scholarly journals BIOCHEMICAL RESPONSES AND GENETIC EXPRESSIONS OF SYNTHETIC COMMON CARP POPULATIONS EXPOSED TO HIGH-AMMONIA REARING ENVIRONMENT

2021 ◽  
Vol 16 (1) ◽  
pp. 13
Author(s):  
Yogi Himawan ◽  
Alimuddin Alimuddin ◽  
Kukuh Nirmala ◽  
Imron Imron ◽  
Joni Haryadi
Keyword(s):  
Hsp70 Gene ◽  
Expression Ratio ◽  
Rearing Environment ◽  
Freshwater Aquaculture ◽  
Fish Breeding ◽  
Rearing Conditions ◽  
High Ammonia ◽  
Red Blood Cell Count ◽  
Resistant Group ◽  
Different Strains

Carp is one of the leading freshwater aquaculture commodities in Indonesia. Further improvement of carp strains by the Research Institute for Fish Breeding (RIFB), Sukamandi, Indonesia, has produced a synthetic F2 carp. The strain is assembled from different strains of carps and has shown better growth and health characteristics. Considering that high environmental ammonia (HEA) has affected most carp grow-out systems, this study aimed to determine the performance of the synthetic carp populations in a high ammonia rearing environment. The treatments were rearing media of the synthetic carp seed populations added with and without (control) 200 mg/L NH4Cl arranged in three replicates. A total of 30 fish seeds/aquarium, weighed 10-15 g/fish, was used in the study. Dissolved oxygen levels were maintained above 2 mg/L using aeration. This study shows that higher tolerant carp populations had red blood cells of 232.66 ± 17.24 cells/mL, indicating a direct effect of high ammonia on red blood cell count (p<0.05). Cortisol levels of 80.90 ± 6.35 ng/mL in resistant carp indicate significant differences (p<0.05). The relative expressions of the HSP70 gene in the liver (Log10) ranged between 0.72 and 2.80. The values demonstrate that high ammonia-resistant synthetic carp have a higher relative expression ratio of the HSP70 gene than the less resistant group. This research concluded that the populations of synthetic F2 carp showed a degree of resistance against high-ammonia rearing conditions. When it is ready for aquaculture, this synthetic carp strain could be farmed in high density using intensive systems in HEA-affected artificial lakes and reservoir

scholarly journals Extruded starter compound feeds for clarid catfish

2020 ◽  
Vol 2020 (2) ◽  
pp. 82-92
Author(s):  
Valentina Ivanovna Sidorova ◽  
Saule Zhangirovna Asylbekova ◽  
Nadezhda Ivanovna Yеnvareva ◽  
Saya Kashkinbayevna Koyshibaeva ◽  
Nina Sergeevna Badryzlova ◽  
...  
Keyword(s):  
Fish Species ◽  
Energy Exchange ◽  
Capital Investments ◽  
Fish Farms ◽  
Freshwater Aquaculture ◽  
Fish Breeding ◽  
Recycling Systems ◽  
Aquaculture Facilities ◽  
Aquaculture Development ◽  
Oxygen Equipment

Industrial fish-breeding is a promising trend for freshwater aquaculture development. The production of clarid catfish in Kazakhstan has advantages over traditionally grown fish species (trout, sturgeon) due to their valuable edible qualities. Clarid catfish grows very quickly even at high seeding densities, is undemanding to the environment, resistant to diseases, and effectively assimilates food. When growing clarid catfish in water recycling systems it is possible to refuse using oxygen equipment, because the fish species can use atmospheric air for breathing. As a result, the construction of such plants can save up to 40% of capital investments. In the course of the research, the physiological nutritional requirements of clarid catfish juveniles were established. Based on the data obtained there have been developed two extruded starter compound feed recipes for clarid catfish juveniles containing protein/fat 53.5 : 11.4 and 61.85 : 5.82 and energy exchange energy value varying within 16.46 - 17.12 MJ/kg. There have been conducted the production tests on assessing the efficiency of the developed compound feed and their intake by larvae and clarid catfish fry in the fish farms of “Kapshagayskoye NVH-1973”, LLP and “HalykBalyk”, LLP. The feed Aller Agua (Denmark) served as a control feed for comparing the nutritional and consumer qualities of the developed feed for trout. The determined feeding ratio of the developed feed did not exceed 0.93 units, it was insignificantly inferior to the import feed. The fry survival rate made 76% when using both the control and developed feed. The compound feeds for clarid catfish juveniles were developed by extruding, which allowed to obtain increased digestibility, water resistance and extended storage life. Introducing these feeds into the fish breeding practice will help to increase the efficiency of clarid catfish fry growth and expand opportunities of aquaculture facilities.

