Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

Quantum Dots and FRET-Nanobeads for Probing Genes, Proteins, and Drug Targets in Single Cells

2003 ◽  
Author(s):  
Amit Agrawal ◽  
Xiaohu Gao ◽  
Nitin Nitin ◽  
Gang Bao ◽  
Shuming Nie
Keyword(s):  
Quantum Dots ◽  
Drug Targets ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Length Scale ◽  
Imaging And Spectroscopy

Quantum dots are tiny light-emitting particles on the length scale of 2–10 nm, and FRET-nanobeads for fluorophore-embedded nanoparticles on the length scale of 40–200 nm based on the phenomenon of fluorescence resonance energy transfer (FRET). These materials are emerging as a new class of biological labels with properties and applications that are not available with traditional organic dyes and fluorescent proteins. In this ASME contribution, we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We also show results from intracellular staining of actin filaments using FRET-nanobeads. These results raise new possibilities in disease diagnostics, drug and biochemical discovery, cancer imaging, molecular profiling, and disease staging.

Nonblinking Carbon Dots for Imaging and Tracking Receptors on Live Cell Membrane

2021 ◽  
Author(s):  
Qian Wang ◽  
Zhenzhen Feng ◽  
Hua He ◽  
Xiang Hu ◽  
Jian Mao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Membrane ◽  
Carbon Dots ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Semiconductor Quantum Dots ◽  
Live Cell

Blinking occurs with nearly all fluorophores including organic dyes, fluorescent proteins, semiconductor quantum dots and carbon dots (CDs). We developed non-blinking and photoresistant fluorescent CDs by introducing multiple aromatic domains...

Fluorescence Imaging of Stem Cells, Cancer Cells and Semi-Thin Sections of Tissues using Silica-Coated CdSe Quantum Dots

2011 ◽  
Vol 21 (4) ◽  
pp. 1365-1370 ◽  
Author(s):  
M. Vibin ◽  
R. Vinayakan ◽  
Annie John ◽  
V. Raji ◽  
C. S. Rejiya ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Cdse Quantum Dots ◽  
Thin Sections

Selective functionalization of II-VI semiconductor quantum dots with peptides and integrins of cancer cells for biophotonic applications

2007 ◽  
Author(s):  
Bakhysh Bairamov ◽  
Vladimir Toporov ◽  
Farid Bayramov ◽  
Vladislav Lanzov ◽  
Mitra Dutta ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Semiconductor Quantum Dots ◽  
Selective Functionalization

Multifunctional gadolinium-labeled silica-coated core/shell quantum dots for magnetic resonance and fluorescence imaging of cancer cells

RSC Advances ◽  
2014 ◽  
Vol 4 (40) ◽  
pp. 20641-20648 ◽  
Author(s):  
Babao Lin ◽  
Xiuzhong Yao ◽  
Yihua Zhu ◽  
Jianhua Shen ◽  
Xiaoling Yang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Magnetic Resonance ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
High Relaxivity ◽  
Core Shell Nanoparticles

Multifunctional gadolinium-labeled silica-coated core/shell nanoparticles with high relaxivity and photoluminescence were synthesized for dual-modal contrast.

Folic acid-conjugated core/shell ZnS:Mn/ZnS quantum dots as targeted probes for two photon fluorescence imaging of cancer cells

2011 ◽  
Vol 7 (3) ◽  
pp. 1327-1338 ◽  
Author(s):  
Malgorzata Geszke ◽  
Marek Murias ◽  
Lavinia Balan ◽  
Ghouti Medjahdi ◽  
Jaroslaw Korczynski ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Folic Acid ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Core Shell ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Recent Developments in the Use of Glyconanoparticles and Related Quantum Dots for the Detection of Lectins, Viruses, Bacteria and Cancer Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Pedro J. Hernando ◽  
Simone Dedola ◽  
María J. Marín ◽  
Robert A. Field
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Semiconductor Quantum Dots ◽  
The Past ◽  
Coated Nanoparticles ◽  
Gold Silver ◽  
Recent Developments ◽  
Protein Toxins ◽  
Silicon Based

Carbohydrate-coated nanoparticles—glyconanoparticles—are finding increased interest as tools in biomedicine. This compilation, mainly covering the past five years, comprises the use of gold, silver and ferrite (magnetic) nanoparticles, silicon-based and cadmium-based quantum dots. Applications in the detection of lectins/protein toxins, viruses and bacteria are covered, as well as advances in detection of cancer cells. The role of the carbohydrate moieties in stabilising nanoparticles and providing selectivity in bioassays is discussed, the issue of cytotoxicity encountered in some systems, especially semiconductor quantum dots, is also considered. Efforts to overcome the latter problem by using other types of nanoparticles, based on gold or silicon, are also presented.

scholarly journals Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors

Sensors ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 2909 ◽  
Author(s):  
Anirban Samanta ◽  
Igor L. Medintz
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Organic Dyes ◽  
Stokes Shift ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
Semiconductor Quantum Dots ◽  
Renilla Luciferase ◽  
Bioluminescent Protein

Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect: that the excited donor here is produced by biochemical means and not by an external illumination. Often the choice of BRET source is the bioluminescent protein Renilla luciferase, which catalyzes the oxidation of a substrate, typically coelenterazine, producing an oxidized product in its electronic excited state that, in turn, couples with a proximal fluorophore resulting in a fluorescence emission from the acceptor. The acceptors pertinent to this discussion are semiconductor quantum dots (QDs), which offer some unrivalled photophysical properties. Amongst other advantages, the QD’s large Stokes shift is particularly advantageous as it allows easy and accurate deconstruction of acceptor signal, which is difficult to attain using organic dyes or fluorescent proteins. QD-BRET systems are gaining popularity in non-invasive bioimaging and as probes for biosensing as they don’t require external optical illumination, which dramatically improves the signal-to-noise ratio by avoiding background auto-fluorescence. Despite the additional advantages such systems offer, there are challenges lying ahead that need to be addressed before they are utilized for translational types of research.

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