue Inflammation and Fibrosis in Obese Mice

Hypertension ◽  
2013 ◽  
Vol 62 (suppl_1) ◽  
Author(s):  
Sarah Even ◽  
Aurelie Nguyen Dinh Cat ◽  
Francisco J Rios ◽  
Antunes T Tayze ◽  
Ying He ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Culture Media ◽  
Inflammatory Marker ◽  
High Dose ◽  
Obese Mice ◽  
Cardiovascular Damage ◽  
Aldosterone Production ◽  
Inflammatory Phenotype ◽  
Sirius Red

Aldosterone (aldo) plays an important role in obesity-associated cardiovascular risk. We demonstrated that aldo is produced by adipocytes, an effect associated with increased generation of reactive oxygen species (ROS). These processes are exaggerated in obesity. The relationship between adipocyte aldosterone and ROS is unclear. We postulated that Nox4-derived ROS is important for aldo production in adipocytes and leads to a pro-inflammatory phenotype in obesity. Studies were performed in db/m (lean) and db/db (obese) mice, treated with low (20mg/kg/day) or high dose (60mg/kg/day) GKT137831 (GKT, Nox4 inhibitor, 16 weeks). Epididymal (EVAT) and perivascular (PVAT) fat were collected. Plasma and adipocyte aldo were measured by ELISA. Adipose tissue fibrosis was evaluated by picro Sirius red staining and inflammatory mediators by immunostudies. Body weight was increased in db/db mice (61.8g vs control 33.5g), with no effect of GKT. Epididymal adiposity was increased in db/db mice (0.098g vs. 0.067g, p<0.05). Plasma aldo levels in db/db (pg/mL: 518 vs. 272g) and aldo levels in culture media from db/db adipocytes were increased (pg/mL/μg RNA: 1964 vs. 388), p<0.05. All effects decreased by high dose GKT. In PVAT, CYP11B2 gene expression was increased in db/db (2.6±0.8 vs control 1.1±0.1, p<0.05), an effect blocked by Nox4 inhibition. Gene expression of adipocyte differentiation marker, AP2, was increased (3.5±1.1 vs control 1.4±0.4) while anti-inflammatory marker adiponectin was decreased (0.7±0.1 vs control 1.3±0.2, p<0.05)) in obese mice. GKT decreased AP2 levels. Adipocyte-derived TNFα was increased in db/db (4.9±1.8 vs control 1.6±0.6, p<0.05), an effect blocked by GKT. Pro-collagen I, marker of fibrosis, was increased in db/db mice (132±11 vs control 87±4, p<0.05). Sirius red staining was exaggerated in EVAT from db/db mice, and decreased by Nox4 inhibition. In conclusion, Nox4 plays a role in regulating adipocyte-derived aldosterone and promotes a pro-inflammatory and profibrotic adipose phenotype in obese db/db mice. These findings suggest that adipocyte Nox4 links hyperaldosteronism and inflammation/fibrosis in adiposity and as such may be a putative therapeutic target for obesity-associated cardiovascular damage.

A RARE ASSOCIATION OF CHRONIC INFLAMMATORY DEMYELINATING POLYNEUROPATHY WITH ULCERATIVE COLITIS

2021 ◽  
pp. 18-19
Author(s):  
Nilima K. Shah ◽  
Ankita Patel ◽  
Umeshkumar Nakum ◽  
Ravindra Patel
Keyword(s):  
Ulcerative Colitis ◽  
Ulcerative Colitis Patient ◽  
Chronic Inflammatory Demyelinating Polyneuropathy ◽  
Rare Complication ◽  
Gastrointestinal Symptoms ◽  
Demyelinating Polyneuropathy ◽  
High Dose ◽  
Initial Symptom ◽  
Colitis Patient ◽  
Rare Association

Chronic inammatory demyelinating polyneuropathy (CIDP) is a rare complication of Ulcerative colitis and it is uncertain whether it is associated with Ulcerative colitis itself or with its treatment. We describe a case of CIDP-like neuropathy as an initial symptom of Ulcerative colitis. A 17 years old male patient presented with symmetrical weakness of both lower extremities with paresthesia of both hand and feet. With impression of AIDP patient was treated with plasmapheresis and discharged on tapering steroid therapy. After 3 months again presented with bilateral weakness of all four limbs associated with diarrhea and fever. Patient was diagnosed with Ulcerative colitis ,so considering previous episode as 1st episode of CIDP and with rare association with Ulcerative colitis . Patient was treated with high dose of steroids and immunosuppressive therapy. Neurological and gastrointestinal symptoms remarkably improved after treatment.

scholarly journals Efficacy of 5-Aminosalicylic Acid Enemas in the Treatment of Distal Ulcerative Colitis

1990 ◽  
Vol 4 (7) ◽  
pp. 468-471 ◽  
Author(s):  
MG Robinson ◽  
DL Decktor
Keyword(s):  
Ulcerative Colitis ◽  
Disease Activity ◽  
Activity Index ◽  
Disease Activity Index ◽  
First Episode ◽  
High Dose ◽  
Aminosalicylic Acid ◽  
Open Label ◽  
Distal Ulcerative Colitis ◽  
The Mean

The efficacy of 4 g 5-aminosalicylic acid (5-ASA, mesalamine) enemas was assessed in 666 patients with distal ulcerative colitis. Patients were enrolled in an open-label compassionate use program. One 4 g 5-ASA enema was administered each night for a period of four weeks and the disease activity index was assessed at baseline and on days 14 and 28. On days 14 and 28, 78.0% and 88.1% of patients, respectively, demonstrated an improvement in disease activity index. The mean decline in disease activity index on day 14 was 40.7% (P=0.0001) and on day 28 it was 55.4% (P=0.0001). Efficacy was similar whether the disease was confined to or extended beyond 30 cm from the anus. There was no difference in efficacy in patients suffering their first episode of disease compared to patients suffering subsequent attacks. In conclusion, high dose 5-ASA enemas are a highly effective treatment for distal ulcerative colitis.

scholarly journals Molecular typing of bacteria of the genus Asaia in malaria vector Anopheles arabiensis Patton, 1905

2012 ◽  
Vol 44 (2) ◽  
pp. 7 ◽  
Author(s):  
S. Epis ◽  
M. Montagna ◽  
F. Comandatore ◽  
C. Damiani ◽  
A. Diabaté ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Internal Transcribed Spacer ◽  
Anopheles Arabiensis ◽  
Acetic Acid Bacterium ◽  
Sub Saharan Africa ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Sub Saharan ◽  
Dna Pcr

The acetic acid bacterium <em>Asaia</em> spp. was successfully detected in <em>Anopheles arabiensis</em> Patton, 1905, one of the major vector of human malaria in Sub-Saharan Africa. A collection of 45 <em>Asaia</em> isolates in cellfree media was established from 20 individuals collected from the field in Burkina Faso. 16S rRNA universal polymerase chain reaction (PCR) and specific qPCR, for the detection of <em>Asaia</em> spp. were performed in order to reveal the presence of different bacterial taxa associated with this insect. The isolates were typed by internal transcribed spacer-PCR, BOX-PCR, and randomly amplified polymorphic DNA-PCR, proved the presence of different <em>Asaia</em> in <em>A. arabiensis</em>.

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