scholarly journals Molecular detection and phylogenetic analysis of Mycoplasma Spp. isolated from awassi sheep in Al-Muthana Province, Iraq

2020 ◽  
Vol 23 (1) ◽  
pp. 21-28
Author(s):  
Q. H. KSHASH ◽  
L. A. AL-RAOW
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Detection ◽  
Molecular Study ◽  
Mycoplasma Species ◽  
Rrna Gene ◽  
High Similarity ◽  
Chain Reaction ◽  
Awassi Sheep ◽  
Polymerase Chain ◽  
Mycoplasma Spp

This study was carried out for molecular detection and phylogenetic analysis of Mycoplasma spp. in Awassi sheep in Al-Muthana province, Iraq. A total of 270 milk samples and swabs were collected from infected sheep. A polymerase chain reaction (PCR) technique was performed to detect a specific 16S rRNA gene of Mycoplasma spp. in these samples. Forty-four positive samples (16.2%) were identified, from which eight samples were selected for partial-gene sequencing. Then, alignment, com-parison with referencing isolates in GenBank, and phylogenetic tree were performed using mean (UPGMA tree) in a MEGA software. The analyses revealed high homology between the current Iraqi isolates and American and Sweden Mycoplasma strains. The present molecular study showed that the studied Iraqi Awassi sheep were infected with Mycoplasma spp. with higher detection percentage from ocular swabs than from other types of samples. The phylogenic analysis registered eight Iraqi isolates with accession numbers in the GenBank with high similarity to five referencing Mycoplasma species.

scholarly journals Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory

2016 ◽  
Vol 31 (1) ◽  
pp. 29-31
Author(s):  
Marieke Brauer ◽  
Marianne Wolfaardt ◽  
Lynne M. Webber ◽  
Maureen B. Taylor
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
Cerebrospinal Fluid ◽  
Aseptic Meningitis ◽  
Reverse Transcription ◽  
Molecular Detection ◽  
Mumps Virus ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis.

scholarly journals Hematological changes associated with hemoplasma infection in cats in Rio de Janeiro, Brazil

2016 ◽  
Vol 25 (4) ◽  
pp. 441-449 ◽  
Author(s):  
Juliana Macedo Raimundo ◽  
Andresa Guimarães ◽  
Raisa Braul Rodrigues ◽  
Camila Flávia Magalhães Botelho ◽  
Maristela Peckel Peixoto ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Rio De Janeiro ◽  
Conventional Polymerase Chain Reaction ◽  
Metropolitan Region ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Mycoplasma Spp

Abstract This study aimed to detect Mycoplasma spp. in naturally infected cats from Rio de Janeiro and to evaluate hematological abnormalities and factors associated with this infection. Out of the 197 cats sampled, 11.2% presented structures compatible with hemoplasma organisms on blood smears. In contrast, 22.8% were positive for Mycoplasma spp. by means of 16S rRNA gene real-time polymerase chain reaction, which reflects the weak concordance between techniques. The infection rates, by means of 16S rRNA gene conventional polymerase chain reaction, was 4.6%, 4.6% and 11.7% for Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma turicensis’ (CMt) and ‘Candidatus Mycoplasma haemominutum’ (CMhm), respectively. Mhf and CMhm infections are more frequent in the summer (p>0.05). Presence of anemia (p < 0.02), lymphocytosis (p < 0.03), thrombocytopenia (p < 0.04) and activated monocytes (p < 0.04) was associated with Mhf infection. No hematological abnormality was associated with CMt or CMhm infection. Male cats were more prone to be infected by Mhf or CMhm (p < 0.01). Adult cats had more chance to be infected by CMhm. Three hemoplasma species occur in the metropolitan region of Rio de Janeiro and Mhf seems to be the most pathogenic of them. Anemia is the most important hematological abnormality.

scholarly journals Molecular detection of Campylobacter species from human and cattle faecal samples in Kilosa district, Tanzania

2020 ◽  
Author(s):  
Noel Gahamanyi ◽  
Leonard E.G. Mboera ◽  
Mecky I. Matee ◽  
Dieudonné Mutangana ◽  
Raghavendra G. Amachawadi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Faecal Samples ◽  
Rrna Sequencing ◽  
Polymerase Chain ◽  
Campylobacter Species ◽  
The 16S Rrna Gene

Abstract Background: A growing number of Campylobacter species other than C. jejuni and C. coli have been considered as emerging human and animal pathogens. However, the contribution of these species to human gastroenteritis is poorly documented. This study aimed at detecting Campylobacter species from human and cattle faecal samples in Kilosa district, Tanzania using Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene, and Sanger sequencing . Methods: A total number of 100 faecal samples (70 from human and 30 from cattle) were collected from diarrheic and non-diarrheic patients and healthy cattle in Kilosa district, Tanzania from July to October 2019. Species identification was conducted by PCR and 16S rRNA sequencing. The phylogenetic analysis was carried out by comparison of the 16S rRNA gene sequences to reference strains by the Neighbor-Joining method in MEGA X. Results: Campylobacter species detection rate by PCR was 65.7% (46/70) and 20% (6/30) in humans and cattle, respectively. There were five human diarrheic cases, four showed Campylobacter presence and two were from children ≤15 years of age. In humans, the 16S rRNA sequencing revealed that C. concisus was the most predominant species occurring at a frequency of 37.8% (14/37), followed by uncultured Campylobacter spp. 24.3% (9/37) and C. hominis 21.6% (8/37). The least represented species were C. jejuni and C. lanienae all occurring at 2.7% (1/37). In cattle, five (100%) sequenced PCR products matched with C. lanienae . Phylogenetic analysis revealed that Campylobacter 16S rRNA sequences were closely related to C. concisus , uncultured Campylobacter sp., C. hominis , and C. gracilis . Conclusion: The non- C. jejuni / C. coli species are present in human and cattle faecal samples and their true occurrence is probably under-reported due to shortcomings of conventional techniques used in most diagnostic microbiology laboratories. Based on our findings, we recommend that molecular techniques be adopted for direct detection of Campylobacter species during routine laboratory screening and surveillance studies. Keywords: Campylobacter , molecular diagnostics, polymerase chain reaction, sequencing, gastroenteritis, Tanzania

