scholarly journals Obturating Techniques in Pediatric Dentistry : Literature Review

2020 ◽  
Vol 3 (08) ◽  
Author(s):  
Raj Dalsania ◽  
Ayush Arora ◽  
Kunal Singla ◽  
Divya Vyas ◽  
Kriti Sareen ◽  
...  
Keyword(s):  
Pediatric Dentistry ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Primary Tooth ◽  
Filling Material ◽  
Pulp Tissue ◽  
Canal Systems ◽  
Pros And Cons ◽  
And Function ◽  
Micro Organisms

Primary teeth should be preserved until their normal exfoliation time so as to maintain arch length and function in order to provide proper guidance for the eruption of  permanent teeth , enhance esthetics and mastication, prevent aberrant tongue habits, aid in speech  and prevent the psychological effects associated with tooth loss. Pulpectomy consists of removing the pulp tissue associated with micro-organisms and debris from the canal and obturating with resorbable filling material. Success rate of endodontic therapy depends on many factors like  familiarity with the complexity of primary tooth canal systems, their formation and resorption pattern, obturating material as well as obturation technique used that is capable of densely filling the entire root canal system and providing a fluid tight seal from the apical segment of the canal to the cavosurface margin in order to prevent reinfection. This review article basically focuses on various obturating techniques used in deciduous teeth with their comparison, pros and cons.

Stem Cells Derived from Human Exfoliated Deciduous teeth [shed] in Neuronal Disorders: A Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Minu Anoop ◽  
Indrani Datta
Keyword(s):  
Stem Cells ◽  
Neurodegenerative Diseases ◽  
Dental Pulp ◽  
Therapeutic Potential ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Proliferative Potential ◽  
Pulp Tissue ◽  
Human Exfoliated Deciduous Teeth

: Most conventional treatments for neurodegenerative diseases fail due to their focus on neuroprotection rather than neurorestoration. Stem cell‐based therapies are becoming a potential treatment option for neurodegenerative diseases as they can home in, engraft, differentiate and produce factors for CNS recovery. Stem cells derived from human dental pulp tissue differ from other sources of mesenchymal stem cells due to their embryonic neural crest origin and neurotrophic property. These include both dental pulp stem cells [DPSCs] from dental pulp tissues of human permanent teeth and stem cells from human exfoliated deciduous teeth [SHED]. SHED offer many advantages over other types of MSCs such as good proliferative potential, minimal invasive procurement, neuronal differentiation and neurotrophic capacity, and negligible ethical concerns. The therapeutic potential of SHED is attributed to the paracrine action of extracellularly released secreted factors, specifically the secretome, of which exosomes is a key component. SHED and its conditioned media can be effective in neurodegeneration through multiple mechanisms, including cell replacement, paracrine effects, angiogenesis, synaptogenesis, immunomodulation, and apoptosis inhibition, and SHED exosomes offer an ideal refined bed-to-bench formulation in neurodegenerative disorders. However, in spite of these advantages, there are still some limitations of SHED exosome therapy, such as the effectiveness of long-term storage of SHED and their exosomes, the development of a robust GMP-grade manufacturing protocol, optimization of the route of administration, and evaluation of the efficacy and safety in humans. In this review, we have addressed the isolation, collection and properties of SHED along with its therapeutic potential on in vitro and in vivo neuronal disorder models as evident from the published literature.

scholarly journals EFFECT OF CHEWING LOAD DEFICIENCY ON STATE OF THE DENTAL SYSTEM IN CHILDREN

2021 ◽  
Vol 16 (4) ◽  
pp. 162-166
Author(s):  
Natalia Shtaeva ◽  
Antonina Anokhina ◽  
Said Ksembaev ◽  
Marina Yakovleva
Keyword(s):  
Root Resorption ◽  
Negative Impact ◽  
Pediatric Dentistry ◽  
Masticatory Muscles ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Jaw Bones ◽  
Abnormal Growth ◽  
Dental Pathology ◽  
Dental Diseases

