scholarly journals SEPARATION AND HPLC QUANTITATIVE ANALYSIS OF MURRAYAFOLINE A FROM THE ROOTS OF GLYCOSMIS STENOCARPA

2018 ◽  
Vol 54 (2C) ◽  
pp. 530
Author(s):  
Tran Quoc Toan
Keyword(s):  
Quantitative Analysis ◽  
Column Chromatography ◽  
Steam Distillation ◽  
Hplc Method ◽  
Distillation Method ◽  
Separation And Purification ◽  
Plant Materials

In this study, we investigated two methods for the separation and purification of alkaloidMurrayafoline A from the roots of the Vietnamese plant Glycosmis stenocarpa and developed anHPLC method for the determination of its contents in plant materials and the methanol extract.The yield of Murrayafoline A using steam distillation method was 1.5 times higher than theseparation method using column chromatography. By HPLC method, the content ofMurrayafoline A was determined to be 0.38 % (w/w) in the roots and 16.69 % (w/w) in themethanol extract of G. stenocarpa.

High-Speed Liquid Chromatographic Determination of o-Phenylphenol Residues in Citrus Products

1976 ◽  
Vol 59 (1) ◽  
pp. 162-164
Author(s):  
Samuel K Reeder
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Speed ◽  
Steam Distillation ◽  
Chromatographic Determination ◽  
Analysis Time ◽  
Hexane Extraction ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method is presented for the quantitative analysis of o-phenylphenol residues in citrus oils, encapsulated flavors, and dried meal. The method utilizes high-speed liquid chromatography for the determination after specific sample preparations for each material. These preparations include hexane extraction of acidified basic extracts or steam distillation and extraction. The limit of the analysis is <1 ppm with an analysis time of <45 min.

scholarly journals Quantitative Determination of Pregnanes from Aerial Parts of Caralluma Species Using HPLC-UV and Identification by LC-ESI-TOF

2011 ◽  
Vol 94 (5) ◽  
pp. 1383-1390
Author(s):  
Bharathi Avula ◽  
Yatin J Shukla ◽  
Yan-Hong Wang ◽  
Ikhlas A Khan
Keyword(s):  
Quantitative Determination ◽  
Dietary Supplements ◽  
Hplc Method ◽  
Photodiode Array Detection ◽  
Plant Materials ◽  
Aerial Parts ◽  
Photodiode Array ◽  
Array Detection ◽  
Na Ions

Abstract An HPLC method was developed for the quantitative determination of five pregnane derivatives from aerial parts of Caralluma species and dietary supplements. The method was validated for linearity, repeatability, LOD, and LOQ. The LOD and LOQ of five pregnane compounds were found to be in the range of 1–5 and 3–15 μg/mL, respectively, by HPLC using photodiode array detection. This method was applied to the identification of three plant materials of Caralluma species (C. fimbriata, C. umbellate, and C. attentuata) and seven dietary supplements claiming to contain C. fimbriata. An LC/MS coupled with electrospray ionization interface method was used for the identification of compounds and involved the use of [M+Na]+ ions in the positive ion mode with extracted ion chromatogram.

scholarly journals Simultaneous Determination of Four Major Constituents of Semen Vaccariae Using HPLC-DAD

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Haijiang Zhang ◽  
Wei Yao ◽  
Yunyun Chen ◽  
Peipei He ◽  
Yao Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Hplc Method ◽  
Frying Temperature ◽  
Simultaneous Quantification ◽  
Frying Process

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.

