scholarly journals aplikasi protein sel tunggal dan “spent grains” dalam FORMULASI PAKAN untuk pemeliharaan kerapu hibrid (Epinephelus fuscoguttatus x Epinephelus polypekadion)

2018 ◽  
Vol 13 (1) ◽  
pp. 41
Author(s):  
Wawan Andriyanto ◽  
Nyoman Adiasmara Giri ◽  
Muhammad Marzuqi

Sumber protein alternatif berupa protein sel tunggal (PST) dari sisa produksi bumbu penyedap dan “spent grains” dari sisa produksi minuman bir memiliki kandungan protein yang tinggi. Penelitian ini bertujuan untuk mendapatkan dosis terbaik dari penggunaan dua sumber bahan tersebut terhadap pertumbuhan dan sintasan ikan kerapu hibrid (kerapu cantik). Empat pakan uji diformulasikan dengan menambahkan kombinasi tepung spent grains dan PST untuk menggantikan protein dari tepung ikan sebesar (A) 0%, (B) 30%, (C) 40%, dan (D) 50%. Uji pakan dilakukan di keramba jaring apung menggunakan 12 jaring berukuran 2 m x 2 m x 2 m. Bobot awal ikan kerapu cantik yang digunakan adalah 63 ± 0,6 g dan ditebar dengan kepadatan 253 ekor/jaring. Pakan uji diberikan dua kali sehari secara satiasi. Data yang diamati adalah pertumbuhan (bobot dan panjang), sintasan, dan konversi pakan. Hasil penelitian menunjukkan bahwa perlakuan pakan A (0%) menghasilkan berat akhir sebesar 299,6 ± 27,0 g lebih baik dan berbeda nyata (P<0,05) dari perlakuan C (40%) dan D (50%), namun tidak berbeda nyata (P>0,05) dengan perlakuan pakan B (30%), yaitu 287,4 ± 5,3 g. Hasil ini menunjukkan bahwa substitusi protein tepung ikan dengan campuran protein dari PST dan spent grains pada pakan pembesaran kerapu hibrid dapat dilakukan sampai level 30%.Single cell protein left-over from seasoning factory and spent grains from brewery waste are potential as high protein sources for animal feed. This study was aimed to obtain an optimal supplementation dose of the two ingredients in relation to the growth and survival rate of hybrid grouper (Cantik grouper). Four test feeds were formulated by mixing different combinations of single cell protein and spent grains to replace fishmeal protein content in the feed at 0%, 30%, 40%, and 50%. The feeding trials were conducted at a floating net cage consisted of 12 netpens sized 2 m x 2 m x 2 m. The initial mean weight of hybrid grouper was 63 ± 0.6 g. The fish were stocked at a density of 253 fish/netpen. Feed treatments were given at satiation two times a day. Parameters of growth (final body weight and length), survival rate, and feed conversion were observed. The results showed that fish in treatment A (0%) gained the final body weight of 299.6 ± 27.01 g which was significantly different (P<0.05) with that of treatment C and D, but not significantly different with fish in treatment B (30%), with the mean weight gain of 287.4 ± 5,3 g. Fish in treatment B (30%) showed better growth response and survival rate than that of treatment C (40%) and D (50%). The result also indicated that fish fed with treatment B feed (30%) had not different a mean final body weight with the control feed. This study suggested that a combination of single cell protein and spent grains to substitute fish meal in grow-out feed for hybrid grouper could be applied up to 30%.

