scholarly journals The small bowel microbiome changes significantly with age and aspects of the ageing process

2022 ◽  
Vol 9 (1) ◽  
pp. 21-23
Author(s):  
Gabriela Leite ◽  
Mark Pimentel ◽  
Gillian M. Barlow ◽  
Ruchi Mathur
Keyword(s):  
Small Intestine ◽  
Small Bowel ◽  
Intestinal Microbiome ◽  
Ageing Process ◽  
Age Related ◽  
Human Ageing ◽  
Stool Samples ◽  
Older Subjects ◽  
Small Intestinal ◽  
Host Metabolism

Gut microbiome changes have been associated with human ageing and implicated in age-related diseases including Alzheimer’s disease and Parkinson’s disease. However, studies to date have used stool samples, which do not represent the entire gut. Although more challenging to access, the small intestine plays critical roles in host metabolism and immune function. In this paper (Leite et al. (2021), Cell Reports, doi: 10.1016/j.celrep.2021.109765), we demonstrate significant differences in the small intestinal microbiome in older subjects, using duodenal aspirates from 251 subjects aged 18-80 years. Differences included significantly decreased microbial diversity in older subjects, driven by increased relative abundance of phylum Proteobacteria, particularly family Enterobacteriaceae and coliform genera Escherichia and Klebsiella. Moreover, while this decreased diversity was associated with the ‘ageing process’ (comprising chronologic age, number of medications, and number of concomitant diseases), changes in certain taxa were found to be associated with number of medications alone (Klebsiella), number of diseases alone (Clostridium, Bilophila), or chronologic age alone (Escherichia, Lactobacillus, Enterococcus). Lastly, many taxa associated with increasing chronologic age were anaerobes. These changes may contribute to changes in human health that occur during the ageing process.

Ontogenetic development of monosaccharide and amino acid transporters in rabbit intestine

1990 ◽  
Vol 259 (4) ◽  
pp. G544-G555 ◽  
Author(s):  
R. K. Buddington ◽  
J. M. Diamond
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Metabolic Rate ◽  
Direct Proportion ◽  
Natural Diet ◽  
Sugar Absorption ◽  
Age Related ◽  
Rabbit Intestine ◽  
Small Intestinal ◽  
Rabbit Milk

We measured brush-border uptakes of seven sugars and amino acids by rabbit intestine as a function of age from the day of birth to adulthood. Gut dimensions, especially those of the colon and cecum, increase more rapidly with body weight than would be true if rabbits maintained identical proportions as they grew. However, nominal small intestinal area increases in approximately direct proportion to the animal's basal metabolic rate. For all solutes except fructose, uptake per milligram of intestinal tissue is maximal at or near birth and declines to a level 2.5-5 times lower in the adult. Because of small intestinal growth, though, the total uptake capacity of the whole length of the small intestine increases in approximately direct proportion to metabolic rate. Fructose uptake per milligram is unique in increasing steeply at the time of weaning, correlated with the post-weaning first appearance of fructose in the natural diet. Age-related changes in uptake ratios among aldohexoses or amino acids suggest developmental sequences of related transporters. Correlated with the very high protein content of rabbit milk, the proline-to-glucose uptake ratio is higher in suckling rabbits than in other sucking mammals. Remarkably, the ratio for adult rabbits is higher than in other monogastric herbivores and is instead similar to values for carnivores. In explanation, although the transport capacity of the small intestine appears to account for proline absorption in rabbits of all ages and for sugar absorption in suckling rabbits, the hindgut may be a major site of carbohydrate digestion in adult rabbits.

scholarly journals Age-related histochemical investigations of small intestinal goblet cells in bronze turkeys (Meleagris gallopavo gallopavo)

2021 ◽  
Vol 24 (2) ◽  
pp. 176-183
Author(s):  
D. Yovchev ◽  
G. Penchev
Keyword(s):  
Small Intestine ◽  
Age Groups ◽  
Negative Relationship ◽  
Meleagris Gallopavo ◽  
Cell Types ◽  
Goblet Cells ◽  
Age Related ◽  
Pas Staining ◽  
Small Intestinal ◽  
Acid Mucins

The aim of the study was to investigate the goblet cell types and their density in the small intestine of bronze turkey (Meleagris meleagris gallopovo), by means of Alcian blue-PAS staining. Sixty birds from 10 age groups were used. In the duodenum and jejunum, goblet cells produced acid, neutral and mixed mucins, while in the jejunum - acid mucins. A negative relationship was observed between cell density and either duodenum or jejunum lengths; such a correlation was not established in the ileum.

