Expression of <i>Oryza sativa</i> galactinol synthase gene  in maize (Zea may L.)

Galactinol synthase (GolS) is a key biological catalyst for the synthesis of the raffinose oligosaccharides (RFOs) which play important roles in abiotic stress adaptation of plants, especially drought tolerance. GolS gene has been isolated on a variety of plants in order to create material resources for generating transgenic plants resistant to adverse environmental factors. In our previous research, we have isolated a GolS gene from drought stress cDNA library of Oryza sativa L. Moctuyen (named OsGolS). In this study, the expression vector pCAM-Rd/OsGolS carrying the isolated OsGolS gene under the control of stress-inducible Rd29A promoter was constructed and introduced into Agrobacterium tumefaciens LBA4404, which was used for maize transformation. PCR and Real-time PCR assay indicated that transgene was integrated in the genome of the regenerated Zea mays plants. Reverse transcription-PCR showed that the OsGolS was transcribed into mRNA in Zea mays and was highly expressed. These results provide a basis for the study of the function of OsGolS in drought responses and for the development of drought stress tolerant crops.

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Assessment of rice (Oryza sativa L.) genotypes for drought stress tolerance using morpho-physiological indices as a screening technique

Pakistan Journal of Botany ◽

10.30848/pjb2021-1(33) ◽

2020 ◽

Vol 53 (1) ◽

Author(s):

Asma Asma ◽

Iqbal Hussain ◽

Muhammad Yasin Ashraf ◽

Muhammad Arslan Ashraf ◽

Rizwan Rasheed ◽

...

Keyword(s):

Oryza Sativa ◽

Drought Stress ◽

Stress Tolerance ◽

Oryza Sativa L ◽

Screening Technique ◽

Drought Stress Tolerance ◽

Physiological Indices

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Retraction: “Screening of candidate genes and fine mapping of drought tolerance quantitative trait loci on chromosome 4 in rice (Oryza sativa L.) under drought stress” by Nie, Y. Y., L. Zhang, Y. H. Wu, H. J. Liu, W. W. Mao, J. Du, et al.

Ecology and Evolution ◽

10.1002/ece3.1855 ◽

2015 ◽

Vol 6 (1) ◽

pp. 375-375

Keyword(s):

Oryza Sativa ◽

Drought Stress ◽

Quantitative Trait Loci ◽

Drought Tolerance ◽

Candidate Genes ◽

Fine Mapping ◽

Quantitative Trait ◽

Oryza Sativa L ◽

Chromosome 4 ◽

Trait Loci

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Water uptake dynamics under progressive drought stress in diverse accessions of the OryzaSNP panel of rice (Oryza sativa)

Functional Plant Biology ◽

10.1071/fp12015 ◽

2012 ◽

Vol 39 (5) ◽

pp. 402 ◽

Cited By ~ 28

Author(s):

Veeresh R. P. Gowda ◽

Amelia Henry ◽

Vincent Vadez ◽

H. E. Shashidhar ◽

Rachid Serraj

Keyword(s):

Oryza Sativa ◽

Drought Stress ◽

Root Growth ◽

Water Uptake ◽

Root Length Density ◽

Oryza Sativa L ◽

Root Water Uptake ◽

Snp Markers ◽

Drought Response ◽

Genotypic Differences

In addition to characterising root architecture, evaluating root water uptake ability is important for understanding drought response. A series of three lysimeter studies were conducted using the OryzaSNP panel, which consists of 20 diverse rice (Oryza sativa L.) genotypes. Large genotypic differences in drought response were observed in this genotype panel in terms of plant growth and water uptake. Total water uptake and daily water uptake rates in the drought-stress treatment were correlated with root length density, especially at depths below 30 cm. Patterns of water uptake among genotypes remained consistent throughout the stress treatments: genotypes that initially extracted more water were the same genotypes that extracted more water at the end of the study. These results suggest that response to drought by deep root growth, rather than a conservative soil water pattern, seems to be important for lowland rice. Genotypes in the O. sativa type aus group showed some of the greatest water uptake and root growth values. Since the OryzaSNP panel has been genotyped in detail with SNP markers, we expect that these results will be useful for understanding the genetics of rice root growth and function for water uptake in response to drought.

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