scholarly journals Haematological parameters and serum biochemistry of Japanese quail chicks (Coturnixcoturnix japonica) fed raw and processed pigeon pea (Cajanus cajan) seed meal based diets

2020 ◽  
Vol 44 (1) ◽  
pp. 89-99
Author(s):  
A. R. Akintunde ◽  
J. J. Omage ◽  
G. S. Bawa ◽  
P. A. Onimisi ◽  
I. Samuel

An experiment was conducted with 720 one - week old Japanese quail chicks to evaluate the effect of feeding raw and processed pigeon pea (Cajanus cajan) seed meal based diets on the haematology and serum biochemistry of the Japanese quails in a completely randomized design. Birds were allocated to eight treatment groups with three replicates each containing 30 birds per replicate. The control diet (TI) had no pigeon pea seed meal, T2 was raw pigeon pea seed while other diets contained 30% of pigeon pea seed meal processed by soaking (for 24, 48 and 72 hours) (T3 – T5), fermentation for 72 hours(T5), boiling for 60 minutes(T6) and roasting for 30 minutes(T7). Blood samples were collected from the birds through the wing  web vein at the 6th week of the feeding trial. The haematological parameters investigated were packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells count (WBC), lymphocytes, neutrophils, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) while serum biochemical components were creatinine, cholesterol, albumin, urea and total protein concentrations. The results obtained in this study showed that all the haematological parameters and serum biochemistry of birds were significantly (P<0.05) influenced by dietary treatments except albumin concentration (g/dL). The packed cell volume, haemoglobin concentration, red blood cells and total protein values were within the range of 41.00 – 52.17 %, 13.63 –17.35g/dl, 6.80 – 8.83 (x 106 /mm3 ) and 5.62 – 7.70 g/dl respectively. The results suggest that raw and processed pigeon pea seed meal (PPSM) possess good dietary protein quality for optimal growth and had no adverse effect on haematological and biochemical profiles of quail chicks at 30 % dietary level of inclusion.

2018 ◽  
Vol 66 (4) ◽  
pp. 530-541 ◽  
Author(s):  
Ana Cristina Silvestre-Ferreira ◽  
Mário Cotovio ◽  
Mário Maia ◽  
Felisbina Queiroga ◽  
M João Pires ◽  
...  

The Lusitano horse is an autochthonous Portuguese breed with a growing worldwide expansion. Our objective was to establish reference intervals for haematological parameters using the haematological cell counter LaserCyte (IDEXX). For this purpose, blood samples from 100 healthy adult horses (13 females and 87 males, ranging from 3 to 25 years of age) were analysed. The reference intervals were estimated following the ASVCP guidelines with the Reference Value Advisor software. The obtained reference intervals were 6.4–10.1 × 1012/L for red blood cells, 30.6–45.1% for haematocrit, 11.6–17.1 g/dL for haemoglobin, 42.8–53.2 fL for mean corpuscular volume (MCV), 15.5–20.8 pg for mean corpuscular haemoglobin (MCH), 33.7–39.4 g/dL for mean corpuscular haemoglobin concentration, 17.8–20.3% for red cell distribution width (RDW), 4.5–10.1 × 109/L for white blood cells, 2.2–6.0 × 109/L for neutrophils, 0.9–4.9 × 109/L for lymphocytes, 0.2–0.5 × 109/L for monocytes, 0.1–0.6 × 109/L for eosinophils, 0.0–0.1 × 109/L for basophils, 78.5–172.2 K/mL for platelets, 4.3–9.4 fL for mean platelet volume, 18.8–24.2% for platelet distribution width, and 0.06–0.12% for plateletcrit. LaserCyte equine reference intervals are transferable to the Lusitano horse for 18 of the 22 analytes studied. Regarding age, significant statistical differences were observed for MCV, RDW, neutrophils and lymphocytes between the mean values of young (3–6 years old), middle-aged (7–14 years old) and old (< 15 years old) age groups. MCH means were statistically significantly different between the three age groups. The haematological reference intervals established in this study might represent a valuable and applicable tool for haematological assessment of adult Lusitano horses, providing useful information that helps clinicians to interpret clinical data.


