scholarly journals DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER

2012 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Huria Marnis ◽  
Raden Roro Sri Pudji Sinarni Dewi ◽  
Imron Imron ◽  
Bambang Iswanto
Keyword(s):  
Growth Hormone ◽  
Clarias Gariepinus ◽  
Stomach Muscle ◽  
African Catfish ◽  
Growth Hormone Gene ◽  
Caudal Fin ◽  
Expression Levels ◽  
Striped Catfish ◽  
Pcr Method ◽  
High Level

Faster growing African catfish can be produced by transgenesis. This study was conducted to investigate the distribution and expression of growth hormone gene (PhGH) in various organs of the transgenic African catfish (Clarias gariepinus) founder (F0). Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, caudal fin, gill and eye. Transgene expression levels were analyzed using the method of reverse transcriptase-polymerase chain reaction (RT-PCR), -actin gene used as internal controls. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish founder. The high level of PhGH expression was found in the liver, pituitary, intestine and brain; smaller amounts were detectable in muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75 PhGH/-actin mRNA. The expression levels of PhGH had positive correlation with tissue and body size (P<0.05).

scholarly journals DETECTION, TRANSMISSION, AND EXPRESSION cDNA GROWTH HORMONE GENE (PhGH) OF STRIPPED CATFISH IN F-1 TRANSGENIC AFRICAN CATFISH

2014 ◽  
Vol 9 (2) ◽  
pp. 89
Author(s):  
Huria Marnis ◽  
Bambang Iswanto ◽  
Rommy Suprapto ◽  
Imron Imron ◽  
Raden Roro Sri Pudji Sinarni Dewi
Keyword(s):  
Growth Hormone ◽  
Real Time ◽  
Quantitative Pcr ◽  
African Catfish ◽  
Growth Hormone Gene ◽  
Real Time Quantitative Pcr ◽  
Qpcr Method ◽  
Gh Gene ◽  
Pcr Method ◽  
Transgenic Male

In previous study, the fast growth transgenic founder of African catfish was produced harboring a growth hormone (GH) gene construct containing a stripped catfish growth hormone (PhGH) cDNA. This study was conducted to investigate transgene (PhGH) transmission and expression in F-1 transgenic African catfish. The transgenic founders (female) were crossed with non-transgenic (male) to produce heterozygous F-1 progeny. PhGH gene was detected in the embryo, larvae, and seed of the transgenic F-1 using PCR method. Expression levels of transgene in embryo and larvae were analyzed using real-time quantitative PCR (qPCR) method. The transgene was detected in embryo, larvae and seed of F-1 transgenic African catfish. Founder could transmit PhGH gene to transgenic F-1 lines in ranged 36% to 48%. Expression level of Phgh gene in embryo was higher than that of the larvae; whereas in the embryo was 1.5 x 105 - 5.2 x 105 copies or 0.49-9.82 fold, while in the larvae was 1.1 x 105 - 2.5 x 105copies or 0.19-5.80 fold. 

scholarly journals Qtl-Genes in Breeds and Hybrids of Ukrainian Sheep

2020 ◽  
Vol 74 (3) ◽  
pp. 215-221
Author(s):  
Vasyl’ Iovenko ◽  
Yurii Vdjvichenko ◽  
Igor Gorbatenko ◽  
Kostantyn Skrepets ◽  
Ilona Hladii ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Genetic Relationships ◽  
Molecular Genetic ◽  
Live Weight ◽  
Gene Pools ◽  
Growth Hormone Gene ◽  
Homozygous Genotype ◽  
Meat Productivity ◽  
High Level ◽  
Genotype A

