Haematological and biochemical profiles during the puerperium in dairy cows – Short communication

The main aim of the current study was to assess the prevalence of anaemia in Holstein dairy cows during the puerperium, and the haematological and biochemical profile of dairy cows with and without anaemia. The study was conducted in seven dairy herds in São Paulo State, Brazil. The evaluated sample comprised a total of 336 Holstein cows. Blood samples were collected at postpartum day 25 ± 3. Haematological analysis included white blood cell, red blood cell and platelet count, haematocrit value, haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. The biochemical profile encompassed cholesterol, non-esterified fatty acids, β-hydroxybutyrate, albumin, globulin, fibrinogen, calcium and total bilirubin concentrations. The prevalence of anaemia was 16.3% in all herds, and this was not affected by clinical diseases, milk production, parity and body score condition. Moreover, anaemic cows had lower red blood cell count, haematocrit, haemoglobin, serum cholesterol and calcium concentrations and higher white blood cell and platelet counts, mean corpuscular haemoglobin, red cell distribution width, non-esterified fatty acids, β-hydroxybutyrate, fibrinogen and globulin concentrations when compared with non-anaemic cows. The results indicate changes in energy balance and an inflammatory process in anaemic cows.

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Haematological Profiles of Pigs on Different Farms Reflect Their Health Status

10.21203/rs.3.rs-644732/v1 ◽

2021 ◽

Author(s):

Irena Golinar Oven ◽

Alenka Nemec Svete ◽

Melita Hajdinjak ◽

Jan Plut ◽

Marina Stukelj

Keyword(s):

Health Status ◽

Blood Cell ◽

Cell Count ◽

White Blood Cell ◽

Haemoglobin Concentration ◽

Haematological Parameters ◽

Blood Cell Count ◽

Blood Samples ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin

Abstract BackgroundHaematological examination is an important diagnostic tool in the assessment of pig health status. The present study aimed to assess haematological parameters in pigs of different age categories from six farrow-to-finish farms differing in herd health status. The following pig categories were included: 5 age groups of growers (5, 7, 9–10, 11 and 12–13 weeks-old), fatteners and breeding pregnant sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken and group samples of oral fluid and faeces were collected from each animal category in each of the six farms and tested for the detection of Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Virus (PRRSV), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. Individual blood samples were analysed using an automated laser-based haematology analyser. The following haematological parameters were reported: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), and percentage and number of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and "large unstained cells" - LUCs. ResultsOn farms free of PRRSV, PCV2 and HEV, age had significant effect on the following parameters: WBC, Hb, MCH, MCHC, PLT, percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and LUCs. On farms with PRRS, PCV2 and/or HEV, age significantly affected all observed blood parameters except the percentage of LUCs. The percentages of lymphocytes, MCV and Hct were significantly lower by PRRSV while WBC, PLT, percentage and absolute number of neutrophils, basophils and LUCs increased. Significantly lower percentages of lymphocytes and increased percentages and absolute numbers of neutrophils, eosinophils and basophils were caused by PCV2 presence. Significantly lower percentage of lymphocytes and MCV and increased RBC, Hb, percentage and number of basophils and percentage of neutrophils were caused by HEV. Conclusions Alterations of haematological parameters reflected the health status of pigs of different categories on infected and on non-infected farms. Age-related changes in haematological parameters occurred in clinically healthy and in infected pigs.

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Nutritional evaluation of mixed forages (Panicum maximum and Calopogonium mucunoid) as feed resources on the haematological indices of pigs

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i4.1004 ◽

2020 ◽

Vol 46 (4) ◽

pp. 206-210

Author(s):

H. O. Uzegbu ◽

I. Annatte ◽

D. Onunkwo ◽

E. K. Ndelekwute ◽

E. Ewa

Keyword(s):

