CHARACTERISTICS OF NORSODYNE AS PLASTINATION AGENT

Background: Norsodyne is a new material for conservation and durable preservation of biological objects. The form we use is H 13212 TAE (Polynt Composites USA, Inc., Bergamo, Italy). This is an unsaturated polyester resin in styrene that is of low styrene emission. Features: resin has a specific weight 1.10 g/cm2 at 20°C. Using test method MT-CUT23V the manufacturer has established that Brookfield viscosity at 23°C is 450-650 mPa.s. The jelly starts in 14-18 minutes and reaches its peak in 27-37 minutes at 23°C too. Acording to the test results of ISO 527 (2012) tensile strength of Norsodyne is 57 MPa and flexural strength is 98 MPa (ISO 178 (2011). Conclusion: This characteristic of Norsodyne indicates that the material can be used for the preservation and long-term storage of anatomical preparations for the needs of anatomy training. However, the Norsodyne needs special storage conditions. The material should be stored in a dark place. According to the manufacturer shelf life at 23°C in the dark is six months then spontaneous rubbing begins. The manufacturer's recommendations are: Store in the shade, out of direct sunlight. Keep storage temperature below 25°C. Unseal container just before use. Shelflife will be reduced reaching higher temperature.

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Storage Temperature, Controlled Atmosphere, and 1-Methylcyclopropene Effects on α-Farnesene, Conjugated Trienols, and Peroxidation in Relation with Superficial Scald, Pithy Brown Core, and Fruit Quality of ‘d’Anjou’ Pears during Long-term Storage

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.141.2.177 ◽

2016 ◽

Vol 141 (2) ◽

pp. 177-185 ◽

Cited By ~ 10

Author(s):

Yan Wang

Keyword(s):

Storage Temperature ◽

Storage Conditions ◽

Controlled Atmosphere ◽

Metabolic Balance ◽

Superficial Scald ◽

Long Term Storage ◽

Temperature Controlled ◽

Term Storage

Alternatives to ethoxyquin (Etq) are needed for controlling superficial scald of ‘Anjou’ european pears (Pyrus communis) during long-term storage. The current commercial standard storage conditions [Etq + −1 °C + controlled atmosphere (CA) with 1.5 kPa O2] reduced scald occurrence compared with control fruit (−1 °C + CA) during 6–8 months storage. At 1 °C in air, 1-methylcyclopropene (1-MCP) fumigation at 0.15 µL·L−1 at harvest was more efficient on reducing scald than Etq but did not prevent scald during 6–8 months storage. The 1-MCP-treated fruit at 1 °C in air developed their ripening capacity at 20 °C following 6–8 months storage but had deceased shipping ability (softening and yellowing of fruit). Although Etq inhibition of scald was associated with the inhibition of α-farnesene oxidation to conjugated trienols (CTols); 1-MCP reduced α-farnesene synthesis and thereby the availability of substrate to oxidize to CTols. CA storage at 1.5 kPa O2 totally prevented scald and retarded the loss of shipping ability without affecting the ripening capacity of 1-MCP-treated fruit at 1 °C through further decreases in the syntheses of ethylene, α-farnesene and CTols during 6–8 months storage. In addition, 1-MCP prevented a CA-induced disorder, pithy brown core (PBC), in ‘Anjou’ pears possibly through enhancing an oxidative/reductive metabolic balance during extended storage. In conclusion, the combinations of 1 °C + 1-MCP + CA is a potential commercial alternative to Etq for scald control while allowing the 1-MCP-treated ‘Anjou’ pears to recover ripening capacity during the shelf life period after 6–8 months storage.