AbstractFor the first time, the genetic structure of sheep breeds (Ascanian Fine-Fleeced, Ascanian Meat-and-Wool, AND Ascanian Karakul) and the hybrid Ascanian Fine-Fleeced × Texel was studied for structural gene variants: growth hormone, calpastatin, myostatin, and Booroola bone morphogenetic protein. In all studied groups, sheep were characterised by polymorphism of two loci, growth hormone and calpastatin. Two genotypes (A/A, A/B) represent genes of growth hormone structure, and three (M/M, M/N, N/N) represent genes of calpastatin structure. Other genes are in a monomorphic state. Analysis of the genetic relationships between the studied gene pools showed that there was a definite relationship between productivity of sheep herds and their molecular genetic parameters. Thus, the frequencies of individual genotypes and alleles change in the direction from Fine-Fleeced animals to sheep bred for meat productivity. For example, the heterozygous genotype A/B proportion of growth hormone gene increases in this direction from 0% to 38.2%, and allele A from 0.083 to 0.191. The live weight of Fine-Fleeced lambs with geno-type A/B at birth was 4.5 kg, and with homozygous genotype A/A — 4.9 kg (p < 0.001). This difference is genetically related to the meat productivity of sheep. A similar relationship was established for young Ascanian Karakul sheep. According to the distribution of polymorphic loci variants, the gene pools of Ascanian Meat-and-Wool breed and cross-bred animals are most similar closest among themselves, which is explained by the same direction of their productivity. At the same time, all populations are in genetic equilibrium according to Hardy-Weinberg equations, which indicates a high level of their consolidation. Sheep, which have the A/A homozygote of the gene growth hormone, have increased body weight.

Philogenic Relationship of Wild Pigs and Local Pig from North Sulawesi Based on the Growth Hormone Gene (GH Gene)

2019 ◽  
Vol 967 ◽  
pp. 71-82
Author(s):  
Alexius Mege Revolson ◽  
Yermia Semuel Mokosuli ◽  
Jacqueline Jochebed Rayer Debby ◽  
Ellen Hetie Adil ◽  
Christny Rompas ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Gene ◽  
Wild Pigs ◽  
North Sulawesi ◽  
Extraction And Purification ◽  
Gh Gene ◽  
Pcr Method ◽  
Local Pigs ◽  
Key Words Growth Hormone ◽  
Relationship Of

Growth hormone regulates reproduction and growth in mammals. A study was conducted to obtain the characteristics of the GH gene, in local pigs in North Sulawesi. Pig samples were obtained from traditional farmers, from four districts in North Sulawesi. DNA extraction and purification, using pig pituitary tissue. Amplification of GH gene, performed by PCR method. Visualization of CO1 gene amplikon, performed by electrophoresis technique. Sequencing, conducted through the First BASE Singapore sequencing service. The results show that there is a variation of local pigs CO1 gene in North Sulawesi. Variations are also found in the amino acid sequence encoded by the GH gene. Knowledge of the characteristics of local pig gh gene, the basics of selection of local pigs superior to North Sulawesi. Key words : growth hormone gen, local pigs, Sulawesi Utara Abstrak Gen growth hormone meregulasi reproduksi dan pertumbuhan pada mamalia. Telah dilaksanakan penelitian yang bertujuan untuk mendapatkan karakteristik gen GH pada babi lokal di Sulawesi Utara. Sampel babi diperoleh dari peternak tradisonal dari empat kabupaten di Sulawesi Utara. Ekstraksi dan purifikasi DNA menggunakan jaringan hipofisis babi. Amplifikasi gen GH menggunakan metode PCR. Visualisasi amplikon gen CO1 dilakukan dengan teknik elektroforesis. Sekuensing dilakukan melalui jasa layanan sekuensing First BASE Singapura. Hasil penelitian menunjukkan bahwa terdapat variasi gen CO1 babi lokal di Sulawesi Utara. Variasi juga ditemukan pada urutan asam amino yang dikode oleh gen GH. Diketahuinya karakteristik gen GH babi lokal, menjadi dasar seleksi babi lokal unggul Sulawesi Utara.

scholarly journals IDENTIFIKASI ZIGOSITAS IKAN LELE (Clarias gariepinus) TRANSGENIK F-2 YANG MEMBAWA GEN HORMON (PhGH) DENGAN MENGGUNAKAN METODE REALTIME-qPCR

2016 ◽  
Vol 11 (1) ◽  
pp. 39
Author(s):  
Huria Marnis ◽  
Bambang Iswanto ◽  
Romy Suprapto ◽  
Imron Imron ◽  
Raden Roro Sri Pudji Sinarni Dewi
Keyword(s):  
Real Time ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
Progeny Test ◽  
African Catfish ◽  
Mendelian Segregation ◽  
Qpcr Method ◽  
Pcr Method ◽  
Real Time Qpcr ◽  
Transgene Transmission