Blood Cell ◽

Cell Volume ◽

White Blood Cell ◽

Packed Cell Volume ◽

Haemoglobin Concentration ◽

Panicum Maximum ◽

Corpuscular Haemoglobin ◽

Feed Resources ◽

Randomized Design ◽

Mean Corpuscular Haemoglobin

A feed trial was conducted with a total of 30 pigs to evaluate the potential of mixed fresh forages (Panicum maximum and Calopogonium mucunoids) on pigs. The trial lasted for 63 days. Five diets were formulated which contains 0, 10, 15, 20 and 25% level of grass and legume forage. Each diet formed a treatment which was offered to the pigs. Each treatment was replicated thrice with 2 pigs per replicate, arranged in a completely randomized design (CRD). The pigs were fed 4% of their body weight and water was given ad-libitum. The blood composition of the pigs in terms of packed cell volume, haemoglobin, white blood cell, mean corpuscular haemoglobin and mean corpuscular concentration. Results showed significant differences (P<0.05) in white blood cell (17.70 – 23.30) and mean corpuscular heamoglobin (15.83 – 18.03) and mean corpuscular volume (48.00 – 53.16(mg/100ml) and there is no treatment effect on observed in packed cell volume (36.00 – 42.00%), haemoglobin (12.00 – 14.00) and mean corpuscular haemoglobin concentration (33.25 – 33.41%). Recommended level of inclusion of the mixed forage (Panicum maximum and Calopogonium mucunoids) in the diet of pigs should not exceed 20%.

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Stability of Commonly Used Hematological Parameters in Samples Stored at 33°C, 22°C and 4°C

10.1101/148601 ◽

2017 ◽

Author(s):

Ashish Jain ◽

Sanchit Jain ◽

Neha Singh ◽

Priyanka Aswal ◽

Shweta Pal ◽

...

Keyword(s):

Blood Cell ◽

Cell Count ◽

White Blood Cell Count ◽

White Blood Cell ◽

Red Blood Cell ◽

Blood Cell Count ◽

Corpuscular Haemoglobin ◽

Platelet Counts ◽

Red Blood Cell Count ◽

Mean Corpuscular Haemoglobin

AbstractAimThis study aimed to investigate the analytical bias and imprecision in haematological parameters induced by storage at 4°C, 22°C and 33 °C.MethodsThree K2EDTA anticoagulated vials of blood were collected from each of twenty blood donors and stored at 4°C, 22°C and 33°C respectively. Readings from each vial were taken at 0, 4, 6, 12, 24, 48 and 72 hours after collection on the Sysmex XP-100 analyser. The mean and median shift of the parameters relative to the baseline and the coefficient of variation for each time-temperature combination were calculated. The shift was compared to the maximum acceptable bias.ResultsHaemoglobin, Red Blood Cell Count, White Blood Cell Count, Mean Corpuscular Haemoglobin were stable for at least twenty four hours at 33°C. Haematocrit, Mean Corpuscular Volume and Platelet Counts were stable for less than four hours at 33°C. All the above parameters were stable for longer at 22°C and 4°C. The three-part differential count showed instability within four hours at 33 °C.ConclusionsStrict pre-analytical control is needed at 33°C or above due to the marked instability of most parameters. However, Haemoglobin, Red Blood Cell Count, White Blood Cell Count and Mean Corpuscular Haemoglobin remain relatively stable even at 33°C.Key MessageHaematology samples exposed to temperatures of 33°C or above show rapid change in MCV, HCT,MCHC, RDW, Platelet Counts and three-part differential counts. Settings where prolonged exposure to these temperatures cannot be avoided should rely on the more stable parameters of Haemoglobin, RBC Counts, MCH and WBC Counts.

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Hematology of Wild Allied Rock-Wallabies, Petrogale-Assimilis Ramsay, 1877 (Marsupialia, Macropodidae), in North Queensland

Australian Journal of Zoology ◽

10.1071/zo9920355 ◽

1992 ◽

Vol 40 (4) ◽

pp. 355 ◽

Cited By ~ 15

Author(s):

PBS Spencer ◽

R Speare

Keyword(s):

