scholarly journals First record of the semi-slug Omalonyx unguis (d’Orbigny, 1837) (Gastropoda, Succineidae) in the Misiones Province, Argentina

Check List ◽  
2018 ◽  
Vol 14 (4) ◽  
pp. 705-712
Author(s):  
Leila B. Guzmán ◽  
Enzo N. Serniotti ◽  
Roberto E. Vogler ◽  
Ariel A. Beltramino ◽  
Alejandra Rumi ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Morphological Characteristics ◽  
First Record ◽  
Coi Gene ◽  
Cytochrome Oxidase Subunit ◽  
Specific Identification ◽  
Molluscan Fauna ◽  
Misiones Province ◽  
Species Specific ◽  
Northeastern Argentina

Omalonyx unguis (d’Orbigny, 1837) is a semi-slug inhabiting the Paraná river basin. This species belongs to Succineidae, a family comprising a few representatives in South America. In this work, we provide the first record for the species from Misiones Province, Argentina. Previous records available for Omalonyx in Misiones were identified to the genus level. We examined morphological characteristics of the reproductive system and used DNA sequences from cytochrome oxidase subunit I (COI) gene for species-specific identification. These new distributional data contribute to consolidate the knowledge of the molluscan fauna in northeastern Argentina.

Diversity of shallow water zoantharians in Hengam and Larak Islands, in the Persian Gulf

2015 ◽  
Vol 96 (5) ◽  
pp. 1145-1155 ◽  
Author(s):  
Atoosa Noori Koupaei ◽  
Pargol Ghavam Mostafavi ◽  
Jalil Fallah Mehrabadi ◽  
Seyed Mohammad Reza Fatemi ◽  
Hamed Dehghani
Keyword(s):  
16S Rdna ◽  
Dna Sequences ◽  
Persian Gulf ◽  
Morphological Characteristics ◽  
First Record ◽  
Species Group ◽  
Coi Gene ◽  
Undescribed Species ◽  
External Appearance ◽  
The Persian Gulf

Zoantharians are one of the least studied orders of benthic cnidarians of the Persian Gulf. A survey and molecular analysis was conducted to determine zoantharian species diversity in the Persian Gulf. For this purpose, 63 colonies of zoantharians were collected from Hengam and Larak Islands in the Strait of Hormuz and some morphological characteristics of each specimen were recorded, i.e. sand encrustation, polyp shape and colour, oral groove, oral zone and oral disc colours as well as tentacle number and colour. After DNA extraction, mitochondrial 16S ribosomal DNA (16S rDNA) and cytochrome oxidase subunit I (COI) were amplified by polymerase chain reaction (PCR). Based on obtained 16S rDNA and COI gene sequences, five putative species-level clades were identified:Zoanthus sansibaricus(N = 30),Palythoa tuberculosa(N = 12),Palythoa mutuki(N = 2),Palythoaaff.mutuki(N = 18) andNeozoanthussp. Iran (N = 1). While the first three are known species, the last two were potentially novel undescribed species.Palythoaaff.mutukihas an external appearance similar toPalythoa mutuki. However, mitochondrial DNA sequences obtained from these specimens placed them in a previously undescribed species group. TheNeozoanthusspecimen was morphologically and molecularly different from other describedNeozoanthusspecies. This is the first record of this genus from the Persian Gulf and neighbouring areas. Since there is not much work on zoantharians identification in the Persian Gulf, further sampling and investigation is needed to speculate on the accuracy of these potentially new species and to complete the knowledge of zoantharian diversity in this area.

scholarly journals Development of species-specific Cichla species eDNA primers for alien invasive species (AIS) monitoring in Malaysia

2021 ◽  
Vol 4 ◽  
Author(s):  
O. Nurul Fizatul Nabilah ◽  
A. R. Ramizah ◽  
A. B. Adibah ◽  
S. Syazwan ◽  
A.G. Intan Faraha ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Environmental Dna ◽  
Coi Gene ◽  
Alien Invasive Species ◽  
Survey Method ◽  
Specific Primers ◽  
Invasive Fishes ◽  
Specific Amplification ◽  
Species Specific ◽  
High Level

Peacock bass or the cichlids are known locally as top predator fishes which are invasive in Malaysia freshwater system. Detection probabilities for these fishes are typically low, especially using conventional capture-survey method due to the fish’s behaviour of hiding beneath the water’s surface. Hence, the environmental DNA (eDNA) monitoring is a relatively new approach that can be used to assess the distribution of these invasive fishes. Here, we report the strategy to develop small fragment (280- 400 bp) specific-specific primers for three selected invasive Cichla species namely, C. ocellaris, C. monoculus, and C. kelberi based on mitochondrial DNA (mtDNA) sequences. Current research showed that the developed species-specific primers from cytochrome oxidase I (COI) gene has high resolution at species level. Species-specific amplification tests also proved the specificity of the developed primers, securing the high- level species identification potential which may help in controlling the spread of alien invasive fish species.