Subject. A review of the literature is presented on the topical problem of pediatric dentistry — the development of major dental diseases in childhood due to the existing deficiency of chewing load. The aim of the study was to study the materials of publications on the influence of the deficit of chewing load in childhood on the development of dental pathology. Methodology. The effect of the deficit of the chewing load on the structures of the dentition in children, leading to hypofunction and a decrease in resistance to dental diseases, is described. Results. It was found that in children in the period of mixed bite, the occlusal load is distributed unevenly. One of the reasons for this is malocclusion. As a result, the functional state of the temporal and masticatory muscles changes. The simultaneous presence in the mouth of deciduous teeth that have lost their stability due to root resorption and permanent teeth, which are at various stages of eruption and root formation, leads to a significant decrease in chewing function, leading to poor oral hygiene, uneven training of the chewing muscles, abnormal growth of the jaw bones and the formation of dentoalveolar anomalies. Violation of the chewing function has a negative impact on the growth and development of the child, causing pathological adaptation of all structures to occlusion anomalies. Therefore, the question of the importance of active chewing, full load on the dentition is important for the prevention of periodontal disease, dental caries and the development of dentoalveolar anomalies. Conclusions. The results of the review indicate that there is a connection between the development of major dental diseases in children with a deficit in chewing load due to the widespread consumption of soft foods. In the available literature, there is an insufficient number of works devoted to the influence of the deficit of the chewing load on the development of major dental diseases. In our opinion, one of the ways to solve this problem can be the use of dentoalveolar training in childhood.

scholarly journals In vitro histomorphometric comparison of dental pulp tissue in different teeth

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8212
Author(s):  
Marytere Guerrero-Jiménez ◽  
Geovanny I. Nic-Can ◽  
Nelly Castro-Linares ◽  
Fernando Javier Aguilar-Ayala ◽  
Michel Canul-Chan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Density ◽  
Dental Pulp ◽  
Pearson Correlation ◽  
Third Molar ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Pulp Tissue ◽  
Valuable Source

Background Dental pulp (DP) represents an accessible and valuable source promising of stem cells for clinical application. However, there are some disadvantages associated with the isolation of dental pulp stem cells (DPSCs), which include the size and weight of the pulp tissue needed to yield sufficient cells for culturing in vitro. Therefore, the objective of this study was to compare in vitro histomorphometry of DP from permanent (premolars, third molar), supernumerary and deciduous teeth of patients between 5 and 25 years old with regards to weight, length, width and the cell density in the four regions of the DP in order to obtain quantitative parameters in a tissue that represents a valuable source of stem cells. Methods DPs were obtained from 10 central incisors deciduous, 20 permanent teeth (10 premolars, 10 third molars) and 10 supernumeraries (six mesiodents and four inferior premolar shapes). The pulps were carefully removed, and the entire tissue was weighed. The pulp length and the width were measured with a digital Vernier caliper. The cellular density analysis was performed according to the four regions of the DP (coronal, cervical, medial and apical) in histological slides using photography and the ImageJ® program for quantification. Results The Pearson correlation test revealed that DP weight among different types of teeth is correlated with age in male patients. A significant positive correlation was noted between length and width of the DP with age in both genders. The mean DP weight for supernumerary and third molar teeth was greater than deciduous and premolar teeth. Finally, the histological analysis showed that the coronal and apical portions of DP in supernumerary and premolar teeth have the highest cell density. Conclusions The DP of supernumerary teeth has quantitatively the best morphometric parameters and cell density comparable with the quality of DP obtained from deciduous teeth.

scholarly journals Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy

Biology ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 160 ◽  
Author(s):  
Shinichiro Yoshida ◽  
Atsushi Tomokiyo ◽  
Daigaku Hasegawa ◽  
Sayuri Hamano ◽  
Hideki Sugii ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Dental Pulp ◽  
High Growth ◽  
Dental Pulp Stem Cells ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Pulp Tissue ◽  
Differentiation Potential ◽  
Cell Based Therapy

Mesenchymal stem cells (MSCs) have the capacity for self-renewal and multilineage differentiation potential, and are considered a promising cell population for cell-based therapy and tissue regeneration. MSCs are isolated from various organs including dental pulp, which originates from cranial neural crest-derived ectomesenchyme. Recently, dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs) have been isolated from dental pulp tissue of adult permanent teeth and deciduous teeth, respectively. Because of their MSC-like characteristics such as high growth capacity, multipotency, expression of MSC-related markers, and immunomodulatory effects, they are suggested to be an important cell source for tissue regeneration. Here, we review the features of these cells, their potential to regenerate damaged tissues, and the recently acquired understanding of their potential for clinical application in regenerative medicine.

