scholarly journals Reference intervals for haematological parameters in the Lusitano horse breed

2018 ◽  
Vol 66 (4) ◽  
pp. 530-541 ◽  
Author(s):  
Ana Cristina Silvestre-Ferreira ◽  
Mário Cotovio ◽  
Mário Maia ◽  
Felisbina Queiroga ◽  
M João Pires ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Age Groups ◽  
Haemoglobin Concentration ◽  
Reference Intervals ◽  
Haematological Parameters ◽  
Distribution Width ◽  
Mean Values ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin

The Lusitano horse is an autochthonous Portuguese breed with a growing worldwide expansion. Our objective was to establish reference intervals for haematological parameters using the haematological cell counter LaserCyte (IDEXX). For this purpose, blood samples from 100 healthy adult horses (13 females and 87 males, ranging from 3 to 25 years of age) were analysed. The reference intervals were estimated following the ASVCP guidelines with the Reference Value Advisor software. The obtained reference intervals were 6.4–10.1 × 1012/L for red blood cells, 30.6–45.1% for haematocrit, 11.6–17.1 g/dL for haemoglobin, 42.8–53.2 fL for mean corpuscular volume (MCV), 15.5–20.8 pg for mean corpuscular haemoglobin (MCH), 33.7–39.4 g/dL for mean corpuscular haemoglobin concentration, 17.8–20.3% for red cell distribution width (RDW), 4.5–10.1 × 109/L for white blood cells, 2.2–6.0 × 109/L for neutrophils, 0.9–4.9 × 109/L for lymphocytes, 0.2–0.5 × 109/L for monocytes, 0.1–0.6 × 109/L for eosinophils, 0.0–0.1 × 109/L for basophils, 78.5–172.2 K/mL for platelets, 4.3–9.4 fL for mean platelet volume, 18.8–24.2% for platelet distribution width, and 0.06–0.12% for plateletcrit. LaserCyte equine reference intervals are transferable to the Lusitano horse for 18 of the 22 analytes studied. Regarding age, significant statistical differences were observed for MCV, RDW, neutrophils and lymphocytes between the mean values of young (3–6 years old), middle-aged (7–14 years old) and old (< 15 years old) age groups. MCH means were statistically significantly different between the three age groups. The haematological reference intervals established in this study might represent a valuable and applicable tool for haematological assessment of adult Lusitano horses, providing useful information that helps clinicians to interpret clinical data.

scholarly journals Heamatological parameters of one hump camels (camelusdromedarius) in North Western Nigeria

2021 ◽  
Vol 13 (2) ◽  
pp. 72-78
Author(s):  
H. M. Ibrahim ◽  
I. Bulama ◽  
S. Bashir ◽  
S. I. Abubakar ◽  
W. Ali
Keyword(s):  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Haematological Parameters ◽  
Corpuscular Volume ◽  
Corpuscular Haemoglobin ◽  
Standard Procedures ◽  
Mean Corpuscular Haemoglobin ◽  
Corpuscular Haemoglobin Concentration

Camels are regarded as the ship of the desert, being used for transporting humans and their goods. They are also raised for milk, meat, hides and wool. Normal haematological values are important as disease diagnostic aid as they are vital indicator of the animals’ health status. Haematological parameters of one- humped camels (Camelusdromedarius) were determined in this study. 60 one- humped camels (Camelusdromedarius) were selected in sokoto and their blood samples were analyze using standard procedures for haematological parameters. [Packed cell volume (PCV), red blood cells (RBC), haemoglobin (Hb),white blood cells (WBC), neutrophils, eosinophils, Basophils, monocyte, lymphocyte, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)].For all the parameters, only neutrophils and MCV values showed significant variation between sexes with male camels having higher values than females. There is variation in the range values for all the parameters between sexes except Basophils, Monocyte and Lymphocyte. Based on the results of this study, the values were similar in both sex except in neutrophils and mean corpuscular volume indicating insignificant effect of sex on most of the haematological parameters.