Pamphilius japonicus feeds on Cornus: the first Old World record of Pamphiliidae (Hymenoptera) associated with Cornaceae

Zootaxa ◽  
2019 ◽  
Vol 4585 (2) ◽  
pp. 343
Author(s):  
AKIHIKO SHINOHARA ◽  
SHIN-ICHI IBUKI ◽  
TSUNEO KAKUDA ◽  
YUICHI KAMEDA
Keyword(s):  
Host Plant ◽  
First Record ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
World Record ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Old World ◽  
Cytochrome Oxidase Subunit 1 ◽  
Mitochondrial Cytochrome Oxidase

We identified newly discovered pamphiliid larvae feeding on Cornus in Tochigi and Ibaraki Prefectures, Honshu, Japan, with Pamphilius japonicus Shinohara, 1985, by molecular methods using mitochondrial cytochrome oxidase subunit 1 (COI) gene sequences. The host plant of P. japonicus was unknown. This is the first record of the Cornaceae as a host plant of the Pamphiliidae in the Old World. The larvae were solitary leaf-rollers on Cornus and discovered mainly on small young trees in shadowy forests. 

A new record of Ornithodoros (Pavlovskyella) verrucosus (Acari: Argasidae) from a porcupine burrow in western Iran

2019 ◽  
Vol 24 (5) ◽  
pp. 771
Author(s):  
Asadollah Hosseini Chegeni ◽  
Majid Tavakoli ◽  
Habibollah Koshki ◽  
Javad Khedri ◽  
Mohammad Hassan Kayedi
Keyword(s):  
Cytochrome Oxidase ◽  
18S Rdna ◽  
Genomic Dna ◽  
New Record ◽  
Morphological Characteristics ◽  
Species Group ◽  
Coi Gene ◽  
Cytochrome Oxidase Subunit ◽  
Western Iran ◽  
Blast Analysis

We found an Ornithodoros species with distinct morphological characteristics than Alveonasus genus and other O. (Pavlovskyella) species group. Tick specimens were collected from a porcupine burrow, located in Lorestan province, western Iran. Genomic DNA of tick was extracted using CTAB and then three gene fragments of cytochrome oxidase subunit I (COI) and 16S mtDNA & 18S rDNA were amplified by PCR. Tick specimens were identified as near to O. (P.) verrucosus and O. (P.) tholozani. So the identification was confirmed by BLAST analysis of 16S mtDNA & 18S rDNA and COI gene fragments. A taxonomic key to the native adult subfamily Ornithodorinae species of Iran are provided to facilitate their identification.

Revision and phylogeny of the subaptera-group of Phyllodromica (Blattoptera: Blattellidae: Ectobiinae), including a parthenogenetic species and the evaluation of COI sequences for species identification (DNA barcoding)

Zootaxa ◽  
2007 ◽  
Vol 1522 (1) ◽  
pp. 1-68 ◽  
Author(s):  
THOMAS KNEBELSBERGER ◽  
MICHAEL A. MILLER
Keyword(s):  
Species Identification ◽  
Dna Sequences ◽  
Sequence Data ◽  
Cladistic Analysis ◽  
Morphological Characteristics ◽  
Morphological Data ◽  
Coi Gene ◽  
Parthenogenetic Species ◽  
Mediterranean Countries ◽  
Coi Sequences

Until recently the subaptera-group of Phyllodromica contained only one species. The revision of the subaptera-group  herein consists of the two newly described bisexual species, P. iberica and P. quadracantha, endemic to the Iberian Peninsula and a parthenogenetic species, P. subaptera (Rambur, 1838), which is widely distributed over most of the Mediterranean countries and islands. Within P. iberica three conspecific morphotypes are distinguished. The morphological characteristics of the subaptera-group are described. The species and their distributions are described and depicted. A key for the morphological determination of P. quadracantha and the morphotypes of P. iberica is given. DNA sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene are included in the species descriptions. The sequence data are suitable for species identification (DNA barcodes). A cladistic analysis of the morphological data and a phylogenetic analysis of the DNA sequences were performed to infer the phylogenetic relationships between the species of the subaptera-group.

Identification of Neoceratitis asiatica (Becker) (Diptera: Tephritidae) based on morphological characteristics and DNA barcode

Zootaxa ◽  
2017 ◽  
Vol 4363 (4) ◽  
pp. 553
Author(s):  
SHAOKUN GUO ◽  
JIA HE ◽  
ZIHUA ZHAO ◽  
LIJUN LIU ◽  
LIYUAN GAO ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Trees ◽  
Gap Analysis ◽  
Dna Barcode ◽  
Morphological Characteristics ◽  
Coi Gene ◽  
Economic Losses ◽  
Morphological Identification ◽  
Lycium Barbarum ◽  
Accurate Identification

