scholarly journals Physicochemical Properties, Cytocompatibility and Antibiofilm Activity of a New Calcium Silicate Sealer

2021 ◽  
Vol 32 (4) ◽  
pp. 8-18
Author(s):  
Cristiane Lopes Zordan-Bronzel ◽  
Mario Tanomaru-Filho ◽  
Fernanda Ferrari Esteves Torres ◽  
Gisselle Moraima Chávez-Andrade ◽  
Elisandra Marcia Rodrigues ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Calcium Silicate ◽  
Setting Time ◽  
Neutral Red ◽  
Antibiofilm Activity ◽  
Micro Ct ◽  
Volumetric Change ◽  
Crystal Violet Assay ◽  
Ah Plus ◽  
Proper Setting

Abstract The aim of this study was to evaluate the physicochemical properties, cytocompatibility and antibiofilm activity of a new calcium silicate-based endodontic sealer, Sealer Plus BC (MK Life, Brazil), in comparison with TotalFill BC Sealer (FKG Dentaire SA, Switzerland) and AH Plus (Dentsply, Germany). Setting time and flow were evaluated based on ISO 6876 standard. The pH was evaluated after different periods, and radiopacity by radiographic analysis (mmAl). Solubility (% mass loss) and volumetric change (by micro-CT) were assessed after 30 days of immersion in distilled water. Cytocompatibility was assessed by methyltetrazolium (MTT) and neutral red (NR) assays, after exposure of Saos-2 cells to the sealer extract for 24 h. An additional analysis was performed by using MTT assay after 1, 3 and 7 days of exposure of Saos-2 to the sealers 1:8 dilution extracts. Antibiofilm activity against Enterococcus faecalis and/or Candida albicans was evaluated by crystal violet assay and modified direct contact test. The physicochemical properties were analyzed using ANOVA/Tukey tests; MTT and NR data were analyzed by ANOVA and Bonferroni tests; the antimicrobial tests were analyzed by Kruskal-Wallis and Dunn tests (α=0.05). Sealer Plus BC had proper setting time, radiopacity, flow and alkalization capacity. Sealer Plus BC was significantly more soluble than AH Plus (p<0.05) and presented volumetric change similar to AH Plus and TotalFill BC (p>0.05). Sealer Plus BC presented antibiofilm activity and no cytotoxic effect. In conclusion, although Sealer Plus BC had higher solubility, this sealer showed proper physicochemical properties, cytocompatibility, and antibiofilm activity.

scholarly journals Evaluation of Physicochemical Properties of New Calcium Silicate-Based Sealer

2018 ◽  
Vol 29 (6) ◽  
pp. 536-540 ◽  
Author(s):  
Aline Teixeira Mendes ◽  
Paula Barcellos da Silva ◽  
Bruna Barcelos Só ◽  
Lina Naomi Hashizume ◽  
Rodrigo Ricci Vivan ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Calcium Ion ◽  
Calcium Silicate ◽  
Calcium Release ◽  
Setting Time ◽  
Porto Alegre ◽  
Ion Release ◽  
Ph Values ◽  
Time Flow ◽  
Ah Plus

Abstract This study aimed to evaluate the physicochemical properties of a calcium silicate-based sealer (Sealer Plus BC; MK Life, Porto Alegre, Brazil) compared with an epoxy-resin sealer (AH Plus; Dentsply DeTrey GmbH, Konstanz, Germany). Initial and final setting time was assessed based on ISO 6876:2012 and ASTM C266:03. Calcium ion release and pH were evaluated by filling polyethylene tubes with sealers and then immersing them in 10 mL of deionized water. Following experimental periods of 1, 24, 72 and 168 hours, the samples were measured regarding pH and calcium ion release with a pH meter and a colorimetric spectrophotometer, respectively. The flow was examined based on ISO 6876:2012. Rings of 10 mm in diameter with 1 mm thickness were prepared to analyze the radiopacity (ISO 6876:2012 and ADA n.57) and solubility (ISO 6876:2012). The data were analyzed by variance analysis, Student-T and Tukey tests (p<0.05). The calcium ion release and pH values were significantly higher for the Sealer Plus BC compared with the AH Plus (p<0.05). Lower setting time, flow and radiopacity were observed for the bioceramic sealer than for AH Plus (p<0.05). Sealer Plus BC exhibited higher solubility compared with AH Plus (p<0.05). Sealer Plus BC showed physicochemical properties as setting time, pH, calcium release, flow, and radiopacity following the required standards, but higher solubility than the minimum values required by ISO 6876:2012.