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Efficacy of the Antigenicity-Retaining Ability of Fixative Solutions for Liquid-Based Cytology: Immunocytochemistry of Long-Term Storage

Acta Cytologica ◽

10.1159/000518452 ◽

2021 ◽

pp. 1-12

Author(s):

Hiaki Sato ◽

Yoshiaki Norimatsu ◽

Satoshi Irino ◽

Takeshi Nishikawa

Keyword(s):

Cell Membrane ◽

Room Temperature ◽

Storage Temperature ◽

Storage Period ◽

Storage Conditions ◽

Immunocytochemical Staining ◽

Long Term Storage ◽

Liquid Based Cytology ◽

Term Storage

Introduction/Objective: Liquid-based cytology (LBC) is advantageous as multiple stained specimens can be prepared and used for additional assays such as immunocytochemical and molecular-pathological investigations. Two types of preservative-fixative solutions (fixatives) are used for nongynecologic specimens used in the BD SurePath-LBC (SP-LBC) method, and their components vary. However, few studies have evaluated the differences in antigen-retaining ability between these fixatives. Therefore, we investigated and compared the antigen-retaining ability of the fixatives in immunocytochemical staining (ICC) under long-term storage conditions. Materials and Methods: Sediments of cultured RAJI cells (derived from Burkitt’s lymphoma) were added to each fixative (red and blue) and stored at room temperature for a specified period (1 h; 1 week; and 1, 3, and 6 months). The specimens were then prepared using the SP-LBC method and subjected to ICC. Positivity rate was calculated using the specimens fixed at room temperature for 1 h as a control. Antibodies against Ki67 expressed in the nucleus and against CD20 and leukocyte common antigen (LCA) expressed on the cell membrane were used. Results: For CD20 and LCA, the positivity rate increased with time in the red fixative compared with that in the control. In the blue fixative, the positivity rate was highest at 1 h and was maintained at a high level throughout the storage period. In contrast, the Ki67 positivity rate was highest at 1 h in both red and blue fixatives and markedly decreased with time. Therefore, although refrigerated (8°C) storage was used, no improvement was noted. Conclusions: Long-term storage is possible for cell membrane antigens at room temperature; however, it is unsuitable for intranuclear antigens. Therefore, we conclude that suitable fixative type and storage temperature differ based on antigen location. Further investigation is warranted.

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Kinetic Analysis of Stabilizing C-Phycocyanin in the Spirulina platensis Extracts from Ultrasonic Process Associated with Effects of Light and Temperature

Applied Sciences ◽

10.3390/app8091662 ◽

2018 ◽

Vol 8 (9) ◽

pp. 1662 ◽

Cited By ~ 2

Author(s):

Woon Yong Choi ◽

Hyeon Yong Lee

Keyword(s):

Storage Temperature ◽

Extraction Process ◽

Storage Conditions ◽

Second Order ◽

Light Illumination ◽

First Order ◽

Long Term Storage ◽

Ultrasonic Process ◽

Term Storage

A kinetic model was developed to reflect the stability of C-Phycocyanin (C-PC) from ultrasonic extraction process under different storage conditions. The decrease of C-PC contents was most accelerated at a high temperature of 40 °C along with light illumination, resulting in ca. 60% drop of an initial concentration for two months of storage. However, ca. 93% of the initial contents remained at 4 °C without light, which would be a most favorable condition for long-term storage. It was first shown that the decrease of the residual concentrations followed second-order kinetics under light illumination. However, without light, the decrease of the C-PC contents showed first order reaction kinetics, which implies initial C-PC concentrations are important. On the contrary, initial storage temperature seemed to be more influential under light illumination. It was also first revealed that the rate of degrading the C-PC was faster with light than without light even at the same temperature, having 0.0108 (1/h) and 0.0138 (1/h) of rate constants of first order (no light) and second order kinetics (with light) at 40 °C, respectively. Moreover, the cleavage of C-PC was also found to follow the Arrhenius relationship. Therefore, this work could provide desirable storage conditions of C-PC from non-thermal ultrasonic process for long-term storage.

