scholarly journals HPLC–MS/MS profiling of wild-growing scentless chamomile

2020 ◽  
Vol 32 (2) ◽  
pp. 86-94 ◽  
Author(s):  
Filip Šibul ◽  
Dejan Orčić ◽  
Sanja Berežni ◽  
Goran Anačkov ◽  
Neda Mimica-Dukić
Keyword(s):  
High Performance ◽  
Quinic Acid ◽  
Human Consumption ◽  
Plant Metabolites ◽  
Liquid Chromatography Tandem Mass ◽  
Health Promoting ◽  
Tandem Mass Spectroscopy ◽  
The Common ◽  
Characteristic Fragmentation ◽  
Fragmentation Patterns

Scentless chamomile (Tripleurospermum inodorum = M. inodora) is a plant belonging to Anthemideae tribe of Asteraceae family, with phenotype similar to the common chamomile, a plant used in human consumption in the form of herbal tea infusion. In order to be able to understand possible health-promoting properties and adverse effects of the scentless chamomile's consumption, it is of essence to examine its chemical composition. The aim of the study was to perform phenolic profiling using high-performance liquid chromatography–tandem mass spectroscopy (HPLC–MS/MS), in comparison to the common chamomile. In the investigated extracts, qualitative and quantitative analyses enabled the identification of 66 compounds based on their retention times, mass (MS/MS) spectra, and analysis of their characteristic fragmentation patterns in MS/MS Product Ion Scan experiments. A new HPLC–MS/MS method for quantitation of common plant metabolites was hereby developed, enabling quantitation of 47 compounds. All examined M. inodora samples have relatively high combined phenolic and flavonoid contents (25.2–51.9 mg/g). Apigenin, apigenin-7-O-glucoside, luteolin, luteolin-7-O-glucoside, quinic acid, and 5-O-caffeoyl quinic acid were the compounds with highest concentration in both inodorous and common chamomile. The results obtained hereby represent the first and most detailed chemical profile of scentless chamomile so far.

scholarly journals Characterization and identification of flavonoids from Bambusa chungii leaves extract by UPLC-ESI-Q-TOF-MS/MS

2020 ◽  
Author(s):  
Ting Yuan ◽  
Xue-Feng Guo ◽  
Si-Yue Shao ◽  
Rong-Miao An ◽  
Jin Wang ◽  
...  
Keyword(s):  
High Performance ◽  
Gradient Elution ◽  
High Definition ◽  
Bamboo Leaves ◽  
Fat Reduction ◽  
Accurate Mass Measurements ◽  
Acid Aqueous Solution ◽  
Characteristic Fragmentation ◽  
Fragmentation Patterns ◽  
Tof Ms

AbstractBamboo leaves extract (BLE) has a variety of physiological functions such as antitumour, anti-inflammatory, antioxidant and blood fat reduction activities and the flavonoids of bamboo leaves are the major active constituents. To profile the flavonoids in the complex BLE, a rapid and sensitive analytical method based on ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) was developed for the structural identification of the flavonoids in Bambusa chungii leaves extract using accurate mass measurements and characteristic fragmentation patterns. After separation on an Agilent SB-C18 Rapid Resolution High Definition (RRHD) column (2.1 mm × 150 mm, 1.8 μm) by gradient elution with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase, the sample was analysed by ESI-QTOF-MS/MS in the negative mode. A total of 22 flavonoids were detected, and eight of these were identified by comparison with reference standards, while the other fourteen were tentatively identified according to their MS/MS data. The main fragmentation pathways of flavonoid C-glycosides (compounds 1, 5 and 10), flavonoid di-C,O-glycosides (compound 4), flavonoid di-C-glycosides (compound 7) and flavonoid C,O-di-glycosides (compounds 2 and 14) are shown in this article. This is the first report on the analysis of the flavonoids in the extract of B. chungii leaves. The present work demonstrates that UPLC-ESI-Q-TOF-MS/MS is an efficient technique for identifying multiple flavonoids of BLE.

