scholarly journals GENETIC, COLORATION, AND GROWTH PERFORMANCE OF TWO DIFFERENT VARIETIES OF Kappaphycus alvarezii

2007 ◽  
Vol 2 (1) ◽  
pp. 23
Author(s):  
Sulaeman Sulaeman ◽  
Andi Parenrengi ◽  
Emma Suryati ◽  
Rosmiati Rosmiati
Keyword(s):  
Growth Performance ◽  
Dna Analysis ◽  
Kappaphycus Alvarezii ◽  
Universal Primers ◽  
Seaweed Farming ◽  
South Sulawesi ◽  
Coloration Pattern ◽  
Coloration Patterns ◽  
Genetic Assessment ◽  
Rapd Fragment

Two different colors (green and brown) of Kappaphycus alvarezii have been farmed in Indonesian waters for many years. This study aimed at comparing two ‘varieties’, i.e. green and brown, both genetically and morphologically. Samples for DNA analysis were collected from a farmer in Pinrang Regency, South Sulawesi. Five universal primers i.e. Ca-01, Ca-02, P-40, P-50, and DALRP were selected to obtain DNA genetic markers in differentiating the green and brown varieties. To compare coloration patterns during cultivation and the growth performance of both varieties, a field experiment was performed in a seaweed farming area in Pinrang Regency, during dry season of August-September 2004. The result of genetic assessment showed that the five selected primers revealed different RAPD banding pattern for both varieties. P-50 and DALRP primers demonstrated the greatest amplification in differentiating RAPD fragment between green and brown varieties. Fragment 900 bp and 1.300 bp were consistently generated in the green variety but were not amplified in the brown variety. The result of the field study confirmed that the coloration pattern of green and brown varieties was fixed; no interchange in color occurred during one crop cultivation.

scholarly journals Epiphyte Identification on Kappaphycus alvarezii Seaweed Farming Area in Arungkeke Waters, Jeneponto and The Effect on Carrageenan Quality

2019 ◽  
Vol 24 (3) ◽  
pp. 146 ◽  
Author(s):  
Sri Redjeki Hesti Mulyaningrum ◽  
Hidayat Suryanto Suwoyo ◽  
Mudian Paena ◽  
Bunga Rante Tampangallo
Keyword(s):  
Kappaphycus Alvarezii ◽  
Morphological Characteristics ◽  
Gel Strength ◽  
Test Analysis ◽  
Seaweed Farming ◽  
South Sulawesi ◽  
Farming Area ◽  
Sample Data ◽  
Carrageenan Yield ◽  
Carrageenan Quality

Kappaphycus alvarezii which is widely cultivated at sea is susceptible to other algae that drift away and attach as epiphyte. This study aims to identify epiphyte on seaweed farming K. alvarezii and its effect on carrageenan quality. The study was conducted on K. alvarezii seaweed farming area in Jeneponto, South Sulawesi. Sample of epiphyte was obtained from four stations. Epiphyte was identified in laboratory based on its morphological characteristics and calculated its density. Carrageenan yield and gel strength of healthy seaweed and those infected one were analyzed in laboratory in triplicates for each sample. Data were statistically analyzed using independent samples t-test analysis. Present study showed that there were 6 epiphytes species on the seaweed farming area, namely: Entheromorpha intestinalis, Ceramium sp., Neosiphonia apiculata, Chaetomorpha crassa, Hypnea sp., and Gracilaria sp. The average of epiphytic density in cultivation area was (24.26±9.64)%. Healthy seaweed and infected one had significantly different carrageenan yield and gel strength (P<0.05). Healthy seaweed had higher carrageenan yield (48.17±1.62)% and gel strength (1130.76±8.42) g cm-² than infected seaweed which had carrageenan yield (42.47±0.23)% and gel strength (958.22±10.85) g cm-².