Pulp Therapy in Primary Teeth - Profile of teaching in Brazilian Dental Schools

2010 ◽  
Vol 35 (2) ◽  
pp. 191-195 ◽  
Author(s):  
Anieli Dossa Bergoli ◽  
Robert Eliot Primosch ◽  
Fernando Borba de Araujo ◽  
Thiago Machado Ardenghi ◽  
Luciano Casagrande
Keyword(s):  
Calcium Hydroxide ◽  
Multiple Choice ◽  
Pediatric Dentistry ◽  
Primary Tooth ◽  
Tooth Pulp ◽  
Filling Material ◽  
Multiple Choice Questions ◽  
Pulp Therapy ◽  
Clinical Scenarios ◽  
Dental Schools

This study investigates the profile of teaching primary tooth pulp therapy practiced by Brazilian dental schools. A multiple-choice questionnaire was sent by e-mail to 191 dental schools in Brazil, addressed to the pediatric dentistry Chairperson. The two-part survey consisting of multiple-choice questions regarding specific materials and techniques on pulp therapies, moreover, hypothetical clinical scenarios were presented so that the respondents could guide the treatment approach. The questionnaires were returned by 46.5% of the dental schools. Ninety-five percent of surveyed schools teach IPT for the treatment of deep carious lesions in dentin and indicate the calcium hydroxide as capping material (59,3%). The direct pulp capping is taught by 68.7% of schools and calcium hydroxide (97%) was the capping material most indicated. Pulpotomy is taught in 98.7% of schools and formocresol (1:5 dilution) was the medicament of choice(50%). All schools taught pulpectomy and Iodoform paste was the filling material preferred (55%). The results showed a lack of consensus in certain modalities and techniques for primary tooth pulp therapy taught by Brazilian dental schools.

ROTARY ENDODONTICS IN PEDIATRIC DENTISTRY- A COMPREHENSIVE REVIEW

2021 ◽  
pp. 9-11
Author(s):  
Thakur Anita ◽  
Thakur Seema
Keyword(s):  
Primary Teeth ◽  
Pediatric Dentistry ◽  
Primary Dentition ◽  
Permanent Teeth ◽  
Primary Tooth ◽  
Comprehensive Review ◽  
Pulp Therapy ◽  
Functional Part

The goal of pulp therapy in the primary dentition is to retain the primary tooth as a fully functional part of the dentition, allowing at the same time for mastication, phonation, swallowing, and the preservation of the space required for the eruption of the permanent 1,2 tooth . The premature loss of primary teeth may cause changes in the chronology and sequence of eruption permanent teeth. Maintenance of primary teeth until physiological exfoliation prevents deleterious 3 habits in children .

scholarly journals A cytotoxic evaluation of 7 th generation dentin bonding agent on human pulp cells

2016 ◽  
Vol 8 (2) ◽  
pp. 63
Author(s):  
Salwani A. Ghani ◽  
Sam’an M. Masudi ◽  
Norshamsuria Omar
Keyword(s):  
Bonding Agent ◽  
Deciduous Teeth ◽  
Filling Material ◽  
Pulp Tissue ◽  
Bonding Agents ◽  
Dentin Bonding ◽  
Dentin Bonding Agents ◽  
Dentin Bonding Agent ◽  
Pulp Cells

Dentin bonding agents are resin based materials that used in clinical dentistry inorder to prevent leakage and promote adherent of filling material to the enamel anddentin. However, the polymerization of dentin bonding agents will release residualmonomer that may interact with pulp tissue. This in vitro study is aimed to evaluatethe cytotoxicity of new 7thgeneration dentin bonding agents (G Bond) on stem cellsfrom human exfoliated deciduous teeth (SHEDs). The proliferated human pulp cellswere incubated at 37 C for 48 hours. Under aseptic conditions, extracted testspecimen were plated in 96 well of tissue tray. SHEDs were placed on each well withdifferent concentration of dentin bonding agents, and then incubated at 37 C for 72hours exposure. The cytotoxic effect was recorded by using MTT assay method.Statistical analysis showed that higher concentration of dentin bonding agents (IC=0.035 mg/ml)exerts higher toxic effect to the human pulp cells (SHEDs). This studyconcluded that pulpal reaction to dentin bonding agent may depend on number ofapplication procedure.

scholarly journals Total Impaction of Deciduous Maxillary Molars: Two Case Reports

2007 ◽  
Vol 8 (6) ◽  
pp. 64-71 ◽  
Author(s):  
Kaan Gündüz ◽  
Mehtap Muğlali ◽  
Samet Inal
Keyword(s):  
Maxillary Sinus ◽  
Case Reports ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Primary Tooth ◽  
Inferior Wall ◽  
Maxillary Molars ◽  
Tooth Impaction ◽  
Deciduous Molar ◽  
Deciduous Molars

