scholarly journals Produção de enzimas extracelulares por Ceratocystis spp.

2014 ◽  
Vol 40 (4) ◽  
pp. 371-374 ◽  
Author(s):  
Ana Carolina Firmino ◽  
Edson Luiz Furtado

O gênero Ceratocystis contempla diversas espécies distribuídas em vários lugares do mundo. No Brasil ocorrem relatos da existência de três espécies, sendo elas: C. cacaofunesta, C. paradoxa e C. fimbriata, sendo esta última relacionada a doença em culturas de importância econômica. O trabalho objetivou verificar, em meios de cultura específicos, a produção das enzimas extracelulares amilase, lipase, celulase, protease, lacase e lignina peroxidase e pectiliase (pectato-liase) por isolados de Ceratocystis sp. Foram usados 41 isolados: 3 de mangueira (Mangifera indica), 19 de eucalipto (Eucalyptus spp.), 15 de cacaueiro (Theobroma cacao), 2 de Teca (Tectona grandis) e 2 de atemóia (Annona sp.). As colônias foram incubadas no escuro a 25ºC , com exceção do meio para detecção de pectinase que foi incubado sob fotoperíodo alternado. O delineamento experimental foi inteiramente casualizado com 4 repetições. A partir dos meios específicos foi possível detectar a produção de amilase, lacase e protease quase na totalidade dos isolados Ceratocystis sp. testados. Não foi observada a produção de celulase, lípase e pectatoliase. A produção da enzima lignina peroxidade foi detectada em pouca quantidade e em somente alguns isolados do fungo. Este perfil enzimático obtido da população do fungo pode auxiliar em futuros estudos relacionados com a caracterização deste.

2006 ◽  
Vol 30 (6) ◽  
pp. 1099-1105 ◽  
Author(s):  
Fernando Luíz de Oliveira Corrêa ◽  
José Darlan Ramos ◽  
Antônio Carlos da Gama-Rodrigues ◽  
Manfred Willer Müller

Objetivou-se com este trabalho avaliar a produção de serapilheira em diferentes coberturas frutíferas e florestais componentes de um sistema agroflorestal multiestratificado localizado no município de Ouro Preto do Oeste, RO, Brasil. As espécies avaliadas foram: mangueira (Mangifera indica L.), fruta-pão (Artocarpus altilis (Parkinson) Fosberg), cupuaçuzeiro (Theobroma grandiflorum Schum), abacateiro (Persea americana Mill.), cacaueiro (Theobroma cacao L.) sombreado com gliricídia (Gliricidia sepium (Jacq.) Walp.) e as espécies florestais, a bandarra (Schizolobium amazonicum Huber ex Ducke) e a teca (Tectona grandis L.f.). Como referência foi utilizada uma área de vegetação natural (capoeira), de 8 anos de idade. A deposição anual de serapilheira entre o período de outubro de 2002 e setembro de 2003 foi de 13,38 t ha-1 para a vegetação natural, 4,02 t ha-1 para bandarra, 3,43 t ha-1 para gliricídia, 2,86 t ha-1para abacateiro, 2,54 t ha-1 para fruta-pão, 1,40 t ha-1 para o cupuaçuzeiro, 1,16 t ha-1 para o cacaueiro, 1,12 t ha-1 para mangueira e 1,07 t ha-1 para a teca. A vegetação natural, a bandarra e o cacaueiro apresentaram uma maior deposição no período seco (verão), enquanto que a fruta-pão, cupuaçuzeiro, mangueira, teca, gliricídia e abacateiro as maiores deposições foram no período chuvoso (inverno). Por outro lado a serapilheira acumulada sobre o solo foi de 14,61 t ha-1 na mangueira, 12,8 t ha-1 na bandarra, 12,73 t ha-1 na vegetação natural, 12,04 t ha-1, no abacateiro, 9,87 t ha-1 no cacaueiro/gliricídia, 9,88 t ha-1 no cupuaçuzeiro, 9,05 t ha-1 na fruta-pão e 7,39 t ha-1 na teca.