2012 ◽  
Vol 12 (1) ◽  
pp. 7-15
Author(s):  
Samadi Samadi ◽  
Mira Delima ◽  
Zuraida Hanum ◽  
Muhammad Akmal

The effect of level substitution of single cell protein (CJ Prosin) by commercial feed on broiler performanceABSTRACT. A research to find out the effect of the substitution level of single cell protein in commercial feed on Broiler’s performance was conducted from June to December 2011. The purpose of this experiment is to find out alternative protein sources for animal feedstuff that can be used as protein substitution in animal feed on body weight, feed consumption and feed conversion efficiency. The experiment is considered as preliminary experiment to observe the response of single cell protein on broiler performance. This research was carried out at experimental farm, Animal Husbandry Department, Syiah Kuala University. Totally, 250 DOC of Cobb breed with the average body weight 36,78±2,32 gr. used in this experiment. Completely Randomized Design was applied in this experiment with 5 treatments; P0 (commercial feed), P1(commercial feed substituted with 6% SCP), P2 (commercial feed substituted with 12% SCP) dan P3 (commercial feed substituted with 18%CSP). Each treatment consist of 5 replications with total of 20 treatment’s units. Feed was formulated with isoprotein and isoenergy based on NRC requirement ( 23% protein ; 3200 kcal ME/kg at the age of 1-2 week and 20 % protein ; 3200 kcal ME/kg at the age of 4-7 week). Data was analyzed by using SPSS program SPSS (Window 2008). Differences between treatment were stated by Duncan’s multiple range test. The results of the experiment showed that commercial feed substituted with the level of SCP was not significantly difference (P0.05) on body weight gain, feed consumption, and feed conversion ratio. On the other hand, administration of SCP as substituted feed commercial on the level of 12 % and 16 % influenced significantly (P0.05) on body weight gain and feed conversion ratio, but not on feed consumption at the level of 12% SCP. As a conclusion, the use of SCP as animal feed sources in commercial feed only could be tolerated at the level of 6%.


2016 ◽  
Vol 14 (3) ◽  
pp. 581-588
Author(s):  
Nguyễn Thị Hiếu Thu ◽  
Đinh Thuý Hằng

Single cell protein (SCP) can be produced from biomass of different types of microorganisms that have high protein content such as yeast, filamentous fungi, algae and bacteria. In comparison to animal and plant protein sources, this kind of protein has several advantages, namely high protein and nutrient contents, being produced in fermenters with the use of variety of organic wastes, independence in agriculture land or climate conditions. Methane oxidizing bacteria (MOB) are considered as good candidates for SCP production and have been intensively studied recently. In the present study, a MOB strain BG3 was isolated from wastewater of an anaerobic digester via enrichment and isolation procedures using methane as the only carbon and energy sources. Strain BG3 comprised of oval-shaped cells of 0,8-1´16 -1,8 μm in size, almost nonmotile. Based on comparative analyses of the 16S rDNA partial sequences, strain BG3 was identified as a member of the Methylomonas genus, the most closely related species was Methylomonas koyamae (97% homology). This was also confirmed by analyses of sequence of the pmoA gene, encoding for a-subunit in the methane-monooxygenase in the strain.  Besides methane, strain BG3 also utilized methanol for the growth. It has been shown that methane-fed culture of strain BG3 could produce 68.69 g crude protein per 100 g CDW and the according methane to biomass conversion efficiency was 2,8 m3 methane×kg-1 dry biomassas. Owing the capability of utilization of methane, the second important greenhouse gas, for the production of protein source for animal feed, strain BG3 would have a great application potential in Vietnam. Strain BG3 was designated as Methylomonas sp. BG3 and its 16S rDNA and pmoA gene sequences were deposited at the GenBank with accession numbers of KJ081955 and KJ081956, respectively.


Author(s):  
I. Y. Hamdan ◽  
H. N. Asthana ◽  
N. Al-Awadi ◽  
A. S. ElNawawy ◽  
I. Banat ◽  
...  

Single cell proteins are rich essentialin nutritive aminoacids,the building blocks of protein are highly essential for the maintenance of the living system. SCP is used as animal feed and dietary rich food for humans. Many raw materials are used for the production of SCP. This work was carried out to extract a single cell protein from yeast using Vignaunguriculataand Cicerarietinums substrate. The maximum yield of crude protein was observed in 15days of fermentation


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