scholarly journals Physicochemical Properties and Bioactive Potential of a New Epoxy Resin-based Root Canal Sealer

2019 ◽  
Vol 30 (6) ◽  
pp. 563-568 ◽  
Author(s):  
Mario Tanomaru-Filho ◽  
Maiby Cristine Prado ◽  
Fernanda Ferrari Esteves Torres ◽  
Raqueli Viapiana ◽  
Mariana Mena Barreto Pivoto-João ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Water Solubility ◽  
Setting Time ◽  
Volumetric Change ◽  
Time Flow ◽  
Before And After ◽  
Ah Plus ◽  
The Difference ◽  
Bioactive Potential ◽  
Low Solubility

Abstract The aim of this study was to evaluate physicochemical properties and bioactive potential of Sealer Plus, in comparison with MTA Fillapex, Sealapex and AH Plus. Setting time, flow, and radiopacity were evaluated based on ISO 6876 Standard. Flow was also assessed in area (mm²). The solubility and volumetric change of the sealers were evaluated after 7 and 30 days of immersion in distilled water. Solubility was evaluated by the difference in mass of materials before and after immersion. Volumetric change was evaluated by using microcomputed tomography (micro-CT). The bioactive potential was observed by Scanning Electron Microscopy (SEM) after immersion in PBS. Data were compared using ANOVA and Tukey tests (α=0.05). Sealer Plus presented the shortest setting time (196 min.) and Sealapex the longest (912 min.) (p<0.05). AH Plus showed the highest radiopacity (9.5 mm Al) and MTA Fillapex the lowest (2.7 mm Al) (p<0.05). All the sealers presented flow in accordance with ISO 6876/2012 (>17 mm). Sealer Plus showed low solubility and volumetric change (<1%), and MTA Fillapex showed the highest solubility (>25%), and volumetric change (>4%) after all time intervals (p<0.05). MTA Fillapex was the only sealer that showed bioactive potential. In conclusion, Sealer Plus presented proper physicochemical properties. However, this sealer did not present a bioactive potential.

scholarly journals Influence of Powder-to-Gel Ratio on Physicochemical Properties of a Calcium Silicate Sealer

2020 ◽  
pp. 337-345
Author(s):  
Fernanda F. E. Torres ◽  
Patricia Perinoto ◽  
Roberta Bosso-Martelo ◽  
Gisselle M. Chávez-Andrade ◽  
Juliane M. Guerreiro-Tanomaru ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Calcium Silicate ◽  
Setting Time ◽  
Repair Material ◽  
Alkaline Ph ◽  
Root Canal Sealer ◽  
Work Time ◽  
Lower Solubility ◽  
Low Solubility ◽  
One Way Anova

Differences in liquid-to-powder ratio can affect the properties of calcium silicate-based materials. This study assessed the influence of powder-to-gel ratio on physicochemical properties of NeoMTA Plus. Setting time (minutes), flow (mm and mm²), pH (at different periods), radiopacity (mm Al) and solubility (% mass loss) were evaluated using the consistencies for root repair material (NMTAP-RP; 3 scoops of powder to 2 drops of gel) and root canal sealer (NMTAP-SE; 3 scoops of powder to 3 drops of gel), in comparison to Biodentine cement (BIO) and TotalFill BC sealer (TFBC). Statistical analysis was performed using one-way ANOVA and Tukey tests (α=0.05). BIO had the shortest setting time, followed by NMTAP-RP and NMTAP-SE. TFBC showed the highest setting time and radiopacity. BIO, NMTAP-RP, and NMTAP-SE had similar radiopacity. All materials promoted an alkaline pH. NMTAP-RP/SE presented lower solubility than BIO and TFBC. Regarding the flow, TFBC had the highest values, followed by NMTAP-SE, and NMTAP-RP. BIO had the lowest flow. In conclusion, NMTAP in both powder-to-gel ratios showed high pH and low solubility. The increase in the powder ratio decreased the setting time and flow. These findings are important regarding the proper consistency and work time to clinical application.

scholarly journals Ytterbium Oxide as Radiopacifier of Calcium Silicate-Based Cements. Physicochemical and Biological Properties