2005 ◽  
Vol 30 (5) ◽  
pp. 543-545 ◽  
Author(s):  
Francisco A Ferreira ◽  
Luis A Maffia ◽  
Evander A Ferreira

Desenvolveu-se uma técnica de detecção rápida de Ceratocystis fimbriata em lenho de eucalipto (Eucalyptus spp.) infetado, visualizando-se clamidósporos (aleuroconídios) ao microscópio ótico comum, em vasos do xilema, medula e raios medulares, a partir de cortes histopatológicos à mão livre, feitos com lâmina de barbear, ao microscópio estereoscópico. O tempo médio gasto para a detecção do patógeno, do corte histopatológico tangencial à total visualização dos clamidósporos ao microscópio ótico comum, foi de 3,5 min e bem menos utilizando-se corte longitudinal passando pela medula, contra, no mínimo, quatro a cinco dias, usando-se outras técnicas como o isolamento em BDA, deposição de fragmentos de lenho doente entre fatias de cenoura usadas como isca, ou pedaços de lenhos doentes deixados em câmara úmida. Essa técnica histopatológica é também viável para a detecção do patógeno em outros hospedeiros lenhosos e, inclusive, para a detecção de hifas de Lasiodiplodia theobromae, mesmo quando esses dois fungos estavam num mesmo tecido, como na doença-complexo seca de mangueira investigada no Sultanato de Omã. Além de eucalipto, mangueira (Mangifera indica) e cacaueiro (Theobroma cacao) é provável que essa técnica possa ser estendida para outros hospedeiros lenhosos de C. fimbriata.


Author(s):  

Abstract A new distribution map is provided for Orgyia postica (Walker) Lepidoptera: Lymantriidae Attacks cocoa (Theobroma cacao), tea (Camellia sinensis), coffee (Coffea spp.), soyabean (Glycine max), rubber (Hevea brasiliensis), mango (Mangifera indica), grapevine (Vitis vinifera) and other crops. Information is given on the geographical distribution in ASIA, Bangladesh, Brunei Darussalam, China, Fujian, Guangdong, Guangxi, Hainan, Hong Kong, Yunnan, India, Kerala, Nagaland, Sikkim, Tamil Nadu, Uttar Pradesh, Indonesia, Java, Kalimantan, Sulawesi, Sumatra, Japan, Ryukyu Archipelago, Laos, Malaysia, Myanmar, Philippines, Sri Lanka, Taiwan, Thailand, Vietnam, OCEANIA, Papua New Guinea.


2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Nova Annisa

Penelitian ini bertujuan untuk mengetahui jenis pohon yang sesuai untuk rain garden, jumlah stomata, proporsi penutupan kanopi dan kandungan air dalam daun dari beberapa jenis pohon teduhan, serta hubungan antara jumlah stomata daun dengan luas daun, jika terdapat faktor proporsi kandungan air yang diduga mempengaruhi akan dikendalikan. Data yang diperoleh di analisis  proporsi kandungan air dalam daun, proporsi penutupan kanopi, dan jumlah stomata dengan menggunakan analisis deskriptif. Untuk mengetahui hubungan antara luas daun, proporsi penutupan kanopi dan kandungan air dalam daun pada pohon teduhan digunakan analisis korelasi parsial dengan bantuan SPSS versi 17. Berdasarkan hasil penelitian terhadap kerapatan stomata dalam ukuran  1cm², diperolehlah 10 jenis pohon teduhan tertinggi seperti Psidium guajava (jambu biji), Mangifera casturi (kasturi), Hevea brasiliensis  (karet), Pithecellobium lobatum (jengkol), Mangifera indica (mangga), Theobroma cacao (coklat), Persea americana (alpukat), Eugenia aquea (jambu air), Gnetum gnemon (melinjo), dan Arthocarpus heterophyllus (nangka). Kerapatan stomata daun tidak berhubungan terhadap luas daun pada pohon teduhan. Kata kunci: pohon teduhan rain garden, stomata. This study aims to determine the type of tree suitable for rain garden, number of stomata, the proportion of canopy closure and water content in the leaves of several types of shade trees, and the relationship between the number of leaf stomata to leaf area, if there is a proportion factor thought to affect the water content will be controlled. Data obtained in the analysis of the proportion of water content in the leaves, the proportion of canopy closure, the location of the tree, and the number of stomata by using descriptive analysis. To determine the relationship between leaf area, the proportion of canopy closure and water content in the leaves of the shade trees used partial correlation analysis with SPSS version 17. Based on the results of a study of the density of stomata in the size of 1 cm², found 10 species of trees shade the best such as Psidium guajava (guava), Mangifera casturi (kasturi), Hevea brasiliensis (rubber), Pithecellobium lobatum (jengkol), Mangifera indica (mango), Theobroma cacao (brown), Persea americana (avocado), Eugenia aquea (rose apple), Gnetum gnemon (melinjo), and Arthocarpus heterophyllus (jackfruit). The density of leaf stomata are not related to the broad leaves of the shade tree. Keywords: rain garden, shade tree, stomata.