Intestinal permeability modulation through a polyphenol-rich dietary pattern in older subjects: MaPLE project outcomes and perspectives

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Stefano Bernardi ◽  
Cristian Del Bo’ ◽  
Simone Guglielmetti ◽  
Giorgio Gargari ◽  
Antonio Cherubini ◽  
...  
Keyword(s):  
Nursing Home ◽  
Intestinal Permeability ◽  
Dietary Pattern ◽  
Vascular Function ◽  
Bacterial Load ◽  
Intestinal Microbiome ◽  
Low Grade ◽  
Microbial Ecosystem ◽  
Age Related ◽  
Older Subjects

AbstractIn recent years, research has been focusing on strategies to counteract inflammatory processes and age-related diseases(1). During ageing, a low-grade systemic inflammation is often associated to an altered intestinal permeability (IP) a condition that has been shown to promote inflammation possibly through the translocation of dietary and bacterial factors into the blood stream that activates the immune system(2).In this regard, dietary pattern and environmental factors could play a fundamental role because of their potential ability to modulate inflammation, IP and the gut microbial ecosystem (GME). Moreover, it has been hypothesized that bioactive compounds such as polyphenols may affect IP and GME(3).The MaPLE project (Microbiome mAnipulation through Polyphenols for managing gut Leakiness in the Elderly) aimed to investigate the hypothesis that a polyphenol-rich diet can improve IP condition in a target population with beneficial changes at intestine and systemic level. To this aim, a randomised, controlled, cross-over dietary intervention study (8-week polyphenol-rich diet versus 8-week control diet, separated by a wash-out period) was carried out in a group of older subjects (> 60 years) living in a well-controlled setting (i.e. nursing home). Markers related with IP, inflammation, oxidative stress, vascular function and intestinal microbial ecosystem were investigated in serum, urine and/or fecal samples. Moreover, blood bacteria DNAemia, and serum/urine metabolomics has been assessed. Moreover, a consistent nutritional evaluation of the standard menu (provided by the nursing home) and of weighed food diaries was performed, providing also data on actual polyphenol intake during the intervention. The results show there were higher levels of IP in the older subjects, and that the polyphenol-enriched diet changed the levels of serum zonulin, a marker of IP. In addition, an association between zonulin and blood bacterial load was demonstrated. Ongoing in vitro and in vivo experiments are exploring the potential effects of different polyphenols on IP and the mechanisms involved. The MaPLE project will generate new data to improve the understanding on the role of polyphenols in the modulation of intestinal microbiome and its interactions with the host.

scholarly journals Update in Small Bowel Physiology: Part 1

1990 ◽  
Vol 4 (6) ◽  
pp. 243-254
Author(s):  
RJ Fingerote ◽  
S Churnratanakul ◽  
M Keelan ◽  
K Madsen ◽  
ABR Thomson
Keyword(s):  
Small Intestine ◽  
Small Bowel ◽  
Human Disease ◽  
Intestinal Function ◽  
The Past ◽  
Recent Advances ◽  
Small Intestinal ◽  
Clinical Advances

The recent advances in clinically important diseases of the small intestine have been reviewed; however, the basis for many of these clinical advances rests with important observations on alterations in the physiology of the small intestine, as well as mechanistic observations of alterations in small intestinal function in models of human disease. In this review a summary of the past year's literature is presented which will draw attention to the considerable areas of progress in small bowel physiology which will soon be translated into an improved understanding of the pathophysiology of a variety of intestinal disorders.

scholarly journals Gastric acid inhibitor aggravates indomethacin-induced small intestinal injury via reducing Lactobacillus johnsonii

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yuji Nadatani ◽  
Toshio Watanabe ◽  
Wataru Suda ◽  
Akinobu Nakata ◽  
Yuji Matsumoto ◽  
...  
Keyword(s):  
Small Intestine ◽  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Operational Taxonomic Unit ◽  
Intestinal Injury ◽  
Intestinal Microbiome ◽  
Intestinal Damage ◽  
Small Intestinal Injury ◽  
Small Intestinal

AbstractProton pump inhibitors (PPIs) alter the composition of the intestinal microbiome, exacerbating indomethacin (IND)-induced small intestinal damage. Vonoprazan fumarate inhibits gastric acid secretion using a different mechanism from PPIs. We investigated the effects of both drugs on the intestinal microbiome and IND-induced small intestinal damage. We sought to clarify whether PPI-induced dysbiosis and worsening of the damage were due to a specific drug class effect of PPIs. Rabeprazole administration increased operational taxonomic unit numbers in the small intestines of C57BL/6 J mice, whereas the difference was not significant in the vonoprazan-treated group but exhibited a trend. Permutational multivariate analysis of variance of the unweighted UniFrac distances showed significant differences between vehicle- and vonoprazan- or rabeprazole-treated groups. L. johnsonii was the predominant microbial species, and the population ratio decreased after vonoprazan and rabeprazole administration. The vonoprazan- and rabeprazole-treated groups showed increased IND-induced damage. This high sensitivity to IND-induced damage was evaluated by transplantation with contents from the small intestine of mice treated with either vonoprazan or rabeprazole. Supplementation of L. johnsonii orally in mice treated with rabeprazole and vonoprazan prevented the increase in IND-induced small intestinal damage. In conclusion, both rabeprazole and vonoprazan aggravated NSAID-induced small intestinal injury by reducing the population of L. johnsonii in the small intestine via suppressing gastric acid secretion.