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Dependence of fat acidity value on wheat grain storage conditions

BIO Web of Conferences ◽

10.1051/bioconf/20201700107 ◽

2020 ◽

Vol 17 ◽

pp. 00107

Author(s):

I. A. Kechkin ◽

V. A. Ermolaev ◽

M. V. Ivanov ◽

A. I. Romanenko ◽

E. A. Gurkovskaya

Keyword(s):

Storage Temperature ◽

Storage Period ◽

Acid Value ◽

Storage Conditions ◽

Air Humidity ◽

Wheat Grain ◽

Storage Duration ◽

Long Term Storage ◽

Relative Air Humidity

The article presents the dependence of the fat acidity value (FAV) on the values of humidity and temperature, the relationship between the storage duration for wheat grain and FAV. To establish the expiration date of wheat grain during long-term storage, the author of the article considered the fat acid value (FAV) in mg of KOH. Storage temperature and relative air humidity in a desiccator affect the change (growth) of fat acidity value. The greatest changes occurred at 6th, 7th and 8th months of storage at a relative air humidity of more than 65 % and temperatures above 20 °C. At a storage temperature of 10 °C, in all cases the growth of FAV remained insignificant and was within the limits of determination accuracy. It is noted that when the relative humidity was below 60 %, while the temperature was the same as in the previous case, the FAV of wheat grain was practically unchanged through the 6-month storage period.

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The Effect of Storage Temperature and Physical State on the Performance of Antisera in Radioimmunoassay after Long-Term Storage

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456328802500113 ◽

1988 ◽

Vol 25 (1) ◽

pp. 89-95

Author(s):

B A Middleton ◽

L M Morgan ◽

G W Aherne ◽

V Marks

Keyword(s):

Room Temperature ◽

Storage Temperature ◽

Storage Conditions ◽

Liquid Form ◽

Long Term Storage ◽

Freeze Dried ◽

Control Serum ◽

Term Storage ◽

Temperature Storage

The performance in radioimmunoassay of four antisera after storage at temperatures ranging from −40°C to room temperature, in three physical states (frozen, liquid or freeze dried) was investigated over a 3-year period. No deterioration in antiserum performance in terms of precision and accuracy of quality control serum measurement or recovery of ligand was apparent under any of the storage conditions studied. Some lowering of titre became apparent in two of the antisera over the study period. Deterioration was most marked when antiserum was stored lyophilised at room temperature. Storage of antiserum frozen confers no advantage over storage at 4°C provided precautions are taken to minimise bacterial contamination when storing antiserum in liquid form.

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The effect of storage temperature and duration on northern red oak acorn viability and vigour

The Forestry Chronicle ◽

10.5558/tfc2013-139 ◽

2013 ◽

Vol 89 (06) ◽

pp. 769-776 ◽

Cited By ~ 2

Author(s):

Thomas L. Noland ◽

Andrée E. Morneault ◽

Daniel C. Dey ◽

Dave Deugo

Keyword(s):

Storage Temperature ◽

Storage Conditions ◽

Red Oak ◽

Northern Red Oak ◽

Root Regrowth ◽

Long Term Storage ◽

High Germination ◽

Percent Germination ◽

Term Storage

Three separate collections of Ontario sources of northern red oak (Quercus rubra L.) acorns were made to determine the effects of long-term cold storage at +2°C, −1°C, and −2°C on their viability and vigour. We measured acorn moisture content, percent germination during storage, speed of germination and total germination values, root regrowth of seeds that germinated in storage, incidence of fungal contamination, and condition of ungerminated acorns. Viability and vigour peaked six to 12 months after acorns were placed in storage, but decreased with continued storage. After 18 months in storage, ≥60% of the acorns germinated in four of the five seedlots tested and, after 30 months in storage, ≥53% of the acorns germinated in three of the five seedlots tested. Acorn viability was only minimally affected by storage temperature; however, since temperatures above −2°C allowed acorns to germinate during storage, the preferred long-term storage temperature was −2°C. Our results suggest that, assuming proper storage conditions, most red oak seedlots will maintain relatively high germination levels when stored at −2°C for 18 months. Seedlots with particularly high initial germination and vigour may be successfully stored for up to 30 months.