2019 ◽  
Vol 11 (8) ◽  
pp. 90
Author(s):  
Sandrine Jueya ◽  
Ruth Mony ◽  
Champlain Djieto-Lordon

The present study was carried out in order to identify the ant fauna and her potential role as auxiliary of an agricultural system based on cocoa trees leeched by Loranthaceae, in Nkolbisson neighborhood in Yaoundé (Cameroon). The work was conducted from May to September 2015. An inventory of all woody species and the associated ant fauna was carried out beforehand, followed by an inventory of the ant fauna associated couples Theobroma cacao/Loranthaceae during periods of non-flowering, flowering and fruiting Loranthaceae. We inventoried a total of 187 plants of which 44 (23.53%) were parasitized by flowering-shrub epiphytes (Loranthaceae). Theobroma cacao (cocoa) appeared to be the most abundant species (122 plants) followed by Dacryodes edulis (16 safou trees) and Mangifera indica (15 mango trees). Three species of Loranthaceae were identified: Tapinanthus preussii (Engler) Van Tieghem and Phragmanthera capitata (Sprengel) S. Balle which both attack Theobroma cacao (cocoa tree) and Tapinanthus bangwensis (Engl.et. Krause) Denser which attacks mainly forest type trees. Five sub-families of ants (Pseudomyrmicinae, Dolychoderinae, Ponerinae, Formicinae and Myrmicinae) divided into 16 genera and 35 species were recorded throughout the agrosystem. Tetramorium acculeatum has emerged as the most dominant ant species on both T. preussii and P. capitata. Monitoring of the ant foraging activity during flowering and fruiting Loranthaceae shows they participate in the flowers fall.


2020 ◽  
Vol 45 (5) ◽  
Author(s):  
V. N. Okafor ◽  
U. W. Okafor ◽  
R. I. Anyalebechi ◽  
M. C. Obiadi ◽  
J. N. Obiefuna ◽  
...  

Physical and flame characteristics of fifteen tree species in South-East Nigeria were investigated. The tree species Daniellia oliveri, Anacadium occidentale, Vitex doniana, Lonchocarpus griffonianus, Gmelina arborea, Nauclea latifolia, Tectona grandis, Mangifera indica, Delonix regia, Newbouldia laevis, Azadirachta indica, Dialium guineense, Terminalia superba, Manilkara obovata and Irvingia gabonensis were identified and named by taxonomists. The aim was to investigate the effects of oven and sun dried densities of timbers on major flame characteristics (ignition time, flame propagation rate, after glow time, flame duration and ash formation) and to establish timber density relationship with the flame properties. Physical properties (density and moisture content) and flame characteristics of all the timbers were carried out using their respective standard methods. Oven and sun dried densities of timbers ranged from 0.39 to 0.97 and 0.45 to 1.16 g/cm3 respectively. Moisture content of oven and sun dried timbers ranged from 8.02 to 11.56% and 8.15 to 11.65 % respectively. The range of values for ignition time were (3.00-10.00) seconds for oven dried timbers and (5.00-10.00) seconds for sun dried timbers. Flame propagation rate ranged between 0.15 and 0.27 cm/s and from 0.09 to 0.25 cm/s for oven and sun dried timbers respectively. The values for flame duration ranged from 12.00 to 56.00 seconds for oven dried timbers and 7.00 to 45.00 seconds for sun dried timbers. Afterglow times of oven and sun dried timbers ranged from 70.00 to 267.00 and 19.00 to 188.00 seconds respectively. Ash formation values ranged from 0.19 to 2.38 and 0.25 to 2.46 % for oven and sun dried timbers respectively. Combustion/pyrolysis pattern of oven and sun dried timbers on exposure to fire was similar. Density related to flame propagation rate, although, for the denser hard woods above 0.60g/cm3 and 0.70g/cm3 for oven and sun dried timbers respectively, this dependence was less straight forward. The flame characteristics of the oven dried timbers compared favourably with sun dried timbers and showed that ignition time, flame duration, afterglow time and ash formation have no clear relationship with timber density.


