Effects on Hardness and Elastic Modulus for DSS-8 Peptide Treatment on Remineralization of Human Dental Tissues

ABSTRACTDental remineralization may be achieved by mediating the interactions between tooth surfaces with free ions and biomimetic peptides. We recently developed octuplet repeats of aspartate-serine-serine (DSS-8) peptide, which occurs in high abundance in naturally occurring proteins that are critical for tooth remineralization. In this paper, we evaluated the possible role of DSS-8 in enamel remineralization. Human enamel specimens were demineralized, exposed briefly to DSS-8 solution, and then exposed to concentrated ionic solutions that favor remineralization. Enamel nano-mechanical behaviors, hardness and elastic modulus, at various stages of treatment were determined by nanoindentation. The phase, microstructure and morphology of the resultant surfaces were characterized using the grazing incidence X-ray diffraction (GIXD), variable pressure scanning electron microscopy (VPSEM), and atomic force microscopy (AFM), respectively. Nanoindentation results show that the DSS-8 remineralization effectively improves the mechanical and elastic properties for demineralized enamel.

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Influence of DSS-8 on the remineralisation of Dentine

MRS Proceedings ◽

10.1557/proc-1187-kk05-34 ◽

2009 ◽

Vol 1187 ◽

Author(s):

Chia-Chan Hsu ◽

Elizabeth Marie Hagerman ◽

Hsiu-Ying Chung ◽

Wenyuan Shi ◽

Jenn-Ming Yang ◽

...

Keyword(s):

Elastic Modulus ◽

Grazing Incidence ◽

Variable Pressure ◽

X Ray Diffraction ◽

Tooth Remineralization ◽

Force Microscopy ◽

Atomic Force ◽

Human Dentin ◽

Tooth Surfaces

AbstractDental remineralization may be achieved by mediating the interactions between tooth surfaces with free ions and biomimetic peptides. We recently developed octuplet repeats of aspartate-serine-serine (DSS-8) peptide, which occurs in high abundance in naturally occurring proteins that are critical for tooth remineralization. In this paper, we evaluated the possible role of DSS-8 in dentin remineralization. Human dentin specimens were demineralized, exposed briefly to DSS-8 solution, and then exposed to concentrated ionic solutions that favor remineralization. Dentin nano-mechanical behaviors, hardness and elastic modulus, at various stages of treatment were determined by nanoindentation. The phase, microstructure and morphology of the resultant surfaces were characterized using grazing incidence X-ray diffraction, variable pressure scanning electron microscopy, and atomic force microscopy, respectively. Nanoindentation results show that DSS-8 remineralization effectively improves the mechanical and elastic properties of native dentin. Moreover, the hardness and elastic modulus for the DSS-8 treated dentin were significantly higher than surfaces remineralized without DSS-8.

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Structure and Properties of Murine and Human Dentin

MRS Proceedings ◽

10.1557/proc-874-l5.18 ◽

2005 ◽

Vol 874 ◽

Author(s):

Stefan Habelitz ◽

Shabnam Zartoshtimanesh ◽

Mehdi Balooch ◽

Sally J. Marshall ◽

Grayson W. Marshall ◽

...

Keyword(s):

Atomic Force Microscopy ◽

Elastic Modulus ◽

Dynamic Stiffness ◽

Model System ◽

Structure And Properties ◽

Force Microscopy ◽

Dental Tissues ◽

Atomic Force ◽

Main Structural Feature ◽

Human Dentin

AbstractMice are commonly considered the model mammal for many biomedical studies. In this work, mouse and human dentin were compared to specify structural and mechanical differences to establish a baseline for comparison of dental tissues between these species. Atomic force microscopy revealed tubules of about 1.0 to 1.6 μm in diameter as the main structural feature in dentin of both species. Nanoindentation yielded the elastic modulus about 15% lower in murine intertubular dentin while the hardness was almost equal. Dynamic stiffness mapping confirmed the lower elastic properties and also revealed that the peritubular region of increased mineralization around tubules is drastically reduced or maybe absent in murine dentin of this age. This study suggests that structural and mechanical differences need to be considered when murine dentin is used as a model system.

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Interface Structures and Electronic States of Epitaxial Tetraazanaphthacene on Single-Crystal Pentacene

Materials ◽

10.3390/ma14051088 ◽

2021 ◽

Vol 14 (5) ◽

pp. 1088

Author(s):

Yuki Gunjo ◽

Hajime Kamebuchi ◽

Ryohei Tsuruta ◽

Masaki Iwashita ◽

Kana Takahashi ◽

...

Keyword(s):

Single Crystal ◽

Photoelectron Spectroscopy ◽

Grazing Incidence ◽

Force Microscopy ◽

Interface Dipole ◽

Atomic Force ◽

Interfacial Structures ◽

Donor And Acceptor ◽

A Charge ◽

Interface Structures

The structural and electronic properties of interfaces composed of donor and acceptor molecules play important roles in the development of organic opto-electronic devices. Epitaxial growth of organic semiconductor molecules offers a possibility to control the interfacial structures and to explore precise properties at the intermolecular contacts. 5,6,11,12-tetraazanaphthacene (TANC) is an acceptor molecule with a molecular structure similar to that of pentacene, a representative donor material, and thus, good compatibility with pentacene is expected. In this study, the physicochemical properties of the molecular interface between TANC and pentacene single crystal (PnSC) substrates were analyzed by atomic force microscopy, grazing-incidence X-ray diffraction (GIXD), and photoelectron spectroscopy. GIXD revealed that TANC molecules assemble into epitaxial overlayers of the (010) oriented crystallites by aligning an axis where the side edges of the molecules face each other along the [1¯10] direction of the PnSC. No apparent interface dipole was found, and the energy level offset between the highest occupied molecular orbitals of TANC and the PnSC was determined to be 1.75 eV, which led to a charge transfer gap width of 0.7 eV at the interface.