scholarly journals Endoscopic Evaluation of the Small Intestine

2002 ◽  
Vol 16 (3) ◽  
pp. 178-185 ◽  
Author(s):  
Steven J Shields ◽  
Jacques van Dam
Keyword(s):  
Small Intestine ◽  
Small Bowel ◽  
Intestinal Bleeding ◽  
Endoscopic Evaluation ◽  
Design And Development ◽  
Intestinal Pathology ◽  
Small Intestinal ◽  
Small Bowel Endoscopy

Technological achievements in the area of endoscope design and development have resulted in instruments capable of advancing beyond the reach of simple gastroscopes. Such instruments, known as enteroscopes, form the bases of small bowel endoscopy. Recent widespread use of enteroscopes have contributed significantly to the understanding of small intestinal pathology and improved the ability to diagnose and treat patients with intestinal bleeding sources.

scholarly journals Endogenous Small Intestinal Microbiome Determinants of Transient Colonization Efficiency by Bacteria from Fermented Dairy Products; A Randomized Controlled Trial

2021 ◽  
Author(s):  
Edoardo Zaccaria ◽  
Tim Klaassen ◽  
Annick M.E. Alleleyn ◽  
Jos Boekhorst ◽  
Tamara Smokvina ◽  
...  
Keyword(s):  
Small Intestine ◽  
Energy Metabolism ◽  
Intestinal Microbiota ◽  
Lactobacillus Delbrueckii ◽  
Intestinal Microbiome ◽  
Microbial Composition ◽  
Microbiome Composition ◽  
Mucosal Barrier Function ◽  
Small Intestinal ◽  
Intestine Microbiome

Abstract BackgroundThe effects of fermented food consumption on the small intestine microbiome and its role on host homeostasis are largely uncharacterized as our knowledge on intestinal microbiota relies mainly on faecal samples analysis. We investigated changes in the small intestinal microbial composition and functionality, short chain fatty acid (SCFA) profiles, and on the gastro-intestinal (GI) permeability in ileostomy subjects upon the consumption of fermented milk products.ResultsWe report the results from a randomized, cross-over, explorative study where 16ileostomy subjects underwent 3, 2-week interventions periodsin which they daily consumed either milk fermented by Lacticaseibacillus rhamnosus CNCM I-3690, or milk fermented by Streptococcus thermophilus CNCM I-1630 and Lactobacillus delbrueckii subsp. bulgaricus CNCM I-1519, or a chemically acidified milk (placebo). Weperformed metataxonomic, metatranscriptomic analysis and SCFA profiling of ileostomy effluents as well as a sugar permeability test andto investigate the microbiome impact of these interventions and their potential effect on mucosal barrier function. Consumption of the intervention products significantly impacted the small intestinal microbiome composition and functionality but did not affect the SCFA levels in ileostoma effluent, or the gastro-intestinal permeability. Theimpact on microbiome composition was highly personalized,andwe identified the poorly characterized bacterial family, Peptostreptococcaceae, to be positively associated with low abundance of the ingested bacteria. Activity profiling of the microbiota revealed that carbon- versus amino acid-derived energy metabolism of the endogenous microbiome could be responsible for the individual-specific intervention effects on the small intestine microbiome composition and function.ConclusionsThe ingested bacteria are the main drivers of the intervention effect on the small intestinal microbiota composition. Their transient abundance level is highly personalized and influenced by the energy metabolism of the ecosystem that is reflected by its microbial composition (http://www.clinicaltrials.gov, ID NCT NCT02920294).

scholarly journals Insights from evolutionarily relevant models for human ageing

2020 ◽  
Vol 375 (1811) ◽  
pp. 20190605
Author(s):  
Melissa Emery Thompson ◽  
Alexandra G. Rosati ◽  
Noah Snyder-Mackler
Keyword(s):  
Individual Characteristics ◽  
Ageing Process ◽  
Model Organisms ◽  
Small Scale ◽  
Human Populations ◽  
Ageing Population ◽  
Human Lifespan ◽  
Age Related ◽  
The Social ◽  
Human Ageing