2020 ◽  
Vol 7 (2) ◽  
pp. 77-82
Author(s):  
Dominggus De Lima ◽  
J. S. A. Lamerkabel ◽  
Inggrid Welerubun

The aim of this research was to determine the kinds of pollen producer plants, its population density and the number of cells contained bee bread (pollen) at bee nest. The observed variables were kinds of plants in general and its density, pollen producer plants, number of cells contained bee breed (pollen). Method used was survey techniques which conducted in four bee farms. The farms were naturally rich of some flowering plants such as Cambodia, asoka, bougainville; and some horticulture plants such as coconut Cocos mufifera), nutmeg (Myristisca frogranas houtt), palm (Arenga pinnata) ,cacao (Theobroma cacao), clove (Syzygiumaromatioum); some fruits plants such as guava(Psidium guajava), nut guava(Arnacidium occidentale), ambutan(Nephelium lappaceum), mango(mangifera indica), banana(Musa paradisiacal), langsat(Lansium domesticium). From the four bee farms chosen, there was revealed that Dusun Kawatu has the highest plant density, followed by respectively Negeri Hatusua, Negeri Waehatu and Negeri Kamarian. Also, Dusun Kawatu has more pollen producer plant varieties in comparison to the other three Negeri/ Dusun(s). However, bee farm in Negeri Kamarian has more number of cell contained pollen than other farms; followed respectively by bee farms in Negeri hatusua, Dusun Kawatu and Negeri Waehatu.


2021 ◽  
Vol 2 (1) ◽  
pp. 28-32
Author(s):  
Yulita Iryani Mamulak ◽  
Chatarina Gradict Semiun

Soba Village, West Amarasi Subdistrict, Kupang Regency is located in East Nusa Tenggara Province with abundant natural resources. One of the natural resources  is  a natural spring with  abundant diversity of riparian vegetation. The diversity of riparian vegetation around water sources plays an important role in water quality. This study aimed to identify the diversity of riparian vegetation around springs and to determine water quality. The method used was plot installation, identification of riparian vegetation types, measurement of environmental factors and water quality testing. The results showed that there were 12 species of tree vegetation with a total of 74 individuals, the most common tree species found was Canangan odorata with a relative abundance of 24.32%, followed by Cocos nucifera 18.92%, and Areca sp. 16.22%. Meanwhile, the least species found were Tectona grandis, Mangifera indica, Artocarpus altilis, and Syzygium aqueum with relative species abundance of 1.35%. The results of quantitative analysis of the relative frequency found tree species Areca sp. and Cocos nucifera had the highest value, namely 19.05%. Meanwhile, the highest relative dominance value was found in thespecies Artocarpus altilis. The results of water quality testing with 3 parameters namely physical, chemical and bacteriological showed a COD value of 85.03 mg / L, a total colifrorm of 45 and a total fecal colliform  of 230


2021 ◽  
Vol 50 (7) ◽  
pp. 1901-1911
Author(s):  
Norhelaliah Isa ◽  
Noor-Soffalina Sofian-Seng ◽  
Wan Aida Wan Mustapha

Buah mangga boleh dimakan segar atau diproses menjadi jus dan jeruk tetapi hanya isi digunakan dan bijinya dibuang. Kajian terdahulu mendapati bahawa biji mangga mempunyai lemak seperti lemak koko. Objektif kajian ini adalah untuk menentukan komposisi pemakanan dalam sisa biji jeruk mangga (SBJr) dan sisa biji jus mangga (SBJs); jumlah lemak antara SBJr dan SBJs melalui pengekstrakan bendalir lampau-genting (SFE); serta komposisi asid lemak dan takat lebur gelincir (SMP) terhadap lemak koko komersil (LK). Analisis proksimat menunjukkan bahawa SBJr dan SBJs mengandungi karbohidrat, lemak, protein, abu dan kelembapan. Pengekstrakan lemak SBJr dan SBJs menggunakan SFE (suhu 72 °C; tekanan 42.4 Mpa; 60 min; 4 mLmin-1 CO₂) menunjukkan lemak SBJr lebih tinggi(p<0.05) (6.4%) berbanding SBJs (3.37%). Lima jenis asid lemak ditemui dalam SBJr, SBJs dan LK iaitu asid palmitik, stearik, oleik, linoleik dan linolenik dengan kandungan (%) yang berbeza(p<0.05) bagi semua sampel. SMP bagi lemak SBJr (34.2 °C) lebih tinggi (p<0.5) diikuti oleh SBJs (32.17 °C) dan LK (30.27 °C). Walaupun ciri-ciri kimia lemak SBJr dan SBJs berbeza dengan LK, tetapi kedua-duanya berpotensi sebagai pengganti LK yang lebih baik kerana mengandungi asid lemak tak tepu yang lebih tinggi(p<0.05) dan asid lemak tepu yang lebih rendah(p<0.05) daripada LK. Penggunaan SBJr lebih praktikal kerana kandungan lemaknya tinggi dan SMP lemak ini sesuai dengan keadaan cuaca tropika.


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