2018 ◽  
Vol 29 (5) ◽  
pp. 452-458 ◽  
Author(s):  
Bernardo Cesar Costa ◽  
Juliane Maria Guerreiro-Tanomaru ◽  
Roberta Bosso-Martelo ◽  
Elisandra Márcia Rodrigues ◽  
Idomeo Bonetti-Filho ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Cell Viability ◽  
Calcium Silicate ◽  
Setting Time ◽  
Neutral Red ◽  
Biological Properties ◽  
Alizarin Red ◽  
Ytterbium Oxide ◽  
Physicochemical And Biological Properties ◽  
Bioactive Potential

Abstract This study evaluated physicochemical properties, cytotoxicity and bioactivity of MTA Angelus (MTA), calcium silicate-based cement (CSC) and CSC with 30% Ytterbium oxide (CSC/Yb2O3). Setting time was evaluated using Gilmore needles. Compressive strength was evaluated in a mechanical machine. Radiopacity was evaluated using radiographs of materials and an aluminum scale. Solubility was evaluated after immersion in water. Cell viability was evaluated by means of MTT assay and neutral red staining, and the mineralization activity by using alkaline phosphatase activity and Alizarin Red staining. The data were submitted to ANOVA, Tukey and Bonferroni tests (5% significance). The bioactive potential was evaluated by scanning electron microscopy. The materials presented similar setting time. MTA showed the lowest compressive strength. MTA and CSC/Yb2O3 presented similar radiopacity. CSC/Yb2O3 showed low solubility. Saos-2 cell viability tests showed no cytotoxic effect, except to 1:1 dilution in NR assay which had lower cell viability when compared to the control. ALP at 1 and 7 days was similar to the control. MTA and CSC had greater ALP activity at 3 days when compared to control. All the materials present higher mineralized nodules when compared with the control. SEM analysis showed structures suggesting the presence of calcium phosphate on the surface of materials demonstrating bioactivity. Ytterbium oxide proved to be a properly radiopacifying agent for calcium silicate-based cement since it did not affected the physicochemical and biological properties besides preserving the bioactive potential of this material.

Biodentine: um substituto ao MTA?

2019 ◽  
Vol 9 (3) ◽  
pp. 29-36
Author(s):  
Maíra do Prado ◽  
Carolina Oliveira de Lima ◽  
Hugo Gonçalves Dutra ◽  
Jefferson Marion ◽  
Maria Das Graças Afonso Miranda Chaves ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Bond Strength ◽  
Physicochemical Properties ◽  
Calcium Silicate ◽  
Setting Time ◽  
Filling Material ◽  
Retrograde Filling

Objetivo: o objetivo desse estudo é realizar uma revisão de literatura em relação às propriedades físico- -químicas e biológicas do Biodentine, seu emprego na Endodontia e discutir, com base nas evidências científicas encontradas na literatura, se esse material poderá ser um substituto ao MTA. Métodos: foi realizada uma busca na literatura na base de dados PubMed, usando os termos em inglês: Biodentine, calcium silicate, MTA, properties, setting time, radiopacity, solubility, physicochemical properties, porosity, hydration, biocompatibility, bioactivity, microhardness, compressive strength, bond strength, irrigants, furcal perforation, retrograde filling material, revitalization, revascularization, endodontics, apexification. Cinquenta artigos foram incluídos. Resultados: os artigos revisados sugerem que o Biodentine tem características favoráveis e que tem alcançado resultados promissores em relação ao MTA. Conclusão: o Biodentine pode ser considerado um eventual substituto ao MTA.

scholarly journals Physicochemical Properties and Antibiofilm Activity of Tricalcium Silicate Cement and its Association with Cetrimide

2021 ◽  
pp. 333-341
Author(s):  
Giulia Bueno Rodrigues ◽  
Mario Tanomaru-Filho ◽  
Gisselle Moraima Chavez-Andrade ◽  
Fernanda Ferrari Esteves Torres ◽  
Juliane Maria Guerreiro-Tanomaru
Keyword(s):  
Physicochemical Properties ◽  
Zirconium Oxide ◽  
Setting Time ◽  
Mass Gain ◽  
Positive Control ◽  
Tricalcium Silicate ◽  
The Other ◽  
Antibiofilm Activity ◽  
Setting Times ◽  
Root Dentin