As the world confronts the health challenges of an ageing population, there has been dramatically increased interest in the science of ageing. This research has overwhelmingly focused on age-related disease, particularly in industrialized human populations and short-lived laboratory animal models. However, it has become clear that humans and long-lived primates age differently than many typical model organisms, and that many of the diseases causing death and disability in the developed world are greatly exacerbated by modern lifestyles. As such, research on how the human ageing process evolved is vital to understanding the origins of prolonged human lifespan and factors increasing vulnerability to degenerative disease. In this issue, we highlight emerging comparative research on primates, highlighting the physical, physiological, behavioural and cognitive processes of ageing. This work comprises data and theory on non-human primates, as well as under-represented data on humans living in small-scale societies, which help elucidate how environment shapes senescence. Component papers address (i) the critical processes that comprise senescence in long-lived primates; (ii) the social, ecological or individual characteristics that predict variation in the pace of ageing; and (iii) the complicated relationship between ageing trajectories and disease outcomes. Collectively, this work provides essential comparative, evolutionary data on ageing and demonstrates its unique potential to inform our understanding of the human ageing process. This article is part of the theme issue ‘Evolution of the primate ageing process’.

Small Intestinal Perforation Secondary to Necrotizing Enteritis—An Under-Recognized Complication of Crohn’s Disease

2022 ◽  
pp. 000313482110545
Author(s):  
Carlos Theodore Huerta ◽  
Antoine J. Ribieras ◽  
Karishma Kodia ◽  
D. Dante Yeh ◽  
David Kerman ◽  
...  
Keyword(s):  
Small Intestine ◽  
Crohn’S Disease ◽  
Small Bowel ◽  
Crohn's Disease ◽  
Intestinal Perforation ◽  
Bowel Perforation ◽  
Case Fatality ◽  
Regional Enteritis ◽  
Small Bowel Perforation ◽  
Small Intestinal

Small bowel perforation is an uncommon but severe event in the natural history of Crohn’s disease with fewer than 100 cases reported. We review Crohn’s disease cases with necrotizing enteritis and share a case of a 26-year-old female who presented with a recurrent episode of small intestinal perforation. A PubMed literature review of case reports and series was conducted using keywords and combinations of “Crohn’s disease,” “small intestine perforation,” “small bowel perforation,” “free perforation,” “regional enteritis,” and “necrotizing enteritis.” Data extracted included demographic data, pre- or postoperative steroid administration, medical or surgical management, and case fatality. Nineteen reports from 1935 to 2021 qualified for inclusion. There were 43 patients: 20 males and 23 females with a mean age of 36 ± 15 years old. 75 total perforations were described: 56 ileal (74.6%), 15 jejunal (20.0%), 2 cecal (2.7%), and 1 small intestine non-specified (2.7%). 38 of 43 patients were managed surgically by primary repair (11), ostomy creation (21), or an anastomosis (11). Of 11 case fatalities, medical management alone was associated with higher mortality (5/5; 100% mortality) compared to those treated surgically (6/38; 15.8% mortality; P < .001). Patient sex, disease history, acute abdomen, and pre- or postoperative steroid use did not significantly correlate with mortality. Jejunal perforation was significantly ( P = .028) associated with event mortality while ileal was not ( P = .45). Although uncommon, necrotizing enteritis should be considered in Crohn’s patients who present with small intestinal perforation. These cases often require urgent surgical intervention and may progress to fulminant sepsis and fatality if not adequately treated.

scholarly journals Paneth cell–derived growth factors support tumorigenesis in the small intestine

2020 ◽  
Vol 4 (3) ◽  
pp. e202000934
Author(s):  
Qing Chen ◽  
Kohei Suzuki ◽  
Luis Sifuentes-Dominguez ◽  
Naoteru Miyata ◽  
Jie Song ◽  
...  
Keyword(s):  
Small Intestine ◽  
Growth Factors ◽  
Small Bowel ◽  
Genetic Model ◽  
Paneth Cell ◽  
Tumor Formation ◽  
Intestinal Epithelial ◽  
Α Subunit ◽  
Conditional Expression ◽  
Small Intestinal

Paneth cells (PCs) are small intestinal epithelial cells that secrete antimicrobial peptides and growth factors, such as Wnt ligands. Intriguingly, the context in which PC-derived Wnt secretion is relevant in vivo remains unknown as intestinal epithelial ablation of Wnt does not affect homeostatic proliferation or restitution after irradiation injury. Considering the importance of growth factors in tumor development, we explored here the role of PCs in intestinal carcinogenesis using a genetic model of PC depletion through conditional expression of diphtheria toxin-α subunit. PC depletion in ApcMin mice impaired adenoma development in the small intestine and led to decreased Wnt3 expression in small bowel adenomas. To determine if PC-derived Wnt3 was required for adenoma development, we examined tumor formation after PC-specific ablation of Wnt3. We found that this was sufficient to decrease small intestinal adenoma formation; moreover, organoids derived from these tumors displayed slower growth capacity. Overall, we report that PC-derived Wnt3 is required to sustain early tumorigenesis in the small bowel and identify a clear role for PC-derived Wnt production in intestinal pathology.

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