scholarly journals Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil

2021 ◽  
Vol 42 (6) ◽  
pp. 3527-3534
Author(s):  
Mayara Marques Pereira Fernandes ◽  
◽  
Marcelo Renan Serrate Rodrigues ◽  
Jessica Damiana Marinho Valente ◽  
Marcelli Pascoal Nogueira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Detection ◽  
Clinical Signs ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Babesia Vogeli ◽  
Physical Examinations ◽  
Northern Brazil ◽  
Polymerase Chain

This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral blood), and polymerase chain reaction (PCR) for piroplasm based on the 18S rRNA gene, were performed. One dog (1/47, 2.1%; CI 95%: 0.1-11.3%) tested positive to Babesia vogeli in the polymerase chain reaction (PCR) assay for piroplasms and the resulting sequence showed 100% identity with Babesia vogeli isolates deposited in GenBank®. Co-infection with Ehrlichia spp. was also observed by direct examination (via blood smear). The clinical and hematological alterations observed in the positive animal were anorexia, dehydration, white mucous membranes, anemia and thrombocytopenia.

scholarly journals Detection and genetic characterization of "Candidatus Mycoplasma haemomacaque" infection among long-tailed macaques (Macaca fascicularis) in Thailand using broad-range nested polymerase chain reaction assay

2021 ◽  
Vol 14 (4) ◽  
pp. 943-948
Author(s):  
Wanat Sricharern ◽  
Supakarn Kaewchot ◽  
Sarawan Kaewmongkol ◽  
Natnaree Inthong ◽  
Thitichai Jarudecha ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Genetic Characterization ◽  
Rrna Gene ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Blood Samples ◽  
Polymerase Chain ◽  
The 16S Rrna Gene

Background and Aim: Hemoplasmas are defined as small, epicellular parasitic bacteria that can infect the red blood cells of several mammalian species. Diseases caused by these bacteria range from asymptomatic infections to acute hemolytic anemia. However, data on hemoplasmas in non-human primates in Thailand remain to be limited. Therefore, this study aims to determine the occurrence and genetic diversity of hemoplasmas among long-tailed macaques in Thailand. Materials and Methods: Blood samples were collected from 339 long-tailed macaques in three provinces of Thailand. DNA was then extracted from the blood samples and tested for hemoplasma using broad-range nested polymerase chain reaction (PCR) based on the 16S rRNA gene. PCR-positive samples were sequenced, and phylogenetic analysis for species identification was conducted. Results: In total, 38 (11.2%) out of the 339 samples were found to be positive for hemoplasmas, based on the broad-range nested PCR assay of the 16S rRNA gene. The 16S rRNA sequences of Mycoplasma spp. were highly similar (98-99% identity) to "Candidatus Mycoplasma haemomacaque." Furthermore, phylogenetic analysis using maximum likelihood demonstrated that the sequences were located in the same cluster of "Ca. M. haemomacaque." Conclusion: The detection of hemoplasmas among long-tailed macaques in Thailand is reported. Genetic characterization confirmed that these hemoplasmas are closely related to "Ca. M. haemomacaque." These results indicate that long-tailed macaques in several locations in Thailand may be infected and serve as reservoirs for this parasite.

scholarly journals Phytoplasma occurrence in apple trees in the Czech Republic

2011 ◽  
Vol 39 (No. 1) ◽  
pp. 7-12 ◽  
Author(s):  
R. Fialová ◽  
M. Navrátil ◽  
P. Válová
Keyword(s):  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
Polymorphism Analysis ◽  
Apple Proliferation ◽  
Chain Reaction ◽  
Apple Trees ◽  
The Czech Republic ◽  
High Incidence ◽  
Phytoplasma Infection ◽  
Polymerase Chain

The presence of phytoplasmas in apple trees with proliferation symptoms, rubbery wood symptoms and no symp&shy;toms was determined by using polymerase chain reaction assays with primers amplifying phytoplasma 16S rRNA gene. Phytoplasmas were detected in all trees with proliferation symptoms. Positive tests for phytoplasma in the group of trees with rubbery wood symptoms and of those without symptoms revealed a relatively high incidence of latent phytoplasma infection. Using restriction fragment length polymorphism analysis, phytoplasma of the same identity &ndash; apple proliferation phytoplasma (subgroup 16SrX-A) &ndash; was recorded in all positively tested trees. &nbsp;

Polymerase chain reaction technique for molecular detection of HPV16 infections among women with cervical cancer in Dhi-Qar Province

2021 ◽  
Author(s):  
Abduladheem Turki Jalil ◽  
Ali Hussein Demin Al-Khafaji ◽  
Aleksandr Karevskiy ◽  
Saja Hussain Dilfy ◽  
Zaman K. Hanan
Keyword(s):  
Cervical Cancer ◽  
Polymerase Chain Reaction ◽  
Molecular Detection ◽  
Polymerase Chain Reaction Technique ◽  
Chain Reaction ◽  
Polymerase Chain
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