Cetrimide (CTR) is a cationic surfactant detergent with antimicrobial and antibiofilm activity. The aim of this study was to evaluate setting time, pH, solubility and antibiofilm activity of tricalcium silicate cement (TSC) with zirconium oxide (ZrO2) and its association with 0.2 and 0.4% cetrimide. Initial and final setting times (IST and FST) were assessed based on ISO-6876. pH was evaluated at periods of 1,3,7,14 and 21 days. Solubility was analyzed by weight loss. A modified direct contact test (MDCT) on the biofilm of Enterococcus faecalis formed on bovine root dentin blocks was performed, after 6 hours of manipulation and 15 hours of contact time. The analysis was performed by UFC mL ­¯¹ counting. The data were analyzed by ANOVA and Tukey’s tests (α=0.05). Higher IST was observed for TSC/ZrO2+CTR in both concentrations than for TSC/ZrO2 and lower FST for TSC/ZrO2+0.4% CTR (p<0.05). On day 1, TSC/ZrO2 showed lower pH than the associations with CTR (p<0.05). During the other periods, TSC/ZrO2 and associations promoted similar alkalinization (p>0.05). All materials exhibited increased mass. TSC/ZrO2+CTR 0.4% had lower mass gain than the other materials (p<0.05). The highest antibiofilm activity was observed for TSC/ZrO2+CTR in both concentrations, when compared with the positive control (p<0.05). In conclusion, CTR exhibited potential to promote greater antibiofilm activity to tricalcium silicate cement, without harming its physicochemical properties of setting time, pH and solubility.

scholarly journals Physicochemical Properties of Epoxy Resin-Based and Bioceramic-Based Root Canal Sealers

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Ju Kyung Lee ◽  
Sang Won Kwak ◽  
Jung-Hong Ha ◽  
WooCheol Lee ◽  
Hyeon-Cheol Kim
Keyword(s):  
Physicochemical Properties ◽  
Epoxy Resin ◽  
Setting Time ◽  
Alkaline Ph ◽  
Ph Change ◽  
Ah Plus ◽  
The One ◽  
Post Hoc ◽  
Root Canal Sealers ◽  
One Way Anova

Three bioceramic sealers (EndoSequence BC sealer, EndoSeal MTA, and MTA Fillapex) and three epoxy resin-based sealers (AH-Plus, AD Seal, and Radic-Sealer) were tested to evaluate the physicochemical properties: flow, final setting time, radiopacity, dimensional stability, and pH change. The one-way ANOVA and Tukey’s post hoc test were used to analyze the data (P=0.05). The MTA Fillapex sealer had a highest flow and the BC Sealer presented a flow significantly lower than the others (P<0.05). The BC Sealer and MTA Fillapex samples were not set in humid incubator condition even after one month. EndoSeal MTA had the longest setting time among the measurable materials and Radic-Sealer and AD Seal showed shorter setting time than the AH-Plus (P<0.05). AH-Plus and EndoSeal MTA showed statistically higher values and MTA Fillapex showed statistically lower radiopacity (P<0.05). BC Sealer showed the highest alkaline pH in all evaluation periods. Set samples of 3 epoxy resin-based sealers and EndoSeal MTA presented a significant increase of pH over experimental time for 4 weeks. In conclusion, the bioceramic sealer and epoxy resin-based sealers showed clinical acceptable physicochemical properties, but BC Sealer and MTA Fillapex were not set completely.

scholarly journals Physicochemical Properties and Volumetric Change of Silicone/Bioactive Glass and Calcium Silicate–based Endodontic Sealers

2017 ◽  
Vol 43 (12) ◽  
pp. 2097-2101 ◽  
Author(s):  
Mario Tanomaru-Filho ◽  
Fernanda Ferrari Esteves Torres ◽  
Gisselle Moraima Chávez-Andrade ◽  
Madelise de Almeida ◽  
Luciana Guilherme Navarro ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Bioactive Glass ◽  
Calcium Silicate ◽  
Volumetric Change ◽  
Endodontic Sealers

scholarly journals Solubility, Porosity, Dimensional and Volumetric Change of Endodontic Sealers