Differential coloration patterns in the intertidal crab Neohelice (=Chasmagnathus) granulata

2010 ◽  
Vol 91 (5) ◽  
pp. 1041-1047 ◽  
Author(s):  
Agustina Méndez Casariego ◽  
Tomás Luppi ◽  
Oscar Iribarne
Keyword(s):  
Salt Marshes ◽  
Field Experiments ◽  
Spatial Segregation ◽  
Body Coloration ◽  
Body Regions ◽  
Intertidal Crab ◽  
Coloration Pattern ◽  
Chasmagnathus Granulata ◽  
Colour Changes ◽  
Coloration Patterns

Many crustaceans with spatial segregation may present differential body coloration depending on the habitat they inhabit. Since crustaceans are unable to synthesize carotenoids, individuals must acquire them from their diet, so diet changes can modify individual coloration. The burrowing crab Neohelice (=Chasmagnathus) granulata inhabits the intertidal, from the uppermost parts of salt marshes to the lowest mudflat zones with a spatial segregation of different size-classes. They are primarily deposit feeders in mud flats and herbivorous–detritivorous in the salt marsh. We analysed the effect of intermoult length on the carapace colour of N. granulata, and the changes in carapace colour with crab size and zone. Crab colour varied between unvegetated and vegetated areas. Some of these differences were in part caused by colour changes related to crab size. Larger crabs show less intensive colours, and given that they inhabit the upper part of the intertidal a differential coloration pattern occurred between zones. In field experiments intermoult duration affected crab colour and some body regions analysed showed differences between zones unrelated to size.

scholarly journals FAKTOR PENGELOLAAN YANG MEMPENGARUHI TINGKAT PRODUKSI RUMPUT LAUT Kappaphycus alvarezii DI PERAIRAN PANTAI SELATAN PROVINSI SULAWESI SELATAN

2016 ◽  
Vol 5 (3) ◽  
pp. 491
Author(s):  
Erna Ratnawati ◽  
Akhmad Mustafa ◽  
Rohama Daud
Keyword(s):  
Coastal Waters ◽  
Management Practices ◽  
Kappaphycus Alvarezii ◽  
The South ◽  
Dummy Variable ◽  
South Sulawesi ◽  
Yield Production ◽  
Long Line ◽  
Seed Origin ◽  
Seaweed Production

Perairan pantai Kabupaten Jeneponto, Bantaeng, dan Bulukumba merupakan sentra produksi rumput laut Kappaphycus alvarezii di Sulawesi Selatan. Pengelolaan budidaya yang dilakukan oleh pembudidaya di daerah tersebut cukup bervariasi sehingga dilakukan penelitian yang bertujuan untuk mengetahui faktor pengelolaan budidaya yang mempengaruhi produksi rumput laut. Metode survai melalui pengajuan kuesioner kepada 62 responden secara terstruktur. Sebagai peubah tidak bebas dalam penelitian ini adalah produksi rumput laut, sedangkan peubah bebas adalah faktor pengelolaan budidaya. Analisis regresi berganda dengan peubah boneka digunakan untuk memprediksi produksi rumput laut. Hasil penelitian menunjukkan bahwa produksi rumput laut di perairan selatan Sulawesi Selatan berkisar antara 463-5.000 dengan rata-rata 1.502,3 kg kering/3.000 m2 yang dibudidayakan dengan tali panjang. Faktor pengelolaan budidaya yang mempengaruhi produksi rumput laut adalah jarak antar tali ris, jarak antar rumpun dalam tali ris, hama baronang, penyakit ice-ice, bobot bibit, asal bibit dan sumber cemaran. Untuk meningkatkan produksi rumput laut di perairan selatan Sulawesi Selatan dapat dilakukan melalui peningkatan bobot bibit antara 36,9 sampai 100,0 g/rumpun, menggunakan bibit yang tidak diangkut terlalu lama, tidak menambah jarak antar tali ris sampai melebihi 1,0 m, tidak menambah jarak antar rumpun dalam tali ris yang melebihi 25 cm serta melakukan penanaman berdasar kalender musim tanam untuk mencegah terjadinya serangan hama dan penyakit serta cemaran.Coastal waters of Jeneponto, Bantaeng, and Bulukumba Regencies are the centre of seaweed Kappaphycus alvarezii production in South Sulawesi. Culture management practices applied by farmers in these areas are highly variable. Therefore, this research was conducted to study culture management practices affect the seaweed production in the area. Field survey was conducted by interviewing 62 respondents using questionnaires. The dependent variable in this research was seaweed production, while the independent variables were culture management factors. Multiple regressions with dummy variable were employed to analyze the data to predict seaweed production. The results show that the seaweed productions per one cycle in the south coastal waters of South Sulawesi were ranging from 463 to 5,000 kg dry/3,000 m2. Using long line method, the average yield production was 1,502.3 kg dry/3,000 m2. Culture management practices that affect the seaweed production were distance between ropes, distance between seaweed seeds along the rope, rabbitfish predatory, ice-ice disease, weight of seed, seed origin, and source of pollution. Increasing seaweed production in the south coastal waters of South Sulawesi could be done through increasing weight of seed from 36.9 to 100.0 g/clump, quicker transport of seed, maintaining the distance between the ropes not more than 1.0 m, not increasing the distance between clump along the ropes more than 25 cm along as well as conducting seed planting based on cultivating season to prevent pest diseases and pollution.

