Varieties’ Response of Vegetable Cowpea to Mechanical Inoculation of Cowpea Aphid-Borne Mosaic Virus  in Burkina Faso

Vegetable cowpea is eaten mainly fresh, in the form of young, immature pods, tender and sweet like the common bean. However, like cowpea with seeds, vegetable cowpea experience yield losses due to the cowpea aphid-borne mosaic virus (CABMV). This study aims to improve yields through the development of vegetable cowpea varieties resistant to CABMV. The study focused on ten varieties of vegetable cowpea, carried out in a greenhouse at the Kamboinsé research station using a randomized complete block design with three replications, all inoculated with CABMV. The data collection concerned resistance parameters. Mechanical inoculation made it possible to observe various symptoms of CABMV, thus highlighting the existence of variability within the varieties tested. Strong correlations were observed between several variables. Thus, the varieties of vegetable cowpea IT85F-2089-5, UG-CP-8, IT85F-2805-5 and Telma were identified as resistant, because belonging to the low severity classes and having a low value of area under the disease progress curve. On the other hand, the varieties RW-CP-5, UG-CP-6, IT83S-911, niébé baguette grimpant possessing a high severity class were judged to be susceptible. These resistant varieties will thus be able to contribute to the improvement of production and the protection of cowpea resources in Burkina.

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Screening of Passiflora species for reaction to Cowpea aphid-borne mosaic virus reveals an immune wild species

Scientia Agricola ◽

10.1590/s0103-90162009000300018 ◽

2009 ◽

Vol 66 (3) ◽

pp. 414-418 ◽

Cited By ~ 17

Author(s):

Scheila da Conceição Maciel ◽

Daniel Hiroshi Nakano ◽

Jorge Alberto Marques Rezende ◽

Maria Lúcia Carneiro Vieira

Keyword(s):

Mosaic Virus ◽

Wild Species ◽

Virus Disease ◽

Passion Fruit ◽

Mechanical Inoculation ◽

Fruit Crops ◽

Rt Pcr ◽

Cowpea Aphid ◽

Virus Isolates

Cowpea aphid-borne mosaic virus (CABMV) is a potyvirus that causes the most serious virus disease of passion fruit crops in Brazil. It is transmitted by several species of aphids in a non-persistent, non-circulative manner. The reaction of 16 species of Passiflora to infection by mechanical inoculation with four Brazilian isolates of CABMV was evaluated under greenhouse conditions. Only P. suberosa, a wild species, was resistant to infection by all virus isolates, in two independent assays. P. suberosa grafted onto infected P. edulis f. flavicarpa did not develop symptoms; neither was the virus detected by RT-PCR in the upper leaves, suggesting that this species is immune to CABMV.

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Evaluating Sheath Blight Resistance in Rice Using Detached Tiller and Field Screening Method

Journal of Nepal Agricultural Research Council ◽

10.3126/jnarc.v1i0.15717 ◽

2016 ◽

Vol 1 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Bedanand Chaudhary

Keyword(s):

Field Experiments ◽

Agricultural Research ◽

Disease Incidence ◽

Block Design ◽

Screening Method ◽

Sheath Blight ◽

Research Station ◽

Rice Varieties ◽

Progress Curve ◽

Rice Genotypes

In present study physical resistance test on 12 rice varieties against sheath blight (ShB) caused by Rhizoctonia solani was examined. A detached tiller test was used to measure components of ShB physiological resistance at Regional Agricultural Research Station (RARS), Tarahara, Nepal. The varieties were evaluated in a randomized complete block design (RCBD) with 3 replications in year 2010-12. A sclerotium was inserted below the leaf collar of individual tillers maintained in tubes filled with water. Only the 56-day old susceptible plants were inoculated. After 7 days of inoculation, number of lesions, dead leaves, vertical sheath colonization and disease severity were measured. In 2011, field experiments were conducted with three replications to address morphological resistance in 28 rice genotypes. Disease incidence was recorded on 14, 21 and 28 days after inoculation. The number of dead leaves differed only numerically while disease variables significantly (P=0.004) varied among varieties. The other variables were highly correlated with each other. Cluster analysis of variables formed three varietal groups; Sabitri with lowest, and Jasmine-85 and Betichikon having highest values. Rice genotypes differed significantly (P=0.05) for disease incidence and area Under Disease Progress Curve (AUDPC). Tetep and IAC-165 had the lowest and highest terminal disease incidences and AUDPC, respectively. Sabitri, MTU-1010 and IR-26 recorded lower AUDPC values. Hence, Tetep and Sabitri could be used as donors in hybridization and their plant morphology could be considered as selection guide for improving ShB resistance in rice.Journal of Nepal Agricultural Research Council Vol.1 2015 pp.1-8