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9975 Shipping Package: Performance of Alternate Materials for Long-Term Storage Application

ASME 2010 Pressure Vessels and Piping Conference: Volume 7 ◽

10.1115/pvp2010-25279 ◽

2010 ◽

Author(s):

T. E. Skidmore ◽

W. L. Daugherty ◽

E. N. Hoffman

Keyword(s):

Storage Conditions ◽

Test Results ◽

Savannah River ◽

Long Term Storage ◽

Additional Testing ◽

Long Term Performance ◽

Term Performance ◽

River Site ◽

Term Storage

The Model 9975 shipping package specifies the materials of construction for its various components. With the loss of availability of material for two components (cane fiberboard overpack and Viton® GLT O-rings), alternate materials of construction were identified and approved for use for transport (softwood fiberboard and Viton® GLT-S O-rings). The shipping packages are part of a long-term storage configuration at the Savannah River Site (SRS). Therefore, additional testing is in progress to verify satisfactory long-term performance of the alternate materials under storage conditions. The test results to date can be compared to results on the original materials of construction to draw preliminary conclusions on the performance of the replacement materials.

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Investigation of stability of intrauterine aerosol preparation “Yodozol”

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet9206 ◽

2018 ◽

Vol 20 (92) ◽

pp. 29-33

Author(s):

R. M. Sachuk ◽

O. A. Katsaraba

Keyword(s):

Shelf Life ◽

Medicinal Product ◽

Storage Temperature ◽

Storage Conditions ◽

Analgesic Effects ◽

Aerosol Preparation ◽

Long Term Storage ◽

The Stability ◽

Term Storage

For standardization, quality control, study of stability and establishment of storage conditions and terms of use, complex preclinical trials of the new development of PE “Biopharm” and the Experimental Station of Epizootology IVM NAAN – aerosol preparation “Yodozol” have been carried out. The methods of evaluation of a medicinal product applied to aerosols are used, which include: determination of changes in appearance, inspection of packaging for leakproofness, measurement of the percentage of contents of the package, the establishment of qualitative and quantitative indicators of active substances, and also the study of microbiological purity of the product. “Yodozol” is a light yellow liquid, 1 ml of which contains 5 mg of iodine and 10 mg of potassium iodide. The drug is used for the prevention and treatment of postnatal intrauterine infections in cows, pigs, sheep and goats (endometritis, pyrometers, cervicitis, vaginitis, delayed digestion caused by microorganisms sensitive to iodine), after obstetrics aid, cesarean section and postpartum sanitation of the uterus. The drug has antimicrobial, anti-inflammatory and analgesic effects, improves the proliferative processes of the genital organs, reduces the time for recovery of animals. The drug is used according to the guidelines, after its production livestock is used without restrictions. The shelf-life, which is the result of the test of the dasg according to the «stability» indicator, has been determined, which was performed under long-term storage in a place protected from light at a temperature range from + 5 ± 2 °С to + 25 ± 2 °С. The studies conducted after 6, 12, 24 and 30 months showed complete compliance of the quality indices with the declared standards when stored for 24 months in the temperature corridor from + 5 °C to + 20 °C. With an increase in storage temperature to + 25 °C or more, a slight quantitative decrease in the concentration of antimicrobial components occurred. In addition, with long-term storage of drugs, release of the contents from the cylinder became uneven and foam acquired a shade less than the saturation rate, increased microbiological contamination. Thus, according to the results of the study, the established shelf life of the preparation is 2 years at the recommended storage temperature from +5 to +20 °С. All studies conducted on the stability of the aerosol intrauterine drug “Yodozol” were included in the registration materials of the medicinal product.

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