scholarly journals KARAKTERISTIK DAN KESESUAIAN PERAIRAN UNTUK BUDIDAYA RUMPUT LAUT DI KABUPATEN KEPULAUAN SANGIHE, SULAWESI UTARA

2017 ◽  
Vol 12 (2) ◽  
pp. 187
Author(s):  
Andi Akhmad Mustafa ◽  
Tarunamulia Tarunamulia ◽  
Hasnawi Hasnawi ◽  
I Nyoman Radiarta
Keyword(s):  
Water Quality ◽  
Carrying Capacity ◽  
Kappaphycus Alvarezii ◽  
Coastal Areas ◽  
Quality Data ◽  
Water Quality Data ◽  
Seaweed Farming ◽  
Long Line ◽  
Surrounding Areas ◽  
Seaweed Culture

Kabupaten Kepulauan Sangihe dengantiga pulau terdepannya memiliki potensi untuk pengembangan budidaya rumput laut (Kappaphycus alvarezii), namun belum tersedia data kondisi perairannya.  Kajian bertujuan untuk mengetahui karakteristik, kesesuaian, dan daya dukung perairan untuk budidaya rumput laut di kawasan pesisir Kabupaten Kepulauan Sangihe.  Data  kondisi perairan yang dikumpulkan berupa lingkungan fisik dan kualitas air.  Analisis dengan weighted linear combinationdalam SIG dilakukan untuk penentuan kesesuaian perairan danbesarnya kapasitas perairan digunakan untuk penentuan daya dukung perairan. Hasil kajian menunjukkan bahwa, karakteristik perairan Kabupaten Kepulauan Sangihe dapat mendukung usaha budidaya rumput laut, namun kedalaman perairan yang relatif dangkal dan adanya alur pelayaran yang menjadi faktor pembatas dalam kesesuaian lahan untuk budidaya rumput laut. Dari 4.839,36 ha kawasan pesisir yang dikaji di Teluk Talengen (Kecamatan Tabukan Tengah), Teluk Manalu (Kecamatan Tabukan Selatan), dan Teluk Dagho dan sekitarnya (Kecamatan Tamako dan Manganitu Selatan) dijumpai kawasan pesisir seluas 181,80 ha yang tergolong sangat sesuai; 852,82 ha yang tergolong cukup sesuai; 3.633,75 ha yang tergolong kurang sesuai; dan 179,99 ha yang tergolong tidak sesuai untuk budidaya rumput laut metoda tali panjang.  Budidaya rumput laut metoda tali panjang di Kabupaten Kepulauan Sangihe dapat dilakukan di Teluk Talengen, Teluk Manalu, dan Teluk Dagho dan sekitarnya  dengan pengembangan kawasan maksimal masing-masing seluas 324; 559; dan 1.171 ha yang dapat digunakan untuk masing-masing 1.296, 2.236, dan 4.684 unit rakit budidaya rumput laut berukuran 50 x 50 m.  Sangihe Archipelago Regency with its three outlying islands has the potential for seaweed farming development (Kappaphycus alvarezii), regrettably reliable water quality data are scarcely available in this region. The study was aimed to determine the characteristics, suitability, and carrying capacity of waters for seaweed farming in the coastal areas of Sangihe Archipelago Regency. The observed environmental quality of coastal waters included physical environment and water quality. A weighted linear combination  in a GIS environment method was applied to determine the suitability of waters and the capacity of coastal water to accommodate the maximum surface area of the farm was used to determine the carrying capacity of waters. The results of the study indicated that the characteristics of waters in Sangihe Archipelago Regency provide suitable environment for seaweed culture, however the relatively shallow waters and the existence of the shipping lanes in the study region can become major limiting factors for seaweed culture and development. Of 4,839.36 hectares of the coastal areas studied in Talengen Bay (Tabukan Tengah Subdistrict), Manalu Bay (South Tabukan Subdistrict), and Dagho Bay and surrounding areas (Tamako and Manganitu Selatan Subdistricts), a total of 181.80 ha were classified as very suitable; 852.82 ha were moderately suitable; 3,633.75 ha were less suitable; and 179.99 ha were not suitable for seaweed long-line culture method. Further analysis showed that seaweed culture of long-line method can be effectively practiced in Talengen Bay, Manalu Bay, and Dagho Bay and surrounding areas with the maximum development areas of 324, 559, and 1,171 ha respectively of which can be used for allocating 1,296, 2,236, and 4,684 culture raft units respectively, with the size of 50 x 50 m per unit.