Neoceratitis asiatica (Becker), which especially infests wolfberry (Lycium barbarum L.), could cause serious economic losses every year in China, especially to organic wolfberry production. In some important wolfberry plantings, it is difficult and time-consuming to rear the larvae or pupae to adults for morphological identification. Molecular identification based on DNA barcode is a solution to the problem. In this study, 15 samples were collected from Ningxia, China. Among them, five adults were identified according to their morphological characteristics. The utility of mitochondrial DNA (mtDNA) cytochrome c oxidase I (COI) gene sequence as DNA barcode in distinguishing N. asiatica was evaluated by analysing Kimura 2-parameter distances and phylogenetic trees. There were significant differences between intra-specific and inter-specific genetic distances according to the barcoding gap analysis. The uncertain larval and pupal samples were within the same cluster as N. asiatica adults and formed sister cluster to N. cyanescens. A combination of morphological and molecular methods enabled accurate identification of N. asiatica. This is the first study using DNA barcode to identify N. asiatica and the obtained DNA sequences will be added to the DNA barcode database. 

Leptographium piriforme — first record for Europe and of potential pathogenicity

Biologia ◽  
2010 ◽  
Vol 65 (4) ◽  
Author(s):  
Robert Jankowiak ◽  
Miroslav Kolařík
Keyword(s):  
Pinus Sylvestris ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Morphological Characteristics ◽  
First Record ◽  
Nuclear Ribosomal Dna ◽  
Western Canada ◽  
First Report ◽  
Potential Pathogenicity ◽  
Ophiostomatoid Fungus

AbstractLeptographium piriforme Greif, Gibas & Currah, described in 2006 from western Canada has recently been collected in Jaroszowiec in Poland. The ophiostomatoid fungus L. piriforme was isolated from fallen Pinus sylvestris L. shoots infested by Tomicus spp. Isolates were identified to species using morphological characteristics and nuclear ribosomal DNA sequences. Two-year-old P. sylvestris seedlings were wound-inoculated with one isolate of L. piriforme. Leptographium piriforme produced necrotic lesions in the phloem of the seedlings, but none of the plant inoculated with this fungus died. This is the first report of L. piriforme from Poland and entire Europe.

A new moss salamander, genus Nototriton (Caudata: Plethodontidae), from the Cordillera de Talamanca, in the Costa Rica-Panama border region

Zootaxa ◽  
2018 ◽  
Vol 4369 (4) ◽  
pp. 487 ◽  
Author(s):  
ERICK ARIAS ◽  
BRIAN KUBICKI
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Dna Sequences ◽  
Morphological Characteristics ◽  
Mitochondrial Genes ◽  
Border Region ◽  
New Taxon ◽  
Cytochrome Oxidase Subunit ◽  
The Caribbean ◽  
Cordillera De Talamanca

A new salamander belonging to the genus Nototriton, subgenus Nototriton, is described from the Caribbean slopes of the southeastern Cordillera de Talamanca in Costa Rica, within Parque Internacional La Amistad, at an elevation ca. 1500 m a.s.l. This new taxon is distinguished from its congeners by its morphological characteristics and by its differentiation in DNA sequences of the 16S rRNA, cytochrome oxidase subunit I (COI), and cytochrome b mitochondrial genes. This new species represents the southernmost extension known for the genus Nototriton. 

scholarly journals Evidence of Microdochium Fungi Associated with Cereal Grains in Russia

2020 ◽  
Vol 8 (3) ◽  
pp. 340 ◽  
Author(s):  
Tatiana Yu. Gagkaeva ◽  
Aleksandra S. Orina ◽  
Olga P. Gavrilova ◽  
Nadezhda N. Gogina
Keyword(s):  
Western Siberia ◽  
Morphological Characteristics ◽  
First Record ◽  
Cereal Grains ◽  
Toxic Metabolites ◽  
Physiological Properties ◽  
Agar Media ◽  
Geographic Regions ◽  
Species Specific ◽  
Regions Of Russia

In total, 46 Microdochium strains from five different geographic regions of Russia were explored with respect to genetic diversity, morphology, and secondary metabolites. Based on the results of PCR, 59% and 28% of the strains were identified as M. nivale and M. majus, respectively. As a result of sequencing four genome regions, namely ITS, LSU, BTUB, and RPB2 (2778 bp), five genetically and phenotypically similar strains from Western Siberia were identified as M. seminicola, which, according to our findings, is the prevalent Microdochium species in this territory. This is the first record of M. seminicola in Russia. Attempts were made to distinguish between Microdochium species and to identify species-specific morphological characteristics in the anamorph and teleomorph stages and physiological properties. We examined the occurrence frequency of conidia with different numbers of septa in the strains of Microdochium. The predominance of three-septate macroconidia in M. majus was higher than that in M. nivale and typically exceeded 60% occurrence. Most M. majus and M. nivale strains formed walled protoperithecia on wheat stems. Only three strains of M. majus and one strain each of M. nivale and M. seminicola produced mature perithecia. The growth rate of M. seminicola strains was significantly lower on agar media at 5–25 °C than those of M. majus and M. nivale strains. Multimycotoxin analysis by HPLC-MS/MS revealed that the strains of three Microdochium species did not produce any toxic metabolites.

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