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A Universal Model for the Log-Normal Distribution of Elasticity in Polymeric Gels and Its Relevance to Mechanical Signature of Biological Tissues

Biology ◽

10.3390/biology10010064 ◽

2021 ◽

Vol 10 (1) ◽

pp. 64

Author(s):

Arnaud Millet

Keyword(s):

Elastic Modulus ◽

Normal Distribution ◽

Biological Tissues ◽

Force Microscopy ◽

Polymeric Gels ◽

Atomic Force ◽

Clear Explanation ◽

Log Normal ◽

Log Normal Distribution

The mechanosensitivity of cells has recently been identified as a process that could greatly influence a cell’s fate. To understand the interaction between cells and their surrounding extracellular matrix, the characterization of the mechanical properties of natural polymeric gels is needed. Atomic force microscopy (AFM) is one of the leading tools used to characterize mechanically biological tissues. It appears that the elasticity (elastic modulus) values obtained by AFM presents a log-normal distribution. Despite its ubiquity, the log-normal distribution concerning the elastic modulus of biological tissues does not have a clear explanation. In this paper, we propose a physical mechanism based on the weak universality of critical exponents in the percolation process leading to gelation. Following this, we discuss the relevance of this model for mechanical signatures of biological tissues.

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Grazing incidence x-ray diffraction and atomic force microscopy investigations of germanium dots grown on silicon (001) by successive depositions of germanium through a thin silicon oxide layer

Applied Physics Letters ◽

10.1063/1.1715150 ◽

2004 ◽

Vol 84 (17) ◽

pp. 3295-3297 ◽

Cited By ~ 4

Author(s):

M. Derivaz ◽

P. Noé ◽

R. Dianoux ◽

A. Barski ◽

A. Coati ◽

...

Keyword(s):

Atomic Force Microscopy ◽

Oxide Layer ◽

Silicon Oxide ◽

Grazing Incidence ◽

X Ray Diffraction ◽

Silicon Oxide Layer ◽

X Ray ◽

Force Microscopy ◽

Atomic Force ◽

Thin Silicon

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The influence of carbon-encapsulated iron nanoparticles on elastic modulus of living human mesenchymal stem cells examined by atomic force microscopy

Micron ◽

10.1016/j.micron.2018.02.006 ◽

2018 ◽

Vol 108 ◽

pp. 41-48 ◽

Cited By ~ 15

Author(s):

Ida Dulinska-Molak ◽

Adrian Chlanda ◽

Jasmine Li ◽

Xinlong Wang ◽

Michal Bystrzejewski ◽

...

Keyword(s):

Stem Cells ◽

Atomic Force Microscopy ◽

Mesenchymal Stem Cells ◽

Elastic Modulus ◽

Iron Nanoparticles ◽

Human Mesenchymal Stem Cells ◽

Force Microscopy ◽

Atomic Force

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Preparation and Research on the Lateral Elastic Modulus of the MnO2 Nanowires

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.662.84 ◽

2013 ◽

Vol 662 ◽

pp. 84-87

Author(s):

Yong Jiang ◽

Jian Cheng Deng ◽

Yan Huai Ding ◽

Jiu Ren Yin ◽

Ping Zhang

Keyword(s):

Elastic Modulus ◽

Crystal Form ◽

Bending Test ◽

X Ray Diffraction ◽

Nanowire Diameter ◽

X Ray ◽

Three Point Bending ◽

Force Microscopy ◽

Atomic Force ◽

Mno2 Nanowires

MnO2 nanowires with large aspect ratio were successfully synthesized via a hydrothermal method. In this method, Mn(NO3)2 was as a source of manganese and NH4NO3 as an oxidant. The structure and morphology of the MnO2 nanowires were characterized by X ray diffraction (XRD) and scanning electron microscope (SEM). Their lateral elastic modulus was characterized via a nanoscale three-point bending test by atomic force microscopy (AFM) equipped with picoforce. The results indicate that the crystal form of MnO2 was β-MnO2. The elastic modulus of the nanowires decreased with the increase in nanowire diameter. This elastic modulus was in the range of 33.36-77.84GPa as the diameter ranged from 240 to 185nm.

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Silica Nanoparticles to Polish Tooth Surfaces for Caries Prevention

Journal of Dental Research ◽

10.1177/154405910808701007 ◽

2008 ◽

Vol 87 (10) ◽

pp. 980-983 ◽

Cited By ~ 30

Author(s):

R.M. Gaikwad ◽

I. Sokolov

Keyword(s):

Silica Nanoparticles ◽

Ex Vivo ◽

Bacterial Attachment ◽

Nanometer Scale ◽

Human Teeth ◽

Cariogenic Bacteria ◽

Force Microscopy ◽

Atomic Force ◽

Tooth Surfaces ◽

Scale Roughness

Although silica particles have been used for tooth polishing, polishing with nanosized particles has not been reported. Here we hypothesize that such polishing may protect tooth surfaces against the damage caused by cariogenic bacteria, because the bacteria can be easily removed from such polished surfaces. This was tested on human teeth ex vivo. The roughness of the polished surfaces was measured with atomic force microscopy (AFM). A considerably lower nanometer-scale roughness was obtained when silica nanoparticles were used to polish the tooth surfaces, as compared with conventional polishing pastes. Bacterial attachment to the dental surfaces was studied for Streptococcus mutans, the most abundant cariogenic bacteria. We demonstrated that it is easier to remove bacteria from areas polished with silica nanoparticles. The results demonstrate the advantage of using silica nanoparticles as abrasives for tooth polishing.

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