Survival ability of Mexican fruit fly males from different strains in presence of the predatory orb-weaving spider Argiope argentata (Araneae: Araneidae)

2018 ◽  
Vol 109 (3) ◽  
pp. 279-286
Author(s):  
A. Dor ◽  
P. Liedo
Keyword(s):  
Integrated Management ◽  
Management Strategies ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Colony Management ◽  
Rearing Conditions ◽  
Negative Effect ◽  
Different Strains

AbstractThe sterile insect technique (SIT) is a key element for the integrated management of pest populations of the Mexican fruit fly, Anastrepha ludens, in Mexico. Its success depends on the survival of mass-reared sterile males and their ability to mate with wild females. However, colonization and mass-rearing conditions can adversely affect their ability to avoid predators. To test if colony management strategies could contribute to improve field survival abilities of mass-reared flies, we compared the survival of males exposed to the orb-weaver spider Argiope argentata. Males compared originated from three strains with different colonization strategies: (a) a colony started from field-collected wild flies (replacement), (b) a colony started by hybridizing wild males with mass-reared adapted females (hybrid) and (c) a colony started with mass-reared males selected on the basis of their survival ability and mating competitiveness in field cages (selected). Mass-reared males and wild males were used as controls. Males were exposed to spiders under laboratory cage conditions. Overall, wild males showed better survival ability than mass-reared males. Regarding the colonization approach, wild males survived better than a hybrid, replaced and selected males. We conclude that mass-rearing conditions have a strong negative effect on the ability of males to escape spiders. The colonization systems evaluated did not counter this effect. The lower survival of males from the selected colony suggests that the selection over one generation did not contribute to improve males’ predator avoidance and escape abilities and probably needs to be modified. Possible explanations for this and implications on colonization and colony management for SIT purpose are discussed.

scholarly journals Study on the proposal of a community of freshwater aquaculture and vegetable farming in An Giang

2020 ◽  
Vol 4 (1) ◽  
pp. First
Author(s):  
Thu Hong Anh Nguyen ◽  
Tran Thi Hieu ◽  
Tra Van Tung ◽  
Nguyen Viet Thang ◽  
Huyen Khon Nguyen ◽  
...  
Keyword(s):  
Solid Waste ◽  
Traditional Farming ◽  
Nitrogen And Phosphorus ◽  
Reuse Water ◽  
Freshwater Aquaculture ◽  
Standard Index ◽  
Fish Breeding ◽  
Vegetable Farming ◽  
Additional Income

The objective of this study is to propose models of livelihoods symbiosis between freshwater aquaculture operations and vegetable farming. This model functioned as the way to reuse water and nutrients discharged from aquaculture activities, which caters for vegetable farming. The efficiency of the model is to minimize a large amount of solid waste disregarded directly to the receiving source, which was 315.098 kg. The quality of wastewater had been improved after releasing to the source. Total amount of carbon (TOC), nitrogen and phosphorus was 7.56%, 8.27% and 0.64% respectively, all of which meet the standard index of waste disragrded directly into the environment. In addition, the model also brings the additional income for households with 24.9 million VN dong after every fish breeding in comparison with traditional farming model.

scholarly journals NEW ACCLIMATIZATION OBJECTS IN FRESHWATER AQUACULTURE: GAMETOGENESIS, SEXUAL CYCLES, FISH-BREEDING DEVELOPMENT

Fisheries ◽  
2020 ◽  
Vol 2020 (3) ◽  
pp. 83-88
Author(s):  
Alexey Bagrov ◽  
Vera Ilyasova ◽  
Evgeniy Melchenkov ◽  
Vera Kalmykova
Keyword(s):  
Freshwater Fish ◽  
Freshwater Aquaculture ◽  
Fish Breeding ◽  
Artificial Breeding

A perennial studies of gametogenesis and sexual cycles during acclimatization and artificial breeding of nine freshwater fish aquacultural species. General relations of gametogenesis and sexual cycles are revealed, the species peculiarities of gonads formation and maturation speed are determined. A scheme of artificial breeding optimization is proposed.

scholarly journals ANALISIS KERAGAMAN GENETIK TIGA STRAIN NILA MERAH (Oreochromis sp) DENGAN ANOVA RAPD

2017 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Iskandariah Iskandariah ◽  
Otong Zenal Arifin ◽  
Rudhy Gustiano
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Nile Tilapia ◽  
Genetic Variance ◽  
Laboratory Research ◽  
Red Tilapia ◽  
Freshwater Aquaculture ◽  
Genetic Strain ◽  
Different Strains ◽  
Random Amplified Polymorphism Dna