Produktivitas ikan budidaya dapat ditingkatkan melalui teknologi transgenesis. Populasi ikan lele transgenik cepat tumbuh telah dihasilkan dan karakter biologisnya telah diketahui. Namun informasi zigositas ikan lele transgenik perlu ditelaah lebih lanjut. Penelitian ini bertujuan untuk mengidentifikasi zigositas ikan lele transgenik F-2. Zigositas ikan lele transgenik diidentifikasi dengan menggunakan metode real-time qPCR (RT-qPCR) dan uji progeni. Identifikasi zigositas melalui uji progeni, dilakukan dengan mendeteksi transgen (PhGH) pada individu-individu F-3 hasil persilangan transgenik F-2 dengan non-transgenik. Hasil penelitian menunjukkan bahwa zigositas pada ikan lele transgenik F-2 dapat diidentifikasi dengan menggunakan metode RT-qPCR. Semua ikan transgenik F-2 adalah heterozigot, dengan nilai 2-Ct yang hampir sama tiap individu F-2, yaitu berkisar 0,80-0,99. Identifikasi zigositas dengan metode RT-qPCR menunjukkan hasil yang sama dengan uji progeni, semua transgenik F-2 tidak menghasilkan 100% anakan F-3 positif transgen. Pada uji progeni, transmisi transgen pada penelitian ini tidak mengikuti hukum segregasi Mendel, dengan kisaran sebesar 5%-40%.Fish farming productivity can be increased by transgenesis technology. On the previous study, transgenic African catfish population fast growing has been produced and its biological characters has been known. However information of transgenic zygosity of catfish should be examined. The aim of this study was to identify the zygosity of F-2 transgenic African catfish. The zygosity of F-2 transgenic was identified by real time-qPCR (RT-qPCR) method and progeny test. Further, identification of zygosity F-2 transgenic African catfish was confirmed by progeny test, while F-2 transgenic African catfish was mated with non-transgenic. Identification of zygosity F-2 transgenic was conducted by detection PhGH gene (transgene) in F-3 transgenic African catfish population. Transgene transmission was evaluated by PCR method. The result showed that the zygosity F-2 transgenic African catfish could be identified by RT-qPCR method. All F-2 transgenic African catfish were heterozygous, where as the 2-Ct value was almost same for all individual, which ranges from 0.80 to 0.99. The result of zygosity identification using RT-qPCR method was as same as that of progeny test. In the progeny test, transgene transmission in this study was non-Mendelian segregation, with ranges of 5%-40%.

scholarly journals Antibacterial Activity of Isolates from Digestive Tract of Catfish (Clarias gariepinus) As Probiotic Candidates At PT. Biotech Cipta Kreasi

2021 ◽  
Vol 3 (1) ◽  
pp. 138-149
Author(s):  
Toto Apriliyanto ◽  
Nosa Septiana Anindita ◽  
Wisnu Adhi Susila
Keyword(s):  
Digestive Tract ◽  
Aeromonas Hydrophila ◽  
Low Cost ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
African Catfish ◽  
Bacillus Sp ◽  
High Level ◽  
Control Management ◽  
Probiotic Activity

African catfish (Clarias gariepinus) is a popular fish in the community because it provides a high level of protein at a low cost. The motile aeromonas septicemia (MAS) disease produced by Aeromonas hydrophila (A. hydrophila) infection is one of the things that create difficulties in the cultivation of this fish. Synthetic antibiotics are commonly used in MAS disease control management efforts in fish farming, which have have an influence not only on fish but also on consumers. The application of probiotic Bacillus sp isolated from the digestive tract of catfish as a means of preventing the sickness is one option. Enzymatic tests (lipolytic, amylolytic, cellulolytic, proteolytic activity) and antagonist testing were used to assess the probiotic activity of the Bacillus sp isolates.

scholarly journals Anomalies in Bighead Carp Aristichthys nobilis and African Catfish Clarias gariepinus in Biratnagar, Nepal

Our Nature ◽  
1970 ◽  
Vol 2 (1) ◽  
pp. 41-44
Author(s):  
B.R. Subba
Keyword(s):  
Key Words ◽  
Vertebral Column ◽  
Clarias Gariepinus ◽  
Bighead Carp ◽  
African Catfish ◽  
Caudal Fin ◽  
Aristichthys Nobilis ◽  
Upper Jaw

Truncation and deformation on the upper jaw in juvenile and adult respectively in African catfish Clarias gariepinus; and vertebral abnormalities in Bighead carp Aristichthys nobilis are described. Key words: Anomalies, Upper jaw, Caudal fin, Vertebral column, Fishesdoi:10.3126/on.v2i1.324Our Nature (2004) 2: 41-44

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