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Differential and comparative screening of cowpea varieties to Striga gesnerioides (Willd.) Vatke for race specific identification in Burkina Faso

African Crop Science Journal ◽

10.4314/acsj.v29i1.7 ◽

1970 ◽

Vol 29 (1) ◽

pp. 101-118

Author(s):

P. Sawadogo ◽

T.J. Ouedraogo ◽

Z. Dieni ◽

T.B.J. Batieno ◽

N. Sawadogo ◽

...

Keyword(s):

Burkina Faso ◽

Intraspecific Variability ◽

Intraspecific Diversity ◽

Research Station ◽

Striga Gesnerioides ◽

Resistant Varieties ◽

Artificial Infestation ◽

Cowpea Varieties ◽

Specific Reactions

Significant efforts have been made to develop cowpea (Vigna unguiculata (L.) Walp.) varieties resistant to Striga gesnerioides in Burkina Faso. Despite these efforts, the resistant genotypes developed still express differential responses to Striga gesnerioides in different zones of the country. This suggests existence of intraspecific variability within the parasite. The objective of this study was to assess the intraspecific variability of cowpea genotypes to Striga gesnerioides infection in Burkina Faso. Ten cowpea varieties were screened over two consecutive years, under artificial infestation with 30 ecotypes of Striga seeds at Kamboinsé research station, in a screenhouse in Burkina Faso. Cowpea varieties used included Moussa local, Komsaré and KVx404-8-1, which are susceptible to all Striga ecotypes; and varieties B301, IT93K-693-2 and IT82D-849, which are free from all Striga ecotypes infestation. Cowpea varieties Tiligré, 524B, local Gorom and Niizwè had specific reactions depending on the ecotypes. The study highlights the existence of varietal specificities according to the geographical origin of Striga gesnerioides seeds. The structuring of the intraspecific diversity showed five biotypes, of which three were clearly identified as SG1, SG5 and SG Kp races; and two biotypes could not be identified. Although this study did not allow for a clear determination of the racial affiliation of the two new biotypes, it offers the possibility of developing new strategies to control Striga by focusing on the selection of resistant varieties based on regional specificities of Striga races in each agricultural zone.

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Economic Management of Late Blight (Phytophthora infestans L.) of Potato in Eastern Tarai of Nepal

Nepal Agriculture Research Journal ◽

10.3126/narj.v6i0.3365 ◽

1970 ◽

Vol 6 ◽

pp. 57-61 ◽

Cited By ~ 3

Author(s):

Prem CP Chaurasia

Keyword(s):