scholarly journals Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Y. Garba ◽  
E. A. Adeola
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Corpuscular Haemoglobin ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Mean Corpuscular Haemoglobin ◽  
Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

scholarly journals INDUCTION OF MACROCYTIC-NORMOCHROMIC ANAEMIA IN Clarias gariepinus –Siluriform- EXPOSED TO SUB-LETHAL CONCENTRATIONS OF 1, 1-DIMETHYL 4, 4-BIPIRIDILLIUM DICHLORIDE (PARAQUAT) UNDER LABPRATORY CONDITIONS

2021 ◽  
Vol 14 (1) ◽  
pp. e3789
Author(s):  
Musa Idi-Ogede Abubakar ◽  
Adeshina Ibrahim
Keyword(s):  
Blood Cells ◽  
Clarias Gariepinus ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Corpuscular Haemoglobin ◽  
Normochromic Anaemia ◽  
Mean Corpuscular Haemoglobin ◽  
L 14 ◽  
Corpuscular Haemoglobin Concentration ◽  
Sublethal Concentrations

Introduction: Macrocytic-normochromic anaemia was induced in Clarias gariepinus at intervals of 1, 7 and 14 days. Experimental fish were exposed to test water separately diluted with sub-lethal concentrations of paraquat of 0, 0.03, 0.05, 0.07 and 0.09mg/L. 14 days. No in-depth work has been carried out on Macrocytic-normochromic anaemia in Nigeria. Objective: to evaluate macrocytic-normochromic anaemia in Clarias gariepinus exposed to sublethal concentrations of paraquat. Methods: Blood were collected at intervals of 1, 7 and 14 days .Results: Blood dyscrasias  was observed with a significant (p<0.05) decrease in haemoglobin, haematocrit, red blood cells, white blood cells, lymphocytes and   monocytes. Mean corpuscular volume (MCV), Mean corpuscular haemoglobin (MCH), Neutrophils, Eosinophil and Basophil increased significantly (p<0.05) with increasing concentrations of the toxicant while Mean corpuscular haemoglobin concentration (MCHC) remained normal. Conclusion: Sublethal concentrations of paraquat induced macrocytic-normochromic anaemia in the exposed Clarias gariepinus.

scholarly journals Haematology of air breathing mud eel, Monopterus cuchia (Hamilton, 1822) of Mymensingh and Kishoreganj districts of Bangladesh

2013 ◽  
Vol 22 (2) ◽  
pp. 127-134
Author(s):  
AFM Nazmus Salehin ◽  
Shankar Chandra Mandal
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Air Breathing ◽  
Corpuscular Haemoglobin ◽  
Significant Difference ◽  
Haematological Profile ◽  
Mean Corpuscular Haemoglobin ◽  
Hb Concentration ◽  
Corpuscular Haemoglobin Concentration

Haematological profile of air breathing mud eel, Monopterus cuchia (Hamilton) from Mymensinghh and Kishoreganj were determined in the present study. Erythrocytes, lymphocytes, monocytes, neutrophils, eosinophils and platelet were evaluated. Significantly higher (p < 0.05) haemoglobin (Hb) concentration was found in air breathing mud eel of Kishoreganj than that of Mymensinghh area. The haematocrit (HCT), erythrocyte sedimentation rate (ESR), total red blood cells (TRBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), total white blood cells (TWBC), lymphocytes, neutrophils, monocytes, eosinophils did not show any significant difference. Findings of the present study showed little variation of haematological profile in air breathing mud eel sampled from Mymensinghh and Kishoreganj. Dhaka Univ. J. Biol. Sci. 22(2): 127-134, 2013 (July)

scholarly journals Impact of asymptomatic Plasmodium falciparum on haematological parameters of pregnant women at first antenatal visit in South-western Nigeria

2018 ◽  
Vol 20 (2) ◽  
Author(s):  
Oluwasola O. Obebe ◽  
Olufarati O. Falohun ◽  
Olaitan O. Olajuyigbe ◽  
Mike A. Lawani ◽  
Olubunmi A. Ajayi
Keyword(s):  
Plasmodium Falciparum ◽  
Pregnant Women ◽  
Blood Cells ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Falciparum Infection ◽  
Antenatal Visit ◽  
Second Trimester ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin

Background: Pregnant women in malaria-endemic areas are at high risk of Plasmodium falciparum infection and its complications. This study investigated the impact of asymptomatic P. falciparum on haematological parameters of pregnant women at first antenatal visit in south-western Nigeria.Methods: Hospital-based cross-sectional evaluation of 130 asymptomatic pregnant women was conducted. Plasmodium infection was diagnosed using Giemsa-stained blood smear microscopy and rapid diagnostic test, while haemoglobin levels (Hb), Packed Cell Volume (PCV), white blood cells (WBC) count, red blood cell (RBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were determined using semi-automated haematology analyser.Results: Multigravid pregnant women presented more during their second trimester. Overall malaria prevalence in the sampled population was 14.6%. Prevalence of malaria parasitaemia was highest (68.4%) in the second trimester. P. falciparum infected pregnant women had significantly lower mean values of packed cell volume (PCV), haematocrit (Hb), and total white blood cell (WBC) compared to non-infected  individuals  (t = -4.07, p= 0.001; t = 3.30, p = <0.001; t = -2.77, p <0.001).Conclusion: There is asymptomatic P. falciparum infection in the study area and this may form a reservoir for transmission. Pregnant women infected with malaria parasites exhibited important changes in haematocrit level, haemoglobin concentration, and total white blood cells. Use of anti-malaria therapy and insecticide-treated bed net would result in greater haematological benefits.

scholarly journals Sexual Dimorphism on Haematological and Genetic Parameters of Japanese Quails

2020 ◽  
pp. 1-11
Author(s):  
J. E. Udoh ◽  
U. H. Udoh ◽  
A. A. Adeoye
Keyword(s):  
Body Weight ◽  
Blood Cell ◽  
Management Practices ◽  
Haemoglobin Concentration ◽  
Haematological Parameters ◽  
Mean Values ◽  
Corpuscular Haemoglobin ◽  
Japanese Quails ◽  
Mean Corpuscular Haemoglobin ◽  
The University

The study evaluated effect of Sex on Haematological parameters and Correlation with body weight of Japanese quails at Teaching and Research Farm of University of Uyo, Uyo, Nigeria. Authors declared that “principle of laboratory animal care” (NIH publication 85-23 revised 1985) were followed as well as the University law. The experiment has been examined and approved by the University law. Total of 400 birds (200 birds per sex) were reared in a Completely Randomized Design under standard management practices for 7 weeks with feed and water given ad libitum. Sex were considered treatments. Weekly body weight (BW) was measured. 100 birds per sex were randomly selected and blood samples were collected for haematological analysis. The parameters include: White Blood Cell(WBC), Red Blood Cell(RBC), Haemoglobin(Hb), Packed Cell Volume(PCV), Mean Corpuscular Volume(MCV), Mean Corpuscular Haemoglobin(MCH), Mean Corpuscular Haemoglobin Concentration(MCHC) and Platelet. The results showed that sex significantly (P< .05) influenced some of the haematological parameters of Japanese quails at 7 weeks. Females had significantly (P<.05) higher mean values in WBC (45.9 ±1.47 ×109/L), Hb (15.58±0.23 g/l) and PCV (47.75±0.72%) than males with WBC (32.09±1.31×109/L), Hb (13.72±0.25 g/l) and PCV (41.50±0.66%). There were no significant (P >.05) difference in mean values for RBC, MCV, MCH, MCHC and Platelet in both sexes measured. Correlation between BW and haematological parameters for male quails ranged from -10% to 50% (low to medium trend) while that of Female counterparts was -19% to 60% (low to high trend). In conclusion, female quails had higher values in WBC, Hb and PVC than the Males counterparts. Hence, female quails were considered fit for studying quails’ health status as this will help in establishing a breeding program of the quails than using Males. Body weight of female quails is a good selection tool to predict haematological parameters of quails.

