Assessment of bacterial cellulose membrane produced by Acetobacter xylinum used as scaffold for mouse fibroblast culture

In recent years, bacterial cellulose material has been considered as a potential biotechnological product for biomedical applications. Previous studies described some special properties of bacterial cellulose, such as water holding capacity, high polymerization, high crystallization, high purity, and strength. In this study, bacterial cellulose membrane produced by Acetobacter xylinum was examined for its possibility to use as a scaffold for cell to grow. Firstly, mechanical properties of bacterial cellulose membrane including strength, stress at break, strain at break, and modulus were analyzed. Secondly, cellulose fiber structure was observed with scanning electron microscope. Lastly, biocompatibility of bacterial cellulose membrane was investigated for application as scaffold for cell culture. The results showed that bacterial cellulose membrane had fine fibres arranged to form 3-D porous structured hydrogel. Also, the mechanical qualities of material were suitable for using as a biomaterial. Fibroblast cells isolated from mouse’s skin and tail bone were cultured in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum and 1% antibiotics. Cells then collected and sew into bacterial cellulose membrane placed in cell culture disk. At different time points at 1 day, 4 days, and 7 days after sowing the cells, it is clearly seen that cells can adhere, grow and expand on the surface of cellulose membrance placed on cell culture disk, as comparible as cells cultured in disk without cellulose membrance. In conclusion, bacterial cellulose membrane is a suitable material for cell culture as a scaffold. The results observed from this study might be suggestions for next investigations on using bacterial cellulose membrane as scaffold for tissue engineering.

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X-ray Diffraction Diagram of the Bacterial Cellulose Membrane Produced by Acetobacter xylinum in the Medium with Lignosulfonate.

Sen i Gakkaishi ◽

10.2115/fiber.52.169 ◽

1996 ◽

Vol 52 (4) ◽

pp. 169-174 ◽

Cited By ~ 5

Author(s):

Siripong Premjet ◽

Yoshito Ohtani ◽

Kazuhiko Sameshima

Keyword(s):

Bacterial Cellulose ◽

Acetobacter Xylinum ◽

Cellulose Membrane ◽

X Ray Diffraction ◽

X Ray ◽

Bacterial Cellulose Membrane ◽

Diffraction Diagram

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Biosynthesis of Cellulose by Acetobacter Xylinum. III. X-Ray Studies of Preferential Orientation of the Crystallites in a Bacterial Cellulose Membrane

Polymer Journal ◽

10.1295/polymj.7.157 ◽

1975 ◽

Vol 7 (2) ◽

pp. 157-164 ◽

Cited By ~ 25

Author(s):

Mitsuo Takai ◽

Yasushi Tsuta ◽

Jisuke Hayashi ◽

Sadayoshi Watanabe

Keyword(s):

Bacterial Cellulose ◽

Acetobacter Xylinum ◽

Preferential Orientation ◽

Cellulose Membrane ◽

X Ray ◽

Bacterial Cellulose Membrane

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Fabrication of bacterial cellulose membrane-based alkaline-exchange membrane for application in electrochemical reduction of CO2

Separation and Purification Technology ◽

10.1016/j.seppur.2021.118910 ◽

2021 ◽

pp. 118910

Author(s):

Qianqian Zou ◽

Xiaojing Guo ◽

Lu Gao ◽

Feng Hong ◽

Jinli Qiao

Keyword(s):

Bacterial Cellulose ◽

Electrochemical Reduction ◽

Cellulose Membrane ◽

Bacterial Cellulose Membrane ◽

Reduction Of Co2 ◽

Exchange Membrane

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Bacterial Cellulose Membrane Containing Epilobium angustifolium L. Extract as a Promising Material for the Topical Delivery of Antioxidants to the Skin

International Journal of Molecular Sciences ◽

10.3390/ijms22126269 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6269

Author(s):

Anna Nowak ◽

Paula Ossowicz-Rupniewska ◽

Rafał Rakoczy ◽

Maciej Konopacki ◽

Magdalena Perużyńska ◽

...

Keyword(s):

Bacterial Cellulose ◽

Topical Application ◽

Phenolic Acid ◽

Antioxidant Properties ◽

Ethanolic Extract ◽

Cellulose Membrane ◽

Hydroxybenzoic Acid ◽

Epilobium Angustifolium ◽

Bacterial Cellulose Membrane

Bacterial cellulose membranes (BCs) are becoming useful as a drug delivery system to the skin. However, there are very few reports on their application of plant substances to the skin. Komagataeibacter xylinus was used for the production of bacterial cellulose (BC). The BC containing 5% and 10% ethanolic extract of Epilobium angustifolium (FEE) (BC-5%FEE and BC-10%FEE, respectively) were prepared. Their mechanical, structural, and antioxidant properties, as well as phenolic acid content, were evaluated. The bioavailability of BC-FESs using mouse L929 fibroblasts as model cells was tested. Moreover, in vitro penetration through the pigskin of the selected phenolic acids contained in FEE and their accumulation in the skin after topical application of BC-FEEs was examined. The BC-FEEs were characterized by antioxidant activity. The BC-5% FEE showed relatively low toxicity to healthy mouse fibroblasts. Gallic acid (GA), chlorogenic acid (ChA), 3,4-dihydroxybenzoic acid (3,4-DHB), 4-hydroxybenzoic acid (4-HB), 3-hydroxybenzoic acid (3-HB), and caffeic acid (CA) found in FEE were also identified in the membranes. After topical application of the membranes to the pigskin penetration of some phenolic acid and other antioxidants through the skin as well as their accumulation in the skin was observed. The bacterial cellulose membrane loaded by plant extract may be an interesting solution for topical antioxidant delivery to the skin.

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Bacterial Cellulose as a Substrate for Microbial Cell Culture

Applied and Environmental Microbiology ◽

10.1128/aem.03452-13 ◽

2014 ◽

Vol 80 (6) ◽

pp. 1926-1932 ◽

Cited By ~ 17

Author(s):

Na Yin ◽

Thiago M. A. Santos ◽

George K. Auer ◽

John A. Crooks ◽

Piercen M. Oliver ◽

...

Keyword(s):

Cell Culture ◽

Cell Growth ◽

Physicochemical Properties ◽

Stationary Phase ◽

Bacterial Cellulose ◽

Growth Rates ◽

Bacterial Cell ◽

Acetobacter Xylinum ◽

Primary Factor ◽

Nutrient Diffusion

ABSTRACTBacterial cellulose (BC) has a range of structural and physicochemical properties that make it a particularly useful material for the culture of bacteria. We studied the growth of 14 genera of bacteria on BC substrates produced byAcetobacter xylinumand compared the results to growth on the commercially available biopolymers agar, gellan, and xanthan. We demonstrate that BC produces rates of bacterial cell growth that typically exceed those on the commercial biopolymers and yields cultures with higher titers of cells at stationary phase. The morphology of the cells did not change during growth on BC. The rates of nutrient diffusion in BC being higher than those in other biopolymers is likely a primary factor that leads to higher growth rates. Collectively, our results suggest that the use of BC may open new avenues in microbiology by facilitating bacterial cell culture and isolation.

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