Analysis Genetic Variation of Three Strains of Red Tilapia by Anova of RAPD.          Study on the genetic variance of three strains of red tilapia (Oreochromis sp) had been conducted in the Biology Molecular Laboratory, Research Institute for Freshwater Aquaculture (RIFA) Bogor.  Three different strains, Red NIFI from Thailand,  Red Tilapia from Lido lake and Red Tilapia from Bogor’s farmer were analyzed in the study.  Observation used Random Amplified Polymorphism DNA (RAPD) with OPA-03, OPA-04, OPC 14 and OPC-15 primers.  The results showed that only OPA-03 primer was able to amplify numerous samples.  Further analysis showed that the percentage of polimorphic range was between 16.67 – 38.89%, heterozygosity value 0.0378 – 0.1536 and genetic distance among strain 0.3051 – 0.6037.Keywords : RAPD, genetic, strain, nile tilapia, oreochromis ABSTRAK           Penelitian mengenai variasi genetik tiga strain nila merah dari 3 lokasi yang berbeda telah dilakukan di Laboratorium Molekuler Biologi, Balai Riset Perikanan Budidaya Air Tawar (BRPBAT) Bogor.  Strain yang diamati meliputi jenis nila Red NIFI dari Thailand, nila merah dari Danau Lido dan nila merah dari Petani Bogor. Penelitian menggunakan metode analisis Random Amplified Polymorphism DNA (RAPD), dengan menggunakan primer OPA-03, OPA-04, OPC-14 dan OPC-15.  Hasil pengamatan menunjukkan hanya OPA-03 yang dapat menghasilkan amplifikasi dalam jumlah sampel yang memadai.  Hasil analisis menunjukkan bahwa persentase polimorfik berkisar antara 20.00-40.00%, dengan nilai heterozigositas 0.0604–0.1516 dan jarak genetik antar strain 0.1770-0.4865.  Kata kunci : RAPD, genetik, strain, ikan nila, oreochromis

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

Rearing Conditions and Ethanol Consumption by Rats

1973 ◽  
Vol 34 (2) ◽  
pp. 520-524 ◽  
Author(s):  
Kathy Kazmaier ◽  
Richard E. Butcher ◽  
R. J. Senter ◽  
Robert M. Stutz
Keyword(s):  
Ethanol Consumption ◽  
Rearing Conditions

Results of Surveillance Studies of Infectious Fish Diseases in Freshwater Aquaculture of Ukraine

2015 ◽  
Vol 2 (2) ◽  
pp. 32-38 ◽  
Author(s):  
N. Matvienko ◽  
A. Vashchenko ◽  
I. Tsiganok ◽  
L. Buchatsky
Keyword(s):  
Rainbow Trout ◽  
Infectious Diseases ◽  
Cell Cultures ◽  
Standardized Test ◽  
Age Groups ◽  
Infectious Pancreatic Necrosis Virus ◽  
Test System ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Freshwater Aquaculture ◽  
Pancreatic Necrosis Virus

Aim. To investigate the epizootic state of fi sheries in Ukraine; to study the biological specifi cities of viral and bacterial isolates of fi sh in freshwater aquaculture. Methods. The epizootic state of fi sheries was defi ned ac- cording to the surveillance plan for fi sheries, virological (biosampling of sensitive fi sh species, virus isolation on sensitive passaged cell cultures), serological (enzyme immunoassay, virus neutralization test using sensitive passaged cell cultures) and molecular-biological (reverse transcriptase polymerase chain reaction − RTPCR) methods of investigation were used. The pathogenicity of the isolated bacteria was studied in the biosample. The identifi cation was performed using Bergey’s Manual. The express-identifi cation of bacteria was performed using the standardized test-system API 20E Bio Merieux (France). Results. The IPNV isolates of rainbow trout were fi rst isolated in the fi sheries of different forms of ownership in the western regions of Ukraine (Volyn, L’viv, Transcarpathian, Chernivtsi regions). It was demonstrated that different age groups of carp are infested with the virus in the fi sheries of L’viv, Donetsk, Chernihiv, Kyiv, and Odesa regions which testifi es to a wide spread of the virus in Ukraine. Out of fi sh infectious diseases the red spot-like disease and the swim bladder infl ammation of carp, the diseases of young trout and sturgeon were detected in the investigated fi sheries of Ukraine. Conclusions. The epizootic data were used to estimate the condition of the fi sheries in Ukraine in terms of fi sh infectious diseases. An infectious pancreatic necrosis virus, new for Ukraine, was revealed. It was found to affect rainbow trout (Oncorhynchus mykiss , Walbaum, 1792) and the spread of SVCV in carp fi sheries was demonstrated. As for bacterial fi sh diseases, the decrease in the epizootic situation was described along with considerable extension of the range of species of bacterial pathogens of fi sh. Annual systematic monitoring and measures of preventing the introduction of the agents of infectious diseases are the guarantee of protection of the specialized fi sheries of Ukraine.

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