Late Blight ◽

Disease Severity ◽

Field Experiments ◽

Tuber Yield ◽

Block Design ◽

Winter Season ◽

Cost Ratio ◽

Research Station ◽

Terminal Disease ◽

Progress Curve

Field experiments were carried out at Regional Agriculture Research Station, Tarahara in threeconsecutive years (1997-2000) during winter season to know the economic sprays of differentfungicides to manage late blight disease of potato. Late blight susceptible cultivar of potatoKufri Sindhuri was used for the experiment. Krinoxyl gold (metalaxyl 8% and mancozeb 64%)at the rate of 1.5 g/l, Dithane M- 45 (mancozeb 75%) @ 0.3% and Bordeaux mixture @ 10 gcopper sulfate and 10 g lime/l of water and their different combinations were tested inrandomized complete block design with three replications. Terminal disease severity was thehighest (7.9) on 0-9 scale disease rating in unsprayed check and it was significantly higher thanthose of other fungicides sprays. Plots with three sprays of Dithane M- 45 @ 0.3% recorded thelowest (2.4) terminal disease severity. Disease development was the fastest in the unsprayed plotcompared to all other treatments. Similarly apparent infection rate was the highest in unsprayedtreatment. Area Under the Disease Progress Curve was also the highest in unsprayed plot and itwas significantly higher than those of other treatments. Tuber yield was the lowest (3.93 kg/4.5m2 plot in unsprayed and the highest (6.08 kg), in the plot receiving four sprays of Dithane M-45@ 0.3 % and this was at par with the tuber yield of plots receiving two and three sprays ofDithane M-45. Benefit cost ratio was the highest (1.54) in case of two sprays of Dithane M-45@ 0.3% at 10 days intervals. All others fungicide spays were uneconomical under Eastern Taraicondition.Key words: Eastern Tarai; economic spray; Dithane M-45; late blight management; potatoDOI: 10.3126/narj.v6i0.3365Nepal Agriculture Research Journal Vol.6 2005 pp.57-61

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Selection and validation of marker set for selection of resistant varieties of cowpea to Cowpea Aphid-borne Mosaic Virus (CABMV) in Burkina Faso

Journal of Applied Biosciences ◽

10.4314/jab.v128i1.8 ◽

2019 ◽

Vol 128 (1) ◽

pp. 12953

Author(s):

Antoine Barro ◽

Zakaria Kiebre ◽

Pauline Bationo-Kando ◽

Jean Baptiste De La Salle Tignegre ◽

Téyouré Benoit Joseph Batieno ◽

...

Keyword(s):

Burkina Faso ◽

Mosaic Virus ◽

Resistant Varieties ◽

Cowpea Aphid ◽

Selection Of

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Management of Botrytis Gray Mold (Botrytis cinerea Pers. Ex. Fr.) of Chickpea at Tarahara, Nepal

Nepal Agriculture Research Journal ◽

10.3126/narj.v4i0.4867 ◽

1970 ◽

pp. 37-41 ◽

Cited By ~ 1

Author(s):

Prem CP Chaurasia ◽

Sharada Joshi

Keyword(s):

Grain Yield ◽

Botrytis Cinerea ◽

Block Design ◽

Trichoderma Viride ◽

Correlation Coefficients ◽

Gray Mold ◽

Research Station ◽

Research Journal ◽

Yield Production ◽

Progress Curve

An experiment was conducted in randomized complete block design with six treatments and four replications at loamy soil of Regional Agriculture Research Station, Tarahara, Nepal during 1997/98 and1998/99 seasons to find out the effect of bioagent Trichoderma viride as compared to fungicide Bavistin (carbendazim) for management of Botrytis gray mold (Botrytis cinerea) of chickpea (Cicer arietinum). Treatments were use of water spray (check), three sprays of T. viride (107-108 spores/ml of water), three sprays of Bavistin @ 0.2%, three sprays of Bavistin @ 0.1% + T. viride, two sprays of Bavistin @ 0.2% and two sprays of T. viride. The area under the disease progress curve (AUDPC) was the least (193.6) with three sprays of Bavistin (0.2%) followed by two sprays of Bavistin (0.2%) and three sprays of Bavistin + T. viride (216.9). The highest mean grain yield of 267.3 kg ha-1 was produced by three sprays of Bavistin + T. viride followed by three sprays of Bavistin. Three sprays of T. viride was inferior to three and two sprays of Bavistin but it was at par with two sprays of T. viride with respect to grain yield production and reduction of Botrytis gray mold severity. When AUDPC was regressed against grain yield, a negative correlation was obtained between the disease and the grain yield. The correlation coefficients for the disease and yields during 1997/98 and 1998/99 respectively were -0.583, and -0.490.Key words: Botrytis gray mold; Chickpea; TrichodermaDOI: http://dx.doi.org/10.3126/narj.v4i0.4867Nepal Agriculture Research Journal Vol. 4&5, 2001/2002Page: 37-41Uploaded date: 9 June, 2011

