SiO2 Entrapment of Animal Cells for Hybrid Bioartificial Organs

ABSTRACTSi-alkoxides in gas phase are reactive towards the surface of animal cells, depositing a homogeneous layer of porous silica. This encapsulation method preserves cell viability and does not alter the hindrance of the biological load.In the prospective use for the design of a hybrid bioartificial liver, hepatocytes in a collagen matrix can be entrapped by the siliceous deposit which provides definite mechanical stability to the collagen matrix and molecular cutoff vs. high molecular weight proteins, including immunoglobulins. The functionality of the encapsulated cell load is maintained for the expressions of typical liver and pancreas metabolic activities.

Download Full-text

Associated Anisotropy of intrinsic NAD(P)H for monitoring changes in the metabolic activities of breast cancer cells in three-dimensional collagen matrix

Physical Chemistry Chemical Physics ◽

10.1039/d0cp06635d ◽

2021 ◽

Author(s):

Anh Cong ◽

Rafaela M. L. Pimenta ◽

Jon Holy ◽

Ahmed A Heikal

Keyword(s):

Breast Cancer ◽

Cell Culture ◽

Breast Cancer Cells ◽

Monolayer Culture ◽

Three Dimensional ◽

Collagen Matrix ◽

Living Cells ◽

Two Dimensional ◽

Metabolic Activities

The majority of in vitro studies of living cells are routinely conducted in a two-dimensional (2D) monolayer culture. Recent studies, however, suggest that 2D cell culture promotes specific types of...

Download Full-text

Nanoparticle prepared mechanically stable hierarchically porous silica granulates and their application as oxygen carrier supports for chemical looping combustion

Journal of Materials Chemistry A ◽

10.1039/c5ta01842k ◽

2015 ◽

Vol 3 (22) ◽

pp. 11863-11873 ◽

Cited By ~ 6

Author(s):

Yujing Liu ◽

Peter Kirchesch ◽

Thomas Graule ◽

Antje Liersch ◽

Frank Clemens

Keyword(s):

Mechanical Stability ◽

Oxygen Carrier ◽

Porous Silica ◽

Chemical Looping Combustion ◽

Chemical Looping ◽

Hierarchically Porous ◽

Templating Synthesis ◽

Hierarchical Nanomaterials

Viamodifying the templating synthesis of hierarchical nanomaterials, a strategy was proposed to generate bi-modal porous granulates with mechanical stability.

Download Full-text

Nucleic acid and protein synthesis and pattern regulation in hydra

Development ◽

10.1242/dev.21.1.55 ◽

1969 ◽

Vol 21 (1) ◽

pp. 55-70

Author(s):

S. G. Clarkson

Keyword(s):

Protein Synthesis ◽

Dna Synthesis ◽

Experimental Approach ◽

Direct Evidence ◽

Animal Cells ◽

Rna And Protein Synthesis ◽

Metabolic Activities ◽

Time Required ◽

Pattern Regulation

In a previous paper (Clarkson, 1969) data were presented which indicate that hypostome determination is accompanied by a large and rapid burst of RNA synthesis, a slight stimulation of protein synthesis, and no increase in DNA synthesis. More direct evidence concerning the relative importance of these metabolic activities in hypostome determination is reported in this paper. The experimental approach made use of the transplantation test of Webster & Wolpert (1966) in conjunction with some inhibitors of DNA, RNA and protein synthesis, the rationale being that if these metabolic activities play important roles in the determination of the hypostome, then their inhibition would be expected to have severe effects on the time required for this process. Regarding the inhibitors, hydroxyurea (HU) inhibits DNA synthesis in a variety of animal cells without altering rates of formation of RNA or protein (Young & Hodas, 1964; Yarbro, Kennedy & Barnum, 1965; Schwartz, Garofalo, Sternberg & Philips, 1965).

Download Full-text

Primary Stability of Revision Acetabular Reconstructions Using an Innovative Bone Graft Substitute: A Comparative Biomechanical Study on Cadaveric Pelvises

Materials ◽

10.3390/ma13194312 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4312

Author(s):

Federico Morosato ◽

Francesco Traina ◽

Ronja A. Schierjott ◽

Georg Hettich ◽

Thomas M. Grupp ◽

...

