Collagenous Materials Enhance Healing of Chronic Skin Ulcers

AbstractType I collagen in a porous sponge form attracts fibroblasts in culture and accelerates repair of animal wounds. This study examines the effect of type I collagen sponge and flakes on healing of chronic skin ulcers. Patients included in this study had skin ulcers.Patients included in this study had skin ulcers characterized by loss of dermis and epidermis without exposure of muscle, tendon or bone. Patients showing evidence of systemic infection or patients with ulcers that decreased in area during an initial three week observation period were excluded.Three out of seven patients treated with a collagen sponge and twelve out of fourteen patients treated with collagen flakes showed a 40% decrease in wound area after six weeks of treatment. In comparison, eighteen control patients showed no change in wound area over the same time interval. These results suggest that collagen flakes are effective in initiating healing of chronic skin ulcers.

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Studies of Novel Hyaluronic Acid-collagen Sponge Materials Composed of Two Different Species of Type I Collagen

Journal of Biomaterials Applications ◽

10.1177/0885328206063502 ◽

2006 ◽

Vol 21 (3) ◽

pp. 265-281 ◽

Cited By ~ 19

Author(s):

Yung-Kai Lin ◽

Deng-Cheng Liu

Keyword(s):

Hyaluronic Acid ◽

Type I Collagen ◽

Collagen Sponge ◽

Type I

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Ointment ofBrassica oleraceavar.capitataMatures the Extracellular Matrix in Skin Wounds of Wistar Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/919342 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

Mariáurea Matias Sarandy ◽

Rômulo Dias Novaes ◽

Sérgio Luiz Pinto da Matta ◽

Jose Mario da Silveira Mezencio ◽

Marcelo Barreto da Silva ◽

...

Keyword(s):

Helianthus Annuus ◽

Type I Collagen ◽

Collagen Type I ◽

Mechanical Resistance ◽

Type I ◽

Collagen Types ◽

Wound Area ◽

Total Collagen ◽

Complex Process ◽

Made In

Wound healing is a complex process that aims to restore damaged tissue. Phytotherapeutics, such as cabbage,Brassica oleraceavar.capitata(Brassicaceae), and sunflower,Helianthus annuusL. (Asteraceae) oil, are used as wound healers. Five circular wounds, each 12 mm in diameter, were made in the dorsolateral region of each rat. The animals were divided into four groups: balsam (B. oleracea); ointment (B. oleracea); sunflower oil (Helianthus annuus); control (saline solution 0.9%). These products were applied daily for 20 days and every four days the tissues of different wounds were removed. The wound contraction area, total collagen, types I and III collagen, glycosaminoglycans, and tissue cellularity were analyzed. In the groups that received ointment and balsam there was reduction in the wound area on days 4, 8, 12, and 20. Throughout the trial period, the balsam and ointment groups showed a higher amount of total collagen, type I collagen, and glycosaminoglycan compared to the others groups. The rats in the groups treated withB. oleraceavar.capitatashowed a higher number of cells on days 8, 16, and 20.B. oleraceawas effective in stimulating the maturation of collagen and increasing the cellularity, as also in improving the mechanical resistance of the newly formed tissue.

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Mechanical Stimulation of Tissue Engineered Tendon Constructs: Effect of Scaffold Materials

Journal of Biomechanical Engineering ◽

10.1115/1.2800828 ◽

2007 ◽

Vol 129 (6) ◽

pp. 919-923 ◽

Cited By ~ 37

Author(s):

Victor S. Nirmalanandhan ◽

Matthew R. Dressler ◽

Jason T. Shearn ◽

Natalia Juncosa-Melvin ◽

Marepalli Rao ◽

...

Keyword(s):

Mechanical Stimulation ◽

Type I Collagen ◽

Collagen Gel ◽

Mechanical Stimulus ◽

Collagen Sponge ◽

The Other ◽

Type I ◽

Peak Strain ◽

Bovine Collagen

Our group has shown that numerous factors can influence how tissue engineered tendon constructs respond to in vitro mechanical stimulation. Although one study showed that stimulating mesenchymal stem cell (MSC)–collagen sponge constructs significantly increased construct linear stiffness and repair biomechanics, a second study showed no such effect when a collagen gel replaced the sponge. While these results suggest that scaffold material impacts the response of MSCs to mechanical stimulation, a well-designed intra-animal study was needed to directly compare the effects of type-I collagen gel versus type-I collagen sponge in regulating MSC response to a mechanical stimulus. Eight constructs from each cell line (n=8 cell lines) were created in specially designed silicone dishes. Four constructs were created by seeding MSCs on a type-I bovine collagen sponge, and the other four were formed by seeding MSCs in a purified bovine collagen gel. In each dish, two cell-sponge and two cell-gel constructs from each line were then mechanically stimulated once every 5min to a peak strain of 2.4%, for 8h∕day for 2 weeks. The other dish remained in an incubator without stimulation for 2 weeks. After 14 days, all constructs were failed to determine mechanical properties. Mechanical stimulation significantly improved the linear stiffness (0.048±0.009 versus 0.015±0.004; mean±SEM (standard error of the mean ) N/mm) and linear modulus (0.016±0.004 versus 0.005±0.001; mean±SEM MPa) of cell-sponge constructs. However, the same stimulus produced no such improvement in cell-gel construct properties. These results confirm that collagen sponge rather than collagen gel facilitates how cells respond to a mechanical stimulus and may be the scaffold of choice in mechanical stimulation studies to produce functional tissue engineered structures.

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Collagen fibrillogenesis in vitro: interaction of types I and V collagen

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142670 ◽

1986 ◽

Vol 44 ◽

pp. 210-211

Author(s):

Arthur J. Wasserman ◽

Kathy C. Kloos ◽

David E. Birk

Keyword(s):

Type I Collagen ◽

Corneal Stroma ◽

Minor Component ◽

Homogeneous Distribution ◽

Type I ◽

Type V Collagen ◽

Fibril Morphology ◽

Type V ◽

In Vitro Interaction

Type I collagen is the predominant collagen in the cornea with type V collagen being a quantitatively minor component. However, the content of type V collagen (10-20%) in the cornea is high when compared to other tissues containing predominantly type I collagen. The corneal stroma has a homogeneous distribution of these two collagens, however, immunochemical localization of type V collagen requires the disruption of type I collagen structure. This indicates that these collagens may be arranged as heterpolymeric fibrils. This arrangement may be responsible for the control of fibril diameter necessary for corneal transparency. The purpose of this work is to study the in vitro assembly of collagen type V and to determine whether the interactions of these collagens influence fibril morphology.

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