scholarly journals Haematology values and effect of age and reproductive stage on haematological parameters of Chios sheep

2017 ◽  
Vol 58 (2) ◽  
pp. 124 ◽  
Author(s):  
N. K. PANOUSIS (Ν.Κ. ΠΑΝΟΥΣΗΣ) ◽  
M. KRITSEPI-KONSTANTINOU (Μ. ΚΡΙΤΣΕΠΗ-ΚΩΝΣΤΑΝΤΙΝΟΥ) ◽  
N. D. GIADINIS (Ν.Δ. ΓΙΑΔΙΝΗΣ) ◽  
E. KALAITZAKIS (Ε. ΚΑΛΑΪΤΖΑΚΗΣ) ◽  
Z. POLIZOPOULOU (Ζ. ΠΟΛΥΖΟΠΟΥΛΟΥ) ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Reproductive Stage ◽  
Haematological Parameters ◽  
Mean Values ◽  
Group A ◽  
Chios Sheep ◽  
Effect Of Age

The main aim of the present study was to determine the values of the haematological parameters of dairy sheep reared under intensive and semi-intensive conditions, as well as to test for the effects of age and reproductive stage of the animals on the values of these parameters. In total, 200 clinically healthy Chios sheep from 10 farms of Central Macedonia were used in the study. For the determination of the effect of age 150 sheep were assigned in three groups. Group A consisted of 50 lambs aged 3-6 months (meantSD: 4.45±1.22 months), group Β of 50 non-pregnant ewes into lactation aged 1-3 years (meantSD: 2.18±0.74 years) and group C of 50 non-pregnant ewes into lactation aged more than 3 years (meantSD: 5.88±1.87 years). For evaluating the effect of reproductive status 50 pregnant ewes in dry period were used, 15-30 days before the expected day of lambing (group D), along with the 100 non-pregnant ewes into lactation of groups Β and C (group E). Blood sampling was performed once, in dry ewes from December to January, and in lambs and lactating ewes from March to May. The mean values of the determined haematological parameters were within the range provided by the literature, except for red blood cells count of ewes. The results also showed that haematocrit, haemoglobin concentration and red blood cells, white blood cells, neutrophils, lymphocytes, eosinophils and platelets count were significantly affected by the age; only monocytes and eosinophils count by the reproductive stage of the animals.

scholarly journals Breed-Specific Haematologic Reference Values in Adult Turkeys (Meleagris gallopavo) in the Humid Tropics of Nigeria

2017 ◽  
Vol 61 (1) ◽  
pp. 11-14
Author(s):  
G. Daniel-Igwe ◽  
N. Okwara
Keyword(s):  
Blood Cell ◽  
Reference Values ◽  
Haemoglobin Concentration ◽  
Meleagris Gallopavo ◽  
Humid Tropics ◽  
Haematological Parameters ◽  
Mean Values ◽  
Corpuscular Haemoglobin ◽  
Standard Procedures ◽  
Mean Corpuscular Haemoglobin

Abstract One hundred (50 males and 50 females) B-not strain indigenous turkeys, Meleagris gallopavo, were used to determine the reference values for their haematological parameters. The turkeys were housed in the poultry unit and jugular venepunctures were used to collect their blood. The haematological parameters were determined using standard procedures. The mean values of: the packed cell volume (PCV; 37.29 ± 0.37 %), red blood cell (RBC) counts (2.50 ± 0.44 × 106.µl−1), haemoglobin concentration (Hbc; 10.89 ± 0.34 g.dl−1), mean corpuscular volume (MCV; 150.63 ± 0.73 fl), mean corpuscular haemoglobin (MCH; 44.29 ± 1.78 pg), mean corpuscular haemoglobin concentration (MCHC; 29.10 ± 0.73 g.dl−1), and white blood cell (WBC) counts (12.41 ± 0.83 × 103 µl−1) were determined. No significant differences were found between the male and female B-not strain turkeys in this study. The results will help in the interpretation of cases of disease when there are variations in the values and serve as baseline data for B-not strain of turkeys in the humid tropics.

scholarly journals Haematological parameters and serum biochemistry of Japanese quail chicks (Coturnixcoturnix japonica) fed raw and processed pigeon pea (Cajanus cajan) seed meal based diets

2020 ◽  
Vol 44 (1) ◽  
pp. 89-99
Author(s):  
A. R. Akintunde ◽  
J. J. Omage ◽  
G. S. Bawa ◽  
P. A. Onimisi ◽  
I. Samuel
Keyword(s):  
Blood Cells ◽  
Cajanus Cajan ◽  
Haemoglobin Concentration ◽  
Serum Biochemistry ◽  
Pigeon Pea ◽  
Haematological Parameters ◽  
Seed Meal ◽  
Corpuscular Haemoglobin ◽  
Mean Corpuscular Haemoglobin ◽  
Pea Seed

An experiment was conducted with 720 one - week old Japanese quail chicks to evaluate the effect of feeding raw and processed pigeon pea (Cajanus cajan) seed meal based diets on the haematology and serum biochemistry of the Japanese quails in a completely randomized design. Birds were allocated to eight treatment groups with three replicates each containing 30 birds per replicate. The control diet (TI) had no pigeon pea seed meal, T2 was raw pigeon pea seed while other diets contained 30% of pigeon pea seed meal processed by soaking (for 24, 48 and 72 hours) (T3 – T5), fermentation for 72 hours(T5), boiling for 60 minutes(T6) and roasting for 30 minutes(T7). Blood samples were collected from the birds through the wing  web vein at the 6th week of the feeding trial. The haematological parameters investigated were packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells count (WBC), lymphocytes, neutrophils, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) while serum biochemical components were creatinine, cholesterol, albumin, urea and total protein concentrations. The results obtained in this study showed that all the haematological parameters and serum biochemistry of birds were significantly (P<0.05) influenced by dietary treatments except albumin concentration (g/dL). The packed cell volume, haemoglobin concentration, red blood cells and total protein values were within the range of 41.00 – 52.17 %, 13.63 –17.35g/dl, 6.80 – 8.83 (x 106 /mm3 ) and 5.62 – 7.70 g/dl respectively. The results suggest that raw and processed pigeon pea seed meal (PPSM) possess good dietary protein quality for optimal growth and had no adverse effect on haematological and biochemical profiles of quail chicks at 30 % dietary level of inclusion.

scholarly journals Citarum River Water Pollution and Stress Responses in The Tolerant and Sensitive Fish

2021 ◽  
Vol 2 (1) ◽  
pp. 53-64
Author(s):  
Sunardi Sunardi ◽  
Kabul Fadilah ◽  
Nadia Istiqomah ◽  
Miranti Ariyani ◽  
Desak Made Malini ◽  
...  
Keyword(s):  
Water Pollution ◽  
Stress Responses ◽  
Blood Cells ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
River Pollution ◽  
Blood Parameters ◽  
Corpuscular Haemoglobin ◽  
Stressful Environment ◽  
Mean Corpuscular Haemoglobin

Severe river pollution creates a stressful environment for aquatic organisms, causing disturbances to their health and leading to their extinction. Thus, in this study, the stress responses of tolerant (Oreochromis niloticus) and sensitive (Cyprinus carpio) fish to river pollution in the Citarum River, Indonesia were investigated. The two groups of fish were exposed to varying levels and lengths of water pollution, their blood was sampled, and stress responses were identified. The red blood cells (RBC) count, haemoglobin (HGB), haematocrit (HCT), white blood cells (WBC) count, blood glucose levels, and derived haematological indices such as mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC), were measured. Significant changes in these indices, with tendential changes in RBC and HCT, were observed, indicating that stress responses had occurred in both groups of fish. The sensitive fish recorded more pronounced changes in their haematological activities, with greater amounts of blood parameters being altered in response to water pollution exposure. This result suggested that the sensitive fish were more susceptible to environmental stressors and were, therefore, more vulnerable to extinction in a polluted natural environment.

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