2019 ◽  
Vol 30 (4) ◽  
pp. 368-373 ◽  
Author(s):  
Fernanda Ferrari Esteves Torres ◽  
Juliane Maria Guerreiro-Tanomaru ◽  
Roberta Bosso-Martelo ◽  
Camila Galletti Espir ◽  
Josette Camilleri ◽  
...  
Keyword(s):  
Physical Properties ◽  
Dimensional Stability ◽  
Water Solubility ◽  
Total Porosity ◽  
Distilled Water ◽  
Micro Ct ◽  
Volumetric Change ◽  
Significance Level ◽  
Ah Plus ◽  
Endodontic Sealers

Abstract The aim of this study was to evaluate physical properties of endodontic sealers (AH Plus, MTA Fillapex and Endofill), by conventional and micro-CT tests. Dimensional stability was evaluated after immersion of materials in distilled water for 30 days. Solubility and volumetric change were evaluated after 7 and 30 days of immersion in distilled water. Solubility was evaluated by means of mass loss and volumetric change was assessed by micro-CT. Porosity was evaluated under a microscope after 7 days of immersion in distilled water, and by using micro-CT after setting and immersion in distilled water for 7 and 30 days. Statistical analysis was performed by ANOVA and Tukey’s test with 5% significance level. MTA Fillapex presented the highest solubility (p<0.05), showing values above the ISO/ADA recommendations. MTA Fillapex presented higher volumetric and dimensional changes, followed by Endofill and AH Plus (p<0.05). Dimensional stability of the MTA Fillapex and AH Plus did not follow ISO/ADA standards. The highest total porosity was observed for MTA Fillapex (p<0.05). Endofill had higher total porosity than AH Plus according to microscope evaluation (p<0,05), and both sealers were similar in micro-CT assessment (p>0,05). In conclusion, MTA Fillapex presented higher solubility, dimensional and volumetric change besides porosity compared to the other evaluated sealers. The assessed physical properties of sealers are related, and the different tests provided complementary data. Micro-CT is a valuable method for assessment of physical properties of endodontic materials.

scholarly journals Evaluation of the Physicochemical Properties and Push- Out Bond Strength of Mta-based Root Canal Cement

2013 ◽  
Vol 14 (6) ◽  
pp. 1094-1099 ◽  
Author(s):  
Renato de Toledo Leonardo ◽  
Gisselle Moraima Chávez-Andrade ◽  
Milton Carlos Kuga ◽  
Marco Antonio Hungaro Duarte ◽  
Katia Cristina Keine ◽  
...  
Keyword(s):  
Bond Strength ◽  
Physicochemical Properties ◽  
Root Canal ◽  
Calcium Release ◽  
Setting Time ◽  
Significance Level ◽  
Setting Times ◽  
Ah Plus ◽  
Time Test ◽  
Push Out

ABSTRACT Aim This study investigated the flowability, setting time, pH, calcium release and bond strength of a MTA-based cement (MTA Fillapex®) compared to AH Plus and Sealapex. Materials and methods For the flowability test, the ISO 6876:2001 specification was utilized and for the setting time test, the ASTM C266-03 specification was utilized. For the pH and calcium release measurements, 10 samples were prepared for each group and analyzed for several different periods. For the push-out test, dentin disks were distributed into three groups, according to the cement utilized and into three subgroups, according to the root third (n = 10). After obturation, the specimens underwent push-out testing. The data were compared statistically using a significance level of 5%. Results The flowability of all materials was found to be similar (p > 0.05). The setting times were different among the groups tested (MTA Fillapex < Sealapex < AH Plus) (p < 0.05). At days 7 and 28, the MTA Fillapex presented the higher pH values (p < 0.05). At 24 hours and at 14 days, the calcium release of the MTA Fillapex was similar to that of Sealapex (p > 0.05). AH Plus presented the lowest pH and calcium release values (p < 0.05). In all root thirds, the adhesion to the dentin of the MTA Fillapex and Sealapex were significantly lower than that of AH Plus (p < 0.05). Conclusion MTA Fillapex and Sealapex presented several similar properties and both were found to be different than AH Plus. Clinical significance This study evaluated the physicochemical and mechanical properties of new MTA-based root canal cement, in order to use this scaler in root canal fillings. MTA Fillapex showed satisfactory properties for clinical use. How to cite this article Chávez-Andrade GM, Kuga MC, Duarte MAH, de Toledo Leonardo R, Keine KC, Anna-Junior AS, Só MVR. Evaluation of the Physicochemical Properties and Push-Out Bond Strength of Mta -based Root Canal Cement. J Contemp Dent Pract 2013;14(6):1094-1099.

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