Keyword(s):

Calcium Phosphate ◽

Bone Graft ◽

Mechanical Stability ◽

Primary Stability ◽

Collagen Matrix ◽

Bone Graft Substitute ◽

Biomechanical Study ◽

Image Correlation ◽

Order Of Magnitude ◽

Acetabular Defects

Hip implant failure is mainly due to aseptic loosening of the cotyle and is typically accompanied by defects in the acetabular region. Revision surgery aims to repair such defects before implantation by means of reconstruction materials, whose morselized bone graft represents the gold standard. Due to the limited availability of bone tissue, synthetic substitutes are also used. The aim of this study was to evaluate if a synthetic fully resorbable tri-calcium phosphate-based substitute can provide adequate mechanical stability when employed to restore severe, contained defects, in comparison with morselized bone graft. Five cadaveric pelvises were adopted, one side was reconstructed with morselized bone graft and the other with the synthetic substitute, consisting of dense calcium phosphate granules within a collagen matrix. During the biomechanical test, cyclic load packages of increasing magnitude were applied to each specimen until failure. Bone/implant motions were measured through Digital Image Correlation and were expressed in terms of permanent and inducible translations and rotations. The reconstruction types exhibited a similar behavior, consisting of an initial settling trend followed by failure as bone fracture (i.e., no failure of the reconstruction material). When 2.2 Body Weight was applied, the permanent translations were not significantly different between the two reconstructions (p = 0.06–1.0) and were below 1.0 mm. Similarly, the inducible translations did not differ significantly (p = 0.06–1.0) and were below 0.160 mm. Rotations presented the same order of magnitude but were qualitatively different. Overall, the synthetic substitute provided adequate mechanical stability in comparison with morselized bone graft, thus representing a reliable alternative to treat severe, contained acetabular defects.

Download Full-text

Gas-Phase Deposition of Aminopropylalkoxysilanes on Porous Silica

Langmuir ◽

10.1021/la020869q ◽

2003 ◽

Vol 19 (8) ◽

pp. 3461-3471 ◽

Cited By ~ 40

Author(s):

Satu Ek ◽

Eero I. Iiskola ◽

Lauri Niinistö

Keyword(s):

Gas Phase ◽

Porous Silica ◽

Gas Phase Deposition

Download Full-text

The Effect of Thermal Treatment under Different Atmospheric Conditions on the Catalytic Performance of Nickel Supported on Porous Silica in the Gas-Phase Hydrogenation of Acetonitrile

Adsorption Science & Technology ◽

10.1260/026361707782398218 ◽

2007 ◽

Vol 25 (3-4) ◽

pp. 185-198 ◽

Cited By ~ 1

Author(s):

Pilar Braos-García ◽

Desireé Durán-Martín ◽

Antonia Infantes-Molina ◽

Dolores Eliche-Quesada ◽

Enrique Rodríguez-Castellón ◽

...

Keyword(s):

Thermal Treatment ◽

Gas Phase ◽

Porous Silica ◽

Catalytic Performance ◽

Atmospheric Conditions

Download Full-text

Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method

10.1117/12.279168 ◽

1997 ◽

Cited By ~ 11

Author(s):

Giovanni Carturan ◽

Giovanni Dellagiacoma ◽

M. Rossi ◽

R. Dal Monte ◽

M. Muraca

Keyword(s):

Animal Cells ◽

Bioartificial Organs

Download Full-text

Optiminization of regeneration at the stages of soft tissue augmentation using a collagen matrix

Эндодонтия Today ◽

10.36377/1683-2981-2021-19-4-317-319 ◽

2021 ◽

Vol 19 (4) ◽

pp. 317-319

Author(s):

A. B. Adzhieva ◽

I. A. Voronov ◽

S. S. Ivanov ◽

H. M. Nalchajyan

Keyword(s):

Soft Tissue ◽

Soft Tissues ◽

Mechanical Stability ◽

Three Dimensional ◽

Collagen Matrix ◽

Soft Tissue Augmentation ◽

Dimensional Structure ◽

Soft Tissue Regeneration ◽

Tissue Augmentation ◽

Cellular Environment

Lack of adequate width and thickness of periodontal or peri-implant soft tissues can compromise the aesthetics, function or survival of teeth and dental implants. Biomaterials are widely used in dentistry to overcome the disadvantages of autogenous tissue transplantation. The advantage of using biomaterials is that there is no need for re-surgery and that they are available in large quantities. The most widely used biomaterial for soft tissue augmentation is collagen, as it is believed to best mimic the natural cellular environment of the extracellular matrix, although other biomaterials are also candidates for soft tissue regeneration. Collagen matrices differ in composition, three-dimensional structure, elasticity and mechanical stability. Aim. is to review the literature on the optimization of regeneration at the stages of soft tissue augmentation using a collagen matrix.

Download Full-text