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Cowpea aphid-borne mosaic virus (CABMV) in Passiflora by qPCR analysis reveals asymptomatic plants with viruses and new species with immunity

10.21203/rs.3.rs-234183/v1 ◽

2021 ◽

Author(s):

Zanon Santana Gonçalves ◽

Onildo Nunes de Jesus ◽

Lucas Kennedy Silva Lima ◽

Ronan Xavier Corrêa

Keyword(s):

New Species ◽

Virus Infection ◽

Mosaic Virus ◽

Disease Index ◽

Passion Fruit ◽

Rt Pcr ◽

Qpcr Analysis ◽

Resistant Varieties ◽

Cowpea Aphid ◽

Temporal Progress

Abstract The passion fruit woodiness disease (Cowpea aphid-borne mosaic virus – CABMV) causes socioeconomic problems for Brazilian passion fruit crop. Understanding the temporal progress of the disease and identifying resistance sources to CABMV are essential steps to develop resistant varieties. The objective of the study was to evaluate temporal progress of passion fruit woodiness disease, identify Passiflora genotypes with CABMV resistance and to detect virus infection in asymptomatic plants by qPCR. The experiment was conducted in a greenhouse using 128 genotypes belonging to 12 species and three hybrids (inter and intraspecific) of Passiflora evaluated in five periods after inoculation. The symptoms severity was quantified from the disease index (DI%). The CABMV infection in symptomatic plants was confirmed by RT-PCR and in asymptomatic plants by qPCR. Progress rates and disease severity were lower in the species P. cincinnata, P. gibertii, P. miersii e P. mucronata compared to P. edulis, P. alata, Passiflora sp. and hybrids. Of the evaluated genotypes, 20.31% were resistant, with emphasis on the accessions of P. suberosa, P. malacophylla, P. setacea, P. pohlii e P. bahiensis that did not show symptoms of virus. The absence of symptoms does not imply immunity of plants to the virus, as the qPCR analysis confirmed infection by the virus in asymptomatic plants of P. cincinnata, P. gibertii, P. miersii, P. mucronata, P. setacea, P. malacophylla e P. suberosa. Even after four inoculations, the virus was not detected by qPCR in the upper leaves in plants of P. pohlii and P. bahiensis indicating that these species are immune to CABMV.

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Effect of Integration of Fertilizers and FYM on Productivity and Soil Health of Rainfed Potato

Journal of AgriSearch ◽

10.21921/jas.v4i1.7414 ◽

2017 ◽

Vol 4 (1) ◽

Author(s):

S. K. YADAV ◽

A. K. SRIVASTAVA ◽

T. K. BAG

Keyword(s):

Nutrient Management ◽

Block Design ◽

Soil Health ◽

Growth And Yield ◽

Recommended Dose ◽

Research Station ◽

Yield Attributes ◽

Hill Region ◽

North Eastern ◽

Synthetic Fertilizers

A field trial was conducted during two consecutive summer seasons of2012 and 2013 at ICAR-Central Potato Research Station, Shillong, Meghalaya to evaluate the integration of nutrient sources on productivity and soil health under rainfed potato cultivation in north eastern hill region of India. There were six treatments of integrated nutrient management viz., 100% Recommended dose of fertilizers,75% RDF through synthetic fertilizers and 25% recommended dose of nitrogen (RDN) through FYM, 50% RDF and 50% RDN through FYM, 25% RDF and 75% RDN through FYM, 100% RDN through FYM and control (no application of any sources of nutrients). The experiment was laid out in randomized block design with four replications. Nutrient management practices showed the significant improvement on growth and yield attributes of potato over control plot. Highest productivity of potato tubers (t/ha) was noticed with application of 75% RDF through synthetic fertilizers along with 25% RDN through FYM. Similarly, the maximum net return was associated with application of 75% RDF and 25% RDN through FYM under investigation. Application of 75% Recommended dose of nutrients through synthetic fertilizers in combination with 25% Recommended dose of nitrogen through FYM was more profitable for sustainable production of potato in the north eastern hill region of India.