Determination of Formaldehyde in Water-Borne Coatings by HPLC

2017 ◽  
Vol 726 ◽  
pp. 355-359 ◽  
Author(s):  
Ming Yu Wang ◽  
Zhong Bao Guo ◽  
Hong Yan Guan ◽  
Man Li
Keyword(s):  
Extraction Method ◽  
High Performance ◽  
Steam Distillation ◽  
High Performance Liquid Chromatographic ◽  
Distillation Method ◽  
Efficient Extraction ◽  
Liquid Chromatographic ◽  
Good Linear Correlation ◽  
Water Borne

A method for determination of formaldehyde in water-borne coatings by high-performance liquid chromatographic was proposed. Ultrasonic water extraction method was used instead of the traditional steam distillation method can be more rapid and efficient extraction of target, filtered and derivatived with DNPH. The extract was separated by C18 column with an elution composed of acetonitrile and water, and detected at 352nm. The detection limit was 0.1mg/L. The method showed good linear correlation, R2 was 0.9997 at the range of 0.1mg/L ~ 20 mg/L. Recovery was 95% ～105%. This method is simple, effective and sensitive.

scholarly journals Comparative Study on Quantification of Total Catechins Using UV-Vis Spectrophotometric Method and High Performance Liquid Chromatography Techniques

2021 ◽  
Vol 37 (1) ◽  
pp. 136-142
Author(s):  
Sitharanjan Kalidass ◽  
Karuppannau Daiyarvijaya ◽  
Rajagopal Raj Kumar
Keyword(s):  
Quantitative Analysis ◽  
Spectrophotometric Method ◽  
Mobile Phase ◽  
High Performance ◽  
Cost Effective ◽  
Hplc Method ◽  
Good Resolution ◽  
Huge Number ◽  
Automated Hplc

Bioavailability of catechinsin wider range of plants was established earlier and it’s utility as medicine against cardiovascular disease, cancer, etc. were also demonstrated. Recent techniques in relation to quantitative analysis of total catechins seem to be laborious and time consuming process to handle huge number of samples. Established spectrophotometry and HPLC methods developed earlier for quantitative determination of total catechins in tea extracts were compared in the present study.UV-Vis spectrophotometric method was adopted to monitor the absorbance at 500 nm of the reaction mixture (catechins and vanillin-H2SO4reagents). Hewlett Packard automated HPLC was used and equipped with Phenomenex Luna 5  phenyl-hexyl column fitted with a Phenomenex guard column. Binary elution was carried out using Mobile phase A (acetic acid and acetonitrile) and Mobile phase B (acetonitrile). Method adopted showed a good resolution of catechin fractions and was found to be accurate for the quantification of total catechins (sum of individual catechins). Results of the both the methods are comparable and variation amongst the two methods ranged between -3.59 and 2.79% among the clones and varied with seasons.As expected UPASI released tea clones exhibited variations in their bioavailability. Lean season edge over the cropping period sampling in terms of total catechins. Results obtained from both the methods are comparable. Two methods can be used for the routine quantitative analysis of total catechins; however, spectrophotometric method found to be simple, rapid and cost effective than that of HPLC method unless individual catechins composition is warranted.

Analysis of Sulfites in Shrimp Using Rapid Distillation Followed by Redox Titration1

1988 ◽  
Vol 51 (2) ◽  
pp. 137-138 ◽  
Author(s):  
MARIAN V. SIMPSON ◽  
W. STEVEN OTWELL ◽  
MAURICE R. MARSHALL ◽  
JOHN A. CORNELL
Keyword(s):  
Statistical Analysis ◽  
Steam Distillation ◽  
Accurate Determination ◽  
T Test ◽  
Distillation Method ◽  
Student’S T ◽  
Student’S T Test

The use of rapid steam distillation followed by redox iodine titration provides a rapid and accurate determination of total sufite residual in shrimp. Values obtained for sulfite-treated shrimp using the rapid distillation method gave comparable results to those of the officially recognized Monier-Williams method. Values for the rapid distillation method ranged from 6 to 212 ppm while those of the Monier-Williams procedure ranged from 6 to 241 ppm for untreated and treated shrimps, respectively. Statistical analysis using two-sample Student's t-test indicated that there were no significant differences (p>0.05) for residual levels below 100 ppm but the values obtained by the rapid distillation method and the Monier-Williams procedure were significantly different (p<0.05) at concentrations near and above 100 ppm.