Blood Cell ◽

Cell Count ◽

Cell Volume ◽

Red Blood Cell ◽

Packed Cell Volume ◽

Haemoglobin Concentration ◽

Blood Cell Count ◽

Corpuscular Haemoglobin ◽

Red Blood Cell Count ◽

Mean Corpuscular Haemoglobin

The haematology of a colony of wild allied rock-wallabies, Petrogale assimilis Ramsay, 1877, from north-western Queensland was studied for 20 months. Wallabies were repeatedly trapped at monthly intervals and 511 blood samples from 96 animals were examined, making this the most comprehensive study of the haematology of any macropod species. The morphology of the red blood cells and leucocytes was similar to those of other marsupials. Large and small lymphocytes were recognised. Binucleate lymphocytes were found in 7.2% of blood smears. Normal haematological values were within the published ranges for other marsupials. However, the average white blood cell count (mean +/- s.e.: 9.03 +/- 0.92 X 10(9) L-1) was close to the upper end of the range for macropods. The normal (mean +/- s.e.) red blood cell count (5.24 + 0.15 X 10(12) L-1), packed cell volume (0.4291 +/- 0.019 L L-1) and haemoglobin concentration (14.07 +/- 0.41 g dL-1) of male wallabies were higher than those of females (4.79 +/- 0.15 X 10(12) L-1, 0.3952 +/- 1.19 L L-1, and 12.95 +/- 0.41 g dL-1, respectively). No gender differences were detected in the white blood cell count (9.03 +/- 0.92 x 10(9) L-1), mean corpuscular volume (82.28 +/- 1.35 fL), mean corpuscular haemoglobin (26.98 +/- 0.57 pg) or mean corpuscular haemoglobin concentration (32.82 +/- 0.57 g L 1). The red blood cell count, packed cell volume, haemoglobin concentration, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and the absolute neutrophil count in juveniles and subadults increased with age. The neutrophil to lymphocyte ratio was less than 1.0 in pouch young and approached 1.0 in adults. The red blood cell count, packed cell volume and haemoglobin concentration of lactating females did not vary with age of pounch young.

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Ecotoxicological assessment of commercial glyphosate-based di-ammonium salt formulation on haematological indices of Clarias gariepinus

Journal of Aquatic Sciences ◽

10.4314/jas.v36i1.2 ◽

2021 ◽

Vol 36 (1) ◽

pp. 13-21

Author(s):

A.B. Ella ◽

E.T. Azua ◽

C.U. Aguoru ◽

A.A. Onekutu ◽

F.A. Ella

Keyword(s):

Blood Cell ◽

Clarias Gariepinus ◽

Haemoglobin Concentration ◽

Negative Consequences ◽

Ecotoxicological Assessment ◽

Corpuscular Haemoglobin ◽

Physico Chemical ◽

Haematological Profile ◽

Mean Corpuscular Haemoglobin ◽

Total Dissolved Solid

The study evaluated sub-lethal toxicity of commercial glyphosate-based Di-ammonium formulation commonly known as ‘Touchdown’ on haematological profile of Clarias gariepinus juveniles. A total of two hundred catfish juveniles of mean weight 31.07±1.23g and mean length 19.50 ± 0.50cm were exposed to different concentrations of Di-ammonium formulation (1.26, 2.52, 5.03 and 0.00 mg/l) for eight (8) weeks. Each concentration was treated in triplicate using a static bioassay system. Physico-chemical parameters of the test water were monitored throughout the study period. Results showed that haematocrit (HCT), red blood cell (RBC), haemoglobin (HGB), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC) and Platelets (PLT) counts decreased significantly (p<0.05) with increase in concentrations of Touchdown. Whereas, white blood cell (WBC) counts increased significantly (p<0.05) with increasing concentrations of the glyphosate-based formulation. Water temperature, pH, Total dissolved solid (TDS) and electrical conductivity (EC) increased linearly with increasing concentration, while dissolved oxygen content decreased significantly (p<0.05). The results from this study indicated that Di-ammonium glyphosate–based formulation had negative consequences on the blood of treated fish. In view of this, the application of Di-ammonium glyphosate-based formulation should be done in a sustainable manner with proper regulations.

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Values of Selected Haematological Indicators in Six Species of Feathered Game

Acta Veterinaria Brno ◽

10.2754/avb201079s9s003 ◽

2010 ◽

Vol 79 (9) ◽

pp. S3-S8 ◽

Cited By ~ 4

Author(s):

Eva Straková ◽

Pavel Suchý ◽

Romana Kábelová ◽

František Vitula ◽

Ivan Herzig

Keyword(s):

Blood Cell ◽

Total Concentration ◽

Haemoglobin Concentration ◽

Individual Species ◽

Neutrophil Granulocytes ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin Concentration ◽