IDENTIFICATION OF OLIGOSTILBENES FROM Dipterocarpus semivestitus THROUGH DEREPLICATION TECHNIQUE

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Rohaity Ramli ◽  
Nor Hadiani Ismail ◽  
Nurhuda Manshoor
Keyword(s):  
Liquid Chromatography ◽  
Mass Spectroscopy ◽  
Published Data ◽  
Tandem Mass ◽  
Separation Techniques ◽  
Mass Fragmentation ◽  
Liquid Chromatography Tandem Mass ◽  
Tandem Mass Spectroscopy ◽  
Fragmentation Patterns

Three oligostilbenes have been identified in stems extract of Dipterocarpus semivestitus through dereplication strategy. The stems extract was injected to liquid chromatography tandem mass spectroscopy. Two tetramer, hopeaphenol and hemsleyanol D, were identified by comparing their mass fragmentation patterns with in-house library. A trimer, a-viniferin was isolated by various separation techniques and its structure was confirmed by NMR analyses and comparison with published data.

scholarly journals Determination and Confirmation of Tulathromycin Residues in Bovine Liver and Porcine Kidney via Their Common Hydrolytic Fragment Using High-Performance Liquid Chromatography/Tandem Mass Spectrometry

2011 ◽  
Vol 94 (2) ◽  
pp. 436-445 ◽  
Author(s):  
Pamela L Boner ◽  
David W Gottschall ◽  
Heasook Kim-Kang
Keyword(s):  
Acid Treatment ◽  
High Performance ◽  
Bovine Liver ◽  
Porcine Kidney ◽  
Strong Cation Exchange ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Confirmatory Assay ◽  
The Common

Abstract An accurate, reliable, and reproducible analytical method using HPLC/MS/MS for the determination of tulathromycin residues in bovine liver and porcine kidney via their common hydrolytic fragment (CP-60,300) was developed and validated. Briefly, the method involved an initial acid treatment of intact tissues, which yielded the common fragment (CP-60,300). A portion of the acid hydrolyzate was purified by SPE using a strong cation exchange cartridge. Evaporation of the purified extract was followed by reconstitution in aqueous buffer and analysis by HPLC/MS/MS under isocratic conditions. The developed method provided acceptable sensitivity for determinative surveillance of tulathromycin in porcine kidney and bovine liver with an LOQ of 7.50 and 2.75 g/g, respectively. The overall recovery and precision (CV) of 45 determinations of each tissue were 97.8 (5.3) for porcine kidney and 96.9 (7.9) for bovine liver. Accuracy, precision, linearity, specificity, and ruggedness were demonstrated. An HPLC/MS/MS method was also developed for use in these tissues as a confirmatory assay following modifications to the MS detection parameters. The confirmatory method demonstrated acceptable sensitivity for confirmatory evaluation of tulathromycin in porcine kidney and bovine liver at tolerances of 15 and 5.5 g/g, respectively.

scholarly journals In Vitro Bioaccessibility of Extractable Compounds from Tannat Grape Skin Possessing Health Promoting Properties with Potential to Reduce the Risk of Diabetes

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1575
Author(s):  
Adriana Maite Fernández-Fernández ◽  
Amaia Iriondo-DeHond ◽  
Tiziana Nardin ◽  
Roberto Larcher ◽  
Eduardo Dellacassa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glucose Metabolism ◽  
High Performance ◽  
Glucose Transporters ◽  
Glucose Absorption ◽  
Grape Skin ◽  
Liquid Chromatography Tandem Mass ◽  
In Vitro Bioaccessibility ◽  
Health Promoting

Diabetes pathogenesis encompasses oxidative stress, inflammation, insulin malfunctioning and partial or total insulin secretion impairment, which leads to a constant hyperglycemia. Polyphenols are known to possess bioactive properties, being Tannat grape skin a natural and sustainable source of these compounds. The present study aimed to find out the bioaccessibility of health-promoting molecules composing a multifunctional extract from Tannat grape skin obtained under hydro-alcoholic-acid conditions. The identification of phenolic compounds in the samples was performed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Subsequently, the samples were in vitro digested mimicking the human oral gastrointestinal conditions and the bioactivity of the digest (antioxidant, anti-inflammatory and modulation of glucose metabolism) was assessed. Effect on glucose metabolism was estimated by measuring carbohydrases activity and the functionality of glucose transporters of small intestine cells in presence and absence of the digested extract. Flavonoids, phenolic acids and phenolic alcohols were the major phenol compounds detected in the extract. The bioaccessible compounds protected the intestinal cells and macrophages against the induced formation of reactive oxygen species (ROS) and nitric oxide (NO). In addition, glucose transporters were inhibited by the digested extract. In conclusion, the bioaccessible compounds of the extract, including phenols, modulated key biochemical events involved in the pathogenesis of diabetes such as oxidative stress, inflammation and glucose absorption. The extract was effective under prevention with co-administration conditions supporting its potential for either reducing the risk or treating this disease.

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