scholarly journals Different Cultivation Periods and Seedling Sources on the Growth of Kappaphycus alvarezii Cultivated in Pangkep Waters, South Sulawesi, Indonesia

2017 ◽  
Vol 18 (1) ◽  
pp. 33
Author(s):  
Luqman Saleh ◽  
Muslimin Muslimin ◽  
Muhammad Ikbal Illijas ◽  
Ardiansyah Ardiansyah
Keyword(s):  
Kappaphycus Alvarezii ◽  
South Sulawesi

scholarly journals Botanical Origin of the Brazilian Red Propolis: a New Approach Using DNA analysis

2014 ◽  
Vol 58 (2) ◽  
pp. 79-85 ◽  
Author(s):  
Sona Jain ◽  
Giulia Marchioro ◽  
Lucyana Mendonça ◽  
Marcus Batista ◽  
Edilson Araujo
Keyword(s):  
Dna Sequencing ◽  
Dna Analysis ◽  
Chemical Constituents ◽  
Universal Primers ◽  
Chemical Compositions ◽  
Botanical Origin ◽  
Chemical Marker ◽  
New Approach ◽  
Geographical Regions ◽  
Fast Liquid Chromatography

Abstract Propolis is produced by the honeybees by using resin and other plant secretions. Propolis from different geographical regions have different chemical compositions. This is because the chemical constituents of propolis depend on the vegetation surrounding the apiary. In this report we present a new approach using DNA barcoding for the identification of the botanical origin of propolis. Red propolis samples were collected at different times of the year from the state of Sergipe situated in Northeast Brazil. Extraction of the DNA from propolis was made using a CTA B method. Amplification was done using ITS 2 universal primers, followed by DNA sequencing. Sequence analysis confirmed the presence of Dalbergia ecastaphyllum in the Brazilian red propolis. Formononetin is a chemical marker for the Brazillian red propolis and D. ecastaphyllum. Propolis samples analysed by DNA sequencing, were also checked by Ultra-Fast Liquid Chromatography for the presence of formononetin. Peaks corresponding to formononetin were observed in all the analysed propolis samples. This is the first report of the botanical origin of propolis using DNA technology.

scholarly journals Species detections in environmental DNA using metabarcoding: a useful tool in estuaries monitoring

2015 ◽  
Author(s):  
Adrián Mártinez-Marqués ◽  
Carlos Enrique Carleos ◽  
Eva García-Vazquez ◽  
Yaisel J. Borrell Pichs
Keyword(s):  
Dna Analysis ◽  
Anthropogenic Activities ◽  
Dna Barcode ◽  
Environmental Dna ◽  
Coi Gene ◽  
Universal Primers ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Cantabrian Coast ◽  
Taxonomic Groups

Estuaries are amongst the most productive habitats in Earth, producing more organic materia than forests, meadows or agricultural lands. In addition, estuaries exhibit high, and precious, biodiversity levels. In this study an environmental DNA analysis of the two most important estuaries in Asturias (Cantabrian Coast, north Iberia) in terms of food production (Ría del Eo and Ría de Villaviciosa) was carried out. The objective was to monitor aquatic biodiversity and also to detect alien species that can be associated with anthropogenic activities (e.g.: aquaculture). To achieve these objectives, a metabarcoding methodology based in NGS (next generation sequencing) and the mitochondrial COI gene as a DNA Barcode was used. Results showed that this methodology was useful to detect the presence of three different non-native genera (Crepidula, Lymnaea, Macrobrachium) that are probably parasitating species cultured in these estuaries. It is true that Metabarcoding has still unsolved problems such as the lack of 100% universal primers and paucity of referenced sequences for some taxonomic groups in the databases. However, it represents already a powerful tool to manage the resources of these important ecosystems and to guarantee their long-term sustainailibity.

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