Mean Corpuscular Haemoglobin ◽

The Mean ◽

Corpuscular Haemoglobin Concentration

The present paper extends the knowledge of selected blood indicators in six species of feathered game such as helmeted guineafowl (Numida meleagris), common pheasant (Phasianus colchicus), Japanese quail (Coturnix coturnix japonica), chukar (Alectoris chucar), grey partridge (Perdix perdix) and wild turkey (Meleagris gallopavo), reared in the Czech Republic and Europe. The red blood cell (RBC) values in individual species of feathered game ranged as follows: the mean RBC counts varied in a range of 2.10 - 2.58 T/l; haematocrit values were in a range of 0.33 - 0.39 1/1; haemoglobin concentration varied in a range of 101.20 - 129.13 g/l; mean corpuscular haemoglobin concentration (MCHC) ranged from 28.63 to 35.44%; mean corpuscular haemoglobin (MCH) varied in a range of 46.15 - 62.07 pg and the mean corpuscular volume (MCV) was 147.66 - 178.20 fl. Except for the total concentration of haemoglobin and the mean corpuscular haemoglobin concentration, no significant differences were found between the mean values of RBC values in individual species of feathered game. However, some differences between species were found in white blood cell (WBC) values, ranging as follows: the total WBC count ranged from 13.55 to 26.90 G/l, basophil granulocytes from 0.011 to 0.110 G/l, eosinophil granulocytes from 0.011 to 0.593 G/l, neutrophil granulocytes from 1.868 to 6.661 G/l, lymphocytes from 10.937 to 20.922 G/l and monocytes from 0.034 to 0.199 G/l. Most values showed significant (P ⪬ 0.05) to highly significant (P ⪬ 0.01) interspecies differences. The main goal of the study was to investigate selected haematological indicators in six major species of feathered game.

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Evaluation Of Biochemical, Hematological And Antioxidant Properties In Mice Exposed To A Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular

10.1101/2020.08.02.233635 ◽

2020 ◽

Author(s):

S. Kehinde ◽

S. M. Adebayo ◽

A. L. Adesiyan ◽

E. A. Kade ◽

K. Gurpreet

Keyword(s):

Blood Cell ◽

Protein Content ◽

Carica Papaya ◽

Nigella Sativa ◽

Haemoglobin Concentration ◽

High Dose ◽

Corpuscular Haemoglobin ◽

Cell Counts ◽

Mean Corpuscular Haemoglobin ◽

Boswellia Sacra

AbstractNigella sativa, Carica papaya and Boswellia sacra are medicinal plants in the commonly used in folkloric medicine due to the presence of its immense therapeutic properties. Fifty (50) female albino mice weighing between 15-22g were divided into five groups of 10 mice each. Animal in group 1 served as control group and were administered distilled water while animal in group 2 were given 2ml of cisplatin (orally). Animal in group 3-5 were given orally; 100 mg/kg (low dose), 200 mg/kg (medium dose) and 400 mg/kg (high dose) of triherbal preparation. The feeding regimens lasted for 28 days. After 28 days, mammary gland and blood samples were collected for haematological and antioxidant analysis. The triherbal formula decreased the GSH and MDA levels of mice treated with 100 mg/kg and 400 mg/kg doses compare to control. The measurement of total protein content, SOD and CAT increased in treated animals compared to control. However, RBC (Red Blood Cell) counts significantly decreased in the low, medium and high dose groups (0.95±0.08, 6.57±0.08 and 3.55±0.55 x 106 cells/mm3 respectively) compared to control (7.34±0.40) at P<0.05. Also, significant decreases (P<0.05) in the level of the total WBC (White Blood Cell) count, platelet count, PCV (Packed Cell Volume) and Hb (haemoglobin) concentration were observed. The decreases were dose dependent. The MCH (Mean Corpuscular Haemoglobin) and MCHC (Mean Corpuscular Haemoglobin Concentration) except MCV (Mean Corpuscular Volume) significantly decreased in treated group only. The triherbal formulation exhibited significant antioxidant activities showing increased levels of SOD, CAT and Protein content due to activation of the enzyme involve in detoxification of free radicals and decreased in the level of GSH and MDA due to accumulation of peroxides and H2O2. Also, decreased in haematological parameters due to the presence of phytochemicals such as phenol, resins, saponins, sterols, tannis and terpenes in the triherbal formula. Therefore, it has potential to induce haematotoxicity hence consumption of high concentrations should be discouraged.

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Prevalence of Anaplasma ovis and its effects on haematology of apparently healthy Sahel goats in Maiduguri, Nigeria: A Preliminary study

Savannah Veterinary Journal ◽

10.36759/svj.2017.006 ◽

2018 ◽

pp. 53-57

Keyword(s):