Abstract Aim The purpose of this report is to present two cases of totally impacted maxillary deciduous molars, considered a rarity in dental practice. Background Primary tooth impaction is quite rare during the development of primary dentition. Various factors contribute to the impaction of a deciduous tooth, including anklyosis, congenitally missing permanent teeth, defects in the periodontal membrane, trauma, injury of the periodontal ligament, precocious eruption of the first permanent molar, defective eruptive force, or a combination of these factors. Reports Case #1 An 18-year-old male presented with a complaint of spontaneous repetitious pain in the maxillary right premolar region. The maxillary right second premolar was clinically absent. Panoramic and periapical radiographs revealed an impacted second premolar close to the inferior wall of the maxillary sinus and an impacted deciduous molar deeply embedded in bone within the maxillary sinus. Case #2 A 14-year-old girl presented with a complaint of crowding of the maxillary teeth. The maxillary right second premolar and the maxillary permanent canines were clinically absent. A panoramic radiograph revealed an impacted maxillary right second premolar and an impacted deciduous molar embedded within bone close to the inferior wall of the maxillary sinus. Summary The total impactation of deciduous teeth is a rare condition, and few cases have been reported in the literature. The condition generally affects the mandibular second deciduous molar and the maxillary first deciduous least often. In this paper, two cases of totally impacted maxillary deciduous molars are reported. Citation Gündüz K, Muğlali M, Inal S. Total Impaction of Deciduous Maxillary Molars: Two Case Reports. J Contemp Dent Pract 2007 September; (8)6:064-071.

Regenerative potential of stem cells derived from human exfoliated deciduous (SHED) teeth during engineering of human body tissues

2020 ◽  
Vol 16 ◽  
Author(s):  
Shivkanya Fuloria ◽  
Ajay Jain ◽  
Sameep Singh ◽  
Iswar Hazarika ◽  
Samson Salile ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Body ◽  
In Vivo Studies ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Pulp Tissue ◽  
Human Teeth ◽  
Body Tissues

Abstract:: Current decade witnesses the regenerative potential of stem cells (SCs) based life-saving therapies for the treatment of various disease and conditions. Human teeth act as reservoir for SCs that exist with high abundance in baby, wisdom, and permanent teeth. The collection of stem cells from human exfoliated deciduous teeth (SHED) is considered as a simple process as it offers the convenience of little or no pain. In comparison to the SCs from dental or bone marrow or other tissues, the SHED offers the benefit of higher cellular differentiation and proliferation. Massive in vitro and in vivo studies reveal the regenerative potential of SHED in the engineering of dental pulp tissue, neuronal tissue, root, bio root, cardiovascular tissues, lymphatic tissues, renal tissues, dermal tissues, hepatic tissues, and bone tissues. Current review describes the methods of collection/isolation/storage, various biomarkers, and types of SHED. This review highlights the regenerative potential of SHED in the engineering of different tissues of human body. As per the available research evidences present study supports that SHED may differentiate into the endothelial cells, neurons, odontoblasts, pancreatic

scholarly journals Comparative evaluation of osteogenic differentiation potential of stem cells derived from dental pulp and exfoliated deciduous teeth cultured over granular hydroxyapatite based scaffold

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Manal Nabil Hagar ◽  
Farinawati Yazid ◽  
Nur Atmaliya Luchman ◽  
Shahrul Hisham Zainal Ariffin ◽  
Rohaya Megat Abdul Wahab
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
3D Culture ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Osteogenic Potential ◽  
Control Group ◽  
Rt Pcr ◽  
Differentiation Potential

Abstract Background Mesenchymal stem cells isolated from the dental pulp of primary and permanent teeth can be differentiated into different cell types including osteoblasts. This study was conducted to compare the morphology and osteogenic potential of stem cells from exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSC) in granular hydroxyapatite scaffold (gHA). Preosteoblast cells (MC3T3-E1) were used as a control group. Methodology The expression of stemness markers for DPSC and SHED was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR). Alkaline phosphatase assay was used to compare the osteoblastic differentiation of these cells (2D culture). Then, cells were seeded on the scaffold and incubated for 21 days. Morphology assessment using field emission scanning electron microscopy (FESEM) was done while osteogenic differentiation was detected using ALP assay (3D culture). Results The morphology of cells was mononucleated, fibroblast-like shaped cells with extended cytoplasmic projection. In RT-PCR study, DPSC and SHED expressed GAPDH, CD73, CD105, and CD146 while negatively expressed CD11b, CD34 and CD45. FESEM results showed that by day 21, dental stem cells have a round like morphology which is the morphology of osteoblast as compared to day 7. The osteogenic potential using ALP assay was significantly increased (p < 0.01) in SHED as compared to DPSC and MC3T3-E1 in 2D and 3D cultures. Conclusion gHA scaffold is an optimal scaffold as it induced osteogenesis in vitro. Besides, SHED had the highest osteogenic potential making them a preferred candidate for tissue engineering in comparison with DPSC.

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