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Nitrogen Requirement of Potato for Eastern Indo-Gangatic Plain of India

Journal of AgriSearch ◽

10.21921/jas.v7i03.18686 ◽

2020 ◽

Vol 7 (03) ◽

Author(s):

S. K. YADAV ◽

R. K. SINGH ◽

V. K. DUA ◽

S SINGH ◽

SARALA YADAV ◽

...

Keyword(s):

Tuber Yield ◽

Potato Crop ◽

Block Design ◽

Nitrogen Level ◽

Cost Ratio ◽

Research Station ◽

Net Benefit ◽

Marketable Tuber ◽

Benefit Cost Ratio ◽

Significant Difference

A field experiment was carried out during 2015-16 and 2016-17 at ICAR-Central Potato Research Station, Patna, Bihar, in randomized block design with four replications with objective to quantity the optimum requirement of nitrogen to potato crop. Significantly the highest plant height was recorded with nitrogen level of 300 kg/ha. Distributions of the smallest size of tuber yield of potato were decreasing with increasing the level of nitrogen. There was about 26.0% more yield of the smallest size tuber of potato was recorded with zero nitrogen as compare to the highest level of nitrogen i.e. 300 kg/ha. Increasing the nitrogen over the level of 150 to 225 kg per hectare, increasing the yield of tuber gradually slower rate than nitrogen level from 0 to 150 kg/ha. Total and marketable tuber yield of potato were increasing significantly with increasing level of nitrogen up to 150 kg/ha. There was no significant difference in marketable tuber yield was found for nitrogen level between 150 and 225 kg/ha. Highest (1.66) net benefit cost ratio was also recorded with level of nitrogen @ 150 kg/ha in potato. Hence, application of nitrogen @ 150 kg/ha was found statistically and economically more beneficial for potato cultivation in Eastern Indo-Gangatic plain of India.

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Seed Yam Production Using High-Quality Minitubers Derived from Plants Established with Vine Cuttings

Agronomy ◽

10.3390/agronomy11050978 ◽

2021 ◽

Vol 11 (5) ◽

pp. 978

Author(s):

Beatrice Aighewi ◽

Norbert Maroya ◽

Lava Kumar ◽

Morufat Balogun ◽

Daniel Aihebhoria ◽

...

Keyword(s):

Block Design ◽

Research Station ◽

International Institute ◽

Tropical Agriculture ◽

Single Node ◽

Cropping Season ◽

Randomized Complete Block Design ◽

The World ◽

Cropping Seasons ◽

Set Up

Yam (Dioscorea spp.) is a valuable food security crop in West Africa, where 92% of the world production occurs. The availability of quality seed tubers for increased productivity is a major challenge. In this study, minitubers weighing 1, 3, and 5 g produced from virus-free single-node vine cuttings of two improved yam varieties (Asiedu and Kpamyo) growing in an aeroponics system were assessed for suitability in seed production at a population of 100,000 plants ha−1. A 3 × 2 factorial experiment with randomized complete block design and three replications was set up during the cropping seasons of 2017 to 2019 at the International Institute of Tropical Agriculture Research Station in Kubwa, Abuja, Nigeria. Results showed field establishments of 87%–97.8%. Yields differed with minituber size, variety, and cropping season; the highest was 31.2 t ha−1 in 2019 and the lowest, 10 t ha−1 in 2018 from 5 and 1 g Kpamyo minitubers, respectively. The estimated number of tubers produced per hectare by 1, 3, and 5 g minitubers was 101,296, 112,592, and 130,555, with mean weights per stand of 159.2, 187.3, and 249.4 g, respectively. We recommend using less than 6 g minitubers for seed yam production due to their high multiplication rates.

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