Blood Cell ◽

Cell Count ◽

Haemoglobin Concentration ◽

Well Being ◽

Economic Losses ◽

Blood Cell Count ◽

Anaplasma Ovis ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin ◽

Apparently Healthy

Introduction: Anaplasma ovis is an intraerythrocytic rickettsial pathogen that causes clinical symptoms and huge economic losses in flocks. This study was designed to determine the prevalence and effects of Anaplasma ovis on the haematology of apparently healthy Sahel goats in Maiduguri. Methods: Blood samples were randomly collected from one hundred (100) Sahel goats from both farm and abattoir in the study area. Parasitological and haematological analyses were carried out on the collected samples using a standard technique. Results: Out of 100 goats sampled, seven (7) were positive with an overall prevalence of 7%. The prevalence was higher in the male 5 (5%) than in the female 2 (2%) with no significant association between sexes (p > 0.05). The haematological parameters of infected and uninfected goats were within normal range except for mean corpuscular volume (MCV) of infected goats. The MCV of infected and uninfected goats varied significantly (p < 0.05) while the packed cell volume, haemoglobin concentration, red blood cell count, white blood cell count, mean corpuscular haemoglobin concentration and mean corpuscular haemoglobin of infected and uninfected goats were not significantly (p > 0.05) affected. Significance: Natural infection in apparently healthy goats in Maiduguri was found not to have an obvious effect on the haematology of the infected animals. There is a need for prevention and control programs against this pathogen in the study area. This, when carried out, will improve the production potential of these animals and economic well-being of the owners.

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Hematological Changes in Dogs Affected with Canine Babesiosis

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.v12i3.7080 ◽

2017 ◽

Vol 12 (3) ◽

Author(s):

A. K. Bilwal ◽

G. C. Mandali ◽

A. C. Patel

Keyword(s):

Blood Cell ◽

Haemoglobin Concentration ◽

Canine Babesiosis ◽

Corpuscular Haemoglobin ◽

Microcytic Hypochromic Anaemia ◽

Hypochromic Anaemia ◽

Mean Corpuscular Haemoglobin ◽

Wbc Count ◽

Hematological Changes ◽

Hematological Evaluation

Canine babesiosis caused by tick-borne organisms of the genus Babesia, is one of the most significant disease worldwide. The aim of this study was to determine the haematological changes in dogs naturally infected with Babesia. The hematological evaluation included haemoglobin (Hb), red blood cell (RBC) count, platelets, white blood cell (WBC) count, differential leucocyte count (DLC), packet cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). The haematological findings in most of these dogs revealed microcytic hypochromic anaemia, lowered platelet count and 75% cases had 24% decreased PCV. The results of the study showed that Babesia infection in dogs caused anaemia and thrombocytopenia.

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Zinc ameliorates lead toxicity by reducing body Pb burden and restoring Pb-induced haematological and biochemical derangements

Toxicology Research and Application ◽

10.1177/2397847320956562 ◽

2020 ◽

Vol 4 ◽

pp. 239784732095656

Author(s):

Emmanuel Ike Ugwuja ◽

Nweze Vincent ◽

Ikechukwu C Ikaraoha ◽

Samuel R Ohayi

Keyword(s):

Blood Cell ◽

Cell Count ◽

Haemoglobin Concentration ◽

Lead Toxicity ◽

Oral Route ◽

Albino Rats ◽

Blood Cell Count ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin ◽

Biochemical Analyses

Background: Studies on nutritional factors, including zinc in ameliorating the deleterious effects of potentially toxic elements have been scarce and the findings have been inconsistent. Aim: The aim of the present study was to evaluate the ameliorative potential of zinc against lead-induced toxicity in rats. Materials and methods: Male albino rats (n = 24) assigned into four groups of six/group: normal control (NC), zinc control (ZnC; 20 mg/kg ZnCl2), lead control (PbC; 50 mg/kg PbCl2) and lead plus zinc (Pb + Zn; 50 mg/kg PbCl2 plus 20 mg/kg ZnCl2), respectively were investigated. All administrations were through oral route and lasted for 42 days after which blood samples were collected for haematological and biochemical analyses using standard techniques. Results: Results showed that packed cell volume (PCV), haemoglobin concentration (HBC), red blood cell count (RBC) were significant (p < 0.05) reduced while total white blood cell count (TWBC), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and platelets were significantly elevated in PbC group in comparison with NC and ZnC. Also liver and renal function parameters as well as the liver and kidney malondialdehyde (MDA) were elevated while antioxidant enzymes; catalase (CAT) and superoxide dismutase (SOD) were significantly (p < 0.05) reduced in PbC group in comparison with NC and ZnC. Concentrations of lead were in the order: blood > liver > kidney in the PbC group. In addition to restoration of the altered parameters, administration of zinc in Pb + Zn group significantly reduced the raised lead concentrations in the plasma and organs. Conclusion: Reduction in body Pb burden and restoration of Pb-induced derangements in haematological and biochemical parameters by Zn strongly support the ameliorative property of Zn against Pb-induced toxicity.

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