2021 ◽  
Vol 13 (2) ◽  
pp. 72-78
Author(s):  
H. M. Ibrahim ◽  
I. Bulama ◽  
S. Bashir ◽  
S. I. Abubakar ◽  
W. Ali

Camels are regarded as the ship of the desert, being used for transporting humans and their goods. They are also raised for milk, meat, hides and wool. Normal haematological values are important as disease diagnostic aid as they are vital indicator of the animals’ health status. Haematological parameters of one- humped camels (Camelusdromedarius) were determined in this study. 60 one- humped camels (Camelusdromedarius) were selected in sokoto and their blood samples were analyze using standard procedures for haematological parameters. [Packed cell volume (PCV), red blood cells (RBC), haemoglobin (Hb),white blood cells (WBC), neutrophils, eosinophils, Basophils, monocyte, lymphocyte, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)].For all the parameters, only neutrophils and MCV values showed significant variation between sexes with male camels having higher values than females. There is variation in the range values for all the parameters between sexes except Basophils, Monocyte and Lymphocyte. Based on the results of this study, the values were similar in both sex except in neutrophils and mean corpuscular volume indicating insignificant effect of sex on most of the haematological parameters.


Author(s):  
Y. Garba ◽  
E. A. Adeola

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.


2021 ◽  
Vol 8 (27) ◽  
pp. 2434-2438
Author(s):  
Siddegowda M.S ◽  
Chaithra R ◽  
Shivakumar S ◽  
Maithri C.M

BACKGROUND Thyroid hormones play an important role in the regulation and production of red blood cells. Thyroid dysfunction induces different effects on blood cells such as anaemia, erythrocytosis, leucopenia, thrombocytopenia and alteration in red cell indices. In this study, we wanted to compare the changes in haematological parameters of thyroid dysfunction patients with those of euthyroid group. METHODS This was a retrospective study done on 310 individuals by collecting data from the medical records. Later the patients were categorized into hypothyroid (33) thyroid stimulating hormone (TSH > 5.5 μIU/mL), hyperthyroid (19) (TSH < 0.3 μIU/mL) and euthyroid (258) (TSH = 0.3 - 5.5 μIU/ml) groups. The haematological parameters of all these patients were obtained by 5-part automated cell count analyser. Finally, the obtained data was analyzed by statistical package for social sciences (SPSS) software. RESULTS The data obtained from the analysis revealed statistically significant (P < 0.05) difference between hypothyroidism, hyperthyroidism and euthyroidism in mean red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red cell distribution width (RDW), white blood cell (WBC) count and platelet count but the difference was not significant for mean haemoglobin, mean corpuscular haemoglobin concentration (MCHC) (P > 0.05). The mean haemoglobin was lower in hypothyroid patients when compared to euthyroid and hyperthyroid patients. The RBC count (P < 0.007), MCH (P = 0.002) and RDW (P < 0.001) showed statistically significant difference between hypothyroidism and euthyroidism, MCV (P = 0.005) showed statistically significant difference between hyperthyroid and euthyroid groups. CONCLUSIONS In case of patients with abnormal haematological parameters, thyroid hormones evaluation is necessary. KEYWORDS Hypothyroidism, Hyperthyroidism, Haemoglobin, Blood Count, Red Cell Indices


2021 ◽  
Author(s):  
Irena Golinar Oven ◽  
Alenka Nemec Svete ◽  
Melita Hajdinjak ◽  
Jan Plut ◽  
Marina Stukelj

Abstract BackgroundHaematological examination is an important diagnostic tool in the assessment of pig health status. The present study aimed to assess haematological parameters in pigs of different age categories from six farrow-to-finish farms differing in herd health status. The following pig categories were included: 5 age groups of growers (5, 7, 9–10, 11 and 12–13 weeks-old), fatteners and breeding pregnant sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken and group samples of oral fluid and faeces were collected from each animal category in each of the six farms and tested for the detection of Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Virus (PRRSV), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. Individual blood samples were analysed using an automated laser-based haematology analyser. The following haematological parameters were reported: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), and percentage and number of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and "large unstained cells" - LUCs. ResultsOn farms free of PRRSV, PCV2 and HEV, age had significant effect on the following parameters: WBC, Hb, MCH, MCHC, PLT, percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and LUCs. On farms with PRRS, PCV2 and/or HEV, age significantly affected all observed blood parameters except the percentage of LUCs. The percentages of lymphocytes, MCV and Hct were significantly lower by PRRSV while WBC, PLT, percentage and absolute number of neutrophils, basophils and LUCs increased. Significantly lower percentages of lymphocytes and increased percentages and absolute numbers of neutrophils, eosinophils and basophils were caused by PCV2 presence. Significantly lower percentage of lymphocytes and MCV and increased RBC, Hb, percentage and number of basophils and percentage of neutrophils were caused by HEV. Conclusions Alterations of haematological parameters reflected the health status of pigs of different categories on infected and on non-infected farms. Age-related changes in haematological parameters occurred in clinically healthy and in infected pigs.