scholarly journals Quantitative Analysis of Eugenol in Clove Extract by a Validated HPLC Method

2010 ◽  
Vol 93 (6) ◽  
pp. 1806-1810 ◽  
Author(s):  
So-Mi Yun ◽  
Myoung-Heon Lee ◽  
Kwang-Jick Lee ◽  
Hyun-Ok Ku ◽  
Seong-Wan Son ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Correlation Coefficients ◽  
Hplc Method ◽  
Marker Compound ◽  
Safety Assurance ◽  
Chemical Evaluation ◽  
Array Detection ◽  
Digestive Disorders ◽  
Development And Validation

Abstract Clove (Eugenia caryophyllata) is a well-known medicinal plant used for diarrhea, digestive disorders, or in antiseptics in Korea. Eugenol is the main active ingredient of clove and has been chosen as a marker compound for the chemical evaluation or QC of clove. This paper reports the development and validation of an HPLC-diode array detection (DAD) method for the determination of eugenol in clove. HPLC separation was accomplished on an XTerra RP18 column (250 4.6 mm id, 5m) with an isocratic mobile phase of 60 methanol and DAD at 280 nm. Calibration graphs were linear with very good correlation coefficients (r2 > 0.9999) from 12.5 to 1000 ng/mL. The LOD was 0.81 and the LOQ was 2.47 ng/mL. The method showed good intraday precision (RSD 0.080.27) and interday precision (RSD 0.321.19). The method was applied to the analysis of eugenol from clove cultivated in various countries (Indonesia, Singapore, and China). Quantitative analysis of the 15 clove samples showed that the content of eugenol varied significantly, ranging from 163 to 1049 ppb. The method of determination of eugenol by HPLC is accurate to evaluate the quality and safety assurance of clove, based on the results of this study.

scholarly journals Some Fundamental Experiments on the Differential Determination of Several Kinds of Preservatives in Foods by Steam Distillation Method

1966 ◽  
Vol 7 (2) ◽  
pp. 134-139
Author(s):  
Saburo KANNO ◽  
Shogo OCHIAI ◽  
Tadayoshi NAKAOKA ◽  
Tetsuo INOUE ◽  
Taro KAWAMURA ◽  
...  
Keyword(s):  
Steam Distillation ◽  
Distillation Method

scholarly journals Quantitative Determination of Arbutin and Hydroquinone in Different Plant Materials by HPLC

2012 ◽  
Vol 40 (2) ◽  
pp. 109 ◽  
Author(s):  
Izabela RYCHLINSKA ◽  
Slawomira NOWAK
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Hplc Method ◽  
Fast Method ◽  
Optimum Conditions ◽  
Plant Materials ◽  
Ledum Palustre ◽  
Relative Standard ◽  
First Time

A simple, fast method of high-performance liquid chromatography for the determination and quantification of arbutin and hydroquinone in many different raw materials was developed and validated. The optimum conditions for the separation and detection of these two constituents were achieved on a LiChro-CARD 125-4 Superspher®100 RP-18 column with the water-methanol (gradient elution) mobile phase and recorded at 289 nm. The purities of peaks were verified by PDA analysis of impurities. The results of validation have shown that the HPLC method is stable and accurate for the simultaneous determination of arbutin and hydroquinone in extracts from various plants. The developed method gave a good sensitivity (LOD 1µg/ml for arbutin and 0.49 µg/ml for hydroquinone) with linearity R2 >0.9993 (for both). The relative standard deviation of the method was less than 2.53% for intra-day assays and 3.23% for inter-day assay, the accuracy of the recovery test ranged from 98.96% to 106.4%. This method was used in comparative qualitative analysis of arbutin and hydroquinone in 16 different raw materials from families Lamiaceae, Ericacaeae, Saxifragaceae, Rosaceae. The content of arbutin in B. ciliata, B. cordifolia and Ledum palustre was examined for the first time.

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