2017 ◽  
Vol 9 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Onyinyechukwu A. AGINA ◽  
Wilfred S. EZEMA ◽  
Ezinwa M. IWUOHA

The present study established the reference values and sex differences in the erythrocytic and serum biochemistry parameters of domestic adult quails (Coturnix coturnix). Ninety five adult birds, comprising of 42 males and 53 female Japanese quails were sampled using a simple random sampling technique. Standard procedures were carried out in all haematology and serum biochemistry determinations. The overall mean for the erythrocytic and serum biochemistry parameters were as follows: packed cell volume (PCV) 43.11%, red blood cell count (RBC) 4.31 × 106/µl, haemoglobin concentration (Hbc) 16.21 g/dl, mean corpuscular volume (MCV) 100.69 fl, mean corpuscular haemoglobin (MCH) 39.17 pg, mean corpuscular haemoglobin concentration (MCHC) 39.35 g/dl, aspartate aminotransferase (AST) 59.99 IU/L, alanine aminotransferase (ALT) 20.85 IU/L, alkaline phosphatase (ALP) 107.54 IU/L, total proteins (TP) 5.19 g/dl, albumin (ALB) 3.25 g/dl, globulin (GLB) 1.94 g/dl, albumin: globulin 1.73, total cholesterol (TCHOL) 146.69 mg/dl, total bilirubin (TBIL) 2.37 mg/dl, uric acid (UA) 16.02 mg/dl and creatinine (CREAT) 0.44 mg/dl. The PCV of the males were significantly higher than that of the females, while the MCH and MCHC of the females were significantly higher than those of the males. The serum total proteins, albumin, globulin, uric acid, creatinine, and total cholesterol values of the female quails were higher than those of the male quails. The present data might be useful to avian specialists and veterinary clinicians, but more research works should be carried out on quails to increase the information data base, especially in the tropics.


2020 ◽  
Vol 46 (1) ◽  
pp. 149-156
Author(s):  
D. N. Onunkwo ◽  
J. Nathaniel ◽  
J. C. Ezike ◽  
W. Amaduruonye ◽  
G. Daniel-Igwe

This study was carried out to evaluate the effect of garlic rhizome powder on blood profile of broiler at different growth phases (starter and finisher phase). One hundred and twenty Abor acre broiler chicks were used for the study and the chicks were randomly distributed into four treatment groups and the treatment groups were then divided into different replicates with each replicate having 10 chicks i.e. 30 chicks in each treatment group. The garlic powder was included at different inclusion levels and the treatments were designed as T1: basal diet with no garlic powder, T2: basal diet supplemented with 0.5% garlic powder T3: basal diet supplemented with 1.0% garlic powder and T4 having 1.5% garlic powder. Starter diet was offered to the birds for the first four weeks of the feeding trial and finisher diet was also provided for the birds within 5th to 8th week. Daily feed intake and weekly body measurement were taken. At the end of the starter phase, blood was collected for haematological and serum biochemistry analysis also blood was collected for blood profile analysis at the seventh (8th) week of the experiment. The blood samples collected were analyzed for haematological  parameters which include haemoglobin, Packed cell volume, Red blood cells, White blood cells, Mean corpuscular haemoglobin, Mean corpuscular volume, Mean corpuscular haemoglobin concentration, heterophils, lymphocytes, monocytes, eosinophils and serum biochemical parameters which include Aspartate aminotransferase, Alanine aminotransferase, Alkaline Phosphate, Cholesterol, Total protein, Albumin, Globulin, Uric acid and Creatinine. Results showed that the effect of feeding graded levels of garlic rhizome powder on the haematological and serum parameters were significant (P<0.05). The cholesterol levels of experimental birds dropped significantly with increasing levels of garlic in the diets. Inclusion of garlic powder on broiler feed had no significant difference (P>0.05) on the creatinine level. Therefore 1.5% inclusion of garlic should be added to broiler feed to enhance production of broiler lean meat with lowest cholesterol.  


2021 ◽  
Vol 14 (1) ◽  
pp. e3789
Author(s):  
Musa Idi-Ogede Abubakar ◽  
Adeshina Ibrahim

Introduction: Macrocytic-normochromic anaemia was induced in Clarias gariepinus at intervals of 1, 7 and 14 days. Experimental fish were exposed to test water separately diluted with sub-lethal concentrations of paraquat of 0, 0.03, 0.05, 0.07 and 0.09mg/L. 14 days. No in-depth work has been carried out on Macrocytic-normochromic anaemia in Nigeria. Objective: to evaluate macrocytic-normochromic anaemia in Clarias gariepinus exposed to sublethal concentrations of paraquat. Methods: Blood were collected at intervals of 1, 7 and 14 days .Results: Blood dyscrasias  was observed with a significant (p<0.05) decrease in haemoglobin, haematocrit, red blood cells, white blood cells, lymphocytes and   monocytes. Mean corpuscular volume (MCV), Mean corpuscular haemoglobin (MCH), Neutrophils, Eosinophil and Basophil increased significantly (p<0.05) with increasing concentrations of the toxicant while Mean corpuscular haemoglobin concentration (MCHC) remained normal. Conclusion: Sublethal concentrations of paraquat induced macrocytic-normochromic anaemia in the exposed Clarias gariepinus.


2020 ◽  
Vol 42 (1) ◽  
pp. 71-78
Author(s):  
H. O. Amusa ◽  
R. A. Kehinde ◽  
R. O. Ayotebi ◽  
O. A. Abu

A total of fifty Wistar albino rats weighing 50-60g were randomly allocated to five dietary treatments in a completely randomized design to investigate the haematology, serum biochemistry and organ weight changes on diets containing processed dehulled jack bean. Four diets containing 10% crude protein were formulated to contain casein (T1)-control diet, dehulled jack bean soaked in filtrate obtained from 24hr water-steeped whole maize grains (T2), dehulled jack bean soaked in lye solution (T3), dehulled jack bean boiled for 15 minutes (T4) while a protein free diet served as a negative control (T5). The feeding trial lasted for 28days after which four rats per treatments were sampled to evaluate haematological and serum biochemical indices while three rats per treatment were sacrificed by cervical decapitation and the heart, liver, kidneys and the lungs removed and weighed separately. Haemoglobin (Hb), white blood cells (WBC), eosinophil and Mean Corpuscular Haemoglobin Concentration (MCHC) were not affected by dietary treatments while the packed cell volume, red blood cells, lymphocytes, neutrophils, monocytes, Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin (MCH) were significantly affected (P<0.05) by dietary treatments. Rats on T4 had the highest PCV (47.00%), a d neutrophil (25.67%) values, while rat on control had the highest value of RBC 5.95 (×106/mm3). Rats on T2 had the highest lymphocyte (80.33%) and monocyte (3.50%) values, while the lowest value was for rats T4 (73.25%) and T3 (2.00%). Rats on T3 had the highest MCV (1.57fl) and MCH (0.40pg) values the total protein and alkaline phosphatise (ALP) were similar (P>0.05) for all group, while the albumin, globulin, cholesterol, Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) were influence by dietary treatments (P<0.05). Albumin values ranges from 5.83g/dl to 7.68g/dl with rats on T4 having the highest albumin value (7.68g/dl). Globulin value for rats on T4 is significantly (P<0.05) different from rats on T1, T2, T3, and T5 with rats on T3 having the highest value (3.42). Rats on T1 had the highest Cholesterol (55.37mg/dl) and ALT (17.49IU/L) value, while Rats on T2 had the highest AST (16.13IU/L) value. Kidney weights were similar for all group (P>0.05) while the liver, lungs and heart relative weights were significantly affected (P< 0.05) by dietary treatments. Rats on control diet had the lowest relative weight for liver (3.74), lungs (1.03), kidney (0.92) and heart (0.49). Rats on T3 and T5 had the highest value for heart weight (0.75) and liver weight (5.67)while rats on T4 had the highest value for lungs (1.59).Rats fed dehulled jack bean seed soaked in ogi liquor showed slight improvement on haematological, serum profile and organ weight changes over those fed lye soaked jack bean and boiled for 15minutes. However, all the processing methods were ineffective when compared to the performance of rat fed casein based diets.


2013 ◽  
Vol 22 (2) ◽  
pp. 127-134
Author(s):  
AFM Nazmus Salehin ◽  
Shankar Chandra Mandal

Haematological profile of air breathing mud eel, Monopterus cuchia (Hamilton) from Mymensinghh and Kishoreganj were determined in the present study. Erythrocytes, lymphocytes, monocytes, neutrophils, eosinophils and platelet were evaluated. Significantly higher (p < 0.05) haemoglobin (Hb) concentration was found in air breathing mud eel of Kishoreganj than that of Mymensinghh area. The haematocrit (HCT), erythrocyte sedimentation rate (ESR), total red blood cells (TRBC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), total white blood cells (TWBC), lymphocytes, neutrophils, monocytes, eosinophils did not show any significant difference. Findings of the present study showed little variation of haematological profile in air breathing mud eel sampled from Mymensinghh and Kishoreganj. Dhaka Univ. J. Biol. Sci